• Archives of Pharmacal Research
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• v.2 no.2
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• pp.133-144
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• 1979

To investigate constituents of Strobilomyces floccopus (Fr.) Karst. and Coprinus comatus (Fr.) S. F. Gray, free and total amino acids of the two mushrooms were quantitatively analyzed by G. L. C. and an amino acid analyzer. Free amino acids were extracted from both mushrooms with ethanol. Fourtenn free amino acids were detected from the ethanol extract of S. floccopus and fifteen free amino acids from C. comatus by G. L. C. And the dry carphopores of both mushrooms were hydrolyzed with hydrochloric acid and then the total protein amino acids were analyzed by A. A. A. Seventeen total amino acids were detected from each acid-hydrolysate of S. floccopus and C. comatus. Lipids were extracted from the carpophores of S. floccopus and saponified with alcoholic potassium hydroxide. The isolated sterols were subjected to G. L. C. and two sterols were detected. The isolated free fatty acids were methylated with diazomethane and subjected to column chromatography and G. L. C. Eleven saturated and nine unsaturated free fatty acids were detected from the carpophores of S. floccopus. The presence of these nutrient components shows that the two mushrooms can be utilized as edible ones.

• Food Science and Biotechnology
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• v.18 no.2
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• pp.367-373
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• 2009

Regional variation in the fatty acid content of shellfish was investigated on 5 species of Korean shellfish including murex shell, ark shell, jack-knife clam, orient hard clam, and little neck clam that were originated from 2 geographically different regions in Korea (Region 1: South coast, $34-35^{\circ}N$, $127-129^{\circ}E$; Region 2: West coast, $36-38^{\circ}N$, $126-127^{\circ}E$). Significant regional difference in total fatty acids content was observed in murex shell and little neck clam (p<0.01), but not in the other species of shellfish. The contents of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids including n-3 and n-6 fatty acids were appreciably higher in murex shell from Region 2 and in little neck clam from Region 1 than the shellfish originated from their counterpart areas (p<0.05). Nevertheless, relative percentages of the fatty acids remained constant within same species regardless of geographic regions or species. Considering the facts of that the fish/shellfish are unique sources of n-3 fatty acids and a little neck clam is the most-consumed shellfish in Korea, n-3 fatty acids intake might vary with the habitat of the shellfish that Koreans consume.

• Journal of Nutrition and Health
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• v.27 no.9
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• pp.901-909
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• 1994

The effects of sardine oil(high in eicosapentaenoic acid : EPA) and tuna oil(high in docosahexaenoic acid : DHA, also high in EPA) on fatty acid composition of brain and learning ability were evaluated in male weanling Sprague-Dawley rats and compared with the effects of corn oil and beef tallow. Animals assigned by randomized block design to one of the four experimental diet groups containing dietary lipid at 15%(w/w) level were given ad libitum for 7 weeks. Food intake and body weight gain of the fish oil groups were significantly lower than those of the corn oil and beef tallow groups. However, brain weights of the groups were not significantly different. In the brain fatty acid composition, the corn oil group showed high concentrations of n-6 fatty acids, the fish oil groups of n-3 fatty acids, and the beef tallow group of saturated fatty acids. Brain EPA and DHA contents of the fish oil groups showed significantly higher than the other groups while the brain ratio of saturated/monounsaturated/polyunsaturated fatty acid was controlled in a narrow range. In a maze test, the fish oil groups appeared to arrive at the goal faster than the corn oil and beef tallow groups. It explained that EPA in diets might efficiently convert to DHA resulting in DHA accumulation in brain tissue and might increase the learning performance as DHA did.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2000.11a
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• pp.14-35
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• 2000

There have been growing interests in olive oil, which is attributed to the beneficial health effects of the Mediterranean diet. Numerous studies suggest that oleic acid, the major fatty acid in olive oil, may contribute to less incidence of coronary heart disease(CHD) in the Mediterranean area. Although the major effects of high monounsaturated fatty acid(MUFA) consumption on lowered plasma cholesterol are generally attributed to the consequential replacement of saturated fatty acid in diets, some studies have shown a modest cholesterol lowering effect of MUFA alone when it replaces dietary carbohydrate at an equal amount of energy. Furthermore, MUFA increases the level of the protective high-density lipoprotein cholesterol more than polyunsaturated fatty acid(PUFA) does when these two classes of fatty acids replace carbohydrates in the diet. It has been generally recommended that optimal dietary fat should be rich in MUFA, such as oleic acid, low in saturated fatty acids and provide adequate amounts of PUFA. However, no conclusive observations have been made yet mainly due to a difficulty in controlling the proportion of oleic acid without adjusting the levels of the other fatty acids to be constant, which could also affect plasma cholesterol levels. Recent findings also suggest that dietary PUFA results in higher tendency toward lipid peroxidation whereas with MUFA the susceptibility of low density lipoprotein to oxidative stress is reduced. More studies are needed to clarify the effects of MUFA on reducing the incidence of CHD.

