• Asian Pacific Journal of Cancer Prevention
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• 제16권11호
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• pp.4671-4675
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• 2015

Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the $IC_{50}$ values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and $751.9{\mu}g/mL$, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

• 식물조직배양학회지
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• 제24권4호
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• pp.197-201
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• 1997

Plant seed oil-bodies or oleosomes ate the repository of the neutral lipid stored in seeds. These organelles in many oilseeds may comprise half of the total cellular volume. Oleosomes are surrounded by a half-unit membrane of phospholipid into which are embedded proteins called oleosins. Oleosins are present at high density on the oil-body surface and after storage proteins comprise the most abundant proteins in oilseeds. Oleosins are specifically targeted and anchored to oil-bodies after co-translation on the ER. It has been shown that the amino-acid sequences responsible for this unique targeting reside primarily in the central hydrophobic tore of the oleosin polypeptide. In addition, a signal-like sequence is found near the junction of the hydrophobic domain and ann N-terminal hydrophilic / amphipathic domain. This "signal" which is uncleaved is also essential for correct targeting. Oil-bodies and their associated oleosins may be recovered by floatation centrifugation of aqueous seed extracts. This simple partitioning step results in a dramatic enrichment for oleosins in the oil-body fraction. In the light of these properties, we reasoned that it would be feasible to create fusion proteins on oil-bodies comprising oleosins and an additional valuable protein of pharmaceutical or industrial interest. It was further postulated that if these proteins were displayed on the outer surface of oil-bodies, it would be possible to release them from the purified oil-bodies using chemical or proteolytic cleavage. This could result in a simple means of recovering high-value protein from seeds at a significant (i.e. commercial) scale. This procedure has been successfully reduced to practice for a wide variety of proteins of therapeutic, industrial and food no. The utillity of the method will be discussed using a blood anticoagulant, hirudin, and industrial enzymes as key examples.

• 한국작물학회지
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• 제39권4호
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• pp.382-388
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• 1994

저장기간과 용도에 따른 대두종실의 건물 및 화학성분의 변화를 비교하기 위하여 장려 3품종에 대하여 2년의 저장기간 차이를 두었으며 용도에 따라서는 장려 5품종과 재래 2품종을 공시재료로 하여 종실을 종피와 자엽(유근포함)으로 분리하여 건물중, 발아율, 단백질, 지방, 전분, 당 및 oligo당을 분석하였다. 1. 3년 저장된 종자에서 건물중의 감소가 나타났으며, 발아율도 급격히 저하되었다. 2. 저장기간동안 자엽의 무게가 현저히 감소하였고, 종피의 구성비율은 증가하였다. 3. 3년 저장된 종자의 종피에서는 단백질, 지방, 탄수화물이 모두 감소하였고, 자엽에서는 단백질의 함량이 41.65%로 현저하게 감소하였다. 4. 종피의 구성비율은 대립종보다 소립종에서 높았다. 5. 밥밑콩에서 자엽의 단백질과 지방함량은 평균 43.59%와 18.25%로 장려품종에 비해 낮았고, 전분과 당의 함량은 평균 5.70%와 11.58%로 현저하게 높았다. 6. Sucrose, raffinose 및 stachyose의 함량은 밥밑콩에서 높았다.

• Journal of Forest and Environmental Science
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• 제27권3호
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• pp.151-156
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• 2011

Seeds of Xanthoceras sorbifolia Bunge were collected from two plantations and two superior trees in Inner Mongolia: and one plantation and one superior tree in Liaoning, China in late August, 2011. Yellowhorn or goldenhorn is an important tree species, from the aspects of source of edible oil and biodiesel and pioneering capacity of degraded and desert land. Characteristics investigated were seed length, width, and weight: weight and volume of 1,000 seeds: and weight and volume of one-liter seeds. The seeds of Qingsonglingxiang No. 1, growing alone in an open space, showed the highest values in seed length (16.08 mm), width (14.48 mm) and weight (1.40 g), while those of Tree No. 160 in Ar Khorqin Banner were the lowest ones: that is, 11.48mm for length, 11.81 mm for width, and 0.73 g for weight, respectively. Traits of seeds varied quite much between trees and among areas; for example, Tree No. 38 and No. 160 produced quite different seeds in several traits, although they are adjacent to each other in the same farm. Weight of 1,000 seeds varied from 718.0 g to 1,010.1 g and volume from 0.76 L to 1.52 L. Weight of one-liter seeds were 522.3 g to 688.2 g, while the number of seeds were 603 to 935. Seeds which were soaked in the water at $4^{\circ}C$ for 2 days showed the highest germination rate (89%) in a 30-day test, which was about 10% to 40% higher than those of non-treatment and dipping treatment at $36^{\circ}C$ followed by keeping under room temperature for 2 days. 81% of seeds in the wet sand at room temperature germinated, while 23% of seeds deprived of seed coat germinated. It is necessary to understand seed traits to select superior clones or provenances for the increased, unfluctuating production of seed.

• 한국식품과학회지
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• 제30권2호
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• pp.260-265
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• 1998

참깨를 170, 200, $230^{\circ}C$로 열풍순환식과 전기팬에서 볶아서 참유한 기름의 향기 성분을 GC와 GC-MS로 분석하여 동정 확인하고 관능 검사를 실시하였다. 총 33개의 향기성분이 확인 되어졌으며 pyrazine류 14종, thiazole류 7종, pyridine류 4종, oxazole류 2종 및 기타 6종 이였다. 전기팬에서 170, 200, $230^{\circ}C$에서 처리된 시료의 총 향기성분의 함량은 각각 145.29, 334.65, 313.04 ppm 이였고 열풍순환식에서는 각각 28.86, 81.20, 313.28 ppm이 였다. 그리고 개별향기 성분중에서는 pyrazine류의 함량비가 가장 높았다. 6개의 시료에 대한 관능 검사의 결과는 $200^{\circ}C$에서 배전식 방법으로 볶은 참깨로부터 제조된 참기름이 가장 바람직한 향기를 가진것으로 판단되어졌다. 이런 현상은 GC 분석의 결과와 일치하였다.

