• Journal of Life Science
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• v.21 no.10
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• pp.1487-1493
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• 2011

E. tarda, a fish pathogen, can survive in seawater under relatively high salt conditions as well as in fish under physiological salt conditions. Bacterial growth under different salt concentrations may influence the expression of genes involved in bacterial structure and physiology. The growth rate of E. tarda culture in high salt (3.5% NaCl) was similar to that in low salt (1.0% NaCl, physiological salt concentration). Interestingly, the strain moved much faster in low salt conditions than in high salt conditions. Electron microscopic observation demonstrated that the bacterial cells grown in high salt had less or no flagellation. Obvious flagellation was observed in the parental strain E. tarda CK41 grown in low-salt condition. Two putative genes coding flagellin were identified in the E. tarda genome sequences. The amino acid sequence comparison of each gene revealed 93% identities. A flagellin gene was PCR amplified and cloned into a cloning vector. Using an E. coli protein expression system, a part of flagellin protein was overexpressed. Using the purified protein, an anti-flagellin antibody was raised in the rabbit. Immunoblot analyses with flagellin specific antibody demonstrated that E. tarda CK41 expressed falgellin in low salt conditions, which is consistent with the results seen in motility assay and microscopic observation. This is the first report of salt regulated flagella expression in E. tarda.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.237-237
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• 2017

Rice is highly sensitive to salt stress especially in its early growth stage, which thus is one of the major constraints in rice production. In rice plants, salt sensitivity is associated with the accumulation of $Na^+$ in the shoots, especially in the photosynthetic tissues. High salt concentrations in soil cause high $Na^+$ and $Cl^-$ transport to the shoot and preferential accumulation of those ions in older leaves, which decreases $K^+$ in the shoot, photosynthetic activity and grain yield. Salt exclusion capacity at the leaf sheath is therefore considered to be one of the main mechanisms of salt tolerance. In addition, it is suspected that the lamina joint might be involved in the salt transport from leaf sheath to leaf blade. This research aims to determine if leaf sheaths of rice exclude a large amount of $Na^+$ only or other ions such as $K^+$, $Ca^{2+}$, $Mg^{2+}$, and $Cl^-$ as well, to identify tissues in the leaf sheath, which accumulate $Na^+$, and to examine if the lamina joint is involved in the salt exclusion by the leaf sheath. The rice seedlings of salt tolerant genotype FL478 and salt sensitive genotype IR29 were independently treated with NaCl, KCl, $MgCl_2$ and $CaCl_2$, and Taichung 65 and its near-isogenic liguleless line (T65lg) were treated with NaCl. Then, the content of $Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, and $Cl^-$ ions and their specific location were determined using Atomic Absorption Spectrometer, Ion Chromatograph, and Energy Dispersive X-ray Spectroscopy. Results showed that leaf sheaths of FL478 and IR29 accumulated a large amount of $Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, and $Cl^-$ ons, and thus excluded them from leaf blades when treated with high concentration of each salt. When treated with NaCl, the highest $Na^+$ concentration was found in the basal part of leaf sheaths of both cultivars. Moreover, energy-dispersive X-ray spectroscopy revealed that the central parenchyma cells of the leaf sheath were the site where most Na, Cl, and K were retained under salinity in the salt tolerant genotype FL478. Also, the concentration of $Na^+$, $K^+$ and $Cl^-$ ions in leaf sheaths and leaf blades was comparable between T65 and T65lg, indicating that the lamina joint may not be involved in the exclusion of $Na^+$, $Cl^-$ and $K^+$ by the leaf sheath from the leaf blade under salinity. Therefore, we conclude that the central parenchyma cells of basal part of leaf sheath are the site that plays a physiological role to exclude $Na^+$ in the shoots of rice without the involvement of the lamina joint.

