• 한국식품저장유통학회지
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• 제6권4호
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• pp.475-480
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• 1999

한국산 작두콩을 부위별(종자, 자엽, 껍질, 깍지, 잎, 줄기 및 뿌리)로 구분하여 화학성분을 분석한 결과는 다음과 같다. 작두콩의 부위별 일반성분 중 총당과 조단백질은 다른 부위에 비해 자엽에서 높게 나타났으며, 무기성분은 뿌리에서 칼륨, 잎에서는 칼슘의 함량이 높게 나타났으며, 유기산은 잎의 경우 oxalic acid가 2.556 mg%, 깍지는 citric acid가 573 mg%로 가장 높았다. 유리당은 껍질에서 sucrose가 3.80%, 줄기의 경우 fructose가 2.17%로 각각 높게 나타났다. 구성 아미노산은 자엽과 잎에서 glutamic acid가 각각 592 mg%와 429 mg%, 깍지에서 proline 497 mg%, 줄기에서 lysine 328 mg% 및 뿌리에서는 arginine이 714 mg%로 가장 높게 나타났다. 껍질과 깍지의 지방산 함량은 포화지방산인 경우 palmitic acid가 각각 32.75%와 21.93%이었으며, 불포화지방산의 경우는 linoleic acid가 각각 39.15%와 43.03%로 높게 나타났다.

• Molecules and Cells
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• 제42권9호
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• pp.646-660
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• 2019

Abscisic acid (ABA) is a phytohormone essential for seed development and seedling growth under unfavorable environmental conditions. The signaling pathway leading to ABA response has been established, but relatively little is known about the functional regulation of the constituent signaling components. Here, we present several lines of evidence that Arabidopsis Raf-like kinase Raf10 modulates the core ABA signaling downstream of signal perception step. In particular, Raf10 phosphorylates subclass III SnRK2s (SnRK2.2, SnRK2.3, and SnRK2.6), which are key positive regulators, and our study focused on SnRK2.3 indicates that Raf10 enhances its kinase activity and may facilitate its release from negative regulators. Raf10 also phosphorylates transcription factors (ABI5, ABF2, and ABI3) critical for ABA-regulted gene expression. Furthermore, Raf10 was found to be essential for the in vivo functions of SnRK2s and ABI5. Collectively, our data demonstrate that Raf10 is a novel regulatory component of core ABA signaling.

• 한국자원식물학회지
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• 제34권6호
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• pp.542-554
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• 2021

In this study, we cultivated 115 Soritae and 86 Yakkong black soybean landraces collected from ten different locations in Korea. Then, the variations of three yield components (one-hundred seeds weight (HSW), number of seeds per pod (SPP), and yield per plant (YPP)) and three anthocyanins (cyanidin-3-O-glucoside (C-3-O-G), delphinidin-3-O-glucoside (D-3-O-G) and petunidin-3-O-glucoside (Pt-3-O-G)) were investigated according to landrace type and collection area. Both yield components and anthocyanin contents significantly varied between the soybeans demonstrating genetic differences. Soritae landraces had the highest average HSW and TAC, whereas Yakkong landraces displayed the highest average SPP irrespective of collection area. Relatively, Yakkong landraces from Gyeongsangnam-do (1697.29 mg/100 g) and Soritae landraces from Gyeonggi-do (2340.94 mg/100 g) had the highest average TAC. Principal component analysis clearly separated Soritae and Yakkong landraces. Moreover, TAC and C-3-O-G showed positive and significant associations in both Soritae (r = 0.972) and Yakkong (r = 0.885) landraces, while yield components showed negative or weak correlations with each other. Overall, ten landraces were identified as important resources owing to their high yield (>150 g/plant) and high level of TAC (>2300 mg/100g). This study could lay foundations to molecular level investigations and reinforce the use of Yakkong and Soritae landraces during cultivar development.

• BMB Reports
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• 제42권9호
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• pp.541-551
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• 2009

Amyloidogenesis defines a condition in which a soluble and innocuous protein turns to insoluble protein aggregates known as amyloid fibrils. This protein suprastructure derived via chemically specific molecular self-assembly process has been commonly observed in various neurodegenerative disorders such as Alzheimer's, Parkinson's, and Prion diseases. Although the major culprit for the cellular degeneration in the diseases remains unsettled, amyloidogenesis is considered to be etiologically involved. Recent recognition of fibrillar polymorphism observed mostly from in vitro amyloidogeneses may indicate that multiple mechanisms for the amyloid fibril formation would be operated. Nucleation-dependent fibrillation is the prevalent model for assessing the self-assembly process. Following thermodynamically unfavorable seed formation, monomeric polypeptides bind to the seeds by exerting structural adjustments to the template, which leads to accelerated amyloid fibril formation. In this review, we propose another in vitro model of amyloidogenesis named double-concerted fibrillation. Here, two consecutive assembly processes of monomers and subsequent oligomeric species are responsible for the amyloid fibril formation of $\alpha$-synuclein, a pathological component of Parkinson's disease, following structural rearrangement within the oligomers which then act as a growing unit for the fibrillation.

