• Korean Journal of Plant Resources
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• v.8 no.1
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• pp.47-54
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• 1995

The ergot fungus requiers a rather long time for the incubation period for a disease and fungus tends to obstract the formation of the seeds. In order to do an experiment inoculated, a spore of ergot fungi into the florets was done 2-3 days before flowering. As a result, each trisomic types recognized the infection rate to be 90% to 100% so the trisomies of complete immunity was non existent in durum wheat, Triticum durum var. hordeiforme. However, the growth rate of large sclerotium than seeds differed mutually with each trisomic types. This tend to be sensitivity on the trisomies which has been the extra chromosomes of 2A, 4A and 7B in comparison with normal plant but the trisomies related to Tri-6A line was not forming the sclerotium of large size than seeds and grew outside the florets. Consequently, the resisitant gene against ergot fungi was considered to be existing on the chromosome 6A. The ergot fungi requires a long time for the incubation period until it is taken away from infection. The essential effect of resistant gene did not surmise on the chromosome 6A that gene concerned with the physiological effect to restrain the growing up of a ergot fungi in the growing process of plants.

• Korean Journal of Plant Resources
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• v.30 no.5
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• pp.571-577
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• 2017

Abscisic acid (ABA) is an important phytohormone involved in abiotic stress tolerance in plants. The group A bZIP transcription factors play important roles in the ABA signaling pathway in Arabidopsis but little is known about their functions in rice. In our current study, we have isolated and characterized a group A bZIP transcription factor in rice, OsABF3 (Oryza sativa ABA responsive element binding factor 3). We examined the expression patterns of OsABF3 in various tissues and time course analysis after abiotic stress treatments such as drought, salinity, cold, oxidative stress, and ABA in rice. Subcellular localization analysis in maize protoplasts using a GFP fusion vector further indicated that OsABF3 is a nuclear protein. Moreover, in a yeast one-hybrid experiment, OsABF3 was shown to bind to ABA responsive elements (ABREs) and its N-terminal region found to be necessary to transactivate a downstream reporter. A homozygous T-DNA insertional mutant of OsABF3 is more sensitive to salinity, drought, and oxidative stress compared with wild type plants ＆ OsABF3OX plants. In addition, this Osabf3 mutant showed a significantly decreased sensitivity to high levels of ABA at germination and post-germination. Collectively, our present results indicate that OsABF3 functions as a transcriptional regulator that modulates the expression of abiotic stress-responsive genes through an ABA-dependent pathway.

• Korean Journal of Optics and Photonics
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• v.5 no.3
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• pp.423-430
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• 1994

We designed and fabricated the single mode fiber pigtailed DFB-LD module for 2.5 Gbps optical communication system. In the design of the DFB-LD module, we made the module divided into two parts of inner sub-module and outer 14-pin butterfly package and cylindrical shaped sub-module contain quasi confocal 2 lens system including optical isolator and electrical connection between these parts via hybrid substrate of bias T circuit. Laser welding was used to assemble the sub-module which requires accurate fixing between optical elements. The fabricated DFB-LD module showed optical coupling efficiency of 20% and - 3 dB small signal response of more than 2.6 GHz. We confirmed mechanical reliability of the module by temperature cycle test where the tested module exhibit optical power fluctuation of less than 10%. Finally we evaluated the performance of the fabricated DFB-LD module as light source of 2.5 Gbps optical communication system, sensitivity of - 30.2 dBm was obtained through 47 km optical fiber transmission under the criterion of $1\times10^{-10}$ BER and transmission penalties were 1.5 dB caused by extinction ratio and 1.0 dB caused by chromatic dispersion of normal single mode fiber. fiber.

• Development and Reproduction
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• v.16 no.2
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• pp.101-106
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• 2012

Importance of behavior factors or environmental factors in visual organization and visual function of fish is treated with great care in visual ecology, and there is no study about initial ocular growth and development on the dark banded rockfish, Sebastes inermis. Thus, this study was performed. The total length, head length, head depth, eye diameter and lens diameter of the dark banded rockfish showed positive allometric relationship between parturition stage and 60 days post-parturition (dpp). The increase in total length relative to head length and head depth, head length growth relative to eye diameter and lens diameter, and head depth growth relative to eye and lens diameter were nearly isometric. The eyes were formed completely at parturtion stage. At this age, the eye has an optic nerve fiber layer, a ganglion cell layer, an inner plexiform layer, an inner nuclear layer, an outer plexiform layer, an outer nuclear layer, an outer limiting membrane, a rod and cone layer and an epithelial layer. Thickness of retina at 60 dpp was higher than that of at parturition stage. During this experiment, the proportion of the rod and cone layer, outer nuclear layer, and optic nerve fiber layer of retina were significantly increased, while the proportion of the outer plexiform layer, inner nuclear layer and ganglion cell layer of retina were significantly decreased (P<0.05). The essential demands that must be met by the retina in this species pertain to light sensitivity and spatial resolution.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.238-238
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• 2017

