• Title/Summary/Keyword: science-related activity

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Antioxidant Enzyme Activity in Rat Liver and Kidney Related to Coix Intake

  • Kim, Kyeok;Lee, Mie-Soon
    • Preventive Nutrition and Food Science
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    • v.4 no.2
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    • pp.134-138
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    • 1999
  • The effects of dietary Coix(lacryma-jobi) water extract on the antioxidant enzyme activity in the liver and kidney of Sprague-Dawley rats were studied. Forty-five rats were fed for 3 weeks with either control diet or experimental diets that contain either Coix water extract or Coix water residue. Twenty percent of the carbohydrate was replaced with Coix water residue by dry weight in the water residue diet, while distilled water was replaced by Coix water extract to make a pellet-form diet in the Coix water extract diet. The levels of glutathione, glutathione-peroxidase, and glutathione-S-transferase activities in liver and kidney were measured . It has been found that glutathione, glutathione peroxidase, and glutathione-S-transferase enzyme activities from activities from liver and kidneyof the rats were enhanced in the group fed with Coix water extract.

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Factors Influencing Intention to Quit Smoking in Korean Adult Smokers: Using 2014 Korea National Health and Nutrition Examination Survey (우리나라 성인 흡연자의 금연의도와 관련 요인: 2014년 국민건강영양조사 자료를 이용하여)

  • Moon, Seongmi
    • Journal of Korean Biological Nursing Science
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    • v.19 no.3
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    • pp.178-190
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    • 2017
  • Purpose: The present study was designed to examine the impact of socioeconomic, health-related, and health behavioral factors on Korean people's intention to quit smoking. Methods: The subjects were 851 men and 145 women, aged 19 or more who had participated in the 2014 Korea National Health and Nutrition Examination Survey. The intention to quit smoking was categorized as "within 6 months," "after 6 months," or "no intention". The associations of the intention to quit smoking with sociodemographic, health related, and health behavioral factors were examined in a logistic regression. Results: In male smokers, the number of diseases, perceived somatotype, vigorous intensity leisure activity, aerobic physical activity, and smoking intensity had a significant influence on the intention to quit smoking. In female somkers, the economic status, number of diseases, walking activity, and moderate intensity leisure activity were significantly influential on the intention to quit smoking. Conclusion: To increase male smokers' intention to quit smoking, it should be emphasized that smoking is harmful to. Moreover, special attention should be paid to low-educated female smokers to increase their intention to quit smoking.

EVIDENCE FOR THE SOLAR CYCLE IN THE SUNSPOT AND AURORA RECORDS OF GORYER DYNASTY (고려시대의 흑점과 오로라 기록에 보이는 태양활동주기)

  • YANG HONG JIN;PARK CHANGBOM;PARK MYUNG GOO
    • Publications of The Korean Astronomical Society
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    • v.13 no.1 s.14
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    • pp.181-208
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    • 1998
  • We have analyzed the sunspot and aurora data recorded in Go-Ryer-Sa. We have collected 35 records of sunspot observations for 46 days, and 232 records of auroral observations. To objectively estimate the periods of the solar activity appearing in these records a method of calculating the one-dimensional power spectrum from inhomogeneous data is developed, and applied to the sunspot and auroral data. We have found statistically significant 10.5 and 10 year periodicities in the distributions of sunspot and aurora records, respectively. These periods are consistent with the well-known solar activity cycle. There are indications of the long-term variations, but the period is not certain. We have also calculated the cross-correlations between the sunspot and auroral data. In particular, we have divided the aurora data into several subgroups to study their nature. We conclude that the historical records of strong auroral activity correspond to non-recurrent magnetic storms related to the sunspots. On the other hand, the records of weak auroral activity are thought to be related with the recurrent magnetic storms which occur frequently due to the coronal hole near the sunspot minimum.

