• Title/Summary/Keyword: selection media

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Assessment of the Ability of Extracellular Enzyme Production in Hybrid Strains of Lentinula edodes by Chromogenic Reaction-based Plate Assay (발색반응 분석법을 이용한 표고 교배균주의 세포외효소 분비 능력 평가)

  • Kwon, Hyuk-Woo;Kim, Jun-Young;Ko, Han-Gyu;Park, Heung-Soo;Kim, Seong-Hwan
    • The Korean Journal of Mycology
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    • v.39 no.2
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    • pp.99-104
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    • 2011
  • Shiitake breeding requires the procedures of mating of two different parental strains and selection of hybrid strains that have good traits for the mushroom production. In this study, we tested the possibility of the use of chromogenic plate-based assay for extracellular enzyme production in order to assess and find good biochemical properties-possessed hybrid strains that were generated from genetic cross of the monokaryotic strains derived from two different parental strains of Lentinula edodes Sanjo-101ho and Sanjo-108ho. We observed that there was difference in the ability of producing ${\beta}$-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease among the monokaryotic strains. We could also comparatively assess that the ability of the seven extracellular enzymes production in the hybrid strains depended on the mating combination of the monokaryotic strains. Our results demonstrate that the assessment method for extracellular enzyme production using chromogenic plate assay could be usefully applied to the assessment of the hybrid strains derived from the breeding procedure of L. edodes.

Understanding the Selective Attention and Animation Induction Device According to the Visual Capture of Audience (관객의 시각포획현상에 따른 선택적 주의집중과 애니메이션 유도장치의 이해)

  • Lee, Jong-Han
    • Cartoon and Animation Studies
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    • s.41
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    • pp.133-152
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    • 2015
  • Some artists and scientists in physics and animation originating from research on its form of expression thanks to the rapid development of the example in the late 20th century image production technology integrating existing media feature, perform a re-creation and pop culture content has been recognized as a key factor. animation of the modern emphasis is also commercial and artistic activities as show whether the artist can not be excluded that also target audience. The audience does not want only to receive offers simply 'seeing' and 'hearing' in the animation requires a more indirect mental met. the other side, the director should lead the audience to immerse myself in work as intended mystification induce the world. where a conflict occurs between the audience and the director and The director needs to have its troubleshooting point to 'Technology of the communication'. Which is reduced to 'How will tell,' is technology communication technologies that are abbreviated representations of animation director is accessible to the audience and it is a close relationship between the psychological aspect of audience. Because, the audience is reproduced in a limited space, but he called on the board of directors and the same time the screen, the audience located at reception and the director located at provide. It is given. led force is given to the director. for this reason, The director needs to pay attention to the psychological aspect of audience this can be explained based on psychoanalytic theory. In this paper, "How can you lie to the audience and the director is the same line?" put down logic that is the animation audience under the logic that takes place visually capture phenomenon "selective attention" and sub-concept of "goal-directed selection' and 'stimulus-driven capturel' for theory of psychology. also, Induction device to elicit selective attention of the audience accordingly, let's consider whether and how they apply in animation.

A study on films selection of education content development for promoting pre-service early childhood teacher's creativity and personality using delphi technique (델파이 기법에 기반한 예비유아교사의 창의·인성 증진 교육콘텐츠 개발을 위한 영화선정 연구)

  • Eom, Se Jin;Youn, Jeong Jin;Kim, Hyoung Jai;Lee, Hee Seung
    • Journal of Digital Convergence
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    • v.12 no.5
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    • pp.403-413
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    • 2014
  • This study is to select educational films suitable for enhancing creativity and personality in order to develop educational contents aimed at pre-service early childhood teacher. First, three film critics based on sub-elements of ego-identity selected final 59 films. Next, three education experts classified the 59 films into small groups based on components each for creativity and personality. As a result, first, films classified according to pre-service early childhood teacher' sub-elements of ego-identity, educational components for creativity, and educational components for personality were 8 in job, 7 in religion, 6 in politics, 7 in philosophical life style, 8 in friendship, 8 in relationship, 7 in gender role, and 8 in leisure activity. Second, verifying suitability of film critics and education experts using Delphi method in order to select educational films to enhance pre-service early childhood teacher' creativity and personality based on films was effective in increasing validity of the results of this research.

