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Detection of Human Papillomavirus in Male and Female Urine by Electrochemical DNA Chip and PCR Sequencing

  • Nilyanimit, Pornjarim;Wanlapakorn, Nasamon;Niruthisard, Somchai;Pohthipornthawat, Natkrita;Karalak, Anant;Laowahutanont, Piyawat;Phanuphak, Nittaya;Gemma, Nobuhiro;Poovorawan, Yong
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.9
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    • pp.5519-5525
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    • 2013
  • Background: Cervical cancer is the second most common cancer in Thai women after breast cancer. Currently, the Papanicolaou (Pap) smear is the recommended procedure for cervical cancer screening in Thailand, but only a relatively small percentage of women follow this screening program. An alternative method to detect HPV genotypes associated with cervical cancer is self-sampling of urine, which is a more widely accepted method. Our study aimed to evaluate the prevalence of HPV in Thai women using urine and cervical swabs and prevalence of HPV in Thai men using urine samples. Materials and Methods: Tumorigenic HPV detection was accomplished by electrochemical DNA chip and PCR/direct sequencing. In addition to HPV prevalence, we report the concordance between different methods and sample types. One-hundred and sixteen women and 100 men were recruited. Histological examination revealed normal cytology in 52 women, atypical squamous cells of undetermined significance (ASCUS) in 9, low-grade squamous intraepithelial lesions (LSIL) in 24, and high-grade squamous intraepithelial lesions (HSIL) in 31. One-hundred men were classified as heterosexuals (n=45) and homosexuals (n=55). Results: The most prevalent HPV genotype in our study was HPV16. The HPV detection rate was generally lower in urine samples compared with cervical samples. Overall, there was good agreement for the detection of carcinogenic HPV from female cervical samples between the DNA chip and PCR/sequencing, with 88.8% total agreement and a kappa value of 0.76. In male urine samples, the level of agreement was higher in heterosexuals compared with homosexuals. Conclusions: Further improvement is required to increase an overall yield of HPV DNA detection in urine samples before clinical application of a urine-based HPV screening program. The electrochemical DNA chip test is a promising technique for carcinogenic HPV detection.

Cross-sectional Observation of the Sequelae of Peripheral Facial Palsy (구안와사 후유증의 분포와 인식도에 대한 단면조사)

  • Yin, Chang-shik;Kang, Mi-kyeong;Kim, Jong-deok;Hong, Jang-mu;Seo, Dong-min;Woo, Hyun-su;Lee, Hyun-jong;Ha, Ji-young;Kang, Jung-won;Park, Sang-min;Seo, Byung-kwon;Jung, In-tae;Lee, Sang-hoon;Koh, Hyung-kyun
    • Journal of Acupuncture Research
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    • v.20 no.3
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    • pp.24-33
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    • 2003
  • Background : Sequelae symptoms of peripheral facial palsy include not only partial recovery of the paretic muscles but contracture, spasm, synkinesis and atrophy which cause significant functional, esthetic, and psychosocial disturbances to the lives of patients. Objective : This study is to investigate the prevalence, time of onset, patient's self assessment on the degree of understanding of the sequelae and the association of the degree of palsy with the appearance of sequelae. Methods : 106 patients with peripheral facial palsy were sequentially interviewed and examined. Results : 29 patients(27.4%) of 106 patients showed sequelae symptoms, of whom 19 patients(65.5%) showed sequelae symptoms during 4 to 6 months after the onset of palsy. The degree of understanding on the sequelae at the time of interview showed improvement compared to that at the time of first consultation to any physician but not to reach an sufficient understanding. No relation between the degree of palsy and the appearance of sequelae was observed.

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Evaluation of flexural performance of high performance fiber reinforced cementitious composites according to fiber shape, aspect ratio and volume fraction (강섬유의 형상, 길이 및 혼입율에 따른 고성능 섬유보강 시멘트 복합체의 휨 특성 평가)

  • Park, Gi-Joon;Park, Jung-Jun;Kim, Sung-Wook;Lee, Jang-Hwa
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.12
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    • pp.697-704
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    • 2017
  • High-Performance Fiber-Reinforced Cement Composites (HPFRCC) has outstanding durability, and has attracted interest because of its ductility and development of strength, which allows a reduction of the self-weight of a structural member by substantially decreasing the cross section. Therefore, the present study aimed to improve the economic efficiency of HPFRCC by examining experimentally the flexural performance considering various characteristics of the steel fiber. To find an efficient fiber reinforcement method, the flexural performance was evaluated for different shapes, aspect ratios, and volume ratios of the steel fiber. Straight, hooked, and twisted fiber configurations were considered by adopting a fiber length longer than the usual 13 mm. The test results showed that HPFRCC reinforced by 19.5 nun-long straight fibers with a volume fraction of 1.5% shows better flexural performance than that reinforced by 13 mm-long straight fibers with a volume fraction of 2.0%. Consequently, HPFRCC with enhanced economic efficiency can be produced by adopting a reduced amount of steel fiber.

