• Title/Summary/Keyword: semi-solid medium

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Micro Fabrication Process of Powder Compact with Semi-solid Mold

  • Tsumori, Fujio
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09a
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    • pp.258-259
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    • 2006
  • New powder compaction process, in which a Bingham semi-solid/fluid mold is utilized, is developed to fabricate micro parts. In the present process, a powder material is filled as slurry in a solid wax mold, dried and compressed. The wax is heated during compaction and becomes semi-solid state, which can acts as a pressurized medium for isostatic compaction. Since the compacted micro parts are very fragile, the mold's temperature is controlled to higher than its melting point during unloading, to avoid breakage of the compacts. To demonstrate effectiveness of this process, some micro compacts of alumina are shown as examples.

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Evaluation of Absorption and Release, Survival Efficiency and Recovery Rate of Transport Medium according to the CLSI M40-A2 Standard (CLSI M40-A2 기준에 따른 수송배지의 흡수 및 배출, 생존 효율, 회수율 평가)

  • Ha, Sung-Il;Suk, Hyun-Soo;Shin, Jeong-Seob;Heo, Woong;Park, Kang-Gyun;Park, Yeon-Joon
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.2
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    • pp.164-170
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    • 2019
  • The absorption and release capacities, survival efficiency, and recovery rates of five kinds of transport media were evaluated based on the swab elution method (Quantitative) of CLSI (Clinical and Laboratory Standards Institute) M40-A2. Liquid media showed mostly better results than semi-solid media in the three evaluations. The flocked swabs had better ability to absorption and discharge bacteria than the standard swabs. The liquid medium (S4) had the best survival efficiency. Pneumococcal strains with poor growth had a higher survival efficiency and recovery rate in liquid media (S4, S5). The results of microbial recovery showed that S. pyogenes met all the CLSI standards in all media. S. pneumoniae was inadequate in the semi- solid media (S2, S3) and all the remaining media met the criteria. H. influenzae was unsuitable in semi-solid media (S1, S3) and met the criteria in semi-solid medium (S2) and liquid medium (S4, S5). The viability of the H. influenzae, pneumococcal strain causing respiratory disease, was poor in most media. Overgrowth of P. aeruginosa was observed at room temperature. The combination of liquid medium and flocked swab confirmed the best results in the three evaluation methods.

The Effect of Cultural Factors on Anther Culture in Spring Wheat (Triticum aestivum)

  • Kang, Tae-Jin;Yang, Deok-Chun
    • Plant Resources
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    • v.6 no.2
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    • pp.153-158
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    • 2003
  • Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.

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Formulations of Bacillus thuringiensis Insecticides by Liquid and Semi-Solid Fermentations. (액상 및 반고체배지 발효에 의한 Bacillus thuringiensis 살충제의 제조)

