• Journal of Wetlands Research
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• v.22 no.2
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• pp.81-91
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• 2020

As part of method for ecologically utilizing abandoned paddy, potential of the abandoned paddy as a target site for ex-situ conservation of 9 endangered species of different life form was confirmed by considering the nature of rice paddy. In order to create Dum-bung, a component of traditional Korean rice paddy, a part of the abandoned paddy was modified to change the water environment. The seeds, asexual reproductive and sexually reproductive individuals of each endangerd species were transplanted into the abandoned paddy to observe the survival rate and phenological response for 1 year, and then monitored for 2 years. As a result, Hydrophyte 4 taxa (Euryale ferox, Saururus chinensis, Dysophylla yatabeana, Menyanthes trifoliata), Geophyte taxa 1 (Epilobium hirsutum), and Hemicryptophyte taxa 1 (Cicuta virosa) could be introduced into the abandoned paddy. In particular, Euryale ferox, Dysophylla yatabeana, and Menyanthes trifoliata should be introduced into Dum-bung, and Saururus chinensis, Epilobium hirsutum, and Cicuta virosa should be introduced into paddy wetland. Growth of Euryale ferox and Brasenia schreberi was inhibited by herbaceous species, and the growth of Epilobium hirsutum was inhibited by herbivores. Therefore, in order to help efficient settlement of endangered plants introduced in abandoned paddy, it is necessary to remove herbs that inhibit growth and to manage herbivores. In addition, it is necessary to prevent the collapse of paddy bank by planting on the paddy field trees or herbaceous forming vegetation mat. When using abandoned paddy ecologically, it is effective to diversify the moisture environment by creating a Dum-bung to increase biodiversity.

• Korean Journal of Agricultural Science
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• v.19 no.1
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• pp.16-27
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• 1992

To determine a potential of new sludge fertilizer for horticultural crops, comparative studies between commercial fertilizers (Jandibiryo and Wonyebokbi) and sludge fertilizer (Sludgebiryo) were made through examining the growth responses on zoysiagrass (Zoysia japponica Steud.) and several horticultural plants. 1. The pH of new sludge fertilizer remained near 6.5 regardless the particle size. The solubility of elements was highest in phosphorus, followed by nitrogen and potassium in the order. Especially, desorption of potassium was continued up to 48 hrs after solubilization. 2. There was an increase in shoot number per plant, length of stolon and rhizome, and root weight as well as clipping yield of zoysiagrass in the treatment of large size Sludgebiryo compared to small one and Jandibiryo. 3. Regardless the size of fertilizers, Sludgebiryo increased flower numbers in salvia (Salvia officinalis L. 'Hatzazz') compared to Wonyebokbi, although the difference was not great, However, leaf area and fresh weight of plant were more increased in Wonyebokbi application. 4. Flower diameter of marigold (Tagetes erecta L. 'Inca') was slightly increased in Sludgebiryo application, but the average number of lateral shoots and fresh weight per plant were significantly increased in the treatment of Wonyebokbi application. 5. Sludgebiryo effectively increased the length of both main and lateral shoots, number of flowers and weight of shoot in vinca (Vinca rosea L. 'Little Linde'), but root growth of plant was higher in Wonyebokbi application. 6, No differences between Wonyebokbi and Sludgebiryo were found in promoting the growth of leaves of perilla (Perilla frutescens Hara 'Yubsil' ), but chlorophyll content and seed weight were slightly higher in the application of Wonyebokbi compared to Sludgebiryo. In conclusion, the effectiveness of Sludgebiryo for horticultural plants was almost equal to commercial fertilizers such as Jandibiryo or Wonyebokbi. Expecially, Sludgebiryo appeared to effective on the growth of zoysiagrass, and the increase of flower size and numbers in flower crops. Results indicate that new-made Sludgebiryo can be recommended for turfgrass culture, and the flower crops in which quality depends on flower number and flower size.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.61-62
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• 2003

Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022$^{\circ}$C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of either germination medium ($\frac{2}{1}$strength Murashige and Skoog (1962) salts with 2.5 g/l activated charcoal) or same medium with copper sulphate adjusted to 0.25 mg/1 to compensate for copper adsorption by activated carbon. 2% and 4% maltose was substituted by 7.5% and 13% PEG respectively to improve the yield of the embryos. Substitution of' maltose with PEG was clearly beneficial to embryo development. When 2% of the maltose was replaced with 7.5% PEG, many embryos developed to large bullet-shaped embryos. At latter stages of development most embryos callused and stopped development. A few short, barrel-shaped cotyledonary embryos formed that were covered by callus on the sides and base. When 4% of the maltose was removed and substituted with 13% PEG, the embryos developed further, emerging from the callus and increasing yield slightly. Microscopic examination of the cultures showed differing morphologies, varying from mostly single cells or clumps to well-formed somatic embryos that resembled early zygotic embryos only liquid cultures with organized early-stag. A procedure for converting and acclimating germinants to growth in soil and greenhouse conditions is also tested. Seedling conversion and growth were highly related to the quality of the germinant at the time of planting. Germinants with larger shoots, longer, straighter hypocotyls and longer roots performed best. When mature zygotic embryos germinate the root emerges, before or coincident with the shoot. In contrast, somatic embryos germinate in reverse sequence, with the cotyledons greening first, then shoot emergence and then, much later, if at all, the appearance of the root. Somatic seedlings, produced from the maturation medium, showed 100% survival when planted in a field setting. Somatic seedlings showed normal yearly growth relative to standard seedlings from natural seed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.3
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• pp.278-286
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• 2019

Sesbania, an annual herb, is known for its high forage value and salt tolerance. It has merits as a forage crop that is adaptable to reclaimed land in the Republic of Korea. Therefore, we collected Sesbania genetic resources from the Republic of Korea and other countries, and conducted experiments to evaluate their potential as a forage crop in Korean climate and soil conditions. In the preliminary experiments, 15 genetic resources which were able to set seeds in Korean environment were selected out of a total of 46 collected genetic resources. Among 15 genetic resources, SL13 was the tallest and it was followed by that of SC04, SR01 and SE07. The accessions with the earliest flowering started flowering 101 days after sowing and set seed in early August. Fifteen accessions were evaluated for their salt tolerance at germination stage based on germination rate and growth of germinated seedlings at 0 mM, 150 mM and 300 mM NaCl concentrations. Five genetic resources like SC04, SL13, SS20, SS24 and SR01 were selected to be tolerant to NaCl treatment. Forage value was evaluated based on crude protein, acid detergent fiber, neutral detergent fiber and in vitro dry matter digestibility. The forage value of leaves was significantly higher than that of stems, and the forage value of the stem was slightly better than that of rice straw. The forage value of leaves of all the genetic resources was higher than grade 1 by the American Forage and Grassland Council grade. Among five selected genetic resources, the relative feed value of SC04 was the highest and it was followed by that of SS20, SL13, SS24 and SR01.

• Journal of Food Hygiene and Safety
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• v.37 no.4
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• pp.225-231
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• 2022

In this study, the conditions of dry heat treatment (21 days at 65℃, 16 days at 70℃, 10 days at 75℃, and 7 days at 80℃) were investigated to inactivate Bacillus cereus ATCC 12480, Listeria monocytogenes ATCC SSA81, Staphylococcus aureus ATCC 6538, Escherichia coli O157:H7 ATCC 43894, and Salmonella Typhimurium ATCC 14028 on alfalfa seeds, without affecting the rate of germination of seeds. Alfalfa seeds were inoculated at levels of 6-7 log CFU/g and treated with dry heat at 65℃, 70℃, 75℃, and 80℃; thereafter, the rate of seed germination was determined. The rate of germination was set at 70%, according to the market standards. The bacteria were inactivated when B. cereus was treated with dry heat for 21 days at 65℃, 18 days at 70℃, 14 days at 75℃, and 4 days at 80℃; L. monocytogenes was treated for 21 days at 65℃, 18 days at 70℃, 12 days at 75℃, and 7 days at 80℃; S. aureus was treated for 18 days at 65℃, 18 days at 70℃, 11 days at 75℃, and 4 days at 80℃; E. coli O157:H7 was treated for 21 days at 65℃, 18 days at 70℃, 12 days at 75℃, and 6 days at 80℃; and Sal. Typhimurium was treated for 24 days at 65℃, 22 days at 70℃, 14 days at 75℃, and 7 days at 80℃. For all bacteria, the D-value (R² = 0.5656-0.7957) significantly decreased when the temperature increased from 65℃ to 80℃ (P<0.05). Since dry heat treatment of alfalfa seeds at 80℃ for 7 days affects their germination rate, dry heat treatment at 75℃ for 14 days is the most effective way to ensure their safety. This study suggests a potential method of bacterial inactivation using dry heat treatment to increase the microbiological safety of sprouts.