Risk Factors Associated with Loneliness in the Elderly Living Alone (독거노인의 외로움에 영향을 미치는 위험요인)

  • Lee, Seung Woo;Paik, Ki Chung;Lee, Kyung Kyu;Lee, Seok Bum;Kim, Kyung Min;Kim, Dohyun;Lee, Jung Jae
    • Korean Journal of Psychosomatic Medicine
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    • v.27 no.2
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    • pp.173-180
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    • 2019
  • Objectives : Loneliness is associated with negative mental and physical health. However, little is known about the risk factors of loneliness in the Korean elderly living alone. The aim of this study was to examine sociodemographic and social network related risks for loneliness among the elderly living alone. Methods : This is a cross-sectional study that enrolled 1,091 subjects who are the community-residing elderly living alone. Sociodemographic status, medical condition, cognition, mood disorder and levels of loneliness were collected using a self-administered questionnaire and a specific semi-structured interview conducted by a trained nurse. Descriptive statistics were used to analyze data regarding sociodemographic variable and loneliness. Univariate and Multivariate regression analyses were applied to examine factors associated with loneliness. Results : The mean score of loneliness was 3.8 (SD=1.7). No family contact (standardized β=0.115, p<0.001), no religious attendance (standardized β=0.057, p=0.028), no gathering with friends (standardized β=0.088, p=0.001) and high score of Short for of Geriatric Depression Scale (standardized β=0.502, p<0.001) were significantly associated with high loneliness in the elderly living alone. Conclusions : Family function, social network and depressive mood could be significant risk factors for high loneliness in the elderly living alone. Public health promotion efforts to reduce loneliness should focus on improving family function, social network and decreasing depression.

A Study on Weight-Control Experience, Eating Disorder and Nutrient Intake of College Students Attending Web Class via the Internet (인터넷 Web 강의를 수강하는 남녀 대학생에서의 체중조절 경험, 섭식장애 및 식이섭취 실태에 관한 연구)

  • 이정희;김진숙;이미영;정선희;장경자
    • Korean Journal of Community Nutrition
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    • v.6 no.4
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    • pp.604-616
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    • 2001
  • This study was to investigate weight-control experience disorder and nutrient intake of college student attending web class via the internet. The subject were 1200 college students(392 male, 808 female) attending web class concerned with nutrition and weight control. This cross-sectional survey was conducted by self-administered questionnaire and data were analyzed by Students t-test and $\chi$$^2$-test. Nutrient intake collected from 3 day-recalls were analyzed by the Computer Aided Nutritional Analysis Program. Mean height, weight and RBW(relative body weight) of the male college students were 174.7 cm, 68.0 kg and 101.2%, respectively. Mean height, weight and RBW of the female college students were 162.3% cm, 52.7% kg and 95.3% respectively. Among the male college students, low weight were 1.0%, underweight were 12.8%, overweight were 12.5%, obese were 6.9% by RBW standard. Among the female college students, low weight were 5.1%, underweight 30.2%, overweight were 5.2%, obese were 1.5% by RBW standard. Percentages of weight control experience were 21.7% and 67.0% in the male and female college students, respectively. According to RBW, 22.4% of the weight control experience group were low weight or underweight in the female college students but none of weight control experience group were low weight or underweight in the male college students. Percentage of eating disorder were 6.8%(2.8%, male, 8.7% female) in the college students. Among the female college students, most nutrient intakes were significantly lower in the weight-control experienced group compared to the weight-control nonexperienced group. Among the male college students, there was a tendency that the intake of nutrients, except carbohydrate, calcium and vitamin A was higher in the eating disorder group compared to the non-eating disorder group. However, among the female college students, there was a tendency that the intake of nutrients, except calcium and thiamin was lower in the eating disorder group compared to the non-eating disorder group. Therefore, nutrition education via the internet is required for college students to know proper weight loss methods, desirable eating habits and eating disorder problem well.

