• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.739-749
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• 2013

The seasonal and spatial diversity of picocyanobacteria (Pcy) in lakes of the Great Mazurian Lakes (GLM) system was examined by DGGE analysis of molecular markers derived from the 16S-23S internal transcribed spacer (ITS) of the ribosomal operon and the phycocyanin operon (cpcBA-IGS). The study of nine lakes, ranging from mesotrophy to hypereutrophy, demonstrated seasonal variance of Pcy. The richness and Shannon diversity index calculated on the basis of both markers were higher in spring and lower in early and late summer. No statistically significant relationships were found between the markers and trophic status of the studied lakes or Pcy abundance. There were, however, statistically significant relationships between the diversity indices and sampling time. The analysis pointed to a different distribution of the two markers. The ITS marker exhibited more unique sequences in time and space, whereas a greater role for common and ubiquitous sequences was indicated by the cpcBA-IGS data. Examination of the Pcy community structure demonstrated that communities were grouped in highly similar clusters according to sampling season/time rather than to the trophic status of the lake. Our results suggest that time is more important than trophic status in shaping the diversity and structure of Pcy communities. The seasonal changes in picocyanobacteria and differences in diversity and community structures are discussed in the context of well-established ecological hypotheses: the PEG model, intermediate disturbance hypothesis (IDH), and horizontal gene transfer (HGT).

• Journal of the Korean Geosynthetics Society
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• v.17 no.3
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• pp.9-18
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• 2018

The soil parameters important for the design of the soft ground are the compression index ($C_c$), the consolidation settlement and consolidation speed at the field. Compression index is obtained by laboratory consolidation test. In the laboratory consolidation test, sample disturbance always occurs. In order to correct the disturbance phenomena, the method of calculating the compression index proposed by Schmertmann (1955) is generally used. However, recent developments in sampling technology and Korean soil conditions are different from those proposed by Schmertmann. So it needs to be verified. In this study, each consolidation curve's cross void ratio is evaluated by doing consolidation test varying disturbance on high-plastic clay (CH), low-plastic clay (CL) and low-plastic silt (ML). The test results were $0.521e_0$ for low-plastic silt, $0.404e_0$ for low-plastic clay, and $0.458e_0$ for the high-plastic clay. This results were different from those of Schmertmann's suggested value of $0.42e_0$. Therefor we proposed a correction formula using the plastic index according to soil type. However, since the results of this study are limited test results, further studies on various korean soil are needed to suggest the compression index correction method according to the degree of plasticity index of soil.

• Journal of the Korean Geotechnical Society
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• v.24 no.2
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• pp.77-86
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• 2008

The disturbance zone and measured values are affected by the size of the penetrometer. The local value may be measured by the smaller penetrometer. An ultra small Micro-Cone penetrometer (5mm in outer diameter) is designed and manufactured to characterize soil properties with minimum disturbance during penetration tests. The tip resistance is measured by using stain gauges attached near the Micro-Cone. In addition, the friction sleeve is adopted to effectively remove the skin friction from the tip resistance. Design concern includes the installation of stain gauges, circuits, penetration systems, penetration rate, sampling rate, operating temperature, and calibration. Application tests show that the clay interface, and the soil layers consisting of clay and sand are clearly detected by the Micro-Cone. Furthermore, the cone tip resistances measured by the Micro-Cone and the miniature cone (16mm in outer diameter) are similar. Note the resolution is much higher in the Micro-Cone. This study shows that the Micro-Cone may effectively detect the soil interface with high resolution, and with minimum disturbance.

• Journal of the Korean Geotechnical Society
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• v.21 no.3
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• pp.141-148
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• 2005

The settlement measured in the field shows a considerable difference from the predicted settlement due to various factors such as nonhomogeneous soft layers, sampling disturbance, erroneous selection of soil parameters and deficiency of consolidation theories among others. Therefore, analysis of actual settlement behavior based on the instrumentation and measurement data during consolidation period is a very useful procedure in evaluating the rates of consolidation settlement. This paper introduces a new practical method of analyzing consolidation settlement behavior, which is generalized and formulated from the existing analysis methods such as Hyperbolic method, Hoshino method and $\sqr{s}$ method. Through case studies, it is shown that the proposed method is a superior technique in reflecting the measured settlement behavior.

• Journal of Ecology and Environment
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• v.32 no.3
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• pp.197-206
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• 2009

Benthic macroinvertebrate communities were collected from six different sites in the Dobong Stream in Seoul, Korea to investigate spatial and temporal changes in benthic macroinvertebrate communities in response to natural disturbances such as floods and droughts. We collected samples monthly or semimonthly with a Surber net ($30cm{\times}30cm$), and measured environmental factors, including stream temperature, discharge, width, conductivity, dissolved oxygen and pH at each sampling site. Benthic macro invertebrates were strongly affected by floods as well as droughts. In addition, benthic macroinvertebrate communities displayed different responses to the onset of the rainy season in summer 2006 and 2007, apparently due to differences in the intensity and amount of precipitation. Chironomids were particularly sensitive to heavy rain. Floods and droughts also affected the proportions of functional feeding groups during the survey period: the proportion of scrapers was high right after heavy rains, while the proportion of predators tended to increase in intermittent-type streams as the riffle zone decreased. Finally, although species richness and abundance were strongly influenced by heavy rain, they recovered to background levels for within one month, and varied consistently among stream types, indicating habitat stability.

