• Title/Summary/Keyword: scanning microscopy

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Microscopic Feature, Protein Marker Expression, and Osteoinductivity of Human Demineralized Dentin Matrix

  • Park, Sung-Min;Hwang, Jung-Kook;Kim, Young-Kyun;Um, In-Woong;Lee, Geun-Ho;Kim, Kyung-Wook
    • Journal of Korean Dental Science
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    • v.5 no.2
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    • pp.77-87
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    • 2012
  • Purpose: This study examined the scanning electron microscopic feature, protein marker expression and osteoinductive activity of demineralized dentin matrix (DDM) from human for nude mice. Materials and Methods: Twenty healthy nude mice, weighing about 20 g were used for study. DDM from Human was prepared and implanted into the dorsal portion of nude mouse. Before implantation, DDM was examined by scanning electron microscopy (SEM). Nude mice were sacrificed at 2 weeks, 4 weeks and 8 weeks after DDM grafting and evaluated histologically by H-E, MT staining. And also immunohistochemistry analysis (ostecalcin, osteopontin) was performed. Result: Dentinal tubules and collagen fibers were observed by SEM of dentin surface of DDM. The DDM induced bone and cartilage independently in soft tissues. And, the histological findings showed bone forming cells like osteoblasts, fibroblasts at 2, 4 and 8 weeks. On immunohistochemistry analysis, osteocalcin and osteopontin positive bone forming cells were observed. Conclusion: This results showed that the DDM from human has osteoinductive ability and is a good alternative to autogenous bone graft materials.

Current Trend of Ultrahigh Vacuum Low Temperature Scanning Tunneling Microscopy (초고진공 저온 주사터널 현미경 장치의 최신 경향)

  • Ham, Ungdon;Yeom, Han Woong
    • Vacuum Magazine
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    • v.3 no.4
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    • pp.14-18
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    • 2016
  • In this article, we will summarize recent advances in ultrahigh vacuum (UHV) low-temperature scanning tunneling microscopy (STM) during the last decade. Leading STM groups have finished or are constructing UHV milli-Kelvin high magnetic field STM capable of a few tens of milli-Kelvin and ~ 10 tesla. Applications with UHV sub-Kelvin high magnetic STM have been increased since mid-2000's. Active research using UHV low temperature tuning fork atomic force microscopes and UHV photon low-temperature scanning tunneling microscopes will be introduced. Considering these advances of UHV low-temperature STM we will discuss next trend in STM in the near future.

Current-Voltage Properties measuring of Dipyridinium Molecule Using Scanning Tunneling Microscopy (STM에 의한 Dipyridinium 분자의 전압-전류 특성 측정)

  • Lee, Nam-Suk;Shin, Hoon-Kyu;Chang, Jeong-Soo;Kwon, Young-Soo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.11a
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    • pp.485-488
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    • 2004
  • 본 연구에서는 dipyridinium dithioacetate 분자를 Au(111) 표면에 자기조립하여 STM 탐침-유기 단분자막-Au(111)기판의 수직구조로 STM 측정시스템을 이용하여, 전기적 특성을 관찰하였다. 먼저 Au(111)기판을 Piranha용액$(H_2SO_4:H_2O_2=3:1)$으로 Au 표면을 전처리 하였다. 전처리한 Au(111) 기판을 dipyridinium dithioacetate 1mol/ml 농도로 자기조립 하였으며, 자기조립막의 표면 구조를 STM으로 관찰하였다. dipyridinium dithioacetate의 전기적 특성은 STM 탐침-유기단분자막-Au(111) 기판의 수직구조로 STS를 이용하여 조사하였다. 전압과 전류 측정에서 전압이 증가함에 따라 전류가 감소하는 부성 미분저항(NDR)의 특성이 관찰 되었다. NDR 수치가 $-545\;[m\Omega/cm^2]$였고, PVCR은 1.64:1 이었다.