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A study on the implementation of infection control at dental offices (치과 진료실 감염방지 실천에 관한 연구)

  • Woo, Seung-Hee;Kwag, Jung-Suk;Ju, On-Ju;Lim, Kun-Ok
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.3
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    • pp.282-293
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    • 2009
  • The purpose of this study was to examine the degree of infection control implemented at dental offices and factors affecting it in an attempt to help promote the health of dental health care workers. The subjects in this study were 180 medical personnels who worked at dental offices in the region of South Jeolla Province. A self-administered survey was conducted from April 1 to May 30, 2008, and the collected data were analyzed. The findings of the study were as follows: 1. As for the implementation of infection control at the dental offices, what the health care workers investigated did the most was post-treatment hand washing(95.0), a constant separation of infectious wastes(94.4), wearing rubber gloves all the time during medical instrument cleansing(92.8) and pre-treatment hand washing(91.7). 2. In regard to the implementation of infection control at the dental offices, what the dental personnels did the least was drying their hands with air(5.0), wearing goggles in times of treatment(23.3), receiving regular education on infection control(26.7) and putting sterilizers to a performance test on a regular basis(43.9). 3. The dental health care workers were significantly different according to age in the management of contagious diseases(p=0.005). Their career made a significant difference to the management of contagious diseases(p=0.000) and instrument cleansing/sterilization(p=0.043). The service area made a significant difference to wearing and managing personal protective clothes (p=0.040) and waste management(p=0.040). 4. Concerning the relationship between the acquisition of dental hygienist certificate and the practice of infection control, whether the dental health care workers were certified or not made no significant difference to that. 5. As to the correlation among the factors affecting the prevention and management of contagious diseases, there was a positive correlation among hand washing(r=0.379), wearing and managing personal protective clothes(r=0.349), instrument cleansing/sterilization(r=0.323) and waste management(r=0.388). All the factors made a statistically significant difference to the prevention and management of contagious diseases(p<0.01).

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Alcohol Craving in Subjects with Alcohol Use Disorders in Response to Alcohol Cues (알코올 사용 장애자의 알코올 단서에 의해 유발된 갈망 특성)

  • Park, Mi-Sook;Sohn, Sun-ju;Park, Ji-Eun;Kim, Suk-Hee;Yu, In-Kyu;Sohn, Jin-Hun
    • Science of Emotion and Sensibility
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    • v.10 no.4
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    • pp.603-611
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    • 2007
  • Initially, this study was to develop reliable and effective alcohol-associated visual cues. Alcoholic picture cues were chosen from the Normative Appetitive Picture System (NAPS). Additional images consisted of Korean beer and mild liquor was pre-tested to select the pictures that would induce craving most intensely. The images that recorded the highest scores on a scale were chosen through the pre-test. And then, the reliability and validity were examined for the selected alcohol cues from NAPS and pre-test via another psychometric test. Secondly, the study was to investigate differences in craving between subjects with alcohol use disorders (AUDs) and non-alcoholic control subjects when exposed to the alcohol visual cues. Alcohol abusers with AUD (n=9) and demographically similar non-abusers (n=9) participated in this study. After given 5cc of alcohol, subjects were exposed to different types of stimuli (i.e., alcohol, nonalcoholic beverage, and visual control pictures and one rest (cross-hair)). Craving levels were rated through self-report on a Likert scale immediately after the presentation of visual cues. Results showed statistically significant differences between the AUD group and the control group in the Obsessive Compulsive Drinking Scale (OCDS) scores, pre-sip and post-sip alcohol craving. Also, the AUD group showed significantly a higher level of craving during alcohol cues compared to the control group. In conclusion, alcohol craving induced by alcohol cues among subjects with AUD was found to be different from that of non-abusers.

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Breeding of a New Japanese Apricot (Prunus mume Siebold et Zucc.) Cultivar 'Okjoo' with High Yields (다수성 매실 품종 '옥주')