Obesity among Secondary School Girls in Ulsan Area (울산지역 중학교 여학생의 비만실태에 관한 연구)

  • 김혜경
    • Journal of the East Asian Society of Dietary Life
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    • v.6 no.1
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    • pp.33-39
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    • 1996
  • The aim of this study is to estimate the prevalence of obesity and factors with it Ulsan secondary school girls. A cross-sectional study involving a sample of 485 students aged 12 to 15 years was obtained form secondary schools. Obesity was determined using body mass index(BMI=wt/Ht$^2$). The findings revealed that the average BMI was 19.3, and 4.5% of girls were either overweight and obese(BMI$\geq$25). Subjects were grouped into three categories based on the BMI, sibling obesity, self-consciousness of obesity, eating patterns such as eating snack after dinner and prejudice in food selection, and food frequency score were significantly associated nth BMI, while nutrition knowledge score was not associated with BMI. The fact that several social, environmental and health factors are associated with obesity means that a multisectorial intervention program must be considered in order to decrease the problem of overweight among secondary girls. Such program should include dietary intervention to modify food habits, nutrition education through mass media, introducing information on prevention and control obesity in school curriculum, and allocating sufficient time in the school schedule for exercise activities.

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International Comparison of Nuclear Energy Conflict in Europe and Northeast Asia from the Viewpoint of New Social Movement: With an Emphasis on the Risk Communication (신 사회운동의 과점에서 본 유럽과 동북아시아의 핵에너지 갈등의 국제적 비교: 모험 커뮤니케이션을 중심으로)

  • Kim, Seong-Jae
    • Korean journal of communication and information
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    • v.25
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    • pp.7-40
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    • 2004
  • Today, nuclear energy conflict is caused from the dangerous radioactive material. The main party of this conflict are politic and economic systems which deride nuclear energy, and the persons concerned which it oppose and the anti-nuclear environment group. If the nuclear waste is transported from one nation to another nation, multi national anti-nuclear group appears as conflict party. We call this domestic and transnational risk communication new social movement. From the viewpoint of system theory, the new social movement can mean the offensive development of self-reference which withstand the "technicalization of communication" through the "symbolically generalized communication media" like money and power. By comparing Northeast Asia and Europe, the nuclear energy conflict did not show a big difference in the selection of nuclear waste storing site. In the Northeast Asia, when Taiwan exports the nuclear waste to North Korea, the international conflict broke out. In Europe, Germany has a hard experience with the construction-plan for the re-treating plant that produces the plutonium from the dangerous nuclear waste, and with the transnational transport of the nuclear waste. The new social movement aims the global paradigm which is able to guarantee the subtainability of ecological environment. The nuclear conflict in the "world risk society" is solved through the "discourse-alliance" which accomplishes sub-politics by crossing the border of class, nation and system.

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A Study on Lambertian Color Segmentation and Canny Edge Detection Algorithms for Automatic Display Detection in CamCom (저속 카메라 통신용 자동 디스플레이 검출을 위한 Lambertian 색상 분할 및 Canny Edge Detection 알고리즘 연구)

  • Han, Jungdo;Said, Ngumanov;Vadim, Li;Cha, Jaesang
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.11 no.5
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    • pp.615-622
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    • 2018
  • Recent advancements in camera communication (CamCom) technology using visible light exploited to use display as an luminance source to modulate the data for visible light data communication. The existing display-CamCom techniques uses the selected region of interest based camera capturing approach to detect and decode the 2D color coded data on display screen. This is not effective way to do communicate when the user on mobility. This paper propose the automatic display detection using Lambertian color segmentation combined with canny edge detection algorithms for CamCom in order to avoid manual region of interest selection to establish communication link between display and camera. The automatic display detection methods fails using conventional edge detection algorithms when content changes dynamically in displays. In order to solve this problem lambertian color segmentation combined with canny edge detection algorithms are proposed to detect display automatically. This research analysed different algorithms on display edge recognition and measured the performance on rendering dynamically changing content with color code on display. The display detection rate is achieved around 96% using this proposed solutions.

A Research of LEACH Protocol improved Mobility and Connectivity on WSN using Feature of AOMDV and Vibration Sensor (AOMDV의 특성과 진동 센서를 적용한 이동성과 연결성이 개선된 WSN용 LEACH 프로토콜 연구)

  • Lee, Yang-Min;Won, Joon-We;Cha, Mi-Yang;Lee, Jae-Kee
    • The KIPS Transactions:PartC
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    • v.18C no.3
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    • pp.167-178
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    • 2011
  • As the growth of ubiquitous services, various types of ad hoc networks have emerged. In particular, wireless sensor networks (WSN) and mobile ad hoc networks (MANET) are widely known ad hoc networks, but there are also other kinds of wireless ad hoc networks in which the characteristics of the aforementioned two network types are mixed together. This paper proposes a variant of the Low Energy Adaptive Cluster Hierarchy (LEACH) routing protocol modified to be suitable in such a combined network environment. That is, the proposed routing protocol provides node detection and route discovery/maintenance in a network with a large number of mobile sensor nodes, while preserving node mobility, network connectivity, and energy efficiency. The proposed routing protocol is implemented with a multi-hop multi-path algorithm, a topology reconfiguration technique using node movement estimation and vibration sensors, and an efficient path selection and data transmission technique for a great many moving nodes. In the experiments, the performance of the proposed protocol is demonstrated by comparing it to the conventional LEACH protocol.

