• Title/Summary/Keyword: seed storage method

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11S and 7S Globulin Fractions in Soybean Seed and Soycurd Characteristics (콩 종실 단백질 분획(7S, 11S)과 두부특성)

  • Kim, Yong-Ho;Kim, Seok-Dong;Hong, Eun-Hi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.348-352
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    • 1994
  • Soybean seed consists of two major storage protein, the 7S and 11S globulins. For improving the quality of soybean seed protein, an increase of 11S/7S ratio would be a desirable objective because the 11S globulin contains much more the sulfur-containing amino acids than the 7S globulin. In this study, some soybean varieties were used to investigate the analyzing method for 7S and 11S globulins. 7S and 11S globulins couble be fractionated by their different solubilities in tris buffers. Adjusting the pH and tris concentration were major factors affecting the precipitation of the two globulins. And it was possible to screen the soybean genotypes having aberrant subunit compositions of the two globulins by an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soybean proteins. The ratio of 11S to 7S globulin ranged from 1.29 to 1.38. This paper also dealed with the contribution of protein components in soybean seeds to the physical properties of soycurd. It indicated that the soycurd from crude 11S was remarkably harder than that from crude 7S, and springiness and cohesiveness were slightly higher in soycurd having higher proportion of 11S. So, it may concluded that proportion of protein components in soybean seed can be important factor which controls the suitability for soycurd or other foods.

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Effect of Natural Antimicrobial Agent Dipping and Antimicrobial Packaging Films on the Keeping Quality of Green Chilli Peppers (천연 항균제의 침지와 항균 포장필름이 풋고추의 저장성에 미치는 영향)

  • 정순경;조성환
    • Food Science and Preservation
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    • v.8 no.3
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    • pp.264-268
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    • 2001
  • Two antimicrobial extracts of Rheum palmatum L. and Coptis chinesis France root as well as grapefruit seed extract(GFSE) were applied to dipping treatment for green chilli peppers, which were then packed in low density polyethylene(LDPE) films incorporated with 1% antimicrobial extracts and stored 10$^{\circ}C$. Dipping and packaging treatments suppressed the growth of putrefactive microorganisms and the duly ratio of green chilli peppers. In addition, the loss ratio of ascorbic acid content and their weight during the storage was lower with treated green chilli peppers. Consequently, the combined method of dipping and packaging in antimicrobial agents turned out to be superior to dipping treatment or film-packaging in the view point of decay ratio and the keeping qualities of green chilli peppers.

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Effect of Storage Method on Seed Chemical and Germination Traits in Barley (보리 저장방법에 따른 주요 성분 및 발아력 변화)

  • Son, Young-Gu;Son, Jong-Rok;Kim, Sun-Rim;Song, Jin;Baek, Sung-Bum;Kim, Jung-Gon;Nam, Joong-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.3
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    • pp.201-205
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    • 2002
  • This study was carried out to investigate storability and develop suitable storage method for barley. Rough and milled barley such as Olbori(covered barley), Ssalbori(naked barley) and Chalssalbori (waxy naked barley) were packaged in 3P bag and OPP film bag, respectively, and stored at ambient and low temperature warehouse conditions. The weight loss was higher in milled barley than that of rough barley during barley storage. Acid value, one of the most important factor for grain quality evaluation, was increased from 4.9-9.0 mg KOH/100g to 6.5-19.9mg KOH/100g in milled barley at 16 months after storage. Reducing sugar content was increased while germination and water absorption rates were decreased during barley storage period. In terms of penal taste of boiled barley, rough barley could be stored safely more than one year regardless of storage methods where-ase milled barley stored in ambient temperature ware-house produced nasty flavor at 8 to 10 months after storage.

