• Journal of Korean Society of Forest Science
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• v.67 no.1
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• pp.10-16
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• 1984

Containerized Quercus acutissima seedlings were inoculated with vegetative mycelial inoculum of Pisolithus tinctorius and were grown in a green-house. The ectomycorrhizal fungus, P. tinctorius (Pt) increased growth of the container-grown oak seedlings. Of three treatments, Pt inoculation stimulated remarkably primary lateral roots, shoot growth, and root collar diameter and leaf area development, compared with either non-inoculated or natural soil treatment. In addition, Pt-inoculated seedlings produced ectomycorrhizae, by 71.1 percent. However, noninoculated and naturally grown seedlings were free of natural ectomycorrhizal. Ectomycorthizal roots developed more abundantly in the middle portion of the container than in the top or bottom portions.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.77-85
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• 2004

Development of efficient plant regeneration and genetic transformation protocols (using the Particle Inflow micro-projectile Gun and the shoot-tips as target tissue) of Sorghum bicolor (L.) Moench in terms of expression of the reporter gene, $\beta$-glucuronidase(uidA) is reported here. Two Indian cultivars of sorghum were used in the study, viz. M-35-1 and CSV-15. Plant regeneration was achieved from one-week-old seedling shoot-tip explants via multiple-shoot-clumps and also somatic embryos. The multiple-shoot-clumps were produced on MS medium containing BA (0.5, 1.0 or 2.0 mg/$L^{-1}$), with biweekly subculture. Somatic embryos were directly produced on the enlarged dome shaped expansive structures that developed from shoot-tip explants (without any callus formation) when cultured on MS medium supplemented both with BA (0.5, 1.0 or 2.0 mg/$L^{-1}$) and 2,4-D (0.5 mg/$L^{-1}$). Whereas each multiple-shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing 1.0mg/$L^{-1}$ IBA and successfully transplanted to the glasshouse and grown to maturity with a survival rate of 92%. The plant regeneration efficiency of both the genotypes were similar. After the micro-projectile bombardment, expression of uidA gene was determined by scoring blue transformed cell sectors in the bombarded tissue by an in situ enzyme assay. The optimal conditions comprising a helium pressure of 2200 K Pa, the target distance of 11 cm with helium inlet fully opened and the use of osmoticum have been defined to aid our future strategies of genetic engineering in sorghum with genes for tolerance to biotic and abiotic stresses.

• Korean Journal of Agricultural Science
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• v.10 no.2
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• pp.263-268
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• 1983

In a survey of seed-borne mycofloras of Glycine max, 21 species have been isolated. Alternaria tenuis, Aspergillus spp., Cercospora kikuchii, Penicillium spp. and Fusarium spp. were the predominant fungi. C. kikuchii was isolated more often from purple stained seed than from unstained seed, and Fusarium spp., Phomopsis sp., Aspergillus spp. and Penicillium spp. were isolated less frequently in stained than unstained seeds. In inoculation experiments, C. kikuchii, Colletotrichum dematitum, Phomopsis sp. produced mild to severe symptoms on leaves when suspension of their conidia and mycelia were sprayed on to plants. In soil inoculation experiments, Fusarium oxysporum, F. moniliforme and F. semitectum showed pathogenicity causing seed rot and seedling blight.

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.281-291
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• 2010

The rice CHLH gene encodes the $Mg^{2+}$-chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient oschlh mutant is supported by mitochondrial activity. In this study, we investigated the activity of mitochondrial respiration in the illuminated leaves during oschlh seedling development. Growth of mutant plants was enhanced in the presence of 3% sucrose, which may be used by mitochondria to meet cellular energy requirements. ATP content in these mutants was, however, significantly lowered in light conditions. Low cytosolic levels of NADH in illuminated oschlh mutant leaves further indicated the inhibition of mitochondrial metabolism. This down-regulation was particularly evident for oxidative stressresponsive genes in the mutant under light conditions. Hydrogen peroxide levels were higher in oschlh mutant leaves than in wild-type leaves; this increase was largely caused by the impairment of the expression of the antioxidant genes, such as OsAPXl, OsRACl, and OsAOXc in knockout plants. Moreover, treatment of mesophyll protoplasts with ascorbic acid or catalase recovered ATP content in the mutants. Taken together, these results suggest that the light-mediated inhibition of mitochondrial activity leads to stunted growth of CHLH rice seedlings.

• Korean Journal of Medicinal Crop Science
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• v.22 no.2
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• pp.154-159
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• 2014

This study was carried out in order to investigate the early growth and root development characteristics of Atractylodes japonica container seedling. Experiment was performed by light intensity (100%, 60%, 25% and 10% of full sunlight). Height was surveyed well under 25% of full sunlight (overall 11.013.0%). Root collar diameter was the highest in the full sunlight (7.52 mm). It was the highest fresh weight (1.52 g, 0.92 g, 0.90 g and 3.34 g) under 25% of full sunlight, root dry weight was getting higher in the higher light level and the highest in the full sunlight (0.13 g). Total root length, root project area, root surface area and root volume were the highest under 25% of full sunlight (434.7 cm, $17.4cm^2$, $54.7cm^2$, $0.55cm^3$, respectively) and the lowest under 10% of full sunlight. As a result of surveying the whole experiment, A. japonica is judged nicely by maintaining 25% of full sunlight, excepting 10% of full sunlight. And light levels during the early growth are overly important to improve early growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.287-294
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• 2006

