• Title/Summary/Keyword: science aspiration

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Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos II. Nuclear transfer using donor embryos originated from ovum pick-up(OPU) and activated recipient cytoplasts (핵이식 기법을 이용한 한우 쌍태생산에 관한 연구 II. Ovum pick-uo(OPU) 유래 공여핵 및 활성화 유도 수핵난자의 핵이식)

  • Hwang, Woo-suk;Shin, Tae-young;Roh, Sang-ho;Park, Jong-im;Lee, Byeong-chun
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.652-658
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    • 1998
  • The efficiency of nuclear transfer using donor embryos originated from ovum pickup(OPU) and activated recipient cytoplasts were examined for induction of twinning in Korean native cattle(KNC). After aspiration of follicle by OPU, regardless of the vacuum applied, we obtained same result in proportions of recovered cumulus-oocyte complex (COCs) with compact cumulus. Under electric stimulation(1.0kV/cm DC for $40{\mu}s$), most of activated oocytes proceed to anaphase II/telophase II within 3h(84.7%). In the treatment of oocyte activation, the preactivation which was performed before fusion had significant effect on the developmental rates to morula/blastocyst stage(9.4 vs 4.0%). In embryo transfer of nuclear transferred embryos, we obtained 2 twins from KNC recipients and 1 twin from a Holstein recipient. Our results showed that it is possible to obtain twins using nuclear transfer technique in KNC.

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Alimentary Lymphoma in a ferret (페렛에 발생한 소화기 림프종 1례)

  • Seo, Kyoung-won;Lee, Jong-bok;Jee, Hyang;Lee, So-ra;Kwon, Tae-uk;Kim, Dae-yong;Youn, Hwa-young;Lee, Chang-woo
    • Korean Journal of Veterinary Research
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    • v.47 no.4
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    • pp.479-482
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    • 2007
  • A 2-year-old spayed female ferret with the clinical signs of diarrhea and anorexia for about 8 days was presented to Hansung animal hospital. The diarrhea was black and paste form. Three palpable abdominal masses were detected in physical examination. By cytologic examination using fine need aspiration, the patient was tentatively diagnosed as lymphoma. Chemotherapy was started with prednisone, vincristine and cyclophosphamide. However, the client requested stopping the therapy at day 18 and the ferret was euthanized. Autopsy was performed and the masses were confirmed as lymphoma by histopathology. Microscopically, the mass consisted of unencapsulated and infiltrative neoplasm consisted of closely packed monomorphic round cells and also ininvasive lesions observed in duodenum. On the higher magnification, pleomorphic neoplastic cells with distinct cell border and abundant eosinophilic cytoplasm were observed. Finally, the ferret diagnosed as alimentary lymphoma.

HUGE PLEOMORPHIC ADENOMA OF THE PAROTID GLAND: REPORT OF A CASE (이하선에 발생한 거대 다형성 선종)

  • Ryu, Sun-Youl;Ryu, Seung-Hee;Kim, Tae-Hee
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.3
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    • pp.247-253
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    • 2006
  • Pleomorphic adenoma of the parotid gland typically presents as painless, mobile mass of long duration, and originate in the superficial lobe but, more rarely these tumors may involve the deep lobe of the parotid gland. The average size of a parotid neoplasm is 2 to 4 cm. The effective treatment of parotid pleomorphic adenoma is surgical excision. The simple excision or enucleation resulted in recurrence rate of 45% in benign tumor. Therefore, the superficial parotidectomy with identification and preservation of the facial nerve is now the most widely accepted surgery. We report a case of the huge pleomorphic adenoma of the left parotid gland in a 67-year-old man who complained the large mass, measured about $10\times7\times5$cm-sized, in front of the left ear and on the mandibular ascending ramus. The diagnosis was confirmed by the clinical examination, computed tomographic scan, fine needle aspiration, and incisional biopsy. Superficial parotidectomy including the tumor and preservation of the facial nerve using the modified Blair approach was performed. And satisfactory results have been obtained cosmetically and functionally.

A Design of KP AGENT for Intelligent Information Retrieval (지능형 정보검색을 위한 KP AGENT의 설계)

  • 박경우;배상현
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.4 no.2
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    • pp.443-451
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    • 2000
  • Until now, there have been various kinds of science information databsae which databased the science technology information, but they do not satisfy the aspiration of the users. Therefore, in the position of the users, it suggests the technology information space as a now paradigm, which supplement the function of science information DB. ICPIS which inputs described papers with keywords, offers the itemized summary of these contents, the visual indication and comparison of similar thesis, and it also supplises the abundant summary information, survey information, more than ten volumes of info communication thesis with starting the casual relation extraction for the users, playing a significant role in ICPIS is called KP, and it is package of domain knowledge that unifies the extraction and structure narration of the technology information. ICPIS extracts the technology information among the thesis that are deserved by the natural language treatment in the itemized KP keywords described, and form the prescribed summary structure in KP.