• Food Industry And Nutrition
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• v.9 no.1
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• pp.36-45
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• 2004

Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly inhibited the ear swelling response and histamine release induced by compound 48/80 in mice and rat peritoneal mast cells. Purple bamboo salt (0.01 ∼ lg/kg) also dose-dependently inhibited the passive cutaneous anaphylaxis by oral administration. Purple bamboo salt (1 mg/mL) in hibited phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$ and IL-6 secretion, by 67.04${\pm}$0.08%, 68.01${\pm}$1.85%, 69.48${\pm}$0.54%, respectively. In addition, purple bamboo salt inhibited the expression of TNF-${\alpha}$ mRNA in HMC-1 cells. Finally, we investigated the effect of purple bamboo salt in the forced swimming test (FST) and the change of purple bamboo salt-mediated cytokine production from MOLT-4 cells. At the 7th, immobility time was significantly decreased in the purple bamboo salt-administration group (35.4 ${\pm}$5.9 s for 1 g/kg) in comparison with the control group (93.2 ${\pm}$ 15.45). After FST, the content of glucose in the blood serum was increased and the levels of blood urea nitrogen, lactic dehydrogenase was decreased in purple bamboo salt-administration group. However, it had no effect on the elevation of CK and TP level. Purple bamboo salt (1 mg/mL) significantly increased the interferon (IFN)-${\gamma}$ and IL-2 level compared with media control (about 3.7-fold for IFN-${\gamma}$, about 3.5-fold for IL-2, p〈0.05) but did not affect the IL-4.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.6
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• pp.1054-1062
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• 2022

The population of Korea's solar salt-producing regions is rapidly aging, resulting in a decrease in the number of productive workers. In solar salt production, salt collection is the most labor-intensive operation because existing salt collection vehicles require human operators. Therefore, we intend to develop an unmanned solar salt collection vehicle to reduce manpower requirements. The unmanned solar salt collection vehicle is designed to identify the salt collection status and location in the salt plate via color detection, the color detection performance is a crucial consideration. Therefore, an image processing algorithm was developed to improve color detection performance. The algorithm generates an around-view image by using resizing, rotation, and perspective transformation of the input image, set the RoI to transform only the corresponding area to the HSV color model, and detects the color area through an AND operation. The detected color area was expanded and noise removed using morphological operations, and the area of the detection region was calculated using contour and image moment. The calculated area is compared with the set area to determine the location case of the collection vehicle within the salt plate. The performance was evaluated by comparing the calculated area of the final detected color to which the algorithm was applied and the area of the detected color in each step of the algorithm. It was confirmed that the color detection performance is improved by at least 25-99% for salt detection, at least 44-68% for red color, and an average of 7% for blue and an average of 15% for green. The proposed approach is well-suited to the operation of unmanned solar salt collection vehicles.

• Journal of the Korean Society of Food Culture
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• v.16 no.4
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• pp.388-397
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• 2001

Wild edible plants are consumed as raw and/or processed. Analytical data for raw biologically functional compounds were relatively well established. The changes on functional compounds during processing are, however, not well studied. This study was carried out to investigate the change of the quality of wild edible plants, Pimpinella brachycarpa(Chamnamul) at various conditions. Samples were blanched at the salt concentration of 0%, 1% or 2% for 1, 2, and 3 minutes each. The biologically active compounds, vitamin C, ${\beta}-carotene$, chlorophyll, flavonoids, polyphenols and minerals were analyzed. The concentration of vitamin C in Chamnamul decreased as blanching time increased. Beta-carotene in Chamnamul showed high concentration when Chamnamul was treated under longer blanching time. Higher salt concentration of blanching water, however, resulted in the increased concentration of ${\beta}-carotene$ in the blanched Chamnamul. Higher salt concentration of blanching water also reduced the loss of total flavonoids and total polyphenols from the blanched Chamnamul. The change of colors in the blanching water seemed to be corresponding to those of total flavonoids and total polyphenols concentrations in the blanching water.

