• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.839-846
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• 1999

An extracellular chitinase-producing bacterial strain induced by colloidal chitin was isolated from sea sand and was identified to be a member of the genus Cytophaga. The chitinase was purified successively by 30-60% ammonium sulfate fractionation, and DEAE-Bio gel A column, Octyl-Sepharose CL-4B column, and DEAE-Bio gel A column chromatographies. The enzyme had a molecular mass of 59.75 kDa, and the amino terminal amino acid sequence was ATPNAPVISW MPTDXXLQNXS. The enzyme acted better on colloidal chitin as a substrate than on chitosan. For colloidal chitin and chitosan (Degree of Acetylation, 15-25%), $K_{cat}$ values were 0.60U/mg and 0.08U/mg, respectively. HPLC analysis of the enzymatic reaction products showed that the chitinase produced mostly N-acetyl-D-glucosarnine and di-N-acetylchitobiose. The optimum temperature and pH for the enzyme were $50^{\circ}C$ and 4.0, respectively. N-Bromosuccinimide and $Hg^{2+}$ inhibited the chitinase activity as much as 90%, and $Sb^{3+}$, diethylpyrocarbonate, and $Ag^{+}$ inhibited it by 50-70%.

• 한국해양공학회지
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• 제14권4호
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• pp.23-29
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• 2000

When the concrete structures were being made with sand containing chloride ion it was knows that corrosion rate of reinforced steel embedded in concrete with chloride ion was higher than that of concrete with on chloride ion. In this study, the operation of Friedel salts affecting the corrosion behavior of reinforced steel embedded in cement mortar was investigated with electrochemical view. Corrosion potential of reinforced steel embedded in cement mortar with sand containing chloride ion was shifted noble direction than that of cement mortar with no chloride ion after immersed 5 month in natural sea water and also corrosion current density decreased with shifting corrosion potential to noble direction. However Friedel salts appeared from surface to 2.5cm of inside direction of mortar specimen, which is located at 11.5$\circ$(2$\theta$) in XRD analysis and the amount of Ca(OH)2 by SEM photograph in cement mortar with chloride ion was larger than that of cement mortar with mo chloride ion. Eventually it is suggested that Friedel salts was resulted from chloride ion and it acted as the corrosion inhibitor.

• 미생물학회지
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• 제55권3호
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• pp.278-279
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• 2019

Actinobacteria 문 Beutenbergiaceae 과에 속하는 Miniimonas arenae KCTC $19750^T$는 해양모래에서 분리되었다. 본 연구에서는 KCTC $19750^T$의 비완전 유전체를 보고한다. 본 유전체는 3,402,690 bp의 크기와 73.6%의 평균 G + C 함량을 지니고 있으며, 2,947개의 단백질 코딩 유전자, 2개의 ribosomal RNA 및 44개의 transfer RNA로 구성되어 있다. 또한, 삼투압과 관련된 유전자를 포함하고 있다. 본 유전체 서열의 가용성은 Miniimonas 속의 유일한 구성원으로KCTC $19750^T$에 대한 더 많은 이해를 제공할 것이다.

• 한국해양학회지
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• 제29권3호
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• pp.217-227
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• 1994

한반도 서부대륙붕은 북동.남서방향의 불연속적인 사퇴가 잘 발달되어 있다. 사 퇴의 규모는 높이 3~21 m, 파장 3.1~6.8 km, 길이 9~64 km이며 경사도는 0.5이다. 이러한 사퇴들은 해수면상승에 따른 수심변화와 조류의 세기변화로 인하여 형성되고 변형 되어지는 것으로 해석된다. 사퇴의 특징은 다음과 같다. (1)사퇴의 발달이 조류 방향과 대체로 일치하는 북동-남서방향으로 발달하고 있다. (2) 사퇴의 내부는 3.5kHz 탄성파단면도와 주상퇴적물에 의해 두 개의 층(sequence)으로 구분된다. 상부층은 내 부 반사면이 없는 확산(prolonged type)된 음향상이 나타나며, 균질(massive)의 세립 사(Mx, 2-3$\psi$)로 구성되어 있다. 그 하부층은 수평내부반사(parallel internal reflector) 음향상이 나타나며, 엽리구조(kaminated structure)가 발달한 사질니 또는 니질사(Mz, 5-7$\psi$)로 구성되어 있다. 수평한 내부반사면은 사퇴의 양쪽 경사면에서 절 단(truncation)된 모습을 보이고 있다. (3) 비대칭형(asymmetrical type)의 모래파가 사퇴를 덮고 있다. 모래파의 급경사면이 사퇴의 서측은 동북동, 동측은 서남서방향을 향하고 있다. 반면, 사퇴의 정부는 대칭형(symmetrical type)의 모래파가 분포한다. 이러한 특징을 가진 사퇴의 기원은 하부퇴적층이 강한 조류에 의해 침식(scouring)되 어 등성이와 같은 지형을 형성하고, 침식되고 남은 조립질의 잔류퇴적물이 등성이의 상부를 피복하여 형성된 것으로 해석된다.

