• Title/Summary/Keyword: self -assembly

Search Result 690, Processing Time 0.03 seconds

Real-Time Detection of DNA Hybridization Assay by Using Evanescent Field Microscopy

  • Kim, Do-Kyun;Choi, Yong-Sung;Murakami, Yuji;Tamiya, Eiichi;Kwon, Young-Soo
    • KIEE International Transactions on Electrophysics and Applications
    • /
    • v.11C no.3
    • /
    • pp.85-90
    • /
    • 2001
  • The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, the improvement of DNA detection system is very important for the determination of this hybridization reaction. In this study, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. First, we have fabricated DNA chip microarray. The particles which were immobilized oligonucleotides were arranged by the random fluidic self-assembly on the pattern chips, using hydrophobic interaction. Second, we have detected DNA hybridization reaction using evanescent field microscopy. The 5'-biotinylated probe oligonucleotides were immobilized on the surface of DNA chip microarray and the hybridization reaction with the Rhodamine conjugated target oligonucleotide was excited fluorescence generated on the evanescent field microscopy. In the foundation of this result, we could be employed as the basis of a probe olidonucleotide, capable of detecting the target oligonucleotide and monitoring it in a large analyte concentration range and various mismatching condition.

  • PDF

Identification of the Mechanical Resonances of Electrical Drives for Automatic Commissioning

  • Pacas Mario;Villwock Sebastian;Eutebach Thomas
    • Journal of Power Electronics
    • /
    • v.5 no.3
    • /
    • pp.198-205
    • /
    • 2005
  • The mechanical system of a drive can often be modeled as a two- or three-mass-system. The load is coupled to the driving motor by a shaft able to perform torsion oscillations. For the automatic tuning of the control, it is necessary to know the mathematical description of the system and the corresponding parameters. As the manpower and setup-time necessary during the commissioning of electrical drives are major cost factors, the development of self-operating identification strategies is a task worth pursuing. This paper presents an identification method which can be utilized for the assisted commissioning of electrical drives. The shaft assembly can be approximated as a two-mass non-rigid mechanical system with four parameters that have to be identified. The mathematical background for an identification procedure is developed and some important implementation issues are addressed. In order to avoid the excitation of the system with its natural resonance frequency, the frequency response can be obtained by exciting the system with a Pseudo Random Binary Signal (PRBS) and using the cross correlation function (CCF) and the auto correlation function (ACF). The reference torque is used as stimulation and the response is the mechanical speed. To determine the parameters, especially in advanced control schemes, a numerical algorithm with excellent convergence characteristics has also been used that can be implemented together with the proposed measurement procedure in order to assist the drive commissioning or to achieve an automatic setting of the control parameters. Simulations and experiments validate the efficiency and reliability of the identification procedure.

Microcantilever-based biosensor using the surface micromachining technique (표면 미세 가공기술을 이용한 마이크로 캔틸레버의 제작과 바이오션서로의 응용)

  • Yoo, Kyung-Ah;Joung, Seung-Ryong;Kang, Chi-Jung;Kim, Yong-Sang
    • Proceedings of the KIEE Conference
    • /
    • 2005.07c
    • /
    • pp.2407-2409
    • /
    • 2005
  • 본 논문에서는 다양한 생물분자 감지를 위한 센서로 마이크로캔틸레버를 제안하였고 이것을 이용해 여러 생물 분자들을 광학적, 전기적으로 분석하였다. 마이로캔틸레버는 표면 미세 가공 기술로 제작되었고, 이러한 제작 방식은 공정이 간단하고 비용이 적게 들며 센서 array가 가능하다는 장점을 갖는다. 생물분자를 포함하는 용액을 주입하기 위하여 PDMS와 fused silica glass를 이용해 fluid cell system을 제작하였다. 마이크로캔틸레버 상단의 gold가 코팅된 부분에서 생물분자의 자기조립 (self assembly)현상이 일어나고 이는 마이크로캔틸레버 상, 하단의 표면 스트레스 차이를 야기 시킨다. 이로 인해 마이크로캔틸레버 자체의 휘어짐 현상이 일어나게 되고 이러한 휘어진 정도를 측정함으로써 마이크로캔틸레버의 생물분자 감지능을 확인할 수 있었다. Cystamine dihydrochloride와 glutaraldehyde 분자를 분석하였고 각기 다른 농도의 cystamine dihydrochloride 용액에서도 실험함으로써 농도별 감지능 또한 확인하였다. 이러한 생물분자 감지를 위한 마이크로캔틸레버의 센서로써의 성능은 u-TAS 와 lab-on-a-chip에서 유용히 이용될 수 있으리라 확신한다.