• Food Science of Animal Resources
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• v.18 no.3
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• pp.261-268
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• 1998

In beef, the composition of saturated and unsaturated fatty acids(UFA) were 41.1% and 56.7%, respectively. The rates of saturated fatty acid (SFA) was 36.3, 40.9, 39.2 and 48.1% in loin, picnic shoulder, ham and rib, respectively. However, UFA showed 62.3, 58.4, 56.8, 50.9% in loin, picnic shoulder, ham and rib, respectively. The rates of monounsaturated fatty acid (MUFA) were similar to those of unsaturated fatty acid in four portions. The content of SFA, UFA and polyunsaturated fatty acid(PUFA) were 40, 59 and 9.4%, respectively, in pork. In portional comparison, composition of unsaturated fatty acids in picnic shoulder, ham rib and loin were 64.2, 60.4, 54.6 and 55.3%, respectively. The UFA contents of rib and loin were lower than picnic shoulder and ham, but SFA levels were higher. Total UFA content of chicken was 62.9% showing higher level than other species, but low when compared to that of duck meat. SFA content resulted in lower level than other portions. In the skin of chicken, MUFA content was especially high showing no significant changes in UFA contents. Duck meat showed lower SFA content, but higher content in UFA compared to those of other species. Skin and thigh contained 31.8 and 31.5%, respectively, in SFA, which are lower level than those of breast and thigh meat. The UFA contents of skin and thigh were 67.4 and 67.7%, respectively, which are relatively high compared to those of other portions. Meanwhile, the ratio of UFA was not high in canis meat compared to other meat. PUFA was, however, 24.2% showed significantly different from other meats. The contents of SFA were 35.4% and 35.9%, respectively, in picnic shoulder and ham. They showed lower SFA but higher UFA content than those of rib and loin. In contrast, the ratio of picnic shoulder and ham were 61.6 and 61.4%, respectively. Interestingly, picnic shoulder showed higher UFA content, especially in arachidonic acid level than other portions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.1
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• pp.170-179
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• 1994

Atherosclerosis, a multifactorial disease, is closely related to lipid nutrition , Data from well known epidemiological studies including Seven Country , Framinghsam Study and several intervention trials have confirmed that serum cholesterol is the major risk factor and elevation of LDL-cholesterol level is most undesriable. On the basis of results concurring in that dietary saturated fat and cholesterol increase serum cholesterol while polyunsaturated fat decrease it, changes in serum choesterol level have been predicted by regression equations developed by Keys et al. and other investigators. Effects of individual fatty acids on the level of serum cholesterol have been further differentiated by chain length, cis-trans isomers and n-6 vs n-3 polyunsaturated fatty acids. Among them the effect of n-3 fatty acids has been well recogniaed as antiplatelet activity, thus reducing the incidence of atherosclerosis. Role of vitamin E in prevention of atherosclerosis has been evovled from works showing that LDL oxdiation stimulates formation of ateroma and also from epidemiologic studies. Dietary recommendations at present are : (ⅰ) 30 and 10 cal % as upper limit of total and saturated fat intakes, respectively (ⅱ) no more than 300 mg cholesterol/day, (ⅲ) 1-2 g of n-3 fatty acid/day and (ⅳ) some increase RDA of vitamin E which is 8-10 TE.

• Journal of the Korean Applied Science and Technology
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• v.4 no.1
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• pp.83-89
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• 1987

The crude lipid contents of dried sea food products varied remarkably from 1.2 to 29.9% for dried fish products, from 2.8 to 12.3％ for dried mollusk ones, and from 0.1 to 2.3％ for dried seaweed ones. In fatty acid composition of dried fish products, the saturated acid was the most predominant component for dried anchovies, hair tail, Kstsuobushi, the monoenoic acid was the most predominant one for dried yellow corvenia, flat head, common carp, sea eel and conger eel. And the polyenoic acid was the most abundant one for dried cod. Allaska pollack, flounder, sole fish, ray, smelt and sardine. The major fatty acids of these dried fish products were 16:0, 16:1, 18:0, 18:1, 20:5 and 22:6. Fatty acid composition of dried mollusk products were mainly consisted of polyenoic acid, and followed by saturated acid, monoenoic aced. The major fatty acids of these products were similiar to those of dried fish products. And in case of dried seaweed products, saturated acid such as 14:0, 16:0 was the most predominant component, while polyenoic acid was abundant one in dried laver and sea mustard. The main fatty acids of dried seaweed products were 14:0, 16:0, 18:1, 20:1, 20:4, and 20:5. Judging from the results, dried sea food products were abundant of the highly unsaturated fatty acids in spite of the drying processing and storage.

