• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3383-3387
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• 2015

Portulaca oleracea (Family: Portulacaceae), is well known for its anti-inflammatory, antioxidative, anti-bacterial, and anti-tumor activities. However, cytotoxic effects of seed oil of Portulaca oleracea against human liver cancer (HepG2) and human lung cancer (A-549) cell lines have not been studied previously. Therefore, the present study was designed to investigate the cytotoxic effects of Portulaca oleracea seed oil on HepG2 and A-549 cell lines. Both cell lines were exposed to various concentrations of Portulaca oleracea seed oil for 24h. After the exposure, percentage cell viability was studied by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed a concentration-dependent significant reduction in the percentage cell viability and an alteration in the cellular morphology of HepG2 and A-549 cells. The percentage cell viability was recorded as 73%, 63%, and 54% by MTT assay and 76%, 61%, and 50% by NRU assay at 250, 500, and $1000{\mu}g/ml$, respectively in HepG2 cells. Percentage cell viability was recorded as 82%, 72%, and 64% by MTT assay and 83%, 68%, and 56% by NRU assay at 250, 500, and $1000{\mu}g/ml$, respectively in A-549 cells. The 100 $100{\mu}g/ml$ and lower concentrations were found to be non cytotoxic to A-549 cells, whereas decrease of 14% and 12% were recorded by MTT and NRU assay, respectively in HepG2 cells. Both HepG2 and A-549 cell lines exposed to 250, 500, and $1000{\mu}g/ml$ of Portulaca oleracea seed oil lost their normal morphology, cell adhesion capacity, become rounded, and appeared smaller in size. The data from this study showed that exposure to seed oil of Portulaca oleracea resulted in significant cytotoxicity and inhibition of growth of the human liver cancer (HepG2) and human lung cancer (A-549) cell lines.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.6
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• pp.785-789
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• 2003

To accept basic data of utilizing of Comus of officianalis as a raw material of new food and industrial products, major chemical components were investigatied. Comparing proximate composition of flesh and seed of Cornus officianalis, seed contained higher crude ash, crude protein, crude fat and crude fiber, with less moisture and reducing sugar. The main components of free sugars in flesh and seed were fructose. The main components of organic acid in fresh and seed were malic and citric acid. Analysing total amino acids, 18 kinds of components were isolated from Cornus officianalis. The total amino acid contents of flesh and seed were 230.41 mg% and 883.81 mg%, respectively. Although the amino acid compositions of flesh and seed were different, glycine, leucine, histidine and lysine were the major components in both portion. The total amount of free amino acid were less than those of total amino acids. As a results of mineral analysis, the content of K was much higher than those of Fe, Zn and Cu. The contents of linolenic and linoleic acid were higher than those of oleic, palmitic and stearic acid.

• Applied Microscopy
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• v.31 no.2
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• pp.109-116
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• 2001

Safflower has been cultivated in Korea and thought to have excellent effects on bone in oriental medicine and folk remedy and has been taken for a long time. Safflower is thought to be helpful for the development and sustenance of bones according to the result of recent assay of its components. Otherwise, any reliable experimental data have not been suggested so far. We have carried out this study to examine the prophylactic effects of safflower-seed-powder on the prevention of osteoporosis induced by the ovariectomy 12 Weeks-old Sprague-Dawley rats weighing about 230 g was kept in the experimental condition and used in this study. Animals were taken 0.3 g of safflower-seed-powder once a day for 1, 3, 5, and 7 weeks after ovariectomizing both ovaries and observed the fine structure of tibia. Tissues were fixed with traditional SEM preparation methods and decalcified for 10 hours with 10% nitric acid and dehydration, drying, and gold-coating were followed by the routine procedures and observed with scanning electron microscope (Hitachi, S-450). Loss of bone was started just after ovariectomy and thickness of bone from the medullary cavity to the compact bone was reduced and the extension of medullary cavity was serious in the control group of 7 weeks. Experimental groups taken safflower-seed-powder showed similar findings from 1 week to 7 weeks. These results suggest that the safflower-seed-powder is thought to be efficient for the prevention of osteoporosis owing to the deficiency of female sex hormone.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.499-511
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• 2017

This study was done to examine antioxidant compounds, antioxidative activity and chemical properties in organically cultivated sorghum seed and their sprout, and to examine as a basic data for various application methods as an organic farming product of sorghum. The crude protein and mineral contents in the sprout had 2.5 and 1.6~9.87 folds more than sorghum seed, respectively. Total polyphenol content as antioxidant was more in the sprout showing $4,135mg\;GAE\;g^{-1}$ than the seed, whereas total flavonoid content was relatively higher in the seed showing $3.86mg\;GAE\;g^{-1}$. ABTS radical scavenging activity in the seed was observed by higher level as $15.78mg\;TE\;g^{-1}$. However, The sprouts with and without natural light were no significant difference. DPPH radical scavenging activity in the seed exhibited activity of 11 folds or more compared to that of the sprout. The highest activity was detected from the seeds and the sprouts in Donganmae. Although sorghum was no significant correlations among the varieties, it showed correlation with between the light-treated condition and antioxidation.

• Korean Journal of Environment and Ecology
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• v.28 no.6
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• pp.705-714
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• 2014

This text was analyzed and investigated the aerial part plants and buried seed plants at coniferous forest and broadleaved forest in Yesan-gun, in order to offer the basic data of potential natural vegetation change on secondary forest. Plants of buried seed germination were consisted of 29 taxa in coniferous forest (28 species, 1 varieties, of 27 genus, 20 families) and 36 taxa in broadleaved forest (34 species, 2 varieties, of 32 genus, 18 families). Family classification of buried seed plant was the most in Compositae, and emergent plot frequency was the highest of Cyperus amuricus in coniferous forest and Crepidiastrum sonchifolium in broadleaved forest. The soil depth of the most plants appearance was 0~10 cm in coniferous forest and 0~5 cm in broadleaved forest, and the soil depth of the most population appearance was 0~2 cm in coniferous forest and broadleaved forest. Population of buried seed germination was decreased according as soil is deep. Crepidiastrum sonchifolium was a plant that population of buried seed germination is the most. Similarity index of the aerial part plants and buried seed plants was low as 0.22, and coniferous forest and broadleaved forest was 0.40.