Salt stress is one of the deteriorating abiotic stresses due to the climate change, which causes over-accumulation of $Na^+$ and $Cl^-$ ions in plants and inhibits the growth and yield of rice especially in coastal Southeastern Asia. The yield components of rice plant (panicle number, spikelet number per panicle, 1000-grain weight, % of ripened grains) that are majorly affected by salt stress vary with growth stages at which the plant is subjected to the stress. In addition, the salt sensitivity of each yield component differs among rice varieties even when the salt-affected growth stage was same, which indicates that the physiological mechanism to maintain each yield component is different from each other. Therefore, we hypothesized that rice plant has different genes/QTLs that contribute to the maintenance of each yield component. Using a Japanese leading rice cultivar, Koshihikari, and salt-tolerant Nona bokra's chromosome segment substitution lines (CSSLs) with the genetic background of Koshihikari (44 lines in total) (Takai et al. 2007), we screened higher yielding CSSLs under salinity in comparison to Koshihikari and identified the yield components that were improved by the introgression of chromosome segment(s) of Nona bokra. The experiment was conducted in a salinized paddy field. One-month-old seedlings were transplanted into a paddy field without salinity. These were allowed to establish for one month, and then the field was salinized by introducing saline water to maintain the surface water at 0.4% salinity until harvest. The experiments were done twice in 2015 and 2016. Although all the CSSLs and Koshihikari decreased their yield under salinity, some CSSLs showed relatively higher yield compared with Koshihikari. In Koshihikari, all the yield components except panicle number were decreased by salinity and % of ripened grains was mostly reduced, followed by spikelet number per panicle and 1000-grain weight. When compared with Koshihikari, keeping a higher % of ripened grains under salinity attributed to the significantly greater yield in one CSSL. This indicated that the % of ripened grains is the most sensitive to salt stress among the yield components of Koshihikari and that the Nona bokra chromosome segments that maintained it contributed to increased yield under salt stress. In addition, growth analyses showed that maintaining relative growth rate in the late grain filling stage led to the increased yield under salt stress but not in earlier stages.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.725-731
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• 2002

This experiment was performed to understand the changes in plasma leptin in association with plasma IGF-1, body weight and ADG from early growing to late finishing stages of Holstein steers. Blood collection was performed by arterial vein puncture at selected monthly ages of 1 (54 kg), 2.6 (103 kg), 7.2 (205 kg), 13.5 (314 kg), 16.9 (414 kg), 22.2 (550 kg), 24.9 (626 kg) and 27.4 months (695 kg). The blood was analyzed for leptin using the multi-species leptin RIA with recombinant bovine leptin (rbleptin) as standard, plasma IGF-1 was also measured using RIA. Against the standard rbleptin, the multi-species Leptin RIA system's sensitivity, cross reactivity, slope and recovery of 41.0 ng/ml rbleptin in plasma were 4.9 ng/ml, 11.22%, -1.396 and 97.8%, respectively. Plasma leptin measured were more than 5.0 ng/ml, which enable multi-species RIA system to investigate plasma leptin in normal growing steers. Body weight resulted to a highly significant second-degree polynomial relationship with plasma leptin (q=0.54, p<0.0001) and plasma IGF-1 (q=0.44, p<0.0001) from 1 to 27.4 monthly ages. However, the second-degree polynomial curve of plasma leptin and IGF-1 differs showing a concave and convex curvilinear relationship, respectively. ADG was not significantly associated to plasma leptin (r=0.06, p>0.05) and plasma IGF=1 (r=0.06, p>0.05) from 1 to 27.4 monthly ages. Low coefficient, but significant associated increase of plasma leptin and IGF-1 (r=0.12, p<0.008) from 1 to 27.4 months was observed. The uncoordinated increases of plasma IGF-1 at growing and plasma leptin at fattening period, may indicate (1) indirect involvement of endogenous IGF-1 on leptin secretion, and (2) IGF-1 level may signify lean and bone accretion while plasma leptin may mirror body fatness across the monthly ages of Holstein steers.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.6C
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• pp.251-258
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• 2011

Steel pipe piles have been used as various deep foundation materials for a long time. Recent increase in steel material cost has made engineers reluctant in using it even with its good quality and ease of construction. Therefore when constructing with steel pipe pile, the decision to reuse the excessive pile length that is cut off from the designed pile head elevation after pile driving can be cost saving. This has caused many constructors to reuse the pile leftovers with new piles, but the absence of quantitative structural capacity behaviors of steel pipe pile after pile driving or appropriate countermeasures and standards in reusing steel pipe pile has resulted in wrong applications, pile structural integrity problems, inappropriate limitation of reusable pile length, etc. The structural performance analysis between a new pile and a pile that has undergone working state and ultimate state stress level during pile driving was performed in this research by means of comparing the results between the dynamic pile load test, tensile load test, charpy energy test and fatigue test for high strength steel of $440N/mm^2$ yield strength. Test results show that under working load conditions the yield strength variation is less than 2% and for ultimate load conditions the variation is less than 5% for maximum total blow count of 3000. The results have been statistically analyzed to check the sensitivity of each factors involved. From the test results, reusability of steel pipe pile lies not in the main pipe yield strength deviation but in the reduction of absorb energy, strength changes and quality control at the welded section, shape deformation and local buckling during pile driving.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.2
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• pp.124-129
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• 2016