• 한국작물학회지
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• 제51권spc1호
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• pp.304-309
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• 2006

Near infrared reflectance spectroscopy (NIRS) was used to develop a rapid and nondestructive method for the determination of fatty acid composition in sesame (Sesamum indicum L.) seed oil. A total of ninety-three samples of intact seeds were scanned in the reflectance mode of a scanning monochromator, and reference values for fatty acid composition were measured by gas-liquid chromatography. Calibration equations were developed using modified partial least square regression with internal cross validation (n=63). The equations obtained had low standard errors of cross-validation and moderate $R^2$ (coefficient of determination in calibration). Prediction of an external validation set (n=30) showed significant correlation between reference values and NIRS estimated values based on the SEP (standard error of prediction), $r^2$ (coefficient of determination in prediction) and the ratio of standard deviation (SD) of reference data to SEP. The models developed in this study had relatively higher values (more than 2.0) of SD/SEP(C) for oleic and linoleic acid, having good correlation between reference and NIRS estimate. The results indicated that NIRS, a nondestructive screening method could be used to rapidly determine fatty acid composition in sesame seeds in the breeding programs for high quality sesame oil.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1224-1229
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• 2009

The main objective of this study was to investigate possible application of citron (Citrus junos Sieb. ex TANAKA) seeds, which are massively produced as by-products during citron tea process, into functional food materials. First of all, citron seeds were germinated and produced citron shoots were examined for their functional properties. When contents of flavonoids in citron seeds and their germinated shoots were compared, naringenin, neohesperitin, and hesperitin were remarkably increased in shoots after germination while naringin and didymin were decreased. Concentrations of limonin and nomilin were decreased by germination otherwise their unidentified derivatives were newly formed. A methanol extract of citron shoot had lower $IC_{50}$ values [0.13 and 0.07 mg/mL for 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ABTS, respectively] than citron seed extract in radical scavenging activities. Addition of 500 mg/mL of citron shoot extract suppressed fat accumulation in 3T3-L1 adipocytes by 36.9%. Oral administration of olive oil along with citron shoot extract (33 mg/kg body weight) to Sprague Dawley rats effectively inhibited absorption of lipid into a body by decreasing blood triglyceride levels from 105.1 to 74.9 mg/dL 2 hr after olive oil administration. According to these results, citron shoot extract as a rich source of flavonoids can be utilized for functional food ingredients with effective antioxidant and anti-adipogenic properties.

• 한국식품과학회지
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• 제49권5호
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• pp.480-485
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• 2017

본 연구는 생리활성이 우수하다고 알려진 향신료 중 샐러리 종자 80% 에탄올 추출물의 항산화능과 산화안정성을 관찰하였다. 샐러리 종자 80% 에탄올 추출물의 처리에 의해 DPPH 라디칼 소거능은 농도 의존적으로 유의적으로 증가하는 것으로 나타났다. 또한 ABTS 양이온 라디칼 소거활성 역시 농도 의존적으로 라디칼 소거활성이 유의적으로 증가하는 것으로 나타났다. 총 페놀 함량은 $8.2{\pm}2.3mol$ 타닌산 당량/g extract로 나타났고, FRAP 환원력은 $195.0{\pm}12.6mol$ 아스코브산 당량/g extract로 나타났다. 수중유적형 유화계에서의 헤드드스페이스 산소 함량은 대조군의 산소 잔존율 보다 샐러리 종자 80% 에탄올 추출물 처리군 산소 잔존율이 유의적으로 높았고, CDA가는 광산화가 진행됨에 따라 대조군에 비해 지방산화시 발생되는 conjugated dienes의 생성량이 유의적으로 감소하였으며, 지방질 하이드로과산화물의 양 역시 샐러리 종자 80% 에탄올 추출물 처리군이 대조군 보다 유의적으로 감소하는 것으로 나타났다. 이러한 샐러리 종자의 항산화 및 수중유적형에서의 산화안정성은 샐러리 종자 80% 에탄올 추출물에 존재하는 향기성분들에 의해 유래된 것으로 사료된다. 이와 같은 결과로 샐러리 종자 에탄올 추출물은 항산화능이 우수하여 산화안정성이 중시되는 식품에 천연항산화제로서 사용할 수 있을 것으로 사료된다.

• Journal of Nutrition and Health
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• 제15권1호
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• pp.30-38
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• 1982

Experiments were carried out in order to compare the natural antioxidants in the acetonitril extracts of raw and baked seeds of Korean sesame and perilla by thin layer and gas chromatography. The sample was dissolved in n-pentane and extracted with acetonitril and the acetonitril extract was separated by thin layer chromatography using silica gel. The spots were detected by spraying with 2, 6 -dichloroquinone -4-chlorimide, phosphomolybdic acid and dimethylamine as chromogenic reagents. Natural antioxidant, such as ${\delta}-tocopherol$ detected in raw and baked sesame and perilla seed oil by TLC and sesamol was detected only in raw and baked sesame seed oil by GC.

• 한국작물학회지
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• 제63권2호
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• pp.158-163
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• 2018

Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.