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Application of Immunophenotyping and Heteroduplex Polymerase Chain Reaction (hPARR) for Diagnosis of Canine Lymphomas

  • Sirivisoot, Sirintra;Techangamsuwan, Somporn;Tangkawattana, Sirikachorn;Rungsipipat, Anudep
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.6
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    • pp.2909-2916
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    • 2016
  • Background: Canine malignant lymphoma is classified into B- or T-cell origin, as in the human case. Due to differences in prognosis, a suitable method needs to be developed for lineage identification. Aims: To determine the accuracy of immunophenotypic and molecular information between three methods: immunocytochemistry (ICC), immunohistochemistry (IHC) and heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) in spontaneous canine lymphomas. Materials and Methods: Peripheral blood, fine needle aspiration and tissue biopsies from enlarged peripheral lymph nodes prior to treatment of 28 multicentric lymphoma patients were collected. Cytopathology and histopathology were examined and classified using the updated Kiel and WHO classifications, respectively. Anti-Pax5 and anti-CD3 antibodies as B- and T-cell markers were applied for immunophenotyping by ICC and IHC. Neoplastic lymphocytes from lymph node and white blood cell pellets from peripheral blood were evaluated by hPARR. Results: In this study, low grade B-cell lymphoma accounted for 25% (7/28), high grade B-cell lymphoma for 64.3% (18/28) and high grade T-cell lymphoma for 10.7% (3/28). According to the WHO classification, 50% of all cases were classified as diffuse large B-cell lymphoma. In addition, ICC showed concordant results with IHC; all B-cell lymphomas showed Pax5+/CD3, and all T-cell lymphomas exhibited Pax5-/CD3+. In contrast to hPARR, 12 B-cell lymphomas featured the IgH gene; seven presented the $TCR{\gamma}$ gene; five cases showed both IgH and $TCR{\gamma}$ genes, and one case were indeterminate. Three T-cell lymphomas showed the $TCR{\gamma}$ gene. The percentage agreement between hPARR and ICC/IHC was 60%. Conclusions: Immunophenotyping should not rely on a single method. ICC or IHC with hPARR should be used concurrently for immunophenotypic diagnosis in canine lymphomas.

Vascular ring anomaly with a right patent ductus arteriosus and a left aortic arch in a juvenile cat

  • Aki Takeuchi ;Kazumi Shimada ;Lina Hamabe ;Tomohiko Yoshida;Yusuke Ozai ;Miki Hirose ;Aimi Yokoi ;Momoko Watanabe ;Ikki Mitsui ;Ryou Tanaka
    • Journal of Veterinary Science
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    • v.24 no.4
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    • pp.50.1-50.6
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    • 2023
  • This paper reports the clinical findings and surgical treatment of feline right patent ductus arteriosus (RPDA) with a left aortic arch. A two-month-old female Maine Coon was referred for an investigation of regurgitation after weaning. RPDA with a left aortic arch was diagnosed based on the echocardiographic and computed tomography (CT) findings. A right-fourth intercostal thoracotomy was found to be an appropriate approach to the duct. Preoperative diagnosis is crucial and diagnostic imaging, including radiography, echocardiography, and cardiac CT examination, is essential for determining if the aortic arch is right or left.

Imaging of Acute Pulmonary and Airway Diseases in Children (소아의 급성 폐 및 기도질환의 영상)

  • Mi-Jung Lee;Hyun Joo Shin;Haesung Yoon
    • Journal of the Korean Society of Radiology
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    • v.81 no.4
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    • pp.756-769
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    • 2020
  • Among the various emergency diseases in children, acute pulmonary and airway disease are common clinical conditions encountered by radiologists, and the first imaging modality is chest radiography. Therefore, it is important to be familiar with these diseases and their imaging findings. In this article, we review pneumonia and mimickers of acute pulmonary disease. For acute airway disease, we reviewed croup, acute epiglottitis, tracheomalacia, asthma, postinfectious bronchiolitis obliterans, and foreign body aspiration. We hope this review of special diseases can help the diagnosis and treatment in children.