  • PDF

Multidimensional ZnO light-emitting diode structures grown by metalorganic chemical vapor deposition on p-Si (이종접합구조를 이용한 다층형복합구조의 산화아연 발광소자 제작)

  • Kim, Dong-Chan;Han, Wan-Suk;Kong, Bo-Hyun;Cho, Hyung-Koun;Kim, Hyoung-Sub
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2007.11a
    • /
    • pp.59-59
    • /
    • 2007
  • 최근 GaN계 LED를 대체할 만한 물질로 주목받고 있는 ZnO는 단결정 박막성장의 어려움, 동일접합 LED 소자구현을 위한 p-ZnO 성장의 어려움 3원계 합금제작의 어려움 등으로 소자제작에 있어 고전을 하고 있다. 특히 이러한 문제점을 극복하고자 하는 방안으로 양자 제한 효과, 탁월한 결정성, self-assembly, internal stress 등의 새로운 기능성을 지닌 ZnO 나노구조가 제시되었다. 하지만 나노구조를 이용한 다이오드 제작에서도 금속전극의 접합이라는 문제의 벽에 가로막혀 있다. 본 실험에서는 자체 개발된 MOCVD 장비를 이용한 일차원 ZnO 나노선을 성장한 이후 연속적으로 박막을 성장하여 금속전극의 접합을 시도하였다. 이종접합구조 뿐만 아니라 일차원 및 이차원 구조의 복합구조는 일반 다결정 박막보다 결정성에서 우수한 특성을 보였으며, 다이오드 제작시에 높은 효율을 보였다.

  • PDF

Local Electronic Structures of Graphene Probed by Scanning Tunneling Spectroscopy

  • Jang, Won-Jun;Lee, Eui-Sup;Kim, Howon;Yoon, JongKeon;Chang, Yunhee;Kim, Yong-Hyun;Kahng, Se-Jong
    • Proceedings of the Korean Vacuum Society Conference
    • /
    • 2013.08a
    • /
    • pp.132.2-132.2
    • /
    • 2013
  • Electrons in graphene make ballistic transport with very high mobility (${\sim}2{\times}105 $cm2V-1s-1), which holds promises for applications in fast electronic devices. However, such expectations have been hampered by the semi-metallicity or zero bandgap of graphene, which makes it impossible to completely turn off graphene transistor devices. Here, we report the observations of local bandgap modulations in Moir$\acute{e}$ patterned graphene on metal substrates using scanning tunneling microscopy and spectroscopy. The Moir$\acute{e}$ patterned graphene was made by combinations of self-assembly processes, and they showed additional electronic states that could be interpreted as sub-band states. Our experimental observations could be explained with orbital transitions of carbon atoms from sp2 to sp3, as supported by our density functional theory calculation results. Our findings will add new poweful components for device applications.

  • PDF

Ordered Micropatterns by Confined Dewetting of an Imprinted Polymer Thin Film and Their Microlens Application

  • Lee, Geun-Tak;Yoon, Bo-Kyung;Acharya, Himadri;Park, Cheol-Min;Huh, June
    • Macromolecular Research
    • /
    • v.17 no.3
    • /
    • pp.181-186
    • /
    • 2009
  • We fabricated ordered micro/nano patterns induced by controlled dewetting on the topographically patterned PS/P4VP bilayer thin film. The method is based on utilizing microimprinting lithography to induce a topographically heterogeneous bilayer film that allows the controlled dewetting upon subsequent thermal annealing. The dewetting that was initiated strictly at the boundary of the thicker and thinner regions was guided by the presence of the topographic structure. The dewetting front velocity of the microdomains in the confined regions was linearly proportional to the measurement time, which enabled us to control the size of the dewet domain with annealing time. In particular, the submicron sized dot arrays between lines were generated with ease when the dewetting was confined into geometry with a few microns in size. The kinetically driven, non-lithographical pattern structures accompanied the pattern reduction to 400%. The pattern arrays on a transparent glass substrate were especially useful for non-circular microlens arrays where the focal length of the lens was easily tunable by controlling the thermal annealing.

