• Asian-Australasian Journal of Animal Sciences
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• 제22권12호
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• pp.1661-1666
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• 2009

The objective of this study was to determine the effect of Se-enriched bean sprout, Se-enriched yeast and sodium selenite on productivity, egg quality and egg Se concentrations in laying hens. Using a Completely Randomized Design, 144 Rohman laying hens at 71 weeks of age were divided into four groups. Each group consisted of four replicates and each replicate contained nine hens. The dietary treatments were T1: control diet, T2: control diet plus 0.3 mg Se/kg from sodium selenite, T3: control diet plus 0.3 mg Se/kg from Se-enriched yeast, T4: control diet plus 0.3 mg Se/kg from Se-enriched bean sprout. The results showed that there was no significant difference (p>0.05) in feed intake, egg production and egg quality among treatments. Selenium supplementation from Seenriched yeast and Se-enriched bean sprout markedly increased (p<0.05) egg Se concentration as compared to the control and sodium selenite groups. The results indicated that Se-enriched bean sprout could be used as an alternative Se source in diets of laying hens.

• Asian-Australasian Journal of Animal Sciences
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• 제19권2호
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• pp.217-223
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• 2006

Fifty castrated crossbred ($Landrace{\times}Yorkshire$) pigs, weighing an average of $60.6{\pm}3.1kg$ were allotted to one of five treatments in a randomized block design to examine the effects of dietary inclusion of 0.1% L-carnitine (50 ppm carnitine), 0.1% selenium-enriched yeast (0.3 ppm selenium), 0.1% Jujube fruit or 0.1% Hwangto (Red clay) on pig performance and carcass quality. All diets were based on corn, wheat, soybean meal and wheat bran and were formulated to supply 13.8 MJ DE/kg. Dietary supplementation did not influence daily gain (p = 0.57), feed intake (p = 0.52), or feed conversion (p = 0.32). Digestibility of dry matter (p = 0.60), organic matter (p = 0.74), crude protein (p = 0.76), crude fibre (p = 0.70) and energy (p = 0.75) were also unaffected by inclusion of any of the additives. Tissue samples taken from the longissimus muscle showed that the levels of carnitine (p = 0.0001) and selenium (p = 0.0001) were significantly higher with dietary inclusion of carnitine or selenium-enriched yeast. Dietary treatment did not affect dressing percentage (p = 0.33), carcass lean yield (p = 0.99) or first, $10^{th}$ and last rib midline backfat depth (p = 0.45, 0.82 and 0.47, respectively). Dietary treatment also did not affect the percentages of tenderloin (p = 0.37), bacon (p = 0.36), fat and bone (p = 0.56), picnic shoulder (p = 0.25), skirt (p = 0.80), fresh ham (p = 0.31) or ribs (p = 0.79). However, pigs fed the diet containing Jujube fruit had a higher percentage of Boston butt than pigs fed the carnitine or selenium supplemented diets (p = 0.01). Pigs fed added Hwangto had a higher (p = 0.04) percentage of loin compared with pigs fed supplementary selenium or Jujube fruit. Loin muscle from pigs fed carnitine had a significantly lower Hunter colour value for L (whiteness, p = 0.004) and a higher value for $a^*$ (redness; p = 0.069). The overall results indicate that supplementation with L-carnitine and selenium-enriched yeast can produce pork containing higher levels of carnitine and selenium, which could provide health benefits for consumers of pork without detrimental effects on pig performance.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2002년도 춘계학술대회
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• pp.146-146
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• 2002

• 대한지역사회영양학회지
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• 제10권5호
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• pp.700-707
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• 2005

The purpose of this study was to investigate the implications of dietary intake and the level of serum micronutrients (Se, Mn, Cu, Zn), lipid peroxidation, and antioxidant capacity in Korean women with cervical intraepithelial neoplasia (CIN) . From October 2002 to March 2003, 50 patients diagnosed with CIN (confirmed with colposcopy directed biopsy) and 50 patients without any cervical disease as the control group were enrolled in the study at the Department of Gynecology Cancer Center at Samsung Cheil Hospital. Animal fat intake in CIN group was significantly higher than that of the control group, but plant protein intake in the CIN group was significantly lower than that of the Control group. Energy and zink intakes were similar in the two groups. The serum concentration of antioxidant minerals in the CIN group were not significantly different from the control group. The total radical trapping antioxidant potential concentration of plasma was significantly lower in the CIN group (1.12mM) than in the control group (1.25mM) (p<0.05). But MDA (malondialde-hyde) of serum was significantly higher in the CIN group (7.60mM) than in the control group (4.99mM) (p<0.005). The serum selenium concentration of the control group showed significant positive correlation with the MDA level (r=0.311). These findings are suggestive of protective roles for healthy dietary habit including increasing antioxidant nut-rients and decreasing intake of fat.

