• Mycobiology
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• 제36권2호
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• pp.106-109
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• 2008

The anti-tumor effects of exo- (EX) and endo-biopolymers (EN) produced from submerged mycelial cultures of Ganoderma applanatum (GA), Collybia confluens (CC), and Pleurotus eryngii (PE) were studied using Sarcoma 180 bearing mice. Solid tumor growth was inhibited most effectively when 40 mg/kg body weight (BW) of GA-EX or PE-EN was administered to the intraperitoneal (i.p.) cavity of BALB/c mice. The spleen and liver indexes were increased in mice following i.p. administration of GA-EX and PE-EN fractions. GA-EX and PE-EN reduced the tumor formation by 30.7% and 29.4%, respectively. GA-EX and PE-EN increased the natural killer (NK) cell activity of splenocytes by 41.3% and 28.9%, respectively.

• 약학회지
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• 제17권2호
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• pp.115-121
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• 1973

Seven ${\beta}$-substituted-2,4-dihydroxy-5-nitropropiophenones were synthesized as potential antineoplastic agents, and subjected to the screening test of survival effect and cell decrease effect. Of the sunthesized compounds, ${\beta}$-(1-methyl-1-hydroxymlethy) ethylamino-2, 4-dihydrocy-5-nitropropiophenone and ${\beta}$(1,1-dihydorxymethyl) ethylamino-2,4-dihydroxy-5-dihyropropiophenone and ${\beta}$-(1,1-dihydroxymethyl) propylamino-2,4-dihydroxy-5-nitropropiophenone were found to be active against both Ehrlich ascites carcinoma and Sarcoma 180.

• 생약학회지
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• 제31권1호
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• pp.16-22
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• 2000

These studies were designed to determine the potential cytotoxic activity of MeOH extracts of 65 crude drugs against leukemia L1210 and $P388D_1$, cell line in vitro, of which 25 samples were selected. The n-BuOH extracts of 25 samples were measured using the same method and nine were selected. These samples were measured for the potential antitumor activity against $P388D_1$, for life span in vivo, and against sarcoma 180 for tumor weight. On the basis of results, Notoginseng Radix, Anemarrhenae Rhizoma, Albizziae Cortex, Portulacae Herba, Bupleuri Radix were evaluated the effective plant on the antitumor activity. In addition, the mixture of Notoginseng Radix and Albizziae Cortex was evaluated to enhance the antitumor activity in vivo.

• 식품기술
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• 제12권3호
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• pp.8-13
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• 1999

키틴/키토산은 지질흡수의 억제, 혈중 콜레스테롤의 저하, 항고혈압활성, 면역활성, 항종양/항암활성 등 다양한 생체기능성을 나타내어 건강지향적인 기능성 식품으로서의 이용가치가 매우 큰것으로 평가되고 있다. 키토산은 성인남자의 혈중 콜레스테롤을 감소시키고 HDL cholesterol은 증가시켜 동맥경화지수를 낮추며 비만환자에게 투여시 체중, 중성지질, LDL cholesterol을 유의하게 낮춤으로서 고지혈증과 비만증의 개선에 유용한 것으로 평가된다. 키토산은 또한 성인의 고염식에 의한 혈압상승을 억제하며 3량체 내외의 키틴/키토산 올리고당은 혈압상승의 중요인자인 angiotensin converting enzyme과 직접 반응하여 활성을 현저히 저하시키고 SHR에서의 혈압을 유의하게 억제하는 특성을 보여 고혈압의 억제 및 치료에도 응용가치가 클 것으로 생각된다. 키틴/키토산 및 그 올리고당은 sarcoma 180, Meth-A solid tumor의 성장을 저해하고 L1210와 같은 negative charge를 갖는 malignant cell을 흡착시키는 등 항종양/항암활성을 보이는데 이는 tumoricidal immunocite의 활성화에 의한 것으로 추정되고 있다. 키틴/키토산의 생체활성은 분자크기, 탈아세틸화도, 유도체의 종류 및 적용방법 등에 따라 차이를 보이기 때문에 키틴/키토산을 기능성 식품으로서 폭넓게 이용하게 위해서는 용도에 맞는 적절한 규격 설정이 요구되고 있다.

• Preventive Nutrition and Food Science
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• 제4권2호
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• pp.117-121
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• 1999

The anititumor and immunologic activities of the glycoproteins extracted from sea cucumber (Stichopus japonicus) on mice bearing sarcoma 180 cells were investigated . Maximum tumor suppression (64%) occurred at the dose of 100mg glycoprotein/kg. The highest prolongation ratio was achieved at the level of 100mg/kg an dincreased by 395 more than that of control. Glycoproteins from sea cucumber exhibited direct cytotoxic effect on the tumor cells. Dose dependent increase of leucocyte, peritoneal exudate cell and weights of immunoorgans revealed the improvement of immunity. When the glycoportein-administered group was compared with the control, a significant difference was not noted in the clinico-chemical values such as S-GOT, S-GPT , alkaline phoshatse activity, total protein, cholesterol, triglyceride, urea nitrogen and glucose levels in blood. These results suggests that the antitumor activity of sea cucumber glycoprotein is associated with activation of cells in the immune system.

• Journal of Ginseng Research
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• 제15권2호
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• pp.99-105
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• 1991

This experiment was performed to investigate the effects of ginseng saponin fraction and cyclophosphamide (CY) on the tumor development, the antitumor effect and the tumoricidal activity of mouse macrophage. When mice were treated with saponin or CY following inoculation with Sarcoma 180, tumor development was inhibited and survival ratio increased, and a combination of both treatments further inhibited the tumor development. Tumoricidal activity of macrophage was effectively increased at 10-7% concentration of CY and it was further increased when macrophage was cotreated with saponin and CY. Tumoricidal activity of macrophage was greatest at the third day after inoculating tumor cell. Both saponin and CY increased the chemiluminescence of macrophage, but CY had no effect on releasing TNF, unlike saponin.