Relationship between Science Academic Passion, Positive Experience about Science and Scientific Creativity in Elementary Science-Gifted Students (초등 과학영재 학생의 과학 학업 열정 및 과학 긍정 경험과 과학적 창의성의 관계)

  • Kang, Hunsik
    • Journal of Korean Elementary Science Education
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    • v.42 no.3
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    • pp.455-466
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    • 2023
  • This study explored the relationship between science academic passion, positive experience about science and scientific creativity in elementary science-gifted students. To do this, 108 science-gifted students from grades 3 to 6 were selected. After conducting the tests on their science academic passion, positive experience about science and scientific creativity, descriptive statistics, correlation analysis, and multiple regression analysis were conducted. The results revealed that the students exhibited relatively high levels of science academic passion and positive experience about science, but their scientific creativity was not relatively high. While there was no statistically significant correlation between the overall science academic passion and scientific creativity, there was a significant negative correlation with scientific creativity in the aspect of 'obsessive passion' of the five subcategories ('importance', 'like', 'time/energy investment', 'harmonious passion', and 'obsessive passion'). Furthermore, the five subcategories, particularly 'like', 'harmonious passion', and 'obsessive passion' were statistically significant predictors of scientific creativity. However, the five subcategories of positive experience about science ('science academic emotion', 'science-related self-concept', 'science learning motivation', 'science-related career aspiration', and 'science-related attitude') did not exhibit statistically significant correlations with scientific creativity and did not had a significant influence on it. Additionally, neither the overall science academic passion nor the overall positive experience about science had a statistically significant effect on scientific creativity. Educational implications of these results were discussed.

A Study on the Incidence of Thyroid Cancer in Gender Ultrasound (초음파검사에서 성별에 따른 갑상샘 암의 빈도에 관한 연구)

  • Kwak, Eun-Suk;Lim, Cheong-Hwan;Yang, Oh-Nam
    • Journal of radiological science and technology
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    • v.38 no.2
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    • pp.145-153
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    • 2015
  • Thyroid nodules are an endocrine disease often found in clinical practice, and patients with thyroid nodules found by chance have rapidly increased alongside development of thyroid ultrasound techniques for health examination purposes. This study analyzes the subjects' general characteristics, thyroid ultrasounds, and fine needle aspiration cytology in order to find out the relationship between male and female thyroid nodules and thyroid cancer frequency. An ultrasound examination of the thyroid was performed for 32,973 individuals who visited the K Hospital of Health Examination. Subjects have no history of thyroid disease and are 20 years old or over. Data of general characteristics, diabetes) was collected by a written survey completed by the subject, and the ultrasound of the thyroid(thyroid nodules existence, size, number) and FNAC was used to find out the malignancy rate. Frequency of patients with thyroid nodule was 4,611(26.1%) in men and 5,341(34.9%) in women between 32,973 individuals. Women's prevalence rate is significantly higher than men, and the prevalence rate significantly increased with age in men and women(p < 0.05). The prevalence of multiple nodules was significantly higher in women(43.5%) than in men(35.6%), and significantly increased with age in men and women(p < 0.05). The fine needle aspiration cytology was performed in 692(men 342, women 350) subjects who showed signs of malignancy through ultrasound. Prevalence of malignancy of the nodules was higher in men(33.3%) than in women(29.4%) although it is not statistically significant. It is known that thyroid nodule prevalence in women is much higher than in men. But this study shows the men's prevalence rate was not too low compared with women, and the men showed a rather higher malignancy rate in nodules than women. It is considered that the role of thyroid ultrasound is both important in men and women.

Girls Left Behind in Science Gifted Education?: Gender Differences in Science Affective Domains among Top 10% High Achievers in PISA 2006 (최우수 여고생은 과학영재교육의 소외 집단인가? : PISA 문항의 과학성취도 상위 10% 고등학생의 과학 정의적 영역의 성차 분석)

  • Seo, Hae-Ae
    • Journal of Gifted/Talented Education
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    • v.21 no.1
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    • pp.123-139
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    • 2011
  • The research paid attention to the fact that top 10% high achievers of girls in high schools are not equally provided with opportunities for science gifted education in Korea. For this reason, the research examined gender differences in science achievement and science affective domain through employing PISA 2006 science achievement test and survey of science affective domains. The research subjects of 132 students as top 10% high achievers extracted from those 1,409 students who were nationwidely sampled and responded to the science achievement test and science affective domain survey in July 2008. The responses by 132 students of top 10% high achievers were analyzed. The findings revealed that there is no significant difference of science achievement and most items of science affective domain survey between girls and boys of top 10% population in high schools. Further, it was indicated that top 10% high achieving girls have interest, self-concept, self-efficacy, future job aspiration in science as high as boys. In conclusions, further attentions to provide more opportunities of science gifted education for girls are called for.