• 한국제4기학회지
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• 제17권1호
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• pp.27-37
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• 2003

한반도 동해 중부 해안에 위치한 강원도 고성군 문암리 신석기 유적지의 퇴적층의 퇴적환경은 퇴적물의 입도 특성과 광물 조성에 의하여 세 개의 퇴적층서 단위(Unit)로 구분되었다. 최하위 퇴적 층서 단위인 Uint 3은 하부의 사질층(S)과 상부의 니사질층(mS)으로 세분된다. 이 퇴적층은 해안 사구 모래가 퇴적된 후, 토양화 과정에 의해 소량의 니질(mud)을 함유하는 것으로 해석된다. Unit 3을 피복하는 Unit 2는 신석기 유물이 출토되며, 사층리 구조를 보이는 퇴적층으로 다시 하부의 니사질층(mS)과 상부의 사질층(S)으로 구분된다. 전체적으로 Unit 2 퇴적층은 해빈으로부터 바람에 의해 운반ㆍ퇴적된 전형적인 해안 사구(coastal dune) 퇴적층으로 해석된다. 최상위 퇴적층서 단위인 Unit 1은 역사질층(gS)으로 잔자갈이 함유되어 하부의 퇴적층보다 조립하며, 분급도 불량하다. 이 퇴적층은 해안사구 층으로 현재의 지표 유수와 식물의 영향을 받아 토양화 과정을 나타내는 것으로 해석된다. 이 문암리 유적지 사구층의 형성 연대는 기존의 해수면 변동 연구와 신석기 유물의 연대 분석 결과로 미루어 보아 현세 중기(middle Holocene, 약 7,800∼6,500 yr BP) 전후인 것으로 해석된다.

• Geomechanics and Engineering
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• 제22권2호
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• pp.173-185
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• 2020

Calcareous soil is originated from marine biogenic sediments and weathering of carbonate rocks. The formation history for calcareous sediment includes complex physical, biological and chemical processes. It is preferably selected as the major fill materials for hydraulic reclamation and artificial island construction. Calcareous sands possess inter pores and complex shape are liable to be damaged at normal working stress level due to its fragile nature. Thus, the engineering properties of calcareous soil are greatly affected by its high compressibility and crushability. A series of triaxial shear tests were performed on calcareous sands derived from South China Sea under different test conditions. The effects of confining pressure, particle size, grading, compactness, drainage condition, and water content on the total amount of particle breakage for calcareous soil were symmetrically investigated. The test results showed that the crushing extent of calcareous sand with full gradation was smaller than that a single particle group under the same test condition. Large grains are cushioned by surrounding small particles and such micro-structure reduces the probability of breakage for well-graded sands. The increasing tendency of particle crushing for calcareous sand with a rise in confining pressure and compactness is confirmed. It is also evident that a rise in water content enhances the amount of particle breakage for calcareous sand. However, varying tendency of particle breakage with grain size is still controversial and requires further examination.

• 수산해양기술연구
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• 제36권3호
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• pp.234-243
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• 2000