  • Kim, Yoon-Kyeong;Kang, Sam-Seok;Choi, Jang-Jeon;Cho, Kwang-Sik;Won, Kyeong-Ho;Lee, Han-Chan;Choi, Jin-Ho
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.912-916
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    • 2014
  • Japanese apricot (Prunus mume Siebold and Zucc.) is a deciduous tree of the family Rosaceae, and it has long been used as a folk remedy for cough and dyspepsia. A new cultivar 'Okjoo' was developed from a cross between 'Gyokuei' and 'Rinsyu' carried out at the National Institute of Horticultural & Herbal Science in 1993. It w as s elected for good shape, large size and high yield capacity in 2006, and then it was granted official patent No. 4556 in 2013. It blooms 4 days and 2 days earlier than 'Gyokuei' and 'Rinsyu', respectively. Its flower petal color is pink, and the pollen amount is negligible. Its S-genotype, determined using Polymerase Chain Reaction with a S-RNase gene-specific primer pair, is $S_3S_6$. The average optimum harvest time of 'Okjoo' is late June. The fruit is round in shape and its suture is shallow. Average fruit weight is 18.5 g, and it contains total soluble solids $7.66^{\circ}Brix$ and titratable acidity at 4.81%. Fruit skin color is green. Sometimes only the light side of the fruits seems to develop blush. The incidence levels of scab (Cladosporium carpophilum Thumen) and bacterial shot hole (Xanthomonas arboricola pv. Pruni) are quite low. Consequently, 'Okjoo' seems to be a promising new cultivar for Japanese apricot growers.

Associations of Eating Habits with Obesity and Nutrition Knowledge for Middle and High School Adolescents in Shanghai and Heze China (중국 상하이·허쩌 중·고등학생의 식습관과 비만도 및 영양지식과의 관련성 연구)

  • Song, Yang;Ahn, Hyo-Jin;Choi, Ji-Hye;Oh, Se-Young
    • Journal of the Korean Society of Food Culture
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    • v.29 no.6
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    • pp.648-658
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    • 2014
  • The aim of this study was to investigate the relationships between eating habits and health among adolescents in Shanghai and Heze, China. A cross-sectional study was conducted in 2013 on 2,089 adolescents; 1,089 students were from Shanghai and 999 students from Heze region. Eating habits, weight, height, and nutritional knowledge were assessed using a self-administered questionnaire. Eating habits score was classified into two categories: healthy eating habits and unhealthy eating habits, based on "Korean Youth Risk Behavior Web-based Survey", for statistical data analysis. Associations between eating habits, BMI, and nutritional knowledge were examined using a general linear model with adjustment of potential confounding factors such as region, gender, age, parents' education level, and pocket money. Statistical analyses were performed using the SAS (version 9.3) program. Proportions of healthy eating habits group were 90.0% for breakfast (3-7 times/wk), 29.1% for fruit (${\geq}once/d$), 12.5% for vegetable (${\geq}3times/d$), 7.3% for milk (${\geq}2times/d$), 90.0% for fast food (<3 times/wk) consumption, respectively. The average BMI score was 20.1 (Shanghai 20.5 Heze 19.6), which is in the range of normal weight. Rates of obesity and overweight were 16.5% and 8.3% in Shanghai and Heze, respectively. There were significant negative correlations between intake frequencies of breakfast, fast food, biscuits, sugar, chocolate, and BMI score. Eating habits and nutritional knowledge score showed a significant positive correlation. These results showed better eating habits regarding eating regularity and consumption of fruits and soft drinks in Chinese adolescents compared with Korean adolescents, although cultural differences were not fully considered. This study demonstrated significant associations of BMI and nutritional knowledge with dietary behavior in Chinese adolescents in two regions of China. Further studies on Chinese adolescents from other regions in China should be considered.

Respiratory Health of the Children Living near the Petrochemical Estate in Ulsan (울산 석유화학공단 인근 어린이들의 호흡기 건강상태)

  • Yoo, Cheol-In;Lee, Ji-Ho;Kim, Yang-Ho;Lee, Choong-Ryeol
    • Journal of Preventive Medicine and Public Health
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    • v.33 no.2
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    • pp.174-183
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    • 2000
  • Objectives : To evaluate the effect of low-level exposure of air pollutants on the respiratory tract of the children living near the petrochemical estate in Ulsan. Methods : The study design was cross-sectional, and the study subjects consisted of 150 children(76 boys, 74 girls) living near the petrochemical estate and 100 children(53 boys, 47 girls) living in a suburban area. We investigated respiratory health using self-administered questionnaires(ATS-DLD-78) , radiological examination, and pulmonary function test such as FVC and FEV1. Results : There were higher prevalence rates of respiratory symptoms in the children living near the petrochemical estate than the children living in a suburban area. And the results of FVC and FEV1 of 11-years old children living near the petrochemical estate were lower than those of the children living in a suburban area. Conclusions : Chronic exposure of low-level air pollutants would affect respiratory health of the children. Therefore, further a longitudinal study of respiratory health will be needed for children living near the petrochemical estate in Ulsan.

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