• 한국인터넷방송통신학회논문지
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• 제13권1호
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• pp.121-127
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• 2013

본 논문에서는 UI 시스템에서 포인팅 장비를 대신할 수 있는 컴퓨터 비전 기반의 제스쳐 형상의 영역 비율과 크기 변화 특징을 이용한 손-동작 인터페이스를 제안한다. 제안한 방법은 효과적인 손 영역 검출을 위해서 HSI 컬러 모델을 기반으로 손 영역의 피부 색상(Hue)과 채도(Saturation) 값을 혼합하여 적용함으로서 제스쳐 인식에 있어서 손 영역 이외의 피부색 영역을 제거할 수 있으며, 빛에 의한 잡음 영향을 줄이는데 효과적이다. 또한 제시되는 제스쳐의 정적인 포즈 인식이 아닌 실시간으로 변화하는 제스쳐 클리핑 영역에서의 손 영역 화소 비율과 크기 변화를 검출함으로써 계산량을 줄일 수 있으며, 보다 빠른 응답 속도를 보장한다. 제안한 컴퓨터 비전 기반의 포인팅 인터페이스는 우리가 이전 연구에서 구현한 자가 시력 측정 시스템에서 독립적인 포인팅 인터페이스로 적용한 결과, 평균적으로 86%의 제스쳐 인식률과 87%의 좌표이동 인식률을 보여 포인팅 인터페이스로의 활용도를 보였다.

• 한국토양비료학회지
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• 제33권6호
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• pp.463-471
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• 2000

본 연구는 하수오니의 농경지 이용 가능성을 밝히고자 중랑, 가좌, 나주 및 용인 하수 종말 처리장에서 채취한 하수오니를 처리한 토양에서 옥수수와 배추를 재배하여 생육 및 토양의 화학성 변화에 미치는 영향을 검토하였다. 옥수수의 생육조사결과 오니 처리율이 증가할수록 초기 생육이 저조하였다. 배추의 생육 조사 결과 초기 3주간은 12.5 및 $25Mg\;ha^{-1}$ 처리구가 좋은 생육을 보였고 후기에는 50및 $100Mg\;ha^{-1}$처리구의 생육이 더 좋은 결과를 나타내었다. 그러나 최대 생장을 보인 처리구도 화학비료 시용구에서의 생육에는 미치지 못했다. 양이온치환용량, 유기물 및 질소의 경우는 처리간의 차이는 뚜렷하였지만 재배 전후에는 큰 차이가 없었다. Na, K, Ca, Mg 등의 염기류들도 비슷한 경향을 보였다. 토양포화침출액의 EC는 재배 전과 비교하여 재배후에 감소하였고 오니처리량이 많을수록 감소폭이 커서 재배전보다 오니 처리량 간의 차이가 줄었으나 유료인산 함량은 재배중 뚜렷한 증가 경향이 나타났다. 따라서 오니시용기준은 질소가 아닌 인산을 기준으로 하여 처리량을 결정하며 부족분은 화학비료로 보충하도록 하는 것이 바람직한 것으로 사료된다.

• KSBB Journal
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• 제24권1호
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• pp.65-69
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• 2009

본 연구에서 탄소원이 고갈된 후 용존산소농도로 epothilone의 합성을 조절할 수 있음을 증명하였다. 선행연구로써, soluble starch를 탄소원으로 결정하였고, lactose와 yeast extract의 최적농도가 각각 4 g/L, 0 g/L임을 알 수 있었다. 탄소원이 고갈된 환경에서 산소농도가 낮을 경우 균체가 epothilone을 다량으로 합성하였다. 산소 공급을 조절할 수 없는 flask의 경우 배양이 진행되면서 균제농도가 증가하고 자연적으로 산소 농도도 감소하므로 epothilone이 합성되지만, 생물반응기의 경우 탄소원의 고갈 후 산소 농도를 최대한 낮게 유지시키는 것이 epothilone의 생산을 증가시켰다.