Effect of Gibberellin Acid on Embryo Development and Germination of Dicentra spectabilis (L.) Lem. Seeds

  • Cho, Ju Sung;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.60-60
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    • 2018
  • This study was carried out to develop the seed propagation method of Dicenta spectabilis (L.) Lem. which is an ornamental plant native to Korea. In the previous studies, it was found that the seeds of D. spectabilis were morphophysiologically dormant (MPD), and high and low temperature of stratification were continuously required for the embryo growth and germination of the seeds. Especially, it was most effective to store for 1 month at $20^{\circ}C$ and then to transfer to $4^{\circ}C$. The treatment of $GA_3$ was carried out to promote embryo development and germination. The seeds were submerged in 100, 200 or $500mg{\cdot}L^{-1}$ $GA_3$ for 72 hours and then stored at various conditions as follow. The temperature conditions disposed of this experiment were 1 month at 10, 15, 20, and $25^{\circ}C$ or 2, 4, 8, and 12 months at $4^{\circ}C$, respectively. As a result, the length of embryo and germination rate of the seeds were the best when stored at $4^{\circ}C$ for 8 months after $500mg{\cdot}L^{-1}$ $GA_3$ treatment. Besides, when the seeds stored at $4^{\circ}C$, significant differences in embryo length and germination rate were shown with $GA_3$ concentration and storage period. It was also proved that high-concentration of $GA_3$ could replace the high temperature and could promote germination. Consequentially, the D. spectabilis seeds were classified into intermediate simple levels among MPD types.

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Fabrication arid Performance Tests of Flywheel Energy Storage System using High Tc Superconducting Bearing (고온초전도체 베어링을 사용하는 에너지 저장용 플라이휠 장치의 제작 및 성능 평가)

  • Lee, Ho-Jin;Kim, Ki-Baik;Koh, Chang-Seop;Lee, Soo-Hoon;Hong, Gye-Won
    • 한국초전도학회:학술대회논문집
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    • v.9
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    • pp.310-314
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    • 1999
  • A prototype of Flywheel Energy Storage System with high Tc superconducting bearings was fabricated and tested to verify its applicability for the energy industry. The moment of inertia of assembled wheel with rotor magnets is about 1.072${\times}$10$^{-1}$ Kg-m$^2$. The wheel was designed to withstand its integrity up to the rotation speed of 20,000 rpm. YBCO bulk superconductors prepared by seed growth method were used as bearing to levitate and stabilize the rotating wheel. High speed rotation of the flywheel without mechanical contact was achieved by using specially designed Halbach type motor. The flywheel system showed very high stability during test operation performed up to the speed of about 10,000rpm. The energy loss measured by free decay test performed between 9,300 rpm and 7,000 rpm was calculated as about 45 W.

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Effect of Harvesting Time and Storage Duration of Viscum album Seeds on in vitro and ex vitro Germination on the Branch of Prunus mume

  • Lee, Hyun Woo;Ghimeray, Amal Kumar;Lee, Bo-Duk;Sharma, Pankaja;Shim, Ie Sung;Park, Cheol Ho
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.256-262
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    • 2014
  • Viscum album var. coloratum (mistletoe) is considered as one of the endangered plant species in Korea. Our objective is to restore its population and multiplication of plant by ex situ method. In this research we explored the maximum germination (in vitro) of freshly harvested and stored seeds of mistletoe collected in different time intervals. Ex vitro germination after artificial inoculation on the branches of Prunus mume in different physiological conditions was also monitored. The research revealed that the lately harvested seeds (Feb. and March 2014) were superior over early harvested seeds (Nov. 2013 and Jan. 2014) of mistletoe due to the higher percentage of germination (above 93%). According to the data, it is also revealed that the survival and germination rate of mistletoe seeds decreased with the increase in storage duration. In ex vitro germination, the fluctuated temperature of a glass house in natural condition enhanced (four fold) the rate of germination on the branches of Prunus mume than the constant temperature condition in the glass house.