• International Journal of CAD/CAM
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• 제5권1호
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• pp.19-27
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• 2005

As three-dimensional range scanners make large point clouds a more common initial representation of real world objects, a need arises for algorithms that can efficiently process point sets. In this paper, we present a method for extracting smooth surfaces from dense point clouds. Given an unorganized set of points in space as input, our algorithm first uses principal component analysis to estimate the surface variation at each point. After defining conditions for determining the geometric compatibility of a point and a surface, we examine the points in order of increasing surface variation to find points whose neighborhoods can be closely approximated by a single surface. These neighborhoods become seed regions for region growing. The region growing step clusters points that are geometrically compatible with the approximating surface and refines the surface as the region grows to obtain the best approximation of the largest number of points. When no more points can be added to a region, the algorithm stores the extracted surface. Our algorithm works quickly with little user interaction and requires a fraction of the memory needed for a standard mesh data structure. To demonstrate its usefulness, we show results on large point clouds acquired from real-world objects.

• 셀메드
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• 제10권2호
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• pp.11.1-11.14
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• 2020

Nigella sativa commonly known as Black seed, Black cumin or Kalonji (Family Ranunculaceae) is a widely used for its miraculous healing power. Use of N. sativa seeds and oil has splendid historical past in diverse traditional systems of medicine and food. In Tibb-e-Nabwi (Prophetic Medicine), it is considered as one of the greatest forms of healing medicine. Phytochemically; it contains fixed oil, protein, alkaloids saponin and essential oil. Therapeutic properties of this plant are due to the presence of thymoquinone which is one of major active component and has different beneficial properties. In Unani System of Medicine the diseases are treated with nontoxic herbal drugs. As per Unani classical literature N. sativa perform various pharmacological actions like carminative, anti-inflammatory, analgesic, diuretic, emmenagogue, galactagogue, expectorant etc. Ample of phytochemical, pharmacological and clinical researches has been executed on N. sativa., which may include antidiabetic, anticancer, immunomodulator, analgesic, antimicrobial, anti-inflammatory, bronchodilator, hepato-protective, renal protective, gastro-protective, antioxidant properties, etc. This review is an effort to summarize the literature on scientific researches of pharmacognostical characteristics, chemical composition and pharmacological activities of the kalonji seeds

• 한국작물학회지
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• 제48권
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• pp.41-48
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• 2003

The number of natural products obtained from plants has now reached over 100,000 and new chemical compounds are being discovered ever year. Medicinal and Aromatic plants and their extracts have been used for centuries to relieve pain, aid healing, kill bacteria and insects are important as the antifungal and anti-herbivore agents with further compounds being involved in the symbiotic associations. Although their functions in plants have not been fully established, it is Known that some substances have growth regulatory properties while others are involved in pollination and seed dispersal. The complex nature of these chemicals are usually produced in various types of secretory structures which is an important character of a plant family and also influenced and controlled by genetic and ecological factors. Detailed anatomical description of these structures ave relevant to the market value of the plants, the verification of authenticity of a given species and for the detection of substitution or adulteration. Volatile oils are used for their therapeutic action for flavoring of lemon, in perfumery of rose or as starting materials for the synthesis of other compounds of turpentine. For therapeutic purposes they are administered as inhalations of eucalyptus oil, peppermint oil, as gargles and mouthwashes of thymol and transdermally many essential oils including those of lavender, etc. With these current trend for using volatile components in essential oil will be increasing in the future in Korea and in the world as well.

• 한국식품과학회지
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• 제9권2호
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• pp.153-156
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• 1977

한국에서 재배된 해바라기(Helianthus annus)씨 단백질의 화학적 성질을 규명하고자 이의 염용해성 단백질을 추출한 다음 정제하여 주단백질을 분리하여 본 결과 1. 염용해성 단백질의 추출용매는 10% 염화나트륨을 함유한 0.02M sodium phosphate buffer가 가장 좋았다. 2. 해바라기씨 단백질의 필수아미노산 조성은 비교적 양질인 것으로 나타났다. 3. 염용해성단백질의 disc전기이동에서 7개의 band를 나타내었다. 4. 염용해성단백질의 주단백질은 Sephadex G-150과 A-25 column으로 정제하여 얻을 수 있었다. 4. 주단백질의 분자량은 약 86,000이였다.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.701-709
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• 2005

An efficient method of selecting an antagonistic strain for use as a biological control agent strain was developed. In this improved method, the surface tension reduction potential of an isolate was included in the 'decision factor,' in addition to two other factors; the growth rate and pathogen inhibition. By using a statistically designed method, an isolate from the soil was selected and identified as Bacillus sp. GB 16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth were observed when the Bacillus sp. GB 16 was used. The action of the surface tension reducing component was assumed to enhance the wetting, spreading, and residing of the antagonistic strain in the rhizosphere. This result showed that the improved selection method was quite effective in selecting the best antagonistic strain for the biological control of soilborne infectious plant pathogens.

• Journal of Applied Biological Chemistry
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• 제56권3호
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• pp.187-189
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• 2013

The antimicrobial activities of emodin and its derivatives (anthraquinone, alizarin, and alizarin-3-methyliminodiacetic acid) were evaluated using a paper disc diffusion method against foodborne bacteria (Bacillus cereus, Listeria monocytogenes, Staphylococcus intermedius, Salmonella typhimurium and Shigella sonnei). Emodin isolated from C. tora seeds has an antimicrobial activity against Bacillus cereus, followed by alizarin-3-methyliminodiacetic acid ($13.0{\pm}2.5mm$) and alizarin ($11.5{\pm}1.2mm$). Furthermore, emodin showed the antimicrobial activity against S. sonnei and S. typhimurium. In conclusion, C. tora seed and its active component derivatives are useful for the development of natural products on food supplemental agents and pharmaceuticals.