• 한국식품영양학회지
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• 제15권3호
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• pp.257-266
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• 2002

해조류 조직을 가수분해시켜 기호성 및 응용성이 높은 액상원료로 가공하는 기술 개발의 일환으로 우선 자연계에 존재하는 미생물로서 산, 논, 밭, 해변 및 어판장에서 약 200종류의 미생물군 시료를 수집하여 해조의 조직파괴가 인정되는 8군의 미생물 시료를 사용하여 돌가사리,톳 및 가시파래의 조직파괴와 가수분해 가능성에 대하여 검토하고,추출액 중의 전당과 환원당을 측정하여 추출율과 분해율을 비교하였다. 그 결과 돌가사리, 톳 및 가시파래의 전당 추출량과 추출율은 각각 담수에서 채취한 미생물군 시료(No.27번), 부식한 식물에서 채취한 미생물군 시료(No. 8번) 및 담수에서 채취한 미생물군 시료가 가장 높은 값을 나타내었고, 환원당의 추출량과 추출율에서는 각각 8번, 8번 및 27번 미생물군 시료가 가장 높은 값을 나타내었다. 분해율은 전당의 추출율도 상당히 높으면서 환원당의 추출이 가장 높았던 실험구들이 각각의 해조에서 가장 높은 분해율을 나타내는 결과를 얻었다. 따라서 이들 자연계에서 채취한 미생물군 시료중에 해조 조체를 파괴시키고, 또한 해조에 다량 함유되어 있는 당을 비롯한 유용성분의 추출을 용이하게 하며, 해조를 가수분해시키는 효소를 가진 미생물이 존재할 것으로 판단되었으며, 또한 이들 미생물군에서 단일 또는 복합 해조가수분해 미생물 및 효소의 생산이 가능할 것으로 기대되었다.

• 한국수산과학회지
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• 제41권1호
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• pp.68-72
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• 2008

The cellular viability of the human keratinocyte cell line HaCaT was compared after adding seaweed extracts to the culture medium. The viability was measured using a quick, quantitative, spectrophotometric crystal violet inclusion method. Of 36 common seaweed species tested, methanol extracts from Sargassum sagamianum and Gigartina tenella enhanced the viability of HaCaT cells by 1.6-fold, as compared to control cells, while methanol extracts from Dictyota dichotoma, Pachymeniopsis elliptica, and Enteromorpha linza decreased the viability to less than half that of controls.

• ALGAE
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• 제37권2호
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• pp.123-133
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• 2022

Genetic diversity and distribution patterns of marine macroalgae are increasingly being documented in Southeast Asia. These studies show that there can be significant levels of genetic diversity and isolation between populations on either side of the Thai-Malay Peninsula. Bostrychia tenellla is a common filamentous red seaweed in the region and the entity is represented by at least two cryptic species. Despite being highly diverse and widespread, genetic variation and population structure of this species complex remains understudied, especially around the Thai-Malay Peninsula. We analyzed genetic diversity and inferred the phylogeographic pattern of specimens identified as B. tenella using the plastid RuBisCo spacer from samples from the Andaman Sea and the Gulf of Thailand. Our genetic analysis confirmed the occurrence of the two cryptic B. tenella species (B and C) along both coasts. Cryptic species B was more common in the area and displayed higher genetic diversity than species C. Historical demographic analyses indicated a stable population for species B, but more recent population expansion for species C. Our analyses also revealed that both cryptic species from the Andaman Sea possessed higher genetic diversity than those of the Gulf of Thailand. We also detected moderate to high levels of gene flow and weak phylogeographic structure of cryptic species B between the two coasts. In contrast, phylogeographic analysis showed genetic differences between populations of both cryptic species within the Andaman Sea. Overall, these results suggest that cryptic B. tenella species around Thai-Malay Peninsula may have undergone different demography histories, and their patterns of genetic diversity and phylogeography were likely caused by geological history and regional sea surface current circulation in the area.

• 한국해양바이오학회지
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• 제4권1호
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• pp.31-34
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• 2010

The primary function of the skin is to protect the body from the unwanted environmental influences. The outermost layer of the skin is stratum corneum which consists of corneocytes surrounded by lipid regions. Ceramides covalently bound to keratinocytes are essential for the barrier function of the skin, which can be disturbed in the disease, like xerosis. Xerosis is an abnormal dryness of the skin which reduced the thickness of stratum corneum and ceramide content decreasing with age. In this study, 36 seaweed extracts have been tested for screening of xerosis inhibitory agent by in vitro HaCaT keratinocyte assay. Ishige sinicola and Helminthocladia australis induced the significant amount of ceramide-like substance I in HaCaT keratinocyte among the tested seaweed extracts. Sargassum fulvellum, Chondrus ecellatus and Gigartina tenella also induced the ceramide-like substance I whereas Helminthocladia australis and Pachymeniopsis elliptica induced the ceramide-like II from HaCaT keratinocyte.

• Ocean and Polar Research
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• 제26권1호
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• pp.59-64
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• 2004

As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for $ONOO^-$ and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at $5{\mu}g/ml$ of methanol extracts was observed in order of Ishige okamurae(95.3%), Sargassum hemiphyllum(90.2%), Symphyocladia latiuscula(89.6%), Porphyra suborbiculata(86.7%), and Gelidium amamsii(85.9%), Also, a significant scavenging effect against direct authentic peroxynitrite was revekaled for methanol extracts of Ishige okamurae(66.2%) and Sargassum hemiphyllum(55.2%) and the acetone/methylene chloride(1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride(1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.