This paper is to study population ecological characteristics, including growth parameters, survival rate, instantaneous coefficients of natural and fishing mortalities, and age at first capture of the soft-shelled clam, Mya japonioa in the intertidal zone of South Sea in Korea. For describing growth of the clam a von Bertalanffy growth model was adopted, The von Bertalanffy growth curve had an additive error structure and the growth parameters estimated from a non-linear regression were SH/sub ∞/=79.83mm, K=0.26, and t/sub 0/= -0.01. Survival rate (S) of the soft-shelled clam was 0.26 (SD=0.02). The instantaneous coefficients of natural mortality (M) was estimated to be 0.78/year and fishing mortality (F) 0.57/year for the soft-shelled clam. The age at first capture (t/sub c/) was estimated as 2.69 year. The mean densities of the soft-shelled clam by bottom type were 3.40 inds./m²(SE=0.18) in the sand, 63.4 inds./m²(SE= 0.53) in the muddy sand, and 0 inds./m2 (SE=0) in the gravelly sand. The mean densities of the soft-shelled clam by 3 different areas were 4.88 inds./m²(SE=0.09), 2.61 inds./m²(SE=0.13), 7.20 inds./m²(SE=0.18), respectively and the biomass of the clam were estimated as 131mt, 121mt, 665mt, respectively. An yield-per-recruit analysis showed that the current yield-per-recruit of about 8.30g with F=0.57/year and the age at first capture (t/sub c/) 2.69 year, was lower than the maximum possible yield-per-recruit of 9.60g. Fixing to at the current level and increased fishing intensity (F) could produce an increase in the predicted yield-per-recruit from 8.30g to about 9.40. However, estimated yield-per-recruit increased to 1.30g by decreasing to from the current age (2.69 year) to age two with F fixed at the current level. Yield-per-recruit was estimated under harvest strategies based on F/sub max/ and F/sub 0.1/.

• 수산해양기술연구
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• 제48권3호
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• pp.208-216
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• 2012

The properties of sediment collected from seabed surface to 6cm depth on the four positions were analyzed to investigate turbulence of marine sediments by shrimp beam trawl. Types of sediments in the investigation area were (g)mS (slightly gravely muddy sand) and gmS (gravely muddy sand) showing high sand content, and (g)sM (slightly gravely sandy mud), gsM (gravely sandy mud) as well. It is estimated that position is more crucial factor than seasonal difference for the granularity variation of sediment in each investigation area. Finding the positional characteristics of sediment granularity was difficult before removing shells and organic matter. However, the average granularity is getting larger by going out from inland sea to open sea once those were removed. The granularity of marine sediment got narrow after processing in the fishing area for shrimp beam trawl but there was no big difference for granularity size before and after processing in the non-fishing area. This might be attributed to crushed shell particles going up and down again on the surface in the fishing area. To demonstrate the hypothesis mentioned above, the sediments driven by shrimp beam trawl need to be collected and analyzed.

• 한국수산과학회지
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• 제9권1호
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• pp.69-73
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• 1976

이질퇴적 백합 폐사에 미치는 영향을 구명하기 위하여 백합을 각 체장별로 구분한 후, 3종의 저질(사, 사이 및 이)과 5종의 구분으로 된 15개의 사육수조에 넣어 폐사율을 조사하였다. 1. 사이질처리군은 사질대조군과 마찬가지로 거의 폐사가 일어나지 않은 반면, 이질처리군은 폐사현상이 현저하였다. 2. 이질처리군의 경우, 폐사는 각장 2.5cm의 치패에서 가장 먼저 일어나고(34일), 폐사율도 가장 높은 (41일에 $85\%$) 반면, 성패에 이를수록 늦게 일어나고 폐사율도 낮았다. 3. 이질처리군의 폐사는 염분에 따라서도 차이가 있었다. 일반적으로 저염분에 이를수록 생존율은 높고, 고염분에 이를수록 낮았다. 4. 아가미 조직 산소소비율은 40일간의 이질처리군과 대조군간에 큰 차이가 없었다. 5. 논리적으로, 백합이 이질퇴적시에 받는 영향은 해수의 수온, 용존산소, 지반의 안정도, 해수중의 부이양 및 조개의 크기등에 따라서 차이가 있으나 상당한 시일이 경과하면 폐사현상이 초래되는 점은 분명하다.

• Weed & Turfgrass Science
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• 제7권1호
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• pp.54-61
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• 2018

Zoysiagrass (Zoysia spp.) exists spotly in Indonesia and it has potential to be used in parks, golf courses, and football fields. Many football fields and golf course fairways use sand as top soil over native soil. This study aims to analyze growth and quality of two native zoysiagrasses Zis and Zim. Zis is a native zoysiagrass collected in sea-side and Zim is a native zoysiagrass collected in mountain area. Both types of zoysiagrasses were planted at field with altitude of 300 m with various growing media mixes of sand and reservoir's sediment. Thickness of the growing medium was 10 cm over an alfisol clay soil. Experimental plots were constructed using factorial completely randomized design with two native zoysiagrasses and 5 types of growing media. Two ecotypes of native zoysiagrasses showed different in growth habits combined with mixtures of growth media. Zim showed higher growing speed including more vigor with uniformity and texture than Zis. There were not significanthly differences on leaf color and root length between two ecotypes of native zoysiagrasses collected in Indonesia.