Polarity Index Dependence of M13 Bacteriophage-based Nanostructure for Structural Color-based Sensing

  • Lee, Yujin;Moon, Jong-Sik;Kim, Kyujung;Oh, Jin-Woo
    • Current Optics and Photonics
    • /
    • v.1 no.1
    • /
    • pp.12-16
    • /
    • 2017
  • Color sensor systems based on M13 bacteriophage are being considerably researched. Although many studies on M13 bacteriophage-based chemical sensing of TNT, endocrine disrupting chemicals, and antibiotics have been undertaken, the fundamental physical and chemical properties of M13 bacteriophage-based nanostructures require further research. A simple M13 bacteriophage-based colorimetric sensor was fabricated by a simple pulling technique, and M13 bacteriophage was genetically engineered using a phage display technique to exhibit a negatively charged surface. Arrays of structurally and genetically modified M13 bacteriophage that can determine the polarity indexes of various alcohols were found. In this research, an M13 bacteriophage-based color sensor was used to detect various types of alcohols, including methanol, ethanol, and methanol/butanol mixtures, in order to investigate the polarity-related property of the sensor. Studies of the fundamental chemical sensing properties of M13 bacteriophage-based nanostructures should result in wider applications of M13 bacteriophage-based colorimetric sensors.

Effect of nano-carbon addition on color performance of polystyrene superstructure film

  • ZHOU, Ye-min;Wang, Li-li;LI, Xiao-peng;Wang, Xiu-feng;Jiang, Hong-tao
    • Journal of Ceramic Processing Research
    • /
    • v.19 no.6
    • /
    • pp.479-482
    • /
    • 2018
  • Polystyrene superstructure films show faint rainbow color, and this low color saturation limits its wide application. In this paper, polystyrene superstructure films with single bright blue color were prepared by vertical deposition self-assembly method using polystyrene microspheres with average diameter of $310{\pm}10nm$ as raw material. Polystyrene superstructure films were modified by adding nano-carbon powder, and effect of the amount of nano-carbon powde on color performance was studied. The results showed that without addition of nano-carbon powder, the superstructure films showed a faint rainbow color, while with addition of nano-carbon power, the superstructure films exhibited a single bright blue under the same natural light source. Changing the amount of nano-carbon powder addition could adjust color saturation of the film. With increasing the amount of nano-carbon powder addition from 0.008 wt% to 0.01 wt%, color saturation of the superstructure film increased gradually. Further increasing the amount of nano-carbon powder addition to 0.011wt%, color saturation of the superstructure film didn't increase anymore and tended to get dark.

A Study on Polymer Replica Materials for Nanotransfer Printing (패턴전사프린팅용 고분자 복제 소재 연구)

  • Kang, Young Lim;Park, Woon Ik
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.34 no.4
    • /
    • pp.262-268
    • /
    • 2021
  • For the past several decades, various next-generation patterning methods have been developed to obtain well-designed nano-to-micro structures, such as imprint lithography, nanotransfer printing (nTP), directed self-assembly (DSA), E-beam lithography, and so on. Especially, nTP process has much attention due to its low processing cost, short processing time, and good compatibility with other patterning techniques in achieving the formation of high-resolution functional patterns. To transfer functional patterns onto desirable substrates, the use of soft materials is required for precise replication of master mold. Here, we introduce a simple and practical nTP method to create highly ordered structures using various polymeric replica materials. We found that polymethyl methacrylate (PMMA), polystyrene (PS), and polyvinylpyridine (PVP) are possible candidates for replica materials for reliable duplication of Si master mold based on systematic analysis of pattern visualization. Furthermore, we successfully obtained well-defined metal and oxide nanostructures with functionality on target substrates by using replica patterns, through deposition and transfer process. We expect that the several candidates of replica materials can be exploited for effective nanofabrication of complex electronic devices.

Recent Advances in Soft Magnetic Actuators and Sensors using Magnetic Particles (자성 분말 기반 소프트 자성 액츄에이터 및 센서 연구 동향)

  • Song, Hyeonseo;Lee, Hajun;Kim, Junghyo;Kim, Jiyun
    • Journal of Powder Materials
    • /
    • v.28 no.6
    • /
    • pp.509-517
    • /
    • 2021
  • Smart materials capable of changing their characteristics in response to stimuli such as light, heat, pH, and electric and magnetic fields are promising for application to flexible electronics, soft robotics, and biomedicine. Compared with conventional rigid materials, these materials are typically composed of soft materials that improve the biocompatibility and allow for large and dynamic deformations in response to external environmental stimuli. Among them, smart magnetic materials are attracting immense attention owing to their fast response, remote actuation, and wide penetration range under various conditions. In this review, we report the material design and fabrication of smart magnetic materials. Furthermore, we focus on recent advances in their typical applications, namely, soft magnetic actuators, sensors for self-assembly, object manipulation, shape transformation, multimodal robot actuation, and tactile sensing.