• Journal of Advanced Marine Engineering and Technology
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• v.15 no.1
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• pp.85-95
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• 1991

All physical systems are nonlinear to some degree. The examples are relay, backlash, deadzone, saturation element and so on. In the linear control system design, it is useful method to restrict the nonlinearity to the linearity of system over the operation range. It is worth noting that nonlinearities may be intentionally introduced in to a system. A simple of an intentional non-linearity is the Bang-Bang controller which uses the On-Off relay. In this paper, an angular position servosystem made of a DC servomotor controlled by a microcomputer is discribed. Authors use two methods in the design of controller. The one is linear controller designed by the optimal feedback control theory only and the other is nonlinear controller designed by On-Off relay with optimal feedback control theory. To do the real time control, the controller is designed by using 16bit personal computer and A/D.D/A converter(12bit) is used in order to convert the signal. According to this way, the results from real time control are as follows. 2) Under the On-Off controller with hysterisis the influence of disturbance is considerably smaller than the linerar controller. 3) An increase in the sampling period has a destabilizing effect. 4)In the controller performance, the response time of the On-Off controller is longer than that of the linear controller. To close, we note that the On-Off controller with hysterisis is more attractive than the linear controller in the presence of the input limit.

• ALGAE
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• v.26 no.1
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• pp.61-71
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• 2011

Thirty populations of Chondrus crispus Stackhouse from Nova Scotia were collected during the years 1993 to 2011. Taken from estuaries, wave exposed open coasts, high intertidal rock pools and shallow subtidal habitats, the populations were evaluated for relative abundance of tetrasporophytic and gametophytic life history phases. Over 2,800 thalli were characterized using the resorcinol-acetal test to distinguish the kappa- and lambda-carrageenan containing fronds of gametophytes and tetrasporophytes, respectively. These populations had $77{\pm}5%$ gametophytes (mean ${\pm}95%$ confidence interval), with most populations having gametophyte : sporophyte ratios ranging from 2 : 1 to 9 : 1. No population had a dominance of tetrasporophytes, although two populations had 1 : 1 ratios. A meta-analysis of our data along with previously published accounts showed no significant changes in gametophyte dominance with respect to hypothesized gradients of wave exposure, salinity, or water depth. Significant changes occurred in ratios at five sites where replicate sampling occurred in different years. We conclude that C. crispus in Maritime Canada has a natural ratio of 3 : 1 or greater in stable conditions, and that lower ratios represent recovery from disturbance in which bare substratum is created that is subsequently colonized by carpospores from remaining gametophytic thalli.

• Ocean and Polar Research
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• v.23 no.4
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• pp.411-417
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• 2001

Due to international law the Antarctic is currently the best protected large ecosystem on earth, providing the opportunity for scientific research into processes of both regional and global importance. However, it is impossible to carry out research activities without minor disturbances to the environment. The Weddell Sea with its shelf inhabiting fauna can be considered to be representative for the entire Antarctic shelf with exceptions. It has generally escaped major anthropogenic impact but it is the only area in the high latitude Antarctic where long-term research fishing has been carried out. There are two main results combining aspects of nature conservation and benthos research. Firstly, the use of dredges has clearly decreased over the last two decades, whilst the use of non-invasive underwater photography and video has significantly increased. Secondly, during the same period icebergs destroyed an area of the seafloor and its fauna more than 2000-times greater than the area affected by research trawls. The increased use of imaging methods, Remotely Operated Vehicles (ROV) and other modem instruments, as well as statistically based and coordinated sampling strategies can contribute to both a better understanding of ecosystem function and to an ongoing reduction in anthropogenic impact.

• Korean Journal of Ecology and Environment
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• v.50 no.3
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• pp.325-336
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• 2017

The ecosystem in the Gwangju Stream has taken a wide range of disturbance such as the discharging water of sewage treatment plant, the lake water and the river water from different water system over the past decade. This study was figured out some significant influence factors by analyzing the relationship between biotic and abiotic factors in the urban stream. Abiotic components included 15 water quality variables which were measured in five sampling sites along the stream from October 2014 to July 2015, whereas the benthic macroinvertebrates found in those sites were used to estimate various biotic indices representing the ecological status of the community. The results of correlation analyses indicated that abiotic factors by human activities affected on the inhabitation of benthic macroinvertebrates more than biotic factors. The results of cluster analyses and ANOVA tests also showed that biotic and abiotic characteristics were clearly different in season. The main influence factors of cluster analysis by sites were $NH_3-N$, EPT(I) and DO. It was considered that more various statistical analyses would be necessary to find some different relationships and influence factors between biotic and abiotic variables in the urban stream.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.1124-1131
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• 2008

Thanks to a new in-situ seismic probe, using bender elements and penetration scheme, a simple linear relationship between undrained shear strength(Cu) and shear wave velocity(Vs) was obtained. This priceless relationship is worthy to be illuminated further in ideal laboratory environment. To avoid sampling disturbance effect, special consolidation cylinders were used to make normally consolidated specimens from kaolinite suspension. The undrained shear strengths of the specimens were measured using unconsolidated undrained triaxial compression tests. Also shear wave velocity measurements were performedprior to shearing the same specimens, using the bender elements installed in the base pedestal and the top cap of the triaxial compression cell. The Cu-Vs relationship is fairly linear and supports the linear trend of clayey silt obtained using field testing. Also the classic density-shear modulus relationship for soft clay proposed by Hardin and Black(1969) was once more verified hereby.