• Korean Journal of Medicinal Crop Science
• /
• v.3 no.1
• /
• pp.30-34
• /
• 1995

Theses studies were conducted to clarify the germinating characteristics of seed treated with different temperature, light, and ($Gibberellin(GA_3)$) to produce the homogeneous seedlings Angelicogigas NAKAI. The results obtained are summarized as follows: Rate and speed of germinating at $25^{\circ}C$ were higher than $15^{\circ}C$and $20^{\circ}C$ by 87.3% and 68.9% and also the required day for germination at 25°C was shortest by 14.1 days. Germination rate at the light condition was 90.7% and that at the dark was 65.7%. Germinating ability of seed stored at $4^{\circ}C$and $-10^{\circ}C$ for 165 days as dried states was 92.0% and 91.3%, while that at room temperature was 50.3%, but the longer storage of seed than 165 days decreased the ability. Gibberellin treatment by 10ppm to seed stored at $4^{\circ}C$ and by 6ppm to seed stored at $-10^{\circ}C$ increased the germination rate more by 20.9% and 31.7% than nontreated seed.

• Korean Journal of Medicinal Crop Science
• /
• v.28 no.6
• /
• pp.421-427
• /
• 2020

Morus alba, Anti-obesity, C57BL/6 Mice, Expression, Flavonoid, Gene, Mulberry Background: The seed oil of Zanthoxylum schinifolium S. et Z. (sancho) is a traditional cooking oil that has long been sold at a very high price however, depending on the method of extraction and storage, this oil becomes rancid occurs very quickly. Therefore, this study aimed to find a material that prevents rancidity and improves the storage properties of sancho oil. Methods and Results: Sancho oil was extracted using an extraction press, and acid values were compared with commercially available vegetable oils, sancho oil had a higher acid value than other vegetable oils. A very high acid value was observed in sancho oil stored for 6 months, regardless of temperature, requiring an effective storage method. The high acid value and the decrease in turbidity of sancho oil are dependent on the days of sedimentation. Treatment with sodium bicarbonate by concentration resulted in minimal changes in acid value over time. However, minor differences were detected among the treatment concentrations. Ascorbic acid was added to maximize the effect of sodium bicarbonate, and it was observed that ascorbic acid did not improve the antioxidant effect. The sodium bicarbonate and ascorbic acid mixture resulted in minimal change in acid value at temperature up to 25℃. Conclusions: Sancho oil becomes rancid very quicky and requires efficient storage techniques. Sodium bicarbonate and ascorbic acid have been proven to be useful as safe anti-racidity agents without causing harm to humans.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2005.11a
• /
• pp.337-340
• /
• 2005

Rotating annular disks are widely used in data storage devices such as CDs, DVDs(digital versatile disks), and HDs(hard disks). Higher data transfer rate in data storage disks could not be achieved by polycarbonate disks in the present market. The problem can be solved by applying the fiber-reinforce composite materials to the disks. In this paper, an application of composite materials to rotating disks is proposed to increase the critical speed. Dynamic equation is formulated in order to calculate the natural frequency and critical speed for rotating composite by the Galerkin method. The results show that the radially reinforced disk is more effective in increasing critical speed than the circumferentially reinforced disk.

• Korean Journal of Environmental Agriculture
• /
• v.36 no.4
• /
• pp.256-262
• /
• 2017

BACKGROUND: Seed of perilla (Perilla frutescens var. japonica Hara) is short-lived in conventional storage conditions. For long-term conservation of plant species, cryopreservation is the method currently available. This study was performed to find out reliable methods for a long-term storage of seeds of perilla as a genetic resource. METHODS AND RESULTS: Using seeds of 9 perilla cultivars, the effects of desiccation, aging, and cryopreservation on seed germinability and ascorbate peroxidase activity in the seeds were investigated. Initial germinability of the seeds was various, and dry seeds of all cultivars survived cryopreservation without loss of viability. The highest germination was achieved at 4-5% moisture content, and stimulatory effect of cryogenic temperature on the seed germination was observed in some cultivars. Accelerated aging of perilla seeds led to reduction in germination and ascorbate peroxidase activity, and the susceptibility of seeds to aging was different among the tested cultivars. No significant difference in germination was observed for the aged seeds of control and liquid nitrogen exposed. CONCLUSION: The results of this study suggest that cryopreservation at 4-5% moisture content would be a suitable method for long-term conservation of perilla seeds without detrimental effects on germination.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.81-81
• /
• 2019

Ginseng seeds are one of short-lived seeds species which loose their viability easily in the condition of conventional storage. Cryopreservation using liquid nitrogen (LN) has been recommended as a alternative storage for this kind of germplasm short lived or dessiccation-sensitive. This study was performed to find out whether cryopreservation could affect physiological change such as enzyme activity induced by reactive oxygen species. In this work, the redox ratio of ascorbate and glutathione were examined onto ginseng seedlings before and after LN storage of seeds for 1 day using spectrophotometer method. Reduced ascorbate (ASA) was increased while oxidized ascorbate (DHA) was decreased slightly for both after 1d-LN storage. And for glutathione also, reduced form (GSH) was increased while oxidized form (GSSG) was decreased slightly for both after 1d-LN storage. Consequently total phenol compound and ion leakage after LN storage showed no significant differences. Additionally root growth from the seeds after LN storage was not affected by ultra low temperature. From the above results, we may suggest that cryopreservation could be recommended for storage tool of ginseng seeds even with low viability also and expected to make slower seed aging process during preservation period through further study.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.4
• /
• pp.342-348
• /
• 2004