• Asian-Australasian Journal of Animal Sciences
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• 제23권7호
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• pp.931-936
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• 2010

This experiment was conducted to evaluate the bioavailability of different organic selenium (Se) products in finishing pigs. A total of 48 growing pigs, average body weight $47.6kg{\pm}0.05$, were allotted to four different treatments in a randomized complete block (RCB) design in three replicates with four pigs per pen. Three different organic Se products, Se-enriched yeast (treatments A and B) and Se-proteinate (treatment C), were used in conjunction with a basal diet with no added Se as a control treatment. In growing period, pigs were fed the same diet but finishing pigs were fed each treatment diet containing organic Se products for 6 weeks. During the experimental period, feed intake and body weight were measured and blood samples were collected to determine the Se concentration. At the end of this experiment, 3 pigs per treatment were killed and various tissues (loin, liver, kidney, pancreas and spleen) were collected to analyze the Se concentration. The body weight, and average daily feed intake (ADFI) were similar among treatments, but the average daily gain (ADG) was increased on Se-proteinate treatment (p<0.01) and gain-to-feed ratio (G/F ratio) was improved on Se yeast B or Se-proteinate treatment (p<0.01). The tissue Se content was also increased when pigs were fed organic Se sources, and Se was retained efficiently in loin (p<0.01) and kidney (p<0.05) when Se yeast B was provided. The serum Se concentration was increased when organic Se was provided and was higher when pigs were fed Se-proteinate (p<0.01); subsequently liver Se was also higher on Se-proteinate treatment than other treatments. The Se yeast A treatment did not show any increment of Se concentration both in serum and tissues. This result demonstrated that Se retention and bioavailability in finishing pigs were varied by Se products although organic sources were provided. Consequently, each organic Se product should be evaluated before it is used as a supplement in animal feed.

• 현장농수산연구지
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• 제7권1호
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• pp.97-108
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• 2005

본 연구는 사료내 적정 셀레늄수준(0.9ppm)에서 Se-SMC(유기셀레늄강화버섯폐배지)의 급여사양기간(2개월, 4개월, 6개월)을 달리 했을 때, 비육후기 한우의 조직 내 셀레늄함량에 미치는 영향을 조사하여 최대포화축적기간을 제시하고자 실시하였다. Se-SMC 급여기간에 따른 비육후기 한우의 건물섭취량은 급여기간과 셀레늄수준에 의하여 처리구 간에 유의한 차이가 나타나지 않았고, 셀레늄이 사료섭취의 제한요인으로 나타나지 않았다. 또한, 사양시험 개시체중과 종료체중은 급여기간이 증가함에 따라 유의하게 낮았다(p<0.01). 하지만, 총 증체량은 급여기간이 증가함에 따라 유의하게 증가하였으나(P<0.0001), Se-SMC에 의한 효과 또한 나타나지 않았다. 한편, 사료내 셀레늄수준은 한우의 혈중 셀레늄농도를 유의하게 증가시켰으나(p<0.0001), 급여기간에 따른 효과는 나타나지 않았다. 등심내 셀레늄함량은 급여기간과 사료내 셀레늄수준에 의하여 유의한 효과가 나타나지 않았다. 후지내 셀레늄함량은 급여기간이 증가함에 따라 유의하게 증가하였으나(p<0.05), Se-SMC의 4개월 급여군과 6개월 급여군간에는 유의한 차이가 나타나지 않았다. 간내 셀레늄함량은 급여기간의 효과는 나타나지 않았지만, 셀레늄효과는 대조구에 비하여 유의하게 증가하였다(p<0.0001). 따라서, 본 연구에서 근육내 셀레늄에 대한 Se-SMC급여기간은 4개월 정도가 적당할 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권12호
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• pp.1695-1699
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• 2004

The antioxidant enzymes, lipid peroxidation and free radicals assessment were made of the effects of selenium, copper and magnesium on bovine endemic fluorosis under high fluoride, low selenium and low copper productive conditions. Thirty-two beef cattle were selected from high fluoride area, and randomly divided into four groups with eight cattle each as follows: (1) high fluoride control group (HFC); (2) supplemented group with 0.25 mg/kg selenium (HFSe); (3) supplemented group with 15 mg/kg copper (HFCu) and (4) supplemented group with 0.25 mg/kg selenium+15 mg/kg copper+1 mg/kg magnesium (HFSeCuMg) per day for 83 days. Moreover, eight beef cattle were selected from non-high fluoride area as normal control group. Blood samples were collected from cattle on 0 d, 30 d and 83 d respectively, to analyze the enzyme activities and concentration of GSH-px, CAT, SOD, MDA and free radicals. The results showed that the contents of free radicals and MDA in HFC group were significantly higher, and the whole blood GSH-px, CAT, erythrocyte SOD activities were lower than the normal control group. Free radicals, metabolic imbalance and antioxidant disorder therefore, play an important role in fluorosis. However, GSH-px, CAT and SOD activities in HFSe group and HFSeCuMg group at 30 d and 83 d were markedly higher than the same groups at the 0 d and the HFC group at the same time. Likewise, there was a corresponding reduction in the contents of free radicals and MDA. These findings indicated that supplementation with selenium, copper and magnesium elevated high fluoride bovine antioxidant enzymes, and decreased MDA and free radicals contents. But, the activities of supplementation selenium group did not increase until day 83. These results demonstrated that fluorosis was associated with lower serum Se and Cu levels than in the control, and it was therefore concluded that fluorosis is associated with decreased serum levels of these minerals. Long-term high fluoride intake under productive condition enhances oxidative stress in the blood, thereby disturbing the antioxidant defense of cattle. Increased oxidative stress could be one of the mediating factors in the pathogenesis of toxic manifestations of fluoride. It is benefical for high fluoride cattle supplemented with proper selenium, copper and magnesium to increase fluoride excretion and obtain the protective impact of the activity of oxidative enzymes, and to decrease lipid peroxidation and free radicals contents.