• Journal of Life Science
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• v.33 no.10
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• pp.783-790
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• 2023

Modern people have an increased incidence of metabolic diseases due to changed eating habits, and diabetes is considered the most significant metabolic disease. Given that existing diabetes treatments are accompanied by side effects, the aim of this study was to identify traditional natural products that have anti-diabetic activity. The potential anti-diabetic and antioxidant activities of natural products were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, α-glucosidase assay, and protein tyrosine phosphatase 1B (PTP1B) inhibition assay. Methanol extracts of Ulmus davidiana var. japonica, Acer tegmentosum branches, Nelumbo nucifera seeds, and Carthamus tinctorius seeds were found to have high anti-diabetic activity and further fractionated with solvents using ethyl acetate and butanol. Consequently, the ethyl acetate fraction of C. tinctorius seeds (MG-11-E) with high α-glucosidase and PTP1B inhibitory activity was selected. MG-11-E was subjected to preparative thin layer chromatography, and fraction #6 showed high α-glucosidase and PTP1B inhibitory activity. Fraction #6 was analyzed and fractionated via high performance liquid chromatography with 50% methanol as the mobile phase, and anti-diabetic activity was observed in the sample that eluted after 4 min as a single peak. The α-glucosidase inhibitory activity exhibited by this sample seemed to be greater than the PTP1B inhibitory activity; thus, it was concluded that a greater anti-diabetic therapeutic effect may be achieved by combining this agent with natural products that inhibit PTP1B activity.

• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.303-310
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• 2023

The current study was conducted to compare the effect of crossbred on the body weight and laying performance of Korean native chicken from hatch to week 40. A total of 873 one-day-old chicks were divided into twelve crossbreds (i.e., CFCK, CFYC, CFYD, CKCF, CKYC, CKYD, YCYD, YCCF, YCCK, YDCF, YDCK, and YDYC) and commercial layer (Hy-Line Brown) were obtained as a counterpart in the study. All the birds are raised in battery cages (76 × 61 × 46 cm³) and then raised until 14 weeks and subsequently moved to layer battery cages (60 × 25 × 45 cm³) and raised until 16 weeks. The body weight and viability were measured biweekly from hatching to week 16. The week of 16, body weight range was about 1,010.24 to 1,411.77 g. The body weight of specific crossbreeds (i.e., CKCF, YCYD, and YDYC) was found to be comparable to that of Hy-Line Brown). The viability hatch to week 14 range was about 55 to 100% and however week 14 to 16 range was 80 to 100%. The crossbred (i.e., CKCF) recorded superior (P<0.05) viability throughout the whole experiment period, even compared with Hy-Line Brown (100% vs 96%). Our results are indicating that crossbreds Korean native chicken including CKCF, and YDYC has the potential to enhance key features of laying hens during the growing phase like optimal body weight and higher viability.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.4
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• pp.304-312
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• 2023

Plant breeding is a time-consuming process, mainly due to the limited annual generational advancement. A speed breeding system, using LED light sources, has been applied to accelerate generational progression in various crops. However, detailed protocols applicable to soybeans are still insufficient. In this study, we report the optimized protocols for a speed breeding system comprising 12 soybean varieties with various maturity ecotypes. We investigated the effects of two light qualities (RGB ratio), three levels of light intensity (PPFD), and two soil conditions on the flowering time and development of soybeans. Our results showed that an increase in the red wavelength of the light spectrum led to a delay in flowering time. Furthermore, as light intensity increased, flowering time, average internode length, and plant height decreased, while the number of nodes, branches, and pods increased. When compared to agronomic soil, horticultural soil resulted in an increase of more than 50% in the number of nodes, branches, and pods. Consequently, the optimal conditions were determined as follows: a 10-hour short-day photoperiod, an equal RGB ratio (1:1:1), light intensity exceeding 1,300 PPFD, and the use of horticultural soil. Under these conditions, the average flowering time was found to be 27.3±2.48 days, with an average seed yield of 7.9±2.67. Thus, the speed breeding systems reduced the flowering time by more than 40 days, compared to the average flowering time of Korean soybean resources (approximately 70 days). By using a controlled growth chamber that is unaffected by external environmental conditions, up to 6 generations can be achieved per year. The use of LED illumination and streamlined facilities further contributes to cost savings. This study highlights the substantial potential of integrating modern crop breeding techniques, such as digital breeding and genetic editing, with generational shortening systems to accelerate crop improvement.