• Title/Summary/Keyword: satellite cells

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Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells

  • Zhang, Junfang;Li, Qiang;Yan, Yan;Sun, Bin;Wang, Ying;Tang, Lin;Wang, Enze;Yu Jia;Nogoy, Kim Margarette Corpuz;Li, Xiangzi;Choi, Seong-Ho
    • Journal of Animal Science and Technology
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    • v.63 no.4
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    • pp.934-953
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    • 2021
  • Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

On-orbit Analysis of Power Generation Efficiency of Concentrating Photovoltaic System Using Commercial Fresnel Lens for Pico Satellite Applications (상용 프레넬렌즈를 이용한 극초소형 위성용 집광형 태양전력 시스템의 궤도 전력생성효율 분석)

  • Park, Tae-Yong;Chae, Bong-Geon;Oh, Hyun-Ung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.43 no.4
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    • pp.318-325
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    • 2015
  • Pico satellite has limited surface to install the solar cells due to its extremely limited size. Also, the sun incidence angle with respect to the solar panel continuously varies according to the attitude control strategy and its important parameter for the power generation. In this study, a concentrating photovoltaic system for pico satellite application has been proposed that can enhance the power generation efficiency in case of the unfavorable condition of the sun incidence angle with respect to the solar panel of the satellite using the fresnel lens. To prove the possibility of maximizing the power generation efficiency of the proposed concentrating power system, we have performed the power measurement test using a solar simulator and commercial fresnel lens. And on-orbit analysis of the power generation efficiency using the STK which is a commercial S/W has also been performed based on the test results.

In-situ and remote observation of Cochlodinium.p blooms and consequences of physical features off the Korean coast

  • Ahn Yu-Hwan;Shanmugam P.;Ryu Joo-Hyung
    • Proceedings of the KSRS Conference
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    • 2004.10a
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    • pp.553-556
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    • 2004
  • Spatial and temporal aspects of toxic dinoflagellate Cochlodinium.p blooms and consequences of physical features in complex coastal ecosystems, off the southern Korean coast, have been investigated using data obtained from SeaWiFS and AVHRR as well as in-situ observations. Hydrographic parameters measured using CTD sensors were used to elucidate physical factors affecting the spatial distribution and abundance of Cochlodinium.p blooms. The results show spatial and temporal variations of chlorophyll-a (Chl-a) and sea surface temperature (SST) and reveal significant information about Cochlodinium.p blooms and process underlying their evolution. Satellitederived Chl-a estimates appear to be potential in explicating the evolution, movement and distribution of Cochlodinium.p blooms in the enclosed bays of the South Sea. The existence of thromohaline waters offshore provide favorable conditions for the rapid growth and subsequent southward initiation of Cochlodinium.p blooms that are influenced to flow on the offshore branch (OB) during September. It was observed that there was a significant variation in the sun-induced chlorophyll-a fluorescence signal in the remote sensing fluorescence spectra and its high-intensity was recognized during the period of exponential growth and physical transport. Satellite-derived Chl-a concentration during September 1999 ranged between $3­60mg/m^3$ inside the Jin-hae and adjacent Bays and $1-6mg/m^3$ in offshore waters, with varying Cochlodinium.p abundances 1500 to 26000 cells $ml^{-1}.$ The closely spaced CTD surveys and satellite-derived SST give a complete overview on the initiation of Cochlodinium.p blooms in hydrodynamically active regions of the offshore southern East Sea by the influence of Tsushima Warm Current (TWC).

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Investigation of AI-based dual-model strategy for monitoring cyanobacterial blooms from Sentinel-3 in Korean inland waters