Endotoxin, also known as lipopolysaccharide (LPS) produced by the cell wall of gram negative bacteria can be present in any liquid or on any biomaterial. Endotoxin in blood can cause fever and inflammation. In this study, we compared bacterial endotoxin using Escherichia coli O157:H7, Klebsiella oxytoca, Salmonella Typhi, Shigella sonnei and Morganella morganii. Bacteria were cultured for use in the experiment, and diluted to $1.5{\times}10^8CFU/mL$. A check marked sensitivity confirmatory test of the Limulus amebocyte lysate (LAL) reagent was performed to examine the validity. The end point reaction to each bacteria sample was confirmed with 10 fold dilution and then the final reaction end point was confirmed by 2 fold dilution between the dilution step and the upper dilution step. According to the results, in detection of endotoxins in more than 0.015 EU/mL, E. coli O157 was 75~37.5 CFU/mL, K. oxytoca 37.5~18.75 CFU/mL, M. morganii and S. Typhi 3.75~1.875 CFU/mL, and S. sonnei 7.5~3.75 CFU/mL. The resulting value was finally ensured by a confirmation test for the inhibitory factor. Based on this study, conduct of further research on bacterial endotoxin is encouraged.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.348-348
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• 2011

Carbon nanotube (CNT) field effect transistors and sensors use CNT as a current channel, of which the resistance varies with the gate voltage or upon molecule adsorption. Since the performance of CNT devices depends very much on the CNT/metal contact resistance, the CNT/electrode contact must be stable and the contact resistance must be small. Depending on the geometry of CNT/electrode contact, it can be categorized into the end-contact, embedded-contact (top-contact), and side-contact (bottom-contact). Because of difficulties in the sample preparation, the end-contact CNT device is seldom practiced. The embedded-contact in which CNT is embedded inside the electrode is desirable due to its rigidness and the low contact resistance. Fabrication of this structure is complicated, however, because each CNT has to be located under a high-resolution microscope and then the electrode is patterned by electron beam lithography. The side-contact is done by depositing CNT electrophoretically or by precipitating on the patterned electrode. Although this contact is fragile and the contact resistance is relatively high, the side-contact by far has been widely practiced because of its simple fabrication process. Here we introduce a simple method to embed CNT inside the electrode while taking advantage of the bottom-contact process. The idea is to utilize a eutectic material as an electrode, which melts at low temperature so that CNT is not damaged while annealing to melt the electrode to embed CNT. The lowering of CNT/Au contact resistance upon annealing at mild temperature has been reported, but the electrode in these studies did not melt and CNT laid on the surface of electrode even after annealing. In our experiment, we used a eutectic Au/Al film that melts at 250$^{\circ}C$. After depositing CNT on the electrode made of an Au/Al thin film, we annealed the sample at 250$^{\circ}C$ in air to induce eutectic melting. As a result, Au-Al alloy grains formed, under which the CNT was embedded to produce a rigid and low resistance contact. The embedded CNT contact was as strong as to tolerate the ultrasonic agitation for 90 s and the current-voltage measurement indicated that the contact resistance was lowered by a factor of 4. By performing standard fabrication process on this CNT-deposited substrate to add another pair of electrodes bridged by CNT in perpendicular direction, we could fabricate a CNT cross junction. Finally, we could conclude that the eutectic alloy electrode is valid for CNT sensors by examine the detection of Au ion which is spontaneously reduced to CNT surface. The device sustatined strong washing process and maintained its detection ability.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.3
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• pp.183-196
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• 2010

Increasing human activity due to rapid economic growth and land use change alters the patterns of the Asian monsoon, which is key to crop yields in Asia. In this study, we tested the performance of regional climate model (RegCM3) by simulating important components of Indian summer monsoon, including land-ocean contrast, low level jet (LLJ), Tibetan high and upper level Easterly Jet. Three contrasting rain years (1994: excess year, 2001: normal year, 2002: deficient year) were selected and RegCM3 was integrated at 60 km horizontal resolution from April 1 to October 1 each year. The simulated fields of circulations and precipitation were validated against the observation from the NCEP/NCAR reanalysis products and Global Precipitation Climatology Centre (GPCC), respectively. The important results of RegCM3 simulations are (a) LLJ was slightly stronger and split into two branches during excess rain year over the Arabian Sea while there was no splitting during normal and deficient rain years, (b) huge anticyclone with single cell was noted during excess rain year while weak and broken into two cells in deficient rain year, (c) the simulated spatial distribution of precipitation was comparable to the corresponding observed precipitation of GPCC over large parts of India, and (d) the sensitivity experiment using NIMBUS-7 SMMR snow data indicated that precipitation was reduced mainly over the northeast and south Peninsular India with the introduction of 0.1 m of snow over the Tibetan region in April.