• Korean Chemical Engineering Research
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• 제61권2호
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• pp.287-295
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• 2023

Iron nanoparticles (Fe-NPs) were synthesized employing Lagenaria siceraria (LS) leaf aqueous extract as a reducing and capping medium to remove methylene blue (MB) dye and have antibacterial properties against G-negative (Escherichia coli) and G-positive bacteria (Staphylococcus aureus). The formation of LS-Fe-NPs (Lagenaria-siceraria-iron-nanoparticles) was confirmed by a change in color from pale yellow to dark brown. Characterization techniques, such as particle size analysis (PSA), transmission electron microscopy (TEM) and scanning electron microscopy (SEM), were employed to prove nano spherical particles of size range between 80-100 nm. Phytochemicals and the presence of iron in LS-Fe-NPs nanoparticles were proved by UV-visible spectrophotometry. Further, Fourier transform infrared spectroscopy (FTIR) analysis results confirmed the existence of bioactive molecules in the plants. The magnetic property was analyzed using a vibrating sample magnetometer (VSM), which displayed that the synthesized nanoparticles were superparamagnetic and exhibiting a saturation magnetization of 12.5 emu/g. Synthesized magnetic nanoparticles were used in methylene blue (MB) dye removal through adsorption. About 83% of 100 mg/L MB dye was removed within 120 min at pH 6 with a maximum adsorption capacity of 246.8 mg/g. Antibacterial efficacy of LS-Fe-NPs was screened against G-negative (Escherichia coli) and G-positive bacteria (Staphylococcus aureus), respectively, and found that LS-Fe-NPs were effective against Staphylococcus aureus.

• 제어로봇시스템학회논문지
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• 제21권12호
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• pp.1122-1129
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• 2015

This paper suggests a method of detecting traffic lights for vehicles by combining the HSV(hue saturation value) color model, morphometric characteristics, and location information appearing on consecutive images in daytime. In order to detect the traffic light, the color corresponding to the signal lights should be explored. It is difficult to detect traffic lights among colors of lights from buildings, taillight of cars, leaves, placards, etc. The proposed algorithm searches for the traffic lights from many candidates using morphometric characteristics and location information in consecutive images. The recognition process is divided into three steps. The first step is to detect candidates after converting RGB channel into HSV color model. The second step is to extract the boundaries between the housing of traffic lights and background by exploiting the assumption that the housing has lower brightness than the surrounding background. The last step is to recognize the signal light after eliminating the false candidates using morphometric characteristics and location information appearing on consecutive images. This paper demonstrates successful detection results of traffic lights from various images captured on the city roads.

• 한국수소및신에너지학회논문집
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• 제17권4호
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• pp.371-378
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• 2006

Effect of $(NH_4)_2SO_4$ precipitation and heat-treatment on hydrogenase which was extracted from the cytoplasmic fraction of the phototrophic purple sulfur bacterium Thiocapsa roseopersicina NCIB 8347 was studied. Crude enzyme extract was prepared by centrifugation($28,000{\times}g$, $400,000{\times}g$) after sonication of cells grown under photosynthetic condition for 96 hrs. Various conditions of $(NH_4)_2SO_4$ precipitation and heat-treatment were examined and the effect of protein concentration was analyzed by SDS-electrophoresis between the treatments. Optimum conditions for $(NH_4)_2SO_4$ precipitation and heat-treatment for evolution hydrogenase activity were 40-60% saturation and $60^{\circ}C$ for 20 min, respectively, which exhibited the specific hydrogenase activity of 0.78 U/mg-protein. Specific hydrogenase activity was decreased to 31.6% when the heat-treatment at $60^{\circ}C$ increased from 20 min to 5 hrs.

• 전기학회논문지
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• 제67권7호
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• pp.892-897
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• 2018

Due to population aging, an increase in the number of patients with chronic illnesses, and an increase in the number of single-person households, monitoring of health status in everyday life without the need for a hospital has become very important. For this reason, researches on various health care devices have been attempted, among which wearable devices are attracting much attention. In this paper, we propose a new ring-type wearable device for next generation healthcare. On the inner side of the ring, a metal electrodes for GSR measurement and an optical sensor for measurement of pulse wave signals of two wavelengths of red and near-infrared light were mounted. In addition, it was equipped with an acceleration sensor, and information about the degree of motion could be obtained. In this paper, it is shown that a health monitoring device can be implemented in the form of a ring, and the measured signals can be used to calculate heart rate, oxygen saturation, sleep time and sleep efficiency. Through the advanced algorithm, it is expected that we can extract various health information, especially sleep related health information by using the ring device, and it is also expected that it can contribute to the implementation of wearable healthcare effectively.