  • Hoang Hai Nguyen;Dalgeun Lee;Sunghwa Choi;Daeyun Shin
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.168-168
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    • 2023
  • The frequent occurrence of cyanobacterial harmful algal blooms (CHABs) in inland waters under climate change seriously damages the ecosystem and human health and is becoming a big problem in South Korea. Satellite remote sensing is suggested for effective monitoring CHABs at a larger scale of water bodies since the traditional method based on sparse in-situ networks is limited in space. However, utilizing a standalone variable of satellite reflectances in common CHABs dual-models, which relies on both chlorophyll-a (Chl-a) and phycocyanin or cyanobacteria cells (Cyano-cell), is not fully beneficial because their seasonal variation is highly impacted by surrounding meteorological and bio-environmental factors. Along with the development of Artificial Intelligence (AI), monitoring CHABs from space with analyzing the effects of environmental factors is accessible. This study aimed to investigate the potential application of AI in the dual-model strategy (Chl-a and Cyano-cell are output parameters) for monitoring seasonal dynamics of CHABs from satellites over Korean inland waters. The Sentinel-3 satellite was selected in this study due to the variety of spectral bands and its unique band (620 nm), which is sensitive to cyanobacteria. Via the AI-based feature selection, we analyzed the relationships between two output parameters and major parameters (satellite water-leaving reflectances at different spectral bands), together with auxiliary (meteorological and bio-environmental) parameters, to select the most important ones. Several AI models were then employed for modelling Chl-a and Cyano-cell concentration from those selected important parameters. Performance evaluation of the AI models and their comparison to traditional semi-analytical models were conducted to demonstrate whether AI models (using water-leaving reflectances and environmental variables) outperform traditional models (using water-leaving reflectances only) and which AI models are superior for monitoring CHABs from Sentinel-3 satellite over a Korean inland water body.

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Ultrastructural Study on the Development of the Small Granule-Containing Cells in Superior Cervical Ganglion of Human Fetus (인태아 상경신경절내 소형의 과립함유세포에 관한 전자현미경적 연구)

  • Yoon, Jae-Rhyong;Min, Young-Don;Nam, Kwang-Il
    • Applied Microscopy
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    • v.26 no.3
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    • pp.349-367
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    • 1996
  • The development of small granule-containing cell in the superior cervical ganglion was studied by electron microscopic method in human fetuses ranging from 40 mm to 260 mm crown rump length (10 to 30 weeks of gestational age). At 40 mm fetus, the superior cervical ganglion was composed of clusters of undifferentiated cells, primitive neuroblasts, and unmyelinated nerve fibers together with blood vessels. At 90 mm fetus, the superior cervical ganglion consisted of neuroblasts, satellite cell, small granule-containing cells, and unmyelinated nerve fibers. Two morphological types of the small granule-containing cells in the superior cervical ganglion were first indentified at 90 mm fetus, but were rare. Type I granule-containing cell occurred in solitary and had long processes, whereas type II cells tend to appeared in clusters near the blood capillaries. The granule-containing cells were characterized by the presence of dense-cored vesicles ranging from $150{\sim}300nm$ in diameter in both the cell bodies and processes. Other organelles included abundant mitochondria, rough endoplasmic reticulum, neurotubules, and widely distributed ribosomes. The granule-containing cells had long processes similar to those found in principal ganglionic cells. They could be identified by their content in dense-cored vesicles. The small granule-containing cells increased somewhat in size and number with increase of fetal age. Synaptic contacts were first found on the solitary granule-containing cell at 150 mm fetus. Synaptic contacts between the soma and processes of type I granule-containing cells and preganglionic axon terminals were observed. In addition, synaptic junctions between the processes of granule-containing cells and presumed dendrite of postganglionic neuron were also observed from 150 mm onward. On the basis of these features type I granule-containing cells could be considered as interneurons. The clusters of type II granule-containing cells were located in the interstitial or subcapsular portions of the ganglion, and had short processes which ended in close relation to fenestrated capillaries. Therefore it may be infer that clusters of type II granule-containing cells have an endocrine function.

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Compensation Mechanism of Cell Delay Variation by Optimum Partial Timestamps on the ATM-to-Satellite Interface (위성 TDMA 와 ATM 접속에서 최적의 부분 타임스탬프에 의한 CVD 보상 기법)

  • Chung, Ha-Jae;Kim, Jeong-Ho;Oh, Chang-Suk
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.9
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    • pp.2980-2993
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    • 2000
  • In order to achieve the rapid deployment of services. B-ISON network is being combined with terrestrial ATM and satellite network. Cell delay variation (CDV) generated by the difference of transfer mode between TOMA and ATM deteriorates transmission quality of the network system. We proposed the Partial Timestamps algorithm to supplement the problems of existing COV compensation methods. To minimize CDV and to utilize the satellite channels efficiently. only the optimized timestamps of a few cells within a control unit time of TDMA are selected and transmitted to the receiving earth station. The COV compensating efficiency of Partial Timestamps is evaluated by simulation. It is confirmed that CDV compensation capability of the proposed mechanism is superior to the other methods.