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Relationship Between Relative Water Content and Ascorbate Redox Enzymes Activity in Lettuce Leaves Subjected to Soil Water Stress (토양 수분 Stress에 따른 상추의 엽중 상대수분 함량과 아스코브산 관련 효소 활성도)

  • Kang, Sang-Jae;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.32-39
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    • 2013
  • The relationship between relative water contents of lettuce leaves and biochemical activities in lettuce was examined in this study to explore an adaptation response of lettuce to water stress from soils. Soil water contents and relative water contents of leaves were positively related to show $R^2$=0.8728. Hydrogen peroxide contents of leaves rapidly increased with reduction of soil water content, whereas soluble protein contents and dry matters rapidly decreased. And chlorophyll a and b contents of leaves decreased with increase in carotenoid content. Furthermore, the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) increased dramatically, and mRNA transcript levels of APX, MDHAR and DHAR also increased. Relationship of relative water content of lettuce leaves to hydrogen peroxide, to ascorbate peroxidase activity, to dehydroascorbate reductase activity, and to monodehydroascorbate reductase activity was shown to be positively correlated. It is highly plausible from this study that these enzyme activities could be developed as an indicator of water states in soils.

Factors of Students' Career Choice Related to Science (초.중.고 학생들의 과학 관련 진로 선택 요인)

  • Yoon, Jin
    • Journal of The Korean Association For Science Education
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    • v.22 no.4
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    • pp.906-921
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    • 2002
  • The purpose of this study was to survey the students' career choice related to science. Important factors of career choice were identified through factor analysis. 'Perception of career related to science', 'preference for science learning' and 'participation in science-related activity' were three main factors of science-related career choice. Students' responses to the three main factors were compared according to their career choice, grade and gender using ANOVA. Regression analysis was adopted to find out the relative importance among the three main factors. The subjects were 947 grade 6, 9 and 11 students in Seoul. Numbers of boys and girls in each grade was almost same. The questionnaire was developed to know the factors of students' science-related career choice after preliminary research and literature survey. The ratio of science-related career choice was not high (26%). Students' responses to and the relative importance of the three main factors differed with the grade and gender. From the results, making students have preference for science and giving them more opportunities of science-related activity is more important than making them have positive perception of science-related career. It is required to make a material for science career education considering the differences of age and gender using this study results.

Antioxidant and Whitening Effects of Agrimonia pilosa Ledeb Water Extract (짚신나물 물 추출물의 항산화 활성 및 미백효과에 관한 연구)

  • Kim, Tae-Hyuk;Kim, Jeong-Mi;Baek, Jong-Mi;Kim, Tae-Woo;Kim, Dae-Jung;Park, Jeong-Hae;Choe, Myeon
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.3
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    • pp.177-184
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    • 2011
  • This study was performed to assess the antioxidant activities and whitening effects of Agrimonia pilosa Ledeb on melanin synthesis. The whitening effects of Agrimonia pilosa Ledeb water extracts were examined by in vitro mushroom tyrosinase assay and B16BL6 melanoma cells. We assessed inhibitory effect of Agrimonia pilosa Ledeb water extract on expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2) in B16BL6 cells. Inhibitory effect of Agrimonia pilosa Ledeb onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activitie. Our results indicated that Agrimonia pilosa Ledeb water extract effectively inhibited free radical generation. In DPPH and hydroxy radical scavenging activity, Agrimonia pilosa Ledeb water extract had a potent anti-oxidant activity in a dose-dependent manner. They significantly inhibited tyrosinase activity in vitro and in B16BL6 melanoma cells. Also, Agrimonia pilosa Ledeb suppressed the expression of tyrosinase in B16BL6 melanoma cells. These results show that Agrimonia pilosa Ledeb inhibited melanin production on the melanogenesis. The underlying mechanism of Agrimonia pilosa Ledeb on whitening activity may be due to the inhibition of tyrosinase activity. We suggest that Agrimonia pilosa Ledeb may be useful as new natural active ingredients for antioxidant and whitening cosmetics.

Inhibitory Effect of Polyporus umbellatus Extract on Melanogenesis (저령 추출물의 멜라닌 생성억제 작용)

  • Kang, Lea Minju;Park, Seol-a;Mun, Yeun-Ja;Woo, Won-Hong
    • Korean Journal of Acupuncture
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    • v.37 no.1
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    • pp.24-30
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    • 2020
  • Objectives : The purpose of this study was to investigate melanogenesis inhibition of ethanol extract of Polyporus (EP) by using B16F10 melanoma cells. Methods : We measured antioxidant effect of EP by using 1,1-Diphenyl-1-picrylhydrazyl (DPPH) assay and we confirmed melanin contents and tyrosinase activity of EP in cells. Additionally, the expression of tyrosinase-related protein-1 (TRP-1) and TRP-2 was observed by Western blot. Results : EP showed significantly high radical scavenging activity and inhibition of melanogenesis in dose-dependent manner by decreasing cellular tyrosinase activity and melanin content with or without α-melanin stimulating hormone. TRP-1 and TRP-2 expressions were also suppressed by EP in B16F10 cells. Conclusions : These results suggest that EP inhibits the melanogenesis and it could be a new organic ingredient for hyper-pigmentation.

