• Title/Summary/Keyword: screening of fungi

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Recent trend of chemical studies of fungicide in China

  • Jiang, Mingliang
    • The Korean Journal of Pesticide Science
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    • v.6 no.1
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    • pp.1-8
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    • 2002
  • Recent progress of several kinds of compounds which have been synthesized or isolated from natural fungi for screening or conducting test as agricultural fungicides as well as some formulations for wood stain and decay control have been reviewed in this paper in China. Comments were pointed for Chinas further research and development of fungicides as well as pesticides.

Biotransformation of trans,trans-farnesol by Wood Rot Fungi (목재부후균에 의한 trans,trans-farnesol의 생물변환)

  • Kim, Young-Hun;Lee, Su-Yeon;Park, Mi-Jin;Choi, In-Gyu;Lee, Jae-Won
    • Korean Journal of Microbiology
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    • v.48 no.1
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    • pp.37-41
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    • 2012
  • In this study, we screened and evaluated possibility of wood rot fungi as biocatalyst for biotransformation of sesquiterpenes. Screening were performed to select the most promising microorganisms with ability to biotransformation the substrate trans,trans-fanesol. Trans,trans-farnesol which is synthesized precursor of sesquiterpenes was used for resistance test on 19 of wood rot fungi. From the 19 tested wood rot fungi, 5 were selected by resistance test on different concentration of trans,trans-fanesol. Biotransformation was performed with selected wood rot fungi on liquid culture. The metabolites detected by GC-MS analysis were nerolidol for Laetiporus sulphureus var. miniatus (jungh) Imaz and eicosane for Coriolus versicolor (L.Fr) Prlar and isoborneol for Fomitopsis pinicola and isocyclocitral for Lampteromyces japonicas. As the results, wood rot fungi could be potential biocatalyst for biotransformation of sesquiterpenes.

Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization

  • Furuta, Kazuyoshi;Nagashima, Saki;Inukai, Tsuyoshi;Masuta, Chikara
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.80-86
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    • 2017
  • One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge.

Screening of Botanicals Against Root Knot Disease Complex in Mulberry (Morus indica L.)

  • Naik V. Nishitha;Sharma D.D.;Govindaiah Govindaiah;Chowdary N.B.;Mala V.R.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.1
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    • pp.57-61
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    • 2007
  • For developing an integrated eco-friendly package against root knot disease complex of mulberry caused by the association of Meloidogyne incognita with Fusarium solani and F. oxysporum causing serious loss in terms of leaf yield and quality during cultivation, twenty botanical extracts at 5, 10 & 20% concentrations were screened under in vitro conditions. Among the extracts, Allium sativum followed by Lasownia inermis were found to be effective at 20% concentration against both the virulent fungi and nematode. Both the extracts reduced the mycelial growth of virulent fungi to an extent of 76-100%, inhibited the hatching of nematode eggs by 80-90% and 76-85% larval mortality over the control. The other extracts were found either moderately or poorly effective in reducing the growth of fungi, hatching of nematode eggs and enhancing the mortality of larvae. The two effective botanical extracts, which rated as strong inhibitors against both nematode and virulent fungi, can be utilized in developing an integrated ecofriendly technology for better management of root knot disease complex in mulberry.

An Antifungal Property of Burkholderia ambifaria Against Phytopathogenic Fungi

  • Lee Chul-Hoon;Kim Min-Woo;Kim Hye-Sook;Ahn Joong-Hoon;Yi Yong-Sub;Kang Kyung-Rae;Yoon Young-Dae;Choi Gyung-Ja;Cho Kwang-Yun;Lim Yoong-Ho
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.465-468
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    • 2006
  • Even though many pesticides are known for barley powdery mildew and wheat leaf rust, alternative controls are necessary, because of consumer rejection of chemical pesticides and the appearance of fungi resistant to fungicides. To discover biopesticides, many broths of microorganisms were screened. Of those, a culture broth of Burkholderia ambifaria showed an excellent antifungal activity against both Erysiphe graminis and Puccinia recondita, which cause barley powdery mildew and wheat leaf rust, respectively.

Fungicidal activities of leguminous seed extracts toward phytopathogenic fungi (콩과식물 종실 추출물의 살균활성)

  • Lee, Hoi-Seon;Kim, Byung-Sup;Kim, Heung-Tae;Cho, Kwang-Yun;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.2 no.3
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    • pp.21-27
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    • 1998
  • Methanol extracts from 25 leguminous seeds were tested for their fungicidal activities toward six phytopathogenic fungi, using whole plant test in a greenhouse. The efficacy varied with both the plant pathogen and legume species used. At 5 mg/pot, potent fungicidal activities were produced from extracts of Cassia obtusifolia, Glycine max var. solitae, G. max var. yagkong, G. max var. hooktae, Phaseolus multiflorus, P. radiatus var. aurea, and Vigna sinensis against Botrytis cinerea, Puccinia recondita, and Erysiphe graminis. These seed extracts were highly effective against three B. cinerea strains resistant to carbendazim, procymidone, and diethofencarb. All leguminous seed extracts revealed weak or no fungicidal effect against Rhizoctonia solani, Pyricularia grisea, and Phytophthora infestans. As a naturally occurring fungicide, leguminous seed-derived materials described could be useful as new fungicidal products against various plant diseases induced by phytopathogenic fungi.

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Studies on the Naringinase Produced by Aspergillus nidulans (Part I) Screening of Naringinase Produced by Fungi and Their Cultural Condition (Aspergillus nidulans가 생산하는 Naringinase에 관한 연구 (제1보) 생산균주의 선정 및 Naringinase의 생산조건)

  • 유주현;문순옥;변유량
    • Microbiology and Biotechnology Letters
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    • v.6 no.2
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    • pp.59-63
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    • 1978
  • The Screening of fungi producing naringinase was done. A strain of Aspergillus midulans showed the highest naringinase activity among 447 strains those were isolated from soil, spoiled citrus friuts and stock cultures. The cultural Conditions of Asp. nidulans for production of naringinase were studied. A strain of Asp. nidulans showed higher activity when it was cultivated at 30$^{\circ}C$ for 3 days on wheat bran media supplemented with 2.0% naringin, 0.2% (NH$_4$)$_2$ SO$_4$ and 0.2% CaCO$_3$.

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Studies on the Population of Toxigenic Fungi in Foodstuffs -IV. Screening test for strains that produce aflatoxin like substances- (각종(各種) 식품중(食品中)의 유독성(有毒性) 진균(眞菌)에 관(關)한 연구(硏究) -IV. Aflatoxin양물질(樣物質)의 분필균주(分泌菌株)의 검색(檢索)-)

  • Koh, Choon-Myung;Choi, Tae-Joo;Lew, Joon
    • The Korean Journal of Mycology
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    • v.1 no.1
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    • pp.17-21
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    • 1973
  • Fifty-eight strains of Aspergillus spp. isolated from various grains were examined for the screening of aflatoxins by the method of the Thin-Layer Chromatography (TLC), using the aflatoxin producing strain of Aspergillus flavus ATCC 15517 as a control. The results as follows: 1. Samples of Aspergillus spp. isolated from various grains were extracted with chloroform and chromatographied by the thin-layer chromatography method. 2. Three strains of Aspergillus spp. among the 58 strains can be found that the spots having the same Rf value as control with culture extract of Aspergillus flavus ATCC 15517. 3. The further prove studies of aflatoxins were proceed by the methods of in vivo and in vitro tests. And this methods considered to capable for the use of mass screening among the samples.

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