Development and Characterization of Anti-gliadin Polyclonal Antibody in Wheat

  • Chang, Suk Joo;Hong, Byung Hee;Seo, Yang Weon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.4
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    • pp.339-344
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    • 1999
  • Immunological method has been applied in biochemical genetic analysis of seed storage proteins. We developed and characterized anti-gliadin polyclonal antibody (AGPab) specific to gliadin fractions whose quality and quantity were known to be associated with wheat end-use quality. Reactions of anti-gliadin polyclonal antibody (AGPab) to gliadin were linearly decreased as AGPab and antigen were diluted. Dot-blot and immunoblot assay showed that produced AGPab specifically reacted to gliadin and mainly $\alpha$-, $\beta$-, and ${\gamma}$-gliadin subunits. Enzyme-linked immuno- sorbent assay (ELISA) was applied for quantifi-cation of gliadins in Korean wheat cultivars and breeding lines by using AGPab. High reactions between AGPab and gliadins were found in wheat cultivars Olmil and Olgeurumil. Significant difference of optical densities for alcohol soluble proteins among crop species was found, as wheat showed the highest value (0.697) followed by rye (0.295), and barley (0.066).

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Fabrication of ZnO Nanorod/polystyrene Nanosphere Hybrid Nanostructures by Hydrothermal Method for Energy Generation Applications (에너지 발생소자응용을 위한 수열합성법기반 ZnO 나노로드/Polystylene 하이브리드 나노구조 제조)

  • Baek, Seong-Ho;Park, Il-Kyu
    • Journal of Powder Materials
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    • v.22 no.6
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    • pp.391-395
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    • 2015
  • We report on the successful fabrication of ZnO nanorod (NR)/polystyrene (PS) nanosphere hybrid nanostructure by combining drop coating and hydrothermal methods. Especially, by adopting an atomic layer deposition method for seed layer formation, very uniform ZnO NR structure is grown on the complicated PS surfaces. By using zinc nitrate hexahydrate $[Zn(NO_3)_2{\cdot}6H_2O]$ and hexamine $[(CH_2)_6N_4]$ as sources for Zn and O in hydrothermal process, hexagonal shaped single crystal ZnO NRs are synthesized without dissolution of PS in hydrothermal solution. X-ray diffraction results show that the ZnO NRs are grown along c-axis with single crystalline structure and there is no trace of impurities or unintentionally formed intermetallic compounds. Photoluminescence spectrum measured at room temperature for the ZnO NRs on flat Si and PS show typical two emission bands, which are corresponding to the band-edge and deep level emissions in ZnO crystal. Based on these structural and optical investigations, we confirm that the ZnO NRs can be grown well even on the complicated PS surface morphology to form the chestnut-shaped hybrid nanostructures for the energy generation and storage applications.

Effect of Conjugated Linoleic Acid on Fatty Acid Composition and Lipid Oxidation of Egg Yolk (난황내 Conjugated Linoleic Acid가 지방산 조성과 지방산화에 미치는 효과)

  • Park, Gu-Boo;Lee, Jeong-Il;Ha, Yeong-Lae;Kang,Seuck-Joong;Jin, Sang-Keun;Joo, Seon-Tea
    • Food Science of Animal Resources
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    • v.18 no.4
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    • pp.339-347
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    • 1998
  • The effects of conjugated linoleic acid (CLA) in egg yolk on fatty acid composition and lipid oxidation during chilled storage (4$^{\circ}C$) were investigated. CLA was synthesized according to the method of alkali isomerization using safflower seed oil (SSO). A total of 250 hens (200 days of age) were fed control diet (commercial formula feed for han) or CLA-supplemented diet (1%, 2.5% and 5% CLA) or 5% SSO supplemented diet for 6 weeks, and eggs were collected for analysis of CLA, fatty acid compositons and lipid oxidation. Eggs from CLA-supplemented diets groups showed significantly (p<0.05) higher CLA content compared to those of control group. The contents of linoleic, palmitic, and myristic acid were increased as well as CLA content by feeding a CLA-supplemented diet. However, the contents of oleic and arachidonic acids in egg yolks were decreased by dietary CLA supplementation. The pH of egg yolk increased by the levels of CLA during storage. The contents of CLA were not significantly (p<0.05) changed during chilled storage for 28 days, whereas TBARS were significantly (p<0.05) increased. It is suggested that lipid oxidation of egg yolk might be affected by the levels of CLA in egg yolk due to changes in fatty acid compositions.

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