• Journal of Nutrition and Health
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• 제24권6호
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• pp.526-533
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• 1991

식이 셀렌이 납섭취에 따른 흰주의 혈액 및 간장중의 $\delta$-Aminolevulinic Acid Dehydratase(ALAD)활성 저해에 미치는 영향을 알아보기 위하여 체중이 141$\pm$5g이 되는 Sprague-Dawley 종 숫쥐를 식이내 납함량을 0(대조군), 200, 1,000, 2,000, 5,000ppm으로 달리하고, 다시 0.5ppm의 셀렌을 첨가한군과 비첨가군등 9군으로 나누어 4주간 사육한 후 체중증가, 식이효율, 혈중hemoglobin, hematocrit치, 혈액 및 간장중의 ALAD 효소활성, 그리고 뇨증 aminolevulinic acid(ALA)의 배설량을 측정한 결과는 다음과 같다. 1. 식이섭취량은 대조군과 납식이군과 별 차이가 없었고, 식이효율 및 체중증가량은 2,000, 5,000 ppm 납식이군이 유의적으로 낮았으나, 셀렌을 첨가했을 때는 대조군과 차이가 없었다. 2. 장기의 무게는 200, 1,000ppm 납식이군에서 신장의 무게가 대조군에 비해 비대했으나, 셀렌을 첨가했을때는 타실험군과 차이가 없었다. 3. Hemoglobin 함량과 hematocrit치는 납함량이 높을수록 대조군에 비해 감소하였으나, 납함량이 1,000, 2,000ppm군에서는 셀렌을 첨가했을때 비첨가군에 비해 증가되었다. 4. 혈액중 ALAD효소 활성은 납함량이 증가될수록 대조군에 비해 ALAD활성이 현저히 감소되었고 200, 1,000, 2,000ppm 군에서는 셀렌을 첨가했을때 비첨가군에 비해 그 활성이 증가되었다. 간장중의 ALAD효소 활성도 혈액에서와 같은 경향으로 납함량이 즐가될수록 감소되었으나, 혈액에서 보다는 ALAD 활성 감소가 적었으며 셀렌을 첨가한 경우 모든 식이군에서 비첨가군에 비래 ALAD 활성이 증가되었다. 5. 뇨중의 ALA 배설량은 ALAD활성과는 반대로 식이내 납함량이 높을수록 그 배설량이 증가되었고, 셀렌 첨가군에서는 그 배설량이 감소되었다.

• 한국식품위생안전성학회지
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• 제38권3호
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• pp.158-163
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• 2023

본 연구에서는 경기도내 유통되고 있는 심해성 어류 50건을 대상으로 수은, 메틸수은의 오염도 및 셀레늄의 함량을 알아보았다. 금아말감법의 수은분석기, ICP-MS를 사용하여 측정하였으며 각 항목별 평균함량은 수은 0.7647 mg/kg (0.0182-5.3620), 메틸수은 0.0764 mg/kg (0.0096-0.8750), 셀레늄 0.4728 mg/kg(0.1075-3.5100)으로 메틸수은은 50건 모두 기준규격(1.0 mg/kg) 이하로 나타났다. 셀레늄은 수은과 결합하여 수은의 독성을 감소시키는 것으로 알려져 있으며, 본 연구과제에서는 일일평균섭취량이 3.3 ㎍/kg으로 일일권장량보다 낮은 수치였다. 유해물질인 수은과 메틸수은은 JECFA에서 설정한 PTWI의 5.7%, 1.8%로 조사되었다. 따라서 도내에서 유통되는 심해성 어류의 수은, 메틸수은과 같은 위해중금속은 안전한 것으로 나타났지만 지속적 모니터링이 필요하다고 판단된다.

• Journal of Nutrition and Health
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• 제28권7호
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• pp.620-628
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• 1995

This study investigated micro-mineral content in human milk along with nutritional intake of lactating women. Milk samples were collected from 33 healthy lactating women and mineral levels were analyzed. Copper, zinc, manganese and selenium were analyzed. The factors that affect the mineral levels in human milk were also investigated longitudinally from 2-5 days to 12 weeks postpartum. Concentrations of manganese and selenium didn't differ significantly within the lactation period. the levels of copper and zinc declined linearly over the first 12 weeks of lactation. Statistically significant quadratic trends were observed in zinc concentrations. There was no significant correlation between maternal age and mineral concentrations throughout the lactation period. In addition, no significant differences were found between the primipara and the multipara for mineral concentrations in human milk.