• Fisheries and Aquatic Sciences
• /
• v.26 no.6
• /
• pp.406-422
• /
• 2023

Sarcopenia is an age-related, progressive skeletal muscle disorder involving the loss of muscle mass and strength. Previous studies have shown that γ-aminobutyric acid (GABA) from fermented oysters aids in regulatory T cells (Tregs) cell expansion and function by enhancing autophagy, and concomitantly mediate muscle regeneration by modulating muscle inflammation and satellite cell function. The fermentation process of oysters not only increases the GABA content but also enhances the content of branched amino acids and free amino acids that aid the level of protein absorption and muscle strength, mass, and repair. In this study, the effect of GABA-enriched fermented sarco oyster extract (FSO) on reduced muscle mass and functions via Treg modulation and enhanced autophagy in aged mice was investigated. Results showed that FSO enhanced the expression of autophagy markers (autophagy-related gene 5 [ATG5] and GABA receptor-associated protein [GABARAP]), forkhead box protein 3 (FoxP3) expression, and levels of anti-inflammatory cytokines (interleukin [IL]-10 and transforming growth factor [TGF]-β) secreted by Tregs while reducing pro-inflammatory cytokine levels (IL-17A and interferon [IFN]-γ). Furthermore, FSO increased the expression of IL-33 and its receptor IL-1 receptor-like 1 (ST2); well-known signaling pathways that increase amphiregulin (Areg) secretion and expression of myogenesis markers (myogenic factor 5, myoblast determination protein 1, and myogenin). Muscle mass and function were also enhanced via FSO. Overall, the current study suggests that FSO increased autophagy, which enhanced Treg accumulation and function, decreased muscle inflammation, and increased satellite cell function for muscle regeneration and therefore could decrease the loss of muscle mass and function with aging.

• Animal Bioscience
• /
• v.36 no.2
• /
• pp.295-306
• /
• 2023

Objective: Inhibiting the p38 mitogen-activated protein kinase (MAPK) signaling pathway delays differentiation and increases proliferation of muscle stem cells in most species. Here, we aimed to investigate the effect of p38 inhibitor (p38i) treatment on the proliferation and differentiation of chicken muscle stem cells. Methods: Chicken muscle stem cells were collected from the muscle tissues of Hy-line Brown chicken embryos at embryonic day 18, then isolated by the preplating method. Cells were cultured for 4 days in growth medium supplemented with dimethyl sulfoxide or 1, 10, 20 μM of p38i, then subcultured for up to 4 passages. Differentiation was induced for 3 days with differentiation medium. Each treatment was replicated 3 times. Results: The proliferation and mRNA expression of paired box 7 gene and myogenic factor 5 gene, as well as the mRNA expression of myogenic differentiation marker gene myogenin were significantly higher in p38i-treated cultures than in control (p<0.05), but immunofluorescence staining and mRNA expression of myosin heavy chain (MHC) were not significantly different between the two groups. Oil red O staining of accumulated lipid droplets in differentiated cell cultures revealed a higher lipid density in p38i-treated cultures than in control; however, the expression of the adipogenic marker gene peroxisome proliferator activated receptor gamma was not significantly different between the two groups. Conclusion: p38 inhibition in chicken muscle stem cells improves cell proliferation, but the effects on myogenic differentiation and lipid accumulation require additional analysis. Further studies are needed on the chicken p38-MAPK pathway to understand the muscle and fat development mechanism.

• Korean Journal of Remote Sensing
• /
• v.33 no.4
• /
• pp.351-358
• /
• 2017

A new method to decompose the footprints of marine atmosphere boundary layer (MABL) on Synthetic Aperture Radar (SAR) imagery into characteristic spatial scales is proposed. Using two-dimensional Empirical Mode Decomposition (EMD) we obtain three Intrinsic Mode Functions (IMFs), which mainly present longitudinal rolls, three-dimensional cells and atmospheric gravity waves (AGW). The rolls and cells have spatial scales between 3.0 km and 3.8 km and between 5.3 km and 7.1 km, respectively. Based on previous observations and mixed-layer similarity theory, we estimated MABL's depths that vary from 0.95 km to 1.2 km over the rolls and from 3.0 km to 3.8 km over the cells. The AGW has maximum spectrum at 14.3 km wavelength. The method developed in this work can be used to decompose other satellite imageries into individual features through characteristic spatial scales.

• Proceedings of the KSRS Conference
• /
• v.1
• /
• pp.220-223
• /
• 2006

The COMS(Communication, Ocean and Meteorological Satellite) EPS(Electrical Power Subsystem) is derived from an enhanced Eurostar 3000 EPS which is fully autonomous operation in normal conditions or in the event of a failure and provides a high level of reconfiguration capability and flexibility. This paper introduces the COMS EPS preliminary design result. The COMS EPS consists of a battery, a solar array wing, a PSR(Power Supply Regulator), a PRU(Pyrotechnic Unit), a SADM(Solar Array Drive Mechanism) and relay and fuse brackets. This can offer a bus power capability of 3 kW. The solar array is made of a deployable wing with two panels. One type of solar cells is selected as GaAs/Ge triple junction cells. Li-ion battery is base lined with ten series cell module of five cells in parallel. PSR associated with battery and solar array generates a power bus fully regulated 50 V. Power bus is centralised protection and distribution by relay and fuse brackets. PRU provides power for firing actuators devices. The solar array wing is routed by the SADM under control of the AOCS(Attitude Orbit Control Subsystem). The control and monitoring of the EPS especially of the battery, is performed by the PSR in combination with on-board software.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.38 no.5
• /
• pp.453-463
• /
• 2001

This paper presents an absolute position estimation method using Indian remote sensing (IRS) satellite images, which is a part of a position estimation (PE) system. The accumulated buffer (AB) matching method is proposed, in which a set of accumulator cells is employed for fast edge-based matching. By computer simulations with two sets of veal aerial image sequences, the performance of the AB matching method is analyzed and its effectiveness is shown in terms of the position error in the hybrid PE system.

