• Molecules and Cells
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• v.39 no.5
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• pp.375-381
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• 2016

MicroRNAs (miRNAs) are small non-coding RNAs (~22 nucleotides) regulating gene expression at the post-transcriptional level. By directing the RNA-induced silencing complex (RISC) to bind specific target mRNAs, miRNA can repress target genes and affect various biological phenotypes. Functional miRNA target recognition is known to majorly attribute specificity to consecutive pairing with seed region (position 2-8) of miRNA. Recent advances in a transcriptome-wide method of mapping miRNA binding sites (Ago HITS-CLIP) elucidated that a large portion of miRNA-target interactions in vivo are mediated not only through the canonical "seed sites" but also via non-canonical sites (~15-80%), setting the stage to expand and determine their properties. Here we focus on recent findings from transcriptome-wide non-canonical miRNA-target interactions, specifically regarding "nucleation bulges" and "seed-like motifs". We also discuss insights from Ago HITS-CLIP data alongside structural and biochemical studies, which highlight putative mechanisms of miRNA target recognition, and the biological significance of these non-canonical sites mediating marginal repression.

• Journal of Biomedical Engineering Research
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• v.31 no.6
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• pp.427-433
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• 2010

Although tracking methods are efficient and popular for vessel segmentation, they require a seed to initiate an instance of tracking. In this paper, a new method to detect new seeds for tracking of arterial segments from CT angiography (CTA) and to construct a vascular structure is proposed. The proposed algorithm is based on shape analysis of connected components in a volume of interest around a vessel segment which was already extracted by tracking. The eigenvalues of the covariance matrix are used as the shape features for detection. The experimental results on actual clinical data showed that the results totally revealed the arterial tree not hindered by bone or veins. In visual comparison to a method which combines registration and subtraction of both pre-contrast and post-contrast CT volumes, the proposed method produced comparable results to the reference method and were confirmed of its feasibility for clinical use of reducing the cost and burden of patients.

• Journal of Ginseng Research
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• v.37 no.2
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• pp.254-260
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• 2013

Greenhouse and field experiments with American ginseng (Panax quinquefolius L.) stratified seed sown at depths of 10 to 100 mm were carried out to determine effects of seeding depth on seedling emergence, growth and development and to calculate optimum seeding depth. The time to 50% seedling emergence ($E_{50}$) in the field increased linearly from 17 d at 20 mm seeding depth to 42.5 d at 80 mm. Seedling emergence and root weight (economic yield) at the end of the first year each increased quadratically with the increase of seeding depth. Maximum emergence and root yields were produced at sowing depths of 26.9 and 30.6 mm respectively. In a greenhouse pot experiment, increasing seeding depth from 10 to 100 mm increased partitioning of dry matter to leaves from 23.6% to 26.1%, to stems from 6.9% to 14.2%, and decreased dry matter to roots from 69.5% to 59.7%. Optimum seeding depth was 31.1 mm for a corresponding maximum root weight of 119.9 mg. A predictor equation [X (seeding depth, mm)=Y (seed weight, mg)/9.1+20.96] for seeding depth for ginseng, based on data for ten vegetable crops, their seed weights and suggested seeding depths, predicted a seeding depth of 28.3 mm for ginseng similar to that reported above for most pot and field experiments.

• Journal of the Korean GEO-environmental Society
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• v.6 no.2
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• pp.5-14
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• 2005

In the present study, the liquefaction potential in the area of the Incheon international airport was assessed by applying the data of both standard penetration tests and laboratory tests to the modified Seed & Idriss method. The analysis was performed against the non-plastic silty soil layer and silty sand soil layer existing within the depth of 20m and under the ground water level, having the standard penetration value(N) of below 20. Also, each set of data was mapped using the GIS(Geographic Information System) and the safety factor against the liquefaction potential ($FS_{liquefaction}$) was obtained by overlapping those layers. Throughout the analysis, it was found that there exists a potential hazard zone for the liquefaction, showing partially that the safety factor against the liquefaction potential is 1.0 to 1.5 below the standard safety factor criterion. It is further thought to be necessary that the liquefaction potential for the corresponding hazard zone be additionally assessed in detail.

• Journal of Korean Society of Forest Science
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• v.107 no.3
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• pp.237-244
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• 2018

This study was carried out in order to secure basic data of seedling mass propagation technique of Rhododendron mucronulatum which is the native tree species of Korea by surveying the characteristics of its fruit and seed. The fruits were collected at Mt. Goryeo in Ganghwa-gun on different dates in 2013; August 26th, September 5th, September 12th, October 4th. The seed germination test was carried out at 5, 10, 15, 20, 25 and $30^{\circ}C$. Moisture content of the fruit was highest (54.5%) in the fruit collected on September 5th. Number of the seeds in a fruit was 91.3 to 116.3, regardless of the collection date. Seed length was highest ($1947.4{\mu}m$) in the seeds collected on October 4th and seed width was highest ($727.3{\mu}m$) in the seeds collected on September 12th. Germination rate of the seeds was highest at $25^{\circ}C$ regardless of the seed collection date, which showed the highest value(27.3%) in the seeds collected September 12th. Meanwhile, the seeds were not germinated not at all at 5, 10 and $30^{\circ}C$. $T_{50}$ and mean germination time of the seeds got shorter at the higher temperature. Germination uniformity got lower when the collection date got later. Germination speed of the seeds was fastest at $25^{\circ}C$. According to the results of this study, it seems that the appropriate time to collect fruit and seed is between September 12th and October 4th, and the appropriate temperature for the seed germination is $25^{\circ}C$.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.4
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• pp.463-469
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• 2010