• Journal of the Korean Society for Nondestructive Testing
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• v.36 no.1
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• pp.35-44
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• 2016

Seed viability is one of the most important parameters that is directly related with seed germination performance and seedling emergence. In this study, a hyperspectral imaging (HSI) system having a range of 1000-2500 nm was used to classify viable watermelon seeds from nonviable seeds. In order to obtain nonviable watermelon seeds, a total of 96 seeds were artificially aged by immersing the seeds in hot water ($25^{\circ}C$) for 15 days. Further, hyperspectral images for 192 seeds (96 normal and 96 aged) were acquired using the developed HSI system. A germination test was performed for all the 192 seeds in order to confirm their viability. Spectral data from the hyperspectral images of the seeds were extracted by selecting pixels from the region of interest. Each seed spectrum was averaged and preprocessed to develop a classification model of partial least square discriminant analysis (PLS-DA). The developed PLS-DA model showed a classification accuracy of 94.7% for the calibration set, and 84.2% for the validation set. The results demonstrate that the proposed technique can classify viable and nonviable watermelon seeds with a reasonable accuracy, and can be further converted into an online sorting system for rapid and nondestructive classification of watermelon seeds with regard to viability.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.145-156
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• 2023

In this study, Fourier-transform near-infrared (FT-NIR) spectra of Korean red pine seeds stored at -18℃ and 4℃ for 18 years were analyzed. To develop seed-germination prediction models, the performance of seven machine learning methods, namely XGBoost, Boosted Tree, Bootstrap Forest, Neural Networks, Decision Tree, Support Vector Machine, PLS-DA, were compared. The predictive performance, assessed by accuracy, misclassification, and area under the curve (0.9722, 0.0278, and 0.9735 for XGBoost, and 0.9653, 0.0347, and 0.9647 for Boosted Tree), was better for the XGBoost and decision tree models when compared with other models. The 54 wave-number variables of the two models were of high relative importance in seed-germination prediction and were grouped into six spectral ranges (811~1,088 nm, 1,137~1,273 nm, 1,336~1,453 nm, 1,666~1,671 nm, 1,879~2,045 nm, and 2,058~2,409 nm) for aromatic amino acids, cellulose, lignin, starch, fatty acids, and moisture, respectively. Use of the NIR spectral data and two machine learning models developed in this study gave >96% accuracy for the prediction of pine-seed germination after long-term storage, indicating this approach could be useful for non-destructive viability testing of stored seed genetic resources.

• Journal of Wetlands Research
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• v.25 no.4
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• pp.237-247
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• 2023

Paddy fields not only provide a variety of ecosystem services but also serve as crucial habitats for biodiversity conservation. Recently, their ecological value and significance have been increasingly emphasized. Therefore, this study aimed to investigate the characteristics of soil seed banks and analyze their correlation with soil environmental factors in Conventional Paddy field (CP) and Organic Paddy field (OP) with different farming practices. Our results revealed that the vegetation in CP was simple, resulting in low plant diversity in the soil seed banks. On the other hand, the vegetation in OP was relatively diverse, leading to higher plant diversity in the soil seed banks. Additionally, distinct differences in soil environmental characteristics were observed between OP (K, Ca, Mg, Na, Fe, Mn, B↓) and CP (K, Ca, Mg, Na, Fe, Mn, B↑). These results suggest that variations in agricultural practices for rice cultivation have influenced the structure and diversity of vegetation and soil seed banks. Furthermore, these agricultural practices have had both direct and indirect effects on soil environmental factors. Our findings can serve as fundamental data for evaluating biodiversity conservation in agricultural ecosystems, ecosystem restoration, and ecological value assessment.

• Corrosion Science and Technology
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• v.4 no.6
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• pp.231-235
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• 2005

The inhibitive effect of extracts of tamarind seeds and jackfruit seeds, curry leaves and henna leaves on corrosion of mild steel in 1M HCl solution have been studied by weight loss, potentiodynamic polarization and impedance measurements. Results obtained from the electrochemical techniques were in good agreement with weight loss results. From the weight loss data, the values of surface coverage ($\Theta$) and corrosion rate were calculated. The inhibition efficiency (IE) increased with increasing inhibitor concentration in 1M HCl solution. In all the cases the adsorption of the natural product extracts on the mild steel surface from 1M HCl follows the Langmuir adsorption isotherm relationship. Potentiodynamic polarization studies reveal the fact that all the four natural product extracts act as mixed type inhibitors. The decrease in the inhibition efficiency follows the order: Extracts of jackfruit seed>henna leaves>curry leaves>tamarind seed.

• Convergence Security Journal
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• v.5 no.3
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• pp.87-92
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• 2005

The performance of a cryptographic module is the most important thing to achieve a high performance VPN system which realizes information security by encrypting and decrypting all the communicating data packets. However the cryptographic operations require much computation power and software cryptographic systems reveal bad performance. Thus, it is strongly recommended to develop a VPN system employing hardware component. This paper introduces a case study of developing a PCI add-on card which supports several block cipher algorithms such as DES, 3DES, AES, and SEED. The performance of them was measured by embedding the card in a commercial VPN system.