Phosphoryl Transferring Activity was Revealed from $F_1-ATPase$ of Escherichia coli by $^{31}P$ NMR Investigation

  • Sohn, Joon-Hyung;NamKung, Jun;Yoon, Joon-Ho;Woo, Mi-Kyoung;Yeh, Byung-Il;Choi, Jong-Whan;Kim, Hyun-Won
    • Biomedical Science Letters
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    • v.13 no.3
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    • pp.169-173
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    • 2007
  • [ $^{31}PNMR$ ] spectroscopy revealed the adenylate kinase-like activity and the phosphotransferase activity from $F_1-ATPase$ of Escherichia coli. Incubation of $F_1-ATPase$ with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting the generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1-ATPase$ with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase activity of $F_1-ATPase$. Both adenylate kinase-like activity and the phosphotransferase activity has not been reported from $F_1-ATPase$ from Escherichia coli. $^{31}P$ NMR proved that it could be a valuable tool for the investigation of phosphorous related enzyme.

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Study on the Anti-oxidant and Cosmeceutical Activity of Taraxacum platycarpum (포공영(浦公英)의 항산화 및 화장품약리학적 활성 연구)

  • Cheon, Soon-Ju;Joe, Woo-A;Kim, Young-Hun;Jang, Min-Jung;Sung, Ji-Yeun;Kang, Bo-Yeon;Choi, Eun-Young;Son, Jun-Ho;Back, Wook-Jin;Lee, Chang-Eon;An, Bong-Jeun;Lee, Jin-Tae
    • The Korea Journal of Herbology
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    • v.21 no.4
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    • pp.109-113
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    • 2006
  • Objectives : In this study, anti-oxidant activity of Taraxacum platycarpum is confirmed to investigate cosmeceutical activity for utilization as a cosmetic ingredients. Methods : In the anti-oxidant and whitening effect experiment, the electron donating ability of Taraxacum platycarpum extracts, their SOD-like activity, and the inhibitory activity of xanthine oxidase, tyrosinase were examined. Results : Radical-scavenging activity of water and ethanol extract was examined using ${\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl$ (DPPH). Water and ethanol extract from Taraxacum platycarpum showed 62.9% and 54.7% at 500 ppm concentration in DPPH radical inhibition, respectively. In the test of superoxide dismutase (SOD)-like activity, water extract showed 63.4% at 1,000 ppm concentration, while ethanol extract showed 49% at 1,000 ppm concentration. In the test of xanthine oxidase inhibition, ethanol extract showed 66.8% at 1,000 ppm concentration. Tyrosinase inhibition effect related to whitening effect showed 36.3% and 54.2% in ethanol extract and water extract at 1,000 ppm concentration, respectively. Conclusion : According to these results, it is possible that the extract of Taraxacum platycarpum can be used as a new natural material of cosmetic industry.

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Comparison of Superoxide Dismutase and Peroxidase Activities in Rice Varieties

  • Chung, Ill-Min;Kim, Kwang-Ho;Ahn, Joung-Kuk;Lee, Jin-Ohk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.277-281
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    • 2000
  • Fifty-four Korean native and 28 foreign varieties harvested in 1998 and 1999 were examined for superoxide dismutase (SOD) and peroxidase (POD) activities. The SOD and POD activities of leaves extracts in Korean native and foreign rice varieties showed variation at the heading stage. The activities of SOD and POD changed with growth stage. In comparison of storage duration, the SOD and POD activities of the extract from three months stored seeds in Korean native (CV=53.3%) and foreign rice (CV=57.9%) varieties were higher than that of stored rices for a year in seed extracts although the activities among varieties did not show significant variation. Also, the averaged activity of foreign rice varieties (SOD=12.9%) was relatively higher than that of korean native rice varieties (SOD=10.7%). The test of activity at the enzymatic level related to antioxidative activity suggests that the rice varieties with higher antioxidative potentials can be developed and also may provide information with rice breeder to breed rice variety with a high antioxidative activity.

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