Selection of Functional Lentinula edodes Strains with High Ergothioneine Content using Mono-mono Hybridization (단핵균주간 교잡에 의한 에르고티오네인 함량이 높은 기능성 표고 균주 선발)

  • Kim, Min-Jun;Jeong, Yeun Sug;Jang, Yeongseon;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.507-514
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    • 2021
  • Lentinula edodes have been cultivated extensively not only in South Korea but also in other Asian countries. In terms of taste and nutrition, it is a valuable mushroom that is comparable to other mushrooms. L. edodes contains various physiologically active compounds that promote human health. One compound, ergothioneine, exerts a powerful antioxidant function that inhibits cellular senescence. As it is not biosynthesized by plants or animals, L. edodes is very important for ergothioneine supply. The L. edodes cultivars Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420) of the National Institute of Forest Science (NIFoS) had higher ergothioneine content than the other cultivars, and 11 strains were obtained using mono-mono hybridization between them. The strains were inoculated into sawdust media to obtain fruiting bodies, and the ergothioneine content of each hybrid strain was confirmed using high-performance liquid chromatography (HPLC) analysis. Among the 11 strains, NIFoS 5108 had a higher ergothioneine content than the parental strains, Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420). This study revealed that it is possible to develop cultivars with improved specific functions using mono-mono hybridization.

Development and Usability of a Cognitive Rehabilitation System Based on a Tangible Object for the Elderly (고령자를 위한 실감객체기반 인지재활 시스템의 개발과 사용성 연구)

  • Park, Sangmi;Won, Kyung-A;Shin, Yun-Chan;Park, Ji-Hyuk
    • Therapeutic Science for Rehabilitation
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    • v.8 no.1
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    • pp.51-62
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    • 2019
  • Objective: To develop and verify the usability of a cognitive rehabilitation system with diverse cognitive functional levels based on tangible objects for the elderly population. Methods: A study was conducted to investigate the system's strengths and weaknesses by upgrading it with responses from two groups of 15 patients and 4 occupational therapists. After undergoing three forms of training - regarding executive function, memory, and concentration for a total of 20-30 min, the participants were asked to answer a structured questionnaire about contents of the three forms of training, hardware including the tablet PC functioning as a CPU and display media and tangible objects, and satisfaction of experiential usage of the system. Results: Both groups responded that the most interesting training area was executive function while the least interesting was concentration. Six participants reported that the size of the screen of the tablet PC was inappropriate, and five responded that the size of the tool was inappropriate. All therapists and 40% of the patients responded that they were satisfied with this system. Conclusion: This system's features include easy manipulation of tangible tools for performing training tasks, easy selection of and training in cognitive areas based on users' needs, and automatic adjustment of difficulty level based on users' performance. The training environment was designed to be similar to the natural environment by using tangible objects in both hands as input devices for the system, and the system was considered as an alternative to the lack of community cognitive rehabilitation specialists.

A Review on Measurement Techniques and Constitutive Models of Suction in Unsaturated Bentonite Buffer (불포화 벤토나이트 완충재의 수분흡입력 측정기술 및 구성모델 고찰)

  • Lee, Jae Owan;Yoon, Seok;Kim, Geon Young
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.17 no.3
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    • pp.329-338
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    • 2019
  • Suction of unsaturated bentonite buffers is a very important input parameter for hydro-mechanical performance assessment and design of an engineered barrier system. This study analyzed suction measurement techniques and constitutive models of unsaturated porous media reported in the literature, and suggested suction measurement techniques and constitutive models suitable for bentonite buffer in an HLW repository. The literature review showed the suction of bentonite buffer to be much higher than that of soil, as measured by total suction including matric suction and osmotic suction. The measurement methods (RH-Cell, RH-Cell/Sensor) using a relative humidity sensor were suitable for suction measurement of the bentonite buffer; the RH-Cell /Sensor method was more preferred in consideration of the temperature change due to radioactive decay heat and measurement time. Various water retention models of bentonite buffers have been proposed through experiments, but the van Genuchten model is mainly used as a constitutive model of hydro-mechanical performance assessment of unsaturated buffers. The water characteristic curve of bentonite buffers showed different tendencies according to bentonite type, dry density, temperature, salinity, sample state and hysteresis. Selection of water retention models and determination of model input parameters should consider the effects of these controlling factors so as to improve overall reliability.