• Aerospace Engineering and Technology
• /
• v.1 no.1
• /
• pp.84-96
• /
• 2002

The major goal of this research is to use as a baseline guide for a flight model design of power system of next domestic communication satellite. For this purpose, the EPS(Electrical Power Subsystem) is designed to compliance performance requirements specified in EPS subsystem specification during all expected spacecraft operations. The regulated electrical power bus gives 42.5V to the various spacecraft loads from PCDU(Power Control & Distribution Unit) and the solar arrays are composed of 6 panel, each panel has 3 circuits including 7 string. The battery system is comprised of two batteries consisting of 26 IPV(Individual-Pressure-Vessel) NiH2 cells. Each battery can be capable of delivering 2878Watt-hours at a 80% maximum DOD(Depth of Discharge) based on the nameplate capacity of 150 amper-hours.

• Journal of information and communication convergence engineering
• /
• v.7 no.4
• /
• pp.580-585
• /
• 2009

Harmful Algal Blooms (HABs), caused by Cochlodinium polykrikoides that causative fishery mortality, impact on aquaculture and economic loss appear particularly in summer and fall seasons in the Korean seas. It was studied on characteristics of HABs in the South Sea of Korea by using satellite and in-situ data. The in-situ data encompassed oceanic and meteorological data from July to October 2002-2008 and satellite data from July to October 2002-2006. Chlorophyll concentrations were calculated using Seaviewing Wide Field-of-view Sensor images by an Ocean Color (OC4) algorithm, and HABs were estimated using the Red tide index Chlorophyll Algorithm (RCA). The HAB occurrences were dominant when water temperature was $22.6-28^{\circ}C$ in August. The frequency of the individual numbers during 2002-2008, the HABs more than 1000 cells/ml (alert condition), were 73.57 %. In meteorological data from July to September during 2002-2008, the average precipitation, the mean air temperature, the mean wind speed and direction, and the sunshine were 9.31 mm/day, $24.07^{\circ}C$, 2.34 m/s and easterly, and 1-11 h, respectively. Our results suggest that the upwelling is caused by southwesterly wind in summer season and the Tsushima Warm Current which have influenced on the dispersion and moving of HAB (chlorophyll). In addition, the fresh water from Nakdong River, as the source of nutrients, also influences the occurrence of HABs.

• Journal of Advanced Navigation Technology
• /
• v.23 no.6
• /
• pp.507-514
• /
• 2019

Federal communications commissions (FCC) has announced the first set of rules for 3.5 GHz (3,550 - 3,700 MHz) band used Navy radar and fixed satellite service (FSS) earth stations as primary service to sharing for citizen broadband radio service (CBRS) enable development of relatively low powered network technologies like small cells. Since CBRS sharing technique based on the 3-tiered spectrum access system (SAS) is able to protect the higher tiered users from harmful interference from lower tiered users, it has been considered actively to be introduced in Korea. However, 3.5 GHz band had been already allocated to the 5G services in Korea, the sharing studies have been carried out for 3,700 - 5,000 MHz, As the result, the 3.8 GHz (3,800 - 3,900 MHz) band used for only FSS system is able to be sharing, and an introduction of CBRS has been proposed in Korea by analyzing the interference to the FSS earth stations.

• Animal cells and systems
• /
• v.15 no.1
• /
• pp.37-43
• /
• 2011

The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight micro satellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These micro satellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

• Molecules and Cells
• /
• v.22 no.3
• /
• pp.300-307
• /
• 2006

Brassica rapa ssp. pekinensis (Chinese cabbage) is an economically important crop and a model plant for studies on polyploidization and phenotypic evolution. To gain an insight into the structure of the B. rapa genome we analyzed 12,017 BAC-end sequences for the presence of transposable elements (TEs), SSRs, centromeric satellite repeats and genes, and similarity to the closely related genome of Arabidopsis thaliana. TEs were estimated to occupy 14% of the genome, with 12.3% of the genome represented by retrotransposons. It was estimated that the B. rapa genome contains 43,000 genes, 1.6 times greater than the genome of A. thaliana. A number of centromeric satellite sequences, representing variations of a 176-bp consensus sequence, were identified. This sequence has undergone rapid evolution within the B. rapa genome and has diverged among the related species of Brassicaceae. A study of SSRs demonstrated a non-random distribution with a greater abundance within predicted intergenic regions. Our results provide an initial characterization of the genome of B. rapa and provide the basis for detailed analysis through whole-genome sequencing.