Germination and early elongation of rice after germination were investigated in anoxic air treatment, nitric oxide gas treatment, and six concentrations of mercuric chloride solutions to determine the effects of limited oxygen environment, nitric oxide, and inhibited water flux through cell membrane in $17^{\circ}C$. Anoxic air treatment affected germination of tested six varieties very little. However root elongation rates were severely inhibited while shoot growth was affected less. Reductions in shoot and root elongations demonstrated genotypic variations. Nitric oxide delayed the germination of rice even though it didn't affect the final percent germination. Elongations of root and shoot were inhibited in nitric oxide treatment. The inhibitor effect of nitric oxide on the shoot elongation of rice was less severe, while nitric oxide completely inhibited the root emergence of rice. Concentrations of $HgCl_2$ greater than $300{\mu}M$ dramatically reduced the rate and percentage of germination when compared to distilled water treatment. The reduced percent germination showed the greatest variation among rice varieties in $500{\mu}M$ solution of mercuric chloride. Ansanbyeo, Jinheung, and Odaebyeo were affected less by $HgCl_2$, Nonganbyeo and Sangmibyeo were intermediate, and the germination of Andabyeo was greatly reduced by $HgCl_2$. Root elongation of germinated rice seedlings was more sensitive to oxygen deficits, nitric oxide, and $HgCl_2$ treatments than germination and shoot elongation. In conclusion, poor seedling establishment of rice sown in flooded paddy soils, in which the oxygen supply to the seeds is restricted, appears to the result of limited root elongation rate.

• Journal of Aquaculture
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• v.12 no.1
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• pp.39-45
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• 1999

In order to obtain the basic biological data for artificial seedling production of sea cucumber, Stichopus japonicus, the influence of water temperature and air dry for spawning induction, egg development and larvae rearing was investigated during the period from April, 1995 through September, 1995. Spawning induction rate by the water temperature was 6.0~17.5% and air dry was responsed 1.4~4.0%. Number of eggs spawned of Stichopus japonicus were $50~500\times10^4$ individuals, the fertilization and hatching rate were ranged 84.0~96.0%, 71.4~84.6% respectively. The fertilized egg of Stichopus japonicus appeared mean diameter of $154{\mu}m$. At a constant water temperature of $23^{circ)C$, it become 4 tell stage from fertilization after 2 hours 10 minutes, hatching larvae after 14 hours half, auricularia larvae after 3 days, doliolaria larvare after 11 days and pentactula larvae after 15 days ready for settlement. The suitable food in the larvae reared for 17 days after fertilization were shown the best growth and survival in the larvae food of Chaetoceros calcitrans. Optimum density for larvae rearing were maintained of the larval density lower than 2 individuals/ml.

• Journal of Bio-Environment Control
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• v.6 no.1
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• pp.34-42
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• 1997

Effects of DIF on stem elongation in plug seedlings of mini tomato ‘Mini Carol’ and on the growth and flowering after transplanting were investigated. Stem elongation of seedlings was mainly influenced by day temperature rather than night temperature. When the effect of ＋DIF was compared to that of -DIF under the same average daily temperature(ADT), day temperature had greater impact on internode elongation than night temperature. On the other hand, leaf unfolding rate increased and compactness decreased as ADT in creased. Differences in internode length affected by DIF during seedlings stage DIF progressively diminished during growth period after transplanting. Node position at which first flower was initiated was lowered as ADT decreased. It was concluded that DIF treatment was an applicable tool for control of stem elongation, particularly to reduce stem length without a noticeably adverse effect on the growth and development of plant after transplanting.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.3
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• pp.607-612
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• 2009

For the development of ecological sound proof wall, successful seed germination and growth of plants are very important. In our present study, we observed germination of thirty five different seed species in growth chamber at $24^{\circ}C$, humidity 60% and alternation of 12 hours photoperiods. Germination of same seeds were carried out at room temperature on 6:4, 7:3, 8:2, and 9:1 proportion of pure and mineral soils respectively. Out of studied species, maximum germination was recorded for Perennial penant (92%), followed by Brassica napus (86%), and Silene armeria (82%). The measurement of root/shoot length showed, maximum root/shoot length for Perennial penant, followed by perennial sonata and Rebolsentri sp. Based on germination percentage and root/shoot length of germinating seeds, we considered Perennial penant, perennial sonata and Rebolsentri sp. were proper species for the construction of ecological sound proof walls

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1045-1059
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• 2021

Various abiotic stressors like drought, salinity, temperature, and heavy metals are major environmental stresses that affect agricultural productivity and crop yields all over the world. Continuous changes in climatic conditions put selective pressure on the microbial ecosystem to produce exopolysaccharides. Apart from soil aggregation, exopolysaccharide (EPS) production also helps in increasing water permeability, nutrient uptake by roots, soil stability, soil fertility, plant biomass, chlorophyll content, root and shoot length, and surface area of leaves while also helping maintain metabolic and physiological activities during drought stress. EPS-producing microbes can impart salt tolerance to plants by binding to sodium ions in the soil and preventing these ions from reaching the stem, thereby decreasing sodium absorption from the soil and increasing nutrient uptake by the roots. Biofilm formation in high-salinity soils increases cell viability, enhances soil fertility, and promotes plant growth and development. The third environmental stressor is presence of heavy metals in the soil due to improper industrial waste disposal practices that are toxic for plants. EPS production by soil bacteria can result in the biomineralization of metal ions, thereby imparting metal stress tolerance to plants. Finally, high temperatures can also affect agricultural productivity by decreasing plant metabolism, seedling growth, and seed germination. The present review discusses the role of exopolysaccharide-producing plant growth-promoting bacteria in modulating plant growth and development in plants and alleviating extreme abiotic stress condition. The review suggests exploring the potential of EPS-producing bacteria for multiple abiotic stress management strategies.