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the $60\%$ isopropanol extract of soybean(Glycine max [L.] Merr.) seed revealed two abundant proteins with molecular masses of 19 and 10 kDa. Amino acid analysis revealed that the isopropanol-extractable protein fraction was rich in cysteine. Two-dimensional gel electro-phoretic analysis indicated that the 19kDa and 10kDa proteins had pI of 4.2 and 4.0 respectively. Peptide mass fingerprints of trypsin digests of the two proteins obtained using matrix-assisted, laser desorption/ionization-time of flight (MALDI-TOF) mass spectroscopy revealed the 19kDa protein was Kunitz trypsin inhibitor and the 10kDa protein was Bowman-Birk proteinase inhibitor. When resolved under non-denaturing conditions, the isopropanol-extracted proteins inhibited trypsin and chymotrypsin activity. Results presented in this study demonstrate that isopropanol extraction of soybean seed could be used as a simple and rapid method to obtain a protein fraction enriched in Kunitz trypsin and Bowman-Birk proteinase inhibitors. Since proteinase inhibitors are rich in sulfur amino acids and are putative anticarcinogens, this rapid and inexpensive isolation procedure could facilitate efforts in nutrition and cancer research.

• Korean journal of food and cookery science
• /
• v.24 no.6
• /
• pp.811-817
• /
• 2008

The principal objective of this study was to evaluate the effects of baking method on the quality and shelf-life of Maejakgwa containing nuts and seeds. Four different types of nuts and seeds (almond, cashewnut, coconut, and sunflower seed) were added to flour, and baking method was applied to prepare the Maejakgwa, physicochemical properties of which were then evaluated. The proximate composition analysis demonstrated that crude protein, crude fat, and ash contents were higher in the Maejakgwa containing nuts and seeds than in the control group. The lightness (L) value of the control Maejakgwa was lower than that observed in the groups containing nuts and seeds. Maejakgwa made with cashewnut, coconut, and sunflower seed evidenced higher yellowness (b) values than were recorded in the control and coconut groups. The incorporation of cashewnut, coconut and sunflower seed into the Maejakgwa lowered the hardness values (p<0.05). Our consumer test demonstrated that Maejakgwa prepared with almond was the most highly preferred, and the control group was the least favored. After 30 days of storage at $60^{\circ}C$, the peroxide value of the Maejakgwa containing sunflower seed was substantially higher than in any other groups, thereby suggesting that the addition of sunflower seeds resulted in accelerated lipid oxidation. No noticeable changes in peroxide values were observed in the groups prepared with coconut and cashewnut during storage time. The acid value increased with increasing storage time, and the addition of sunflower seed evidenced the highest values as compared to other groups.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.19 no.2
• /
• pp.164-168
• /
• 2014

To determine the optimal storage method and longest possible storage period of Zostera marina seeds, we examined post-storage germination rates using different storage methods and periods for Z. marina seeds harvested in southern coast of Korea. Storage methods included in seawater at room temperature with air supply, seawater at $4^{\circ}C$, seawater at $30^{\circ}C$, an aquarium with continuous seawater circulation, seawater at room temperature in an airtight tank, a refrigerator at $-20^{\circ}C$, and a desiccator at room temperature. Germination rates of Z. marina seeds stored in seawater at $4^{\circ}C$ showed highest germination (52.0%), whereas those of seeds stored in seawater at room temperature and an aquarium were 27.4% and 17.7%, respectively. But the seeds stored in seawater at $30^{\circ}C$, a refrigerator, and a desiccator did not germinate. Storage periods were 10, 20, 30, 60, 180 and 240 days of storage. Z. marina seeds maintained germination rates of 46.4~52.4% until 10~60 days of storage, but showed rapidly decreasing germination rates after then and no germination after 240 days.

• Journal of Platform Technology
• /
• v.6 no.4
• /
• pp.69-77
• /
• 2018

In the case of personal information files containing personal information, there is insufficient awareness of personal information protection in end-point areas such as personal computers, smart terminals, and personal storage devices. In this study, we use Diffie-Hellman method to securely retrieve personal information files generated by web crawler. We designed SEED and ARIA using hybrid slicing to protect against attack on personal information file. The encryption performance of the personal information file collected by the Web crawling method is compared with the encryption decryption rate according to the key generation and the encryption decryption sharing according to the user key level. The simulation was performed on the personal information file delivered to the external agency transmission process. As a result, we compared the performance of existing methods and found that the detection rate is improved by 4.64 times and the information protection rate is improved by 18.3%.

• Horticultural Science & Technology
• /
• v.29 no.4
• /
• pp.352-357
• /
• 2011

Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at $25^{\circ}C$ room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.