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Satellite Battery Cell Voltage Monitor System Using a Conventional Differential Amplifier (종래의 차동증폭기를 사용한 인공위성 배터리 셀 전압 감시 시스템)

  • Koo, Ja-Chun;Choi, Jae-Dong;Choi, Seong-Bong
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.33 no.2
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    • pp.113-118
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    • 2005
  • This paper shows a satellite battery cell voltage monitor system to make differential voltage measurements when one or both measurement points are beyond voltage range allowed by a conventional differential amplifier. This system is particularly useful for monitoring the individual cell voltage of series-connected cells that constitute a rechargeable satellite battery in which some cell voltages must be measured in the presence of high common mode voltage.

Voltage Balancing Circuit for Li-ion Battery System (리튬-이온 배터리 시스템을 위한 전압안정화 회로)

  • Park, Kyung Hwa;Yi, Kang Hyun
    • Journal of Korea Society of Industrial Information Systems
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    • v.18 no.5
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    • pp.73-80
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    • 2013
  • Recently, Li-ion battery is regarded as a potential energy storage device in the lime light and it can supply power to the satellite very effectively during eclipse. Because it has better features as high voltage range, large capacity and small volume than any other battery. Generally, multi cells are connected in series to use Li-ion batteries in satellite application. Since the internal resistance of cells is different each other, voltage in some cells can be overcharged or undercharged, so capacity of the cell is reduced and the life of whole battery pack is decreased. Therefore, a voltage balancing circuit with Fly-back converter is proposed and the voltage equalization of each cell is verified the prototype in this paper.

Characteristics of Infrared and Water Vapor Imagery for the Heavy Rainfall Occurred in the Korean Peninsula (한반도에서 발생하였던 집중호우 시 적외 및 수증기 영상의 특성)

  • Seong, Min-Gyu;Suh, Myoung-Seok
    • Korean Journal of Remote Sensing
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    • v.30 no.4
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    • pp.465-480
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    • 2014
  • In this study, we analyzed the spatio-temporal variations of satellite imagery for the two heavy rainfall cases (21 September, 2010, 9 August, 2011) occurred in the Korean Peninsula. In general, the possibility of strong convection can be increased when the region with plenty of moisture at the lower layer overlapped with the boundary between dark and bright area in the water vapor imagery. And the merging of convective cells caused by the difference in the moving velocities of two cells resulted in the intensification of convective activity and rainfall intensity. The rainfall intensity is more closely linked with the minimum cloud top temperature than the mean cloud top temperature. Also the spatio-temporal variations of rainfall intensity are impacted by the existence of merging processes. The merging can be predicted by the animation of satellite imagery but earlier detection of convective cells is almost impossible by using the infrared and water vapor imagery.

Resource Allocation in Two Hop Relay Network based on PB/MC-CDMA System (PB/MC-CDMA 기반 two-hop 중계 망에서의 자원 할당 방식)

  • Sun, Yan;Lee, Kye-San
    • Journal of Satellite, Information and Communications
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    • v.5 no.1
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    • pp.94-99
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    • 2010
  • In this paper, we discuss a proposed two hop relay network with PB/MC-CDMA(partial block MC-CDMA) technique. The main advantages of PB/MC-CDMA system are the increased cell capacity and the high quality of performances. In the system, we employ relay scheme to focus on the coverage reduction and hotspot problem, hotspot is a location where a cluster of users are blocked or dropped for the reason of insufficient frequency resources. Frequency resource allocation scheme is put forward in a three cells system according to each problem. The simulation result shows the performance of the two hop transmission is better than the direct transmission and the proposed network can support much more users to relief hotspot effect compared to the conventional one.