Since MOLA(Mars Orbiter Laser Altimeter) data, which provides altimetry data for Mars, does not cover the whole Mars area, image matching with MOC imagery should be implemented for the generation of DEM. However, automatic image matching is difficult because of insufficient features and low contrast. In this paper, we present the area based semi-automatic image matching algorithm with MOC-NA(Mars Orbiter Camera ? Narrow Angle) imagery. To accomplish this, seed points describing conjugate points are manually added for the stereo imagery, and interesting points are automatically produced by using such seed points. Produced interesting points being used as initial conjugate points, area based image matching is implemented. For the points which fail to match, the locations of initial conjugate points are recalculated by using matched six points and image matching process is re-implemented. The quality assessment by reversing the role of target and search image shows 97.5 % of points were laid within one pixel absolute difference.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.431-435
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• 1987

Terminal bud removal has been thought as a useful practical management of soybean cultivation in Korea, and such cultivating method has been recommended till recent times. Many experiments about the effect of bud removal have been made, but it is not certain yet. This study was conducted to determine if source potential and seed yield would be affected by bud removal in 4 determinate soybean varieties. Terminal bud was removed by hand at the 5 trifoliolate stage of growth. Data were collected on leaf area and leaf dry matter weight from 58 to 101 DAP by nearly every 10 days and some agronomic characteristics and seed yield after maturity. Removing the apical bud did not increased leaf area and leaf dry matter. Number of modes and pods per a plant was increased by bud removal, but number of seeds per a pod was decreased, and seed yield was unchanged by such offsets. From the data, we concluded the source potential of soybean plants was not increased by terminal bud removal under the planting density, 22,000 pit/l0a, however it would be a useful way to reduce the lodging or over-growth.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.10
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• pp.843-852
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• 2016

The autorotative flight of maple seeds(Acer palmatum) is numerically simulated based on the 3D geometry and the motion parameters of real seeds. The nominal values of the motion parameters are 1.26 m/s for descent velocity, 133.6 rad/s (1,276 rpm) for spinning rate, $19.4^{\circ}$ for coning angle, and $-1.5^{\circ}$ for pitch angle. A compact leading-edge vortex (LEV) positioned at the inner span of the seed blade causes a large suction pressure on its leeward surface. The suction pressure peaks occur near the leading region of inner span sections. The flow pattern characterized by the prominent LEV and the values of aerodynamic force coefficients obtained in the present study are in good agreement with experimental data measured for a dynamically-scaled robot maple seeds. A spiraling vortex developed in the leeward region advances toward the seed tip and merges with the tip-passing flow, which is considered to be a mechanism of maintaining stable and attached LEV for the autorotating maple seeds.

• Nutritional Sciences
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• v.4 no.1
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• pp.13-19
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• 2001

This study was designed to investigate the effects of Coriandrum Sativum L. on lipid metabolism in rats with hypertriglyceridemia. Also, we compared the effects of the leaf, seed and root of Coriandrum Sativum L. on lipid metabolism in rats with hypertriglyceridemia. Hypertriglyceridemia was induced in 32 male Sprague-Dawley rats weighting 108$\pm$13 g through the feeding of high-fat diets containing 20% fat and 5% cellulose for ten days. The rats were divided into four groups as follows : control (C), leaf (L), seed (S) and root (R) groups. For the five-week experimental period, the C group was fed the above diet an the L, S and R groups were fed Coriandrum Sativum L. diets containing 5% dry leaf, seed and root of Coriandrum Sativum L., respectively. Intake of diet and weight gain were significantly lower in the C group than in the L, S and R groups. But there was no significant difference among the L, S and R groups. Because of weight differences among the groups, all obtained data was adjusted to weight. The level of plasma total cholesterol was not significantly different among the four groups. But after adjusting to weight differences, the level of plasma triglyceride was significantly higher in the C groups than in the L and S groups. These results suggest that dietary Coriandrum Sativum L. may increase appetite and have an inhibitory effect on the lipid metabolism of rats with hypertriglyceridemia. Also, those effects may be partly different (leaf, root and seed) from those of Coriandrum Sativum L.

• Animal cells and systems
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• v.13 no.2
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• pp.133-139
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• 2009

Green tea seed was extracted with absolute ethanol,and the green tea seed extract(GTSE)was subjected to assays for toxicity, antioxidant ability, angiogenesis inhibitory effects and cell adhesion, as well as western blotting, and an in vivo experiment against 4 high-ranking adult cancers in Korea. Our series of experimental data demonstrated that GTSE has an antioxidant ability superior to that of EGCG in the green tea leaf, and also exhibits a profound high tumor growth inhibitory activity on a variety of cancer cell lines, as well as nude mice infected with cancer cells. GTSE was identified as a natural anticancer compound showing excellent angiogenesis inhibition and cancer cell suppression abilities. Our preliminary observations also indicate that GTSE may be another potential source of natural dietary antioxidants and also may be applicable as a novel natural anticancer agent.