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MORPHOLOGICAL STUDY BY SCANNING ELECTRON MICROSCOPY OF RUMEN DEGRADATION OF WHEAT STRAW TREATED WITH AMMONIA AND SULPHUR DIOXIDE

  • Song, Y.H.;Shimojo, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.2
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    • pp.265-270
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    • 1993
  • Ammonia and/or sulphur dioxide treated and untreated wheat leaf sheaths were compared for cell wall digestion by incubation with rumen liquor for 24 and 48 hours. Scanning electron microscope (SEM) was used to study the relative rate and extent of cell wall digestion. Treated wheat straw leaf sheaths were distorted, with more distortion observed in ammonia and sulphur dioxide combined treatment than any other treatment. Rumen liquor digestion for 24 hours of untreated leaf sheath showed disrupted phloem, partially ruptured parenchyma and vascular tissues and further partially distorted inner bundle sheaths and vascular bundle after 48 hours incubation. Sulphurated leaf sheaths showed extensive degraded parenchyma and sclerenchyma material in 24 hours incubation, however, all tissues were irregulary shaped in 48 hours incubation. In ammoniation, epidermal cell walls and small vascular bundles began to disintegrate by 24 hours incubation, extensively changed structure and degraded epidermal tissue by 48 hours incubation. Combination treatment of leaf sheaths degraded all cell walls of parenchyma, phloem and vascular bundle by 24 hours incubation, however, structures only of inner bundles sheath with extended land, sclerenchyma and cutinized epidermal cell walls remained.

Scanning electron microscopical study on the uterine development of fetuses and neonates in Korean native goats (한국재래산양의 태아 및 신생아의 자궁발달에 관한 주사전자현미경적 연구)

  • Jung, Soon-hee;Kim, Chong-sup;Kwack, Soo-dong;Lee, Jong-hwan
    • Korean Journal of Veterinary Research
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    • v.39 no.3
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    • pp.399-406
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    • 1999
  • The development of uterus in fetuses on 60, 90 and 120 days of gastation and neonates of Korean native goats was investigated by scanning electron microscopy. The results were summarized as follows ; 1. In the 60-day-old fetuses, the short microvilli were sporadically observed on the luminal surface of the endometrium. 2. In the 90-day-old fetuses, the mucosal folds, polygonal microridges, numerous microvilli, flower-like-buds, and domeshaped or crateriform area were also observed on the luminal surface of endometrium. 3. In the 120-day-old fetuses, the primordial caruncles(nodules) of the endometrium were developed conspicuously and long microvilli were developed densely. 4. In the neonates, the caruncles and microvilli of the endometrium were more developed than those of 120-day-old fetuses.

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Investigation of Domain Structure in (001) PMN-x%PT Crystals by Scanning Force Microscope (Scanning Force Microscope에 의한 (001) PMN-x%PT 단결정의 도메인 구조에 대한 연구)

  • Lee, Eun-Gu;Lee, Jae-Gab
    • Korean Journal of Materials Research
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    • v.19 no.6
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    • pp.300-304
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    • 2009
  • The domain structures of annealed (001)-oriented $Pb(Mg_{1/3}Nb_{2/3})O_3-x%PbTiO_3$ (PMN-x%PT) crystals for x = 10, 20, 30, 35, and 40 at% were investigated by Polarized Optical Microscopy (POM) and Scanning Force Microscopy (SFM) in the piezoresponse mode. Both Polar Nano-Domains (PND) and long strip-like domains were clearly observed. The results also showed how the domain structure changed between phases with an increasing x in the PMN-x%PT crystals and the domain hierarchy on various length scales ranging from 40 nm to 0.1 mm. Distorted pseudo-cubic phase (x < 20%) consisted of PNDs that did not self-assemble into macro-domain plates. The rhombohedral phase (x = 30%) consisted of PNDs that began to self-assemble into colonies along preferred {110} planes. The monoclinic phase (x = 35%) consisted of miniature polar domains on the nm scale, whereas, the tetragonal phase (x = 40%) consisted of {001} oriented lamella domains on the mm scale that had internal nano-scale heterogeneities, which self-assembled into macro-domain plates oriented along {001} the mm scale.