• Journal of the Korean Wood Science and Technology
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• v.44 no.6
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• pp.872-879
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• 2016

Demand of natural interior finishing material has been widely sprayed in nowadays because many weak people as children, pregnant women, and elder people are being struggled with sick house syndrome due to volatile organic compounds such as formaldehyde, toluene, benzene, etc. Our research group developed a no-added formaldehyde adhesive for wood-based panels from mainly rice powder and some additives in the previous study for abating sick house syndrome. Since the rice powder adhesive provides a good source of nutrients with microorganisms, it was suspected a susceptibility of the rice powder adhesive to fungal and sapstain attack. We evaluated anti-sapstain activity of the rice powder adhesives modified by adding wood preservatives. We modified the rice powder adhesive by adding three different types of anti-sapstain preservatives at three different concentrations to assess their anti-sapstin activity. The bonding strengths of the modified rice powder adhesives were still outstanding performance on all samples. Moreover, the plywood manufactured with the modified rice powder adhesive satisfied outdoor use requirement for ordinary plywood (KS F3101, Korean Standard). The results obtained showed that at least 3% of preservative should be added to the rice powder adhesive to obtain effective anti-sapstain activity.

• Journal of Korea Foresty Energy
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• v.22 no.2
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• pp.36-43
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• 2003

This study was carried out to evaluate the fungicidal, anti-sapstain and termiticidal efficacy of commercial Dan-Chung which are currently being used for painting to decorate and to provide durability with structures of temples. Two binders (Polysol and Gelatine), and eleven pigments (Iron Oxide Red, Lead Red, Toluidine Red, Chrome Yellow, Titanium Dioxide, Ultramarine Blue, Chrome Oxide Green, Permanent Black, Cyanine Green and Ho-bun(Chalk)) were applied for the evaluation. The fungicidal and anti-sapstain activity of each pigment were depended on the types of binders. When the Polysol was used as a binder, the fungicidal efficacy of Toluidine Red treated specimen was close to the standard value (above 80) with grater than 70 against to two wood decay fungi (T. palustris and T. versicolor). The anti-sapstain efficacy of Lead Red treated specimens was excellent against to three sapstain fungi (P. funiculosum, T. viride and R. nigricans), and that of Ultramarine Blue treated specimens was also excellent to T. viride. The other pigments had little efficacy to these micro-organisms. Although there were some different trends of the effectiveness depending on the types of binders, the anti-termite effectiveness of Chrome Oxide Green and Chrome Yellow was outstanding to termite tests.

• Journal of the Korean Wood Science and Technology
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• v.23 no.1
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• pp.42-48
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• 1995

The purpose of this study is to evaluate the efficacy of metabolites produced form Streptomyces rimosus in controlling the growth of sapwood - inhabiting fungi. In order to carry out this task, the following specific fungi were tested : sapstain fungi - Ceratocystis pilifera, Ceratocystis piceae, and Aureobasidium pullulans ; mold fungi - Trichoderma hazianum, Trichoderma viride, Penicillium cirtrinum, and Aspergillus niger. Based on the tests, the following observations can be drawn. 1. The conidial germination of sapstain and mold fungi was completely inhibited leaving a clear zone around the paper disc treated with metabolites. The best inhibition was observed in A. pullulans plate and the least in T. viride. 2. Concentration of SB medium for the production of metabolites from St, rimosus affected antifungal activity of metabolites against sapwood - inhabiting fungi. Metabolites prepared from 1/3${\times}$SB medium showed the best activity and the least activity was observed in metabolites form 1/4${\times}$medium. 3. in vivo and in vitro test using wood blocks, treatment of pine sapwood blocks with metabolites also inhibited conidial germination and thus prevented discoloration. 4. Treatment with metabolites did not change the macroscopic structure of wood and did not cause the discoloration of the surface of wood by pigments produced form St. rimosus. In conclusion the results of this study indicate that antifungal metabloites of St, rimosus could be used for the biological control of sapstain and mold fungi.

• The Plant Pathology Journal
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• v.21 no.1
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• pp.1-6
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• 2005

During last decade there has been noticeable progress in the research of the biology of sapstaining fungi that cause considerable economic losses to forest product industry. The researches generated broad ranges of knowledge on sapstaining fungi regarding their occurrence on conifer wood, taxonomy, nutrient physiology, pigmentation biochemistry and molecular biology, and biological control. Major problematic groups in the sapstain production are Ophiostoma, Ceratocystis, and Leptographium genera. With Ophiostoma as a model, it is found that the type of carbon source is important in the growth and pigment production of sapstaining fungi. The operation of dihydroxy naphthalene (DHN) melanin pathway for black to bluish pigment production has been confirmed in those cosmetic fungi both at biochemical and molecular levels. The development of albino technology using nutrition competition has been shown to be promising as an environmentally friendly biological control method for sapstain control.

• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.559-565
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• 2013

This study evaluated the ability of white and brown rot fungi to decompose fire retardant-treated wood by measuring mass loss. Anti efficacy of FRT against sapstain and mold fungi was evaluated. Wood was treated with liquid sodium silicate and boric acid, ammonium borate, di-ammonium phosphate. Retardant treated wood was then subjected to fungal decay resistance tests performed according to KS standard method using a brown-rot fungus, Fomitopsis palustris and white rot fungus Trametes versicolor. Aspergillus niger, Penicillium funiculosum, Rhizopus nigricans, Aureobasidium pullulans, Tricoderma virede fungi were used anti-sapstain and mold test. Boron and phosphorus chemicals used in this study increased the resistance of fire retardant treated wood against both fungal attack. Anti mold and sapstain efficacy of the fire retardant treated wood was excellent but there were difference depend on mold. After the liquid sodium silicate treatment, the second chemical treatment process could lead chemical fixation into wood, which effects decay resistance.

• Mycobiology
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• v.30 no.1
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• pp.37-40
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• 2002

The susceptibility of radiata pine sapwood to fungal attack and the ability of selected fungicides to control colonization of sapstain and mold fungi on green radiata pine sapwood were evaluated. Radiata pine sapwood was highly susceptible to fungal staining, suggesting that prompt application of fungicides after sawing is essential for preventing fungal colonization. The ability of commercial fungicides to prevent fungal discoloration on radiata pine sapwood was assessed using an accelerated 6-week test on small samples in the laboratory, and in field tests using bulk-piled boards exposed outdoors for 6 weeks during summer rainy season. In laboratory tests, Hylite extra provided excellent protection against fungal discoloration even at the lowest concentrations. Hylite clear, Britewood S, and NP-1 Plus provided good short-term protection(2 to 4 weeks), but higher chemical loadings were, required for long-term protection(6 weeks). Woodguard produced little or no protection over the test periods. In field tests, Kathon 893 provided markedly superior protection at the concentration of 0.5 percent or higher. NP-1 Plus provided relatively good protection at all concentrations evaluated. Hylite extra was effective only for short-term protection(2 to 4 weeks) at all concentrations tested, but higher solution strengths were needed for longterm protection.

• Journal of the Korean Wood Science and Technology
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• v.30 no.2
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• pp.128-133
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• 2002

This study was conducted to investigate the influence of storage time on the sapstain development of Japanese red pine and Korean pine logs during storage in log yard, and their stain characteristics. Japanese red pine and Korean pine trees were harvested and cut into logs in mid January of 2001. These logs were transported to the two local sawmills where they were closely stacked in remote parts of log yard. The logs were then sampled destructively by cutting seven to nine 3-cm long discs along the length of each log at intervals of 3, 4, 5, 6, and 8 month after felling. The stain coverage and maximal radial penetration of stain were measured from the discs of the sampled logs after the isolation of causal staining fungi. The sapstain was primarily infested by the attack of bark beetles and the species of bark beetle was identified as Tomicus piniperda. The main fungal species isolated from stained wood was Ophiostoma species. Based on the present study, the logs could be stored in log yard until May without stain; but stain development was rapid after May and the severity of stain increased proportionally with storage time. Korean pine was more susceptible to fungal stain than Japanese red pine. During summer storage, decay started to develop in logs and the main species were identified as Tyromyces sp. and Schizophyllum commune. Information provided in this paper would be very useful to develop more effective control strategies for sapstain prevention in Japanese red pine and Korean pine logs.

• Journal of the Korean Wood Science and Technology
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• v.49 no.6
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• pp.591-601
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• 2021

Recently it is trend to increase wood use as carbon neutral materials, there is recognized to need necessarily durability improvement of wooden building. It is very rare to report existing on the identification of isolates causing discoloration in domestic weathered wooden building used for long period. The objective of this study was identification of fungi that cause discoloration on the exteriors of weathered domestic wooden buildings in the southern part of South Korea. Our findings can be helpful to establish protection technology for weather deterioration of domestic wooden buildings. Wood chip samples presumed to be contaminated with sapstain fungi were collected from the surface of wooden members used in wooden buildings at Jangheung, Jeollanam-do (two locations, #13 and #14), and Jeju Island (two locations, #31 and #33). The growth of microorganisms was confirmed by performing culture tests for the collected samples, and fungi were isolated, purified, and identified. The results indicated that the fungal strains isolated from wooden buildings #13 and #14 at Jangheung, Jeollanam-do, were 99.83% and 100% homologous to Aureobasidium melanogenum, respectively. For wooden building #31 at Jeju Island (two locations), the fungal strain isolated was 100% homologous to A. melanogenum, which is the same species isolated from the wooden buildings at Jangheung. The fungal strain isolated from wooden building #33 (Jeju Island) had 99.83% homology with A. pullulans, which is commonly found in wood degraded by weather or ultraviolet rays. Our findings can be utilized as a basis for establishing protection technology in domestic wooden buildings.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.71.2-72
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• 2005

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.41-41
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• 2005