• 한방안이비인후피부과학회지
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• 제18권1호
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• pp.71-81
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• 2005

Objective : This Study was to investigate effects of Takli-San on the anti-cancer and proliferation of immunocytes, nitric oxide(NO) production of peritoneal macrophages. Methods : We used Takli-San extract(TLS) with freeze-dried, 8wks-old male mice and cancer cell lines(L120, Sarcoma-180) for this Study. The cytotoxicity and proliferation of cells were tested using a colorimetric tetrazoliun assay(MTT assay). Results : 1. TLS was significantly showed cytotoxicity on the L1210 cell lines. 2. TLS was significantly increased proliferation of thymocytes and splenocytes in vitro. 3. TLS was significantly increased proliferation of thymocytes by all-dosage, but proliferation of splenocytes by low-dosage in normal mice. 4. TLS was significantly increased NO production from peritoneal macrophages in normal mice. 5. TLS was significantly decreased proliferation of L1210 cells in L1210 cells transplanted mice. 6. TLS was significantly increase proliferation of thymocytes by all-dosage, but proliferation of splenocytes by low-dosage in L1210 cells transplanted mice. 7. TLS was significantly increased NO production from peritoneal macrophages in L1210 cells transplanted mice. Conclusions : The present author thought that TLS had action of anti-cancer by becoming immunocytes activity(NO production, proliferation of thymocytes).

• 대한한의학회지
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• 제22권2호
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• pp.22-30
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• 2001

This study was attempted to investigate the anti-tumor and anti-metastatic effects of Sobokchukeotang(SBCT) and Kamisobokchukeotang(KSBCT). Cytotoxicity against various cancer cell lines, anti-adhesion, pulmonary colonization, anti-angiogenesis, and T/C% were evaluated. SBCT and KSBCT exhibited no cytotoxicity against HT-1080, A549, SK-OV-3, B16-F10 and SK-Mel-2 cell lines. In inhibitory effect on DNA topoisomerase I, the $IC_{50S}$ were shown $250-500{\;}\mu\textrm{g}/ml$ of SBCT and $62.5-125{\;}\mu\textrm{g}/ml$ of KSBCT respectively. In the in vivo experiments, SBCT(135.98%) and KSBCT(151.92%) apparently increased the life span of mice bearing sarcoma-180. KSBCT significantly inhibited the adhesion of HT-1080 to complex extracellular matrix in a dose-dependent manner in contrast to SBCT. In pulmonary colonization assay by B16-F10, a number of colonies in the lungs were decreased more significantly in KSBCT group than those in SBCT group. In vitro neovascularization and CAM assay, angiogenesis was more significantly inhibited in KSBCT-treated group than in SBCT- treated group. Above results suggests that KSBCT is more effectively applied to prevention and treatment of cancer than SBCT.

• Macromolecular Research
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• 제9권5호
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• pp.277-284
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• 2001

Poly(exo-3,6-epoxy-1,2,3,6-tetrahydrophthalic anhydride)s [poly(ETA)s] and poly($\alpha$-ethoxy-exo-3,6-epoxy-1,2,3,6-tetrahydrophthaloyl-5-fluorouracil)s [poly(EETFU)s ] with various average molecular weights were prepared by photopolymerizations. The number average molecular weights of the fractionated poly(ETA)s and poly(EETFU)s determined by GPC were in the range of 3,600∼21,000 and 3,600-33,400, respectively. The release rate of 5-FU from poly(EETFU) decreased with increasing average molecular weight. The in vitro cytotoxicity of poly(ETA) against a normal cell line was lower than that of 5-fluorouracil(5-FU), The in vivo antitumor activities of the synthesized samples at dosage of 0.8 mg/kg against mice bearing sarcoma 180 tumor cell line decreased in the following order: poly(EETFU) > poly(ETA) > EETFU > ETA > 5-FU. The antiangiogenic activities of the poly(ETA)s were better than those of 5-FU.

• 대한한방내과학회지
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• 제28권2호
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• pp.321-332
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• 2007

Objectives : Anticancer and immune-modulating effects of several Korean medical prescriptions including Yukgunja-tang, Bohwa-tang, Sogam-Won, and Kamiboa-tang were investigated. Methods : In vitro anti-cancer effects were measured by cytotoxicity MTT assay using SNU-1 gastric cancer cell lines, In vivo anti-cancer effects were measured by increased life span of S-180 sarcoma-injected ICR mouse. Immune-modulating effects were analyzed by measuring hemagglutinin titer, appearance of rosette forming cells, lymphocyte proliferation, and phagocytic index in methotrexate-pretreated mice. Results : In vitro assay showed that only Sogam-won showed cytotoxic effect with $IC_{50}$ of 87.9 ${\mu}g/ml$. All other prescriptions showed no cytotoxic effects against SNU-1 gastric cancer cell line. However, in vivo assay showed that Sogam-won showed lowest anti-cancer effects in contrast to its highest cytotoxic effects, Kamiboa-tang, which showed no cytotoxic effect, showed the highest in vivo anticancer effects, with increased life span of 140%. Kamiboa-tang showed significant immune-enhancing activities by significantly increasing rosette forming cells, lymphocyte proliferation, and phagocytic index in methotrexate-pretreated mice (P<0.05). Conclusion : The anticancer effect of Kamiboa-tang is not mediated by direct inhibition of cancer cells but is mediated by improving immune reactions against cancer cells.