  • 이형환
    • Microbiology and Biotechnology Letters
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    • v.26 no.4
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    • pp.369-372
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    • 1998
  • Microbial insecticide formulations were prepared by liquid and semi-solid fermentations using Bacillus thuringiensis subsp. kurstaki, HL-106 (BTK-HL106), B. thuringiensis subsp. israelensis HL-63 (BTI-HL63) and B. sphaericus 1593 (BS-1593) strains. The liquid fermentation medium contained molasses 2%, dextrose 1.5%, peptone 2%, D-xylose 0.025%, CaCl$_2$ 0.1%, K$_2$HPO$_4$ 0.1%, KH$_2$PO$_4$ 0.1%, MgSO$_4$$.$7H$_2$O 0.03%, FeSO$_4$$.$7H$_2$O 0.002%, ZnSO$_4$$.$7H$_2$O 0.02%. The composition of the semi-solid fermentation medium was rice bran 45.2%, zeolite 31%, yeast powder 0.02%, corn powder 5%, dextrose 3%, lime 0.3%, NaCl 0.06%, CaCl$_2$ 0.02%, and H$_2$O 15.42%. Insecticide formulations produced in the liquid fermentation named BTK-HL106, BTI-HL63 and BS-1593 pesticides and those in the semi-solid fermentation were designated as BTK-HL106-1, BTI-HL63-1 and BS-1593-1 pesticides, respectively. The number of spore (endotoxin crystals) was 2.65${\times}$10$\^$9/ spores per $m\ell$ in the BTK-HL106 and 3.5${\times}$10$\^$10/ in the BTK-HL106-1 3.8${\times}$10$\^$9/ spores in the BTI-HL63 and 7.0${\times}$10$\^$10/ in the BTI-HL63-1, and 7.5${\times}$10$\^$9/ in the BS-1593 and 1.4${\times}$10$\^$10/ in the BS-1593-1. The spores in the BS-1593 formulation was produced two times more than the other formulations. The spores in the BTI-HL63-1 were contained twice than those in the BTK-HL106-1, and five times than those in the BS-1593-1. The results indicated that spore (endotoxin crystals) productions in the semi-solid fermentation increased about ten times than those in the liquid fermentations. $LC_{50}$s of the BTI-HL63 and BS-1593 were 4.5 $\mu\textrm{g}$, and those of the BTI-HL63-1 and BS-1593-1 were 1.5 $\mu\textrm{g}$. $LC_{50}$ of the BTK-HL106 was 1.5 mg and that of the BTK-HL106-1 was 0.9 mg. The $LC_{50}$s of the formulations in the semi-solid fermentations showed about two to three times higher than those in the liquid fermentations.

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Compression D/B for Liquid Segregation Control in Semi-Solid Forming Process and Its Application (반용융 성형공정에 있어서 액상편석제어를 위한 압축 D/B 및 응용)

  • 정경득
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1999.06a
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    • pp.15-32
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    • 1999
  • A relationship between stress and stain is very important to design a die to avoid defects of products during semi-solid forming process. Since the liquid will be of eutectic composition in alloys liquid segregation will result in significant or undesirable situation. The materials used in this experiment are A 357. A390, Al2024 alloys that is fabricated by the electro-magnetic stirring process from Pechiney in France. The compression test was performed by induction heating equipment and MTS. In order to prevent the liquid segregation these measured temperature would be useful to control of strain rate during compression test. The liquid segregation is controlled as change of the strain rate and solid fraction during the compression process, The characteristics of flow between solid and liquid phase considering liquid segregation is examined through the above experiments. In the case of medium and high volume fractions of solid the distribution of strain rate is calculated by using compression test data of semi-solid materials (SSM). The thixoforming experiments with the designed die are carried out successfully. The die filling patterns of SSM for variation of die temperature and pressing force have been investigated. The hardness of the thixoformed scroll products is evaluated in terms of the microstructure for each position.

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Induction of Multi Shoots and Plant Regeneration From Protoplasts of Alfalfa(Medicago sativa L.) (알팔파(Medicago sativa L.)의 원형질체로부터 다경 유도와 식물체의 구분화)

  • 김동명
    • Journal of Plant Biology
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    • v.32 no.4
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    • pp.313-322
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    • 1989
  • A system was established for induction of multi-shoots and plant regeneration from mesophyll protoplasts of alfalfa, Medicago sativa L. cv. Vernal. Different hormonal effects were tested at each step of protoplast culture, i.e. cell division in modified Kao's liquid medium (K566-7). calli formation on SH semi solid medium, and multi-shoot regeneration from calli on SHa and SHb solid media. Frequency of multi-shoots and plant regeneration was affected by various combinations of phytohormones in final step. The evaluation of multi-shoots induction systems via protoplast culture was discused.