• Journal of Pharmaceutical Investigation
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• 제32권4호
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• pp.285-290
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• 2002

In order to assay the efficacy of newly synthesized antiviral compounds, in vitro studies of their active intracellular phosphorylated metabolites were established as compared with Zidovudine (ZDV). Antiviral base analogs require intracellular phosphorylation prior to the inhibition of HIV replication. Therefore, antiviral drugs concentrations in plasma have not reflected any direct relationship with activity or toxicity. A method has been developed to measure the concentration of total phosphorylated metabolites inside peripheral blood mononuclear cells using modified commercial radioimmunoassay (RIA). ZDV 5'-monophosphate was synthesized and used as a procedural control for RIA modification. PBMCs were isolated from whole blood and incubated with ZDV for 20 h to allow metabolic phosphorylation. Viable cells were extracted overnight with 60% methanol. After evaporation, the extract was reconstituted in Tris buffer. Samples were split into two fractions, one of which was treated with alkaline phosphatase (AP) to liberate phosphate groups. Concentrations of phosphorylated metabolites were determined by subtracting thε concentration of non-AP-treated fraction from that of the treated fraction. Recovery of phosphorylated ZDV from cell extracts was approximately 90%, and reproducibility was acceptable (coefficients of variation <15% for concentrations${\geq}$0.25 ng/mL). Intracellular concentrations $(0.135{\sim}5.019\;nmole/10^6\;cells)$ followed a nonlinear dose-response relationship over the range $0.015{\sim}2.996mM$ extracellular ZDV, with concentration-dependant saturation.

• 방송공학회논문지
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• 제18권4호
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• pp.550-558
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• 2013

가시광 영상과 원적외선 영상은 각각 질감 정보와 열 정보를 가지므로 서로 다른 정보를 표현한다. 그러므로 가시광 영상 개선을 위해 가시광 영상의 정보만을 이용하는 것보다 가시광 영상에서 존재하지 않는 원적외선 영상의 열 정보를 이용하는 것이 보다 좋은 결과를 얻을 수 있다. 본 논문에서는 원적외선 영상을 이용한 효과적인 가시광 영상 개선을 위해 가시광 영상에서 개선이 필요한 정도에 따라 가중치 맵을 만든다. 가중치 맵은 채도와 밝기를 이용하여 계산하며 원적외선 영상에서 열 정보를 고려하여 값을 조정한다. 마지막으로 조정된 가중치 맵을 이용하여 원적외선 영상의 정보와 가시광 영상의 정보를 융합함으로써 두 영상의 정보를 효과적으로 포함한 결과 영상을 생성한다. 실험결과에서는 가시광 영상에서 개선이 필요한 영역을 원적외선 영상 정보와의 융합으로 원본의 가시광 영상보다 향상된 결과를 보여준다.

• 동아시아식생활학회지
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• 제15권3호
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• pp.315-322
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• 2005

In this study we report on the estrogen activity of silkworm pupa and herb extracts in vitro. The estrogenic activity of these resources was investigated by competition binding assays with estrogen receptor $\alpha(ER{\alpha})\;or\;ER{\beta}$, and viability of MCF-7 cells, a human breast cancer cell line. Saturation ligand-binding analysis of $ER{\alpha}\;and\;ER{\beta}$ revealed that all plant extracts competed with estrogen ligand for binding to both ER subtypes with a similar preference and degree and competed stronger with ligand for binding to $ER{\beta}\;than\;to\;ER{\alpha}$. The highest $ER{\alpha}-binding$ sample was silkworm pupa aqueous extract The highest $ER{\beta}-binding$ sample was silkworm pupa oil. These samples were further tested for bioactivity based on their ability to regulate cell growth rate in ER(+) breast cancer cell line, MCF-7 cells. Our studies showed that silkworm pupa, soritae, sesame, yam, pueraria, malt, ginseng, Polygonum multiflorum, and Curcuma longa significantly stimulated the growth of MCF-7 cells (P<0.05). In summary, these results suggested that silkworm pupa and herbs might be useful as potential phytoestrogens.