• The Korean Journal of Mycology
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• v.6 no.1
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• pp.5-8
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• 1978

Attempts were made to investigate on the fatty acids of Agaricus bisporus, a cultivated edible mushroom. Fats were extracted from it and saponified with alcoholic potassium hydroxide. Isolated fatty acids were methylated and were subjected to column chromatography and G.L.C. Eleven saturated fatty acids, i.e., dodecanoic, tridecanoic, tetradecanoic, pentadecanoic, hexadecanoic, heptadecanoic, octadecanoic, eicosenoic, uncosenoic, docosenoic, and tricosenoic acids, were identified. Especially palmitic and stearic acids were abundant. Nine unsaturated fatty acids appear to exist in the carpophore.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.378-383
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• 2016

The objective of this study was to determine the effects of chromium methionine (Cr-Met) chelate supplementation on blood metabolites and fatty acid profile of beef from Holstein steers during late fattening period. Fifteen Holstein steers were allotted randomly into two groups including the control (non Cr-Met feeding, NCM, ave. body weight [BW] = $483{\pm}25.7kg$) and the treatment (Cr-Met feeding for 4 months, 4CM, ave. $BW=486{\pm}27.5kg$) group. The feeding amount of Cr-Met to animals was limited to 400 ppb/cow/d and was supplemented to total mixed ration. No difference in blood albumin, alkaline phosphatase, urea-nitrogen, calcium, creatine, glucose, total protein, triglyceride, and cholesterol were observed between the treatment groups (p>0.05). The level of high density lipoprotein was higher in the 4CM group than the NCM group, whereas low density lipoprotein was lower in the 4CM group (p<0.05). The fatty acid composition (caprate, laurate, myristate, pentadecanoate, palmitate, palmitoleate, margarate, cis-11 heptadodecanoate, stearate, oleate, trans-vaccenate, linoleate, cis-11 eicosenoate, docosa hexaenoic acid, and docosa pentaenoic acid) of the beef showed no difference between the two groups (p>0.05). The arachidonic acid level tended to be higher in the 4CM than the NCM group (p = 0.07). Cr-Met had no influence (p>0.05) on the ratio of saturated, unsaturated, unsaturated/saturated, monounsaturated/saturated and polyunsaturated/saturated fatty acids whereas the ratio of polyunsaturated fatty acids (PUFA) in the 4CM group was comparatively higher than the NCM group (p<0.05). This study concluded that feeding Cr-Met supplementation in 400 ppb/d to Holstein steers for 4 months during late fattening period can improve some blood metabolites and beef quality by increasing PUFA and gamma-linoleate compositions of beef.

• Nutritional Sciences
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• v.6 no.2
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• pp.78-84
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• 2003

The objective of this study was to examine the effects of seven dietary oils on the serum lipid patterns of rats. Seventy weanling Wistar Kyoto rats were divided into seven groups of ten rats each. Walnut oil (rich in PUFA), wheat germ oil (rich in PUFA), corn oil (rich in PUFA), canola oil (rich in monounsaturated fatty acids), fish oil (rich in PUFA), primrose oil (rich in PUFA), and palm oil (rich in saturated fatty acids) were employed for 21 days. Serum total cholesterol concentrations for rats fed palm oil, walnut oil, and wheat germ oil were significantly higher than were concentrations for rats receiving corn oil. fish oil, and primrose oil. The mean serum LDL cholesterol values for rats fed fish oil, primrose oil, and corn oil were significantly lower than those for rats fed walnut oil, wheat germ oil, canola oil, and palm oil. HDL cholesterol concentrations were the highest when wheat germ oil was fed and the lowest when fish oil was fed. The feeding of wheat germ oil and palm oil to rats resulted in considerably higher serum triglyceride levels than did all other treatments. The feeding of wheat germ oil to rats resulted in considerably higher serum phospholipid levels. Serum phospholipid concentrations were significantly lower in rats fed the canola oil, fish oil, ,and primrose oil diets, when compared to concentrations achieved with the feeding of walnut oil, wheat germ oil, corn oil, and palm oil. Palm oil, which has a high ratio of saturated to polyunsaturated fatty acids, resulted in the highest serum total cholesterol and highest LDL cholesterol levels, while fish oil, primrose oil, and corn oil produced the lowest total cholesterol and LDL cholesterol. Wheat germ oil produced the highest values for HDL cholesterol, triglycerides, and phospholipids. In general, feeding oils rich in polyunsaturated fatty acids produced more favorable responses than feeding oils containing large amounts of monounsaturated or saturated fatty acids.