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Culture and Regeneration of Populus alba × glandulosa Leaf Protoplasts Isolated from in vitro Cultured Explant (현사시나무 기내배양(器內培養) 엽육조직(葉肉組織)에서 분리(分離)된 원형질체(原形質体) 배양(培養) 및 식물체(植物体) 재분화(再分化))

  • Park, Young Goo;Son, Sung Ho
    • Journal of Korean Society of Forest Science
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    • v.77 no.2
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    • pp.208-215
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    • 1988
  • The leaf mesophyll protoplasts of Populus alba ${\times}$ glandulosa were isolated from leaf of plantlet in vitro and cultured for plant regeneration. The MS medium (minus $NH_4NO_3$) with 0.5 mg/l BAP and 2.0 mg/l 2, 4-D showed the moderate frequency of dividing protoplasts cultured by the liquid plating method during the first week of culture. The percentage of colony formation was revealed the highest frequency by the gauze contained semi-solid agar plating method after 5 weeks cultured. Ridding out the gauze, the micro-callus was formed on the same semi-solid medium in 8 weeks after protoplasts culture. For proliferation of callus, mini-callus was transferred on the MS solid medium with 0.5 mg/l 2, 4-D and 0.1 mg/l BAP 12 weeks after culture. Shoot regeneration occurred when the calli derived from protoplasts were cultured on MS medium with 1.0 mg/l zeatin and such shoots could be readily rooted on the one half strengthen MS medium with non-phytohormone. Rooting shoots were planted in green-house 22 weeks after protoplast culture.

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Process Control and Thixoforming of Cu Rotor for High Efficiency Motors (고효율 전동기용 Cu Rotor의 반응고 성형과 공정변수 제어)

  • Jung, W. S.;Lee, S. Y.;Shin, P. W.
    • Transactions of Materials Processing
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    • v.14 no.7 s.79
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    • pp.642-648
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    • 2005
  • Rotor in small-medium induction motor has been usually manufactured by aluminum diecasting. In order to improve the efficiency of induction motors, newly developed Cu-Ca alloys have been investigated. The electrical conductivity in the Cu alloys containing Ca less than $1.0wt\%$ was higher than $80\%$ IACS. Cu-Ca alloy is desirable for the thixoforming process because it has wide semi-solid range over $150^{\circ}C$. In this study, Cu-rotor with thixoforming process was developed to replace the conventional aluminum diecasting rotor. Analysis was performed for the microstructure of thixoforming rotor. Effect of incomplete filling on the efficiency of induction motor was discussed.

The Influence of Kudzu Root Starch on the Growth and Metabolism of Baker's Yeast During Aerobic Semi-Solid Fermentation (반고상 발효에서의 빵 효모 증식과 신진대사에 대한 갈근 전분의 영향)

  • 박돈희;선우창신;로버트디태너;죠지밀러니
    • Korean Journal of Microbiology
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    • v.24 no.4
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    • pp.385-388
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    • 1986
  • In a study of the aerobic growth of Baker's yeast (Saccharomyces cerevisiae) on Maxon-Johnson medium (with glucose as substrate) solidified with kudzu root starch, it was observed that between 8 and 24 hour incubation. 10 and 12% solids stimulated greater cell production than did 6 and 8% solids. The concentration of solids also affected thd secretion of protein from the yeast cells with the highest content of extracellular protein at 10-24 hour incubation stimulated by 10% starch solids.

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Process Control and Thixoforming Cu Rotor for High Efficiency Motors (고효율 전동기용 Cu Rotor의 반응고 성형과 공정변수 제어)

  • Jung W. S.;Lee S. Y.;Shin P. W.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2005.05a
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    • pp.233-236
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    • 2005
  • Rotor in small-medium induction motor has been usually manufactured by aluminum diecasting. In order to improve efficiency of induction motors, newly developed Cu-Ca alloys have been investigated. The electrical conductivity in the Cu alloys containing Ca less than $1.0wt\%$ was higher than $80\%$ IACS. Cu-Ca alloy is desirable for the thixoforming process because it has wide semi-solid range over $150^{\circ}C$. In this study, Cu-rotor with thixoforming process was developed to replace the conventional aluminum diecasting rotor. Analysis microstructure of thixoforming rotor. effect of incomplete filling defect on the efficiency of induction motor was discussed.

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