• Journal of Plant Biology
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• v.26 no.3
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• pp.161-171
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• 1983

To test whether the Grime's model on relationship between maximum standing crop plus litter (350~750g/$m^2$) and species density (10~30/0.25$m^2$) fit well or not, a total of 52 samples, with 4 replicate plots (0.5m$\times$0.5m each) per sample, was collected from various forests, grass lands and coastal salt marshes in midwestern part of central Korean peninsula from September to October in 1982. The result agrees well with the model for grass lands salt marshes, that is, shape of curve for the maximum standing crop (minus litter) against species density indicates normal distribution. The number of species was 11 for the grassland and 7 for the salt marshes within the range of 300g to 700g per square meter for the maximum standing crop. In forest stands, however, as the maximum standing crop of herbs increased the species density decreased. The Grime's model does not seem to fit with the resutls on forest stands of this study. It is examined further the relationships among the maximum standing crop, species density and eleven soil properties, and the possible cause of this discrepancy was disscused.

• Geosciences Journal
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• v.22 no.6
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• pp.1041-1052
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• 2018

A single-well push-drift-pull tracer test using two different tracers ($SF_6$ and salt) was performed at the Environmental Impact Test (EIT) site to determine suitable locations for monitoring wells and arrange them prior to artificial $CO_2$ injection and leak tests. Local-scale estimates of hydraulic properties (linear groundwater velocity and effective porosity) were obtained at the study site by the tracer test with two tracers. The mass recovery percentage of the volatile tracer ($SF_6$) was lower than that of the non-volatile tracer (salt) and increased drift time may make degassing of $SF_6$ intensified. The $CO_2$ leakage monitoring results for both unsaturated and saturated zones suggest that the $CO_2$ monitoring points should be located near points at which a high concentration gradient is expected. Based on the estimated hydraulic properties and tracer mass recovery rates, an optimal $CO_2$ monitoring network including boreholes for monitoring the unsaturated zone was constructed at the study site.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.27 no.5
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• pp.353-357
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• 2015

A new method is proposed for water resources using aquifers in coastal areas. These aquifers are generally filled with saline water due to seawater intrusion and consequently being left unutilized. Surface water can be injected into these aquifers and recovered for water quality enhancement and stored water. Injection and pumping wells are used. For this technique to be successful protection of pumping well from seawater intrusion is an essential issue. Salt water pumping can be used to prevent saline water upconing. Numerical analysis demonstrated that a properly designed and executed salt water pumping well can protect a freshwater pumping well from salt water intrusion.

• Korean journal of food and cookery science
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• v.26 no.5
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• pp.665-675
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• 2010

This study applied high-pressure extraction cooking, which has been proven superior in processibility and stock extraction, for the preparation of stock, which is widely used as a basic material of dishes, and examined the physicochemical and sensory properties of the extracted stock by adding salt. We observed changes in the chromaticity of brown stock according to salt content, and pH decreased with increased the salt content, suggesting that stock was acidified. Regarding the mineral content, Na was the highest, followed by K and P. Regarding total free amino acid content, the content of essential amino acids, enhancing free amino acids, and other amino acids also showed the highest level in S2, which contented 0.3% salt. In the sensory test, saltiness grew stronger with an increase in salt content, and overall palatability was highest in S2. Regarding ranking, preference was highest for S2. Utilize to data that study finding of above is actual in development for mass production of brown stock product licensed cooks' traditional mesh dipper technology quality that make mass production direction that change and normalizes product that can keep excellent taste and improve conservative property develop. Culture formation and our country foodservice industry development that reduce damage of informed people ashes through development of brown stock product that is changed in large quantity, and improve quality of food more positively contributing to reappear diversification of menu, systematization and taste of specialty store as well as can contribute in rationalization of informed people ashes purchase, personnel expense (labor power) curtailment, waste decrease etc. but is developed still more being utilized.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1533-1541
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• 2016

Samples of doenjang (a fermented soybean paste) were prepared with different types of salts; purified salt (PS), 3-year-aged solar salt (SS3), 1-year-aged solar salt (SS1), and bamboo salt (BS, 3^rd processing product). For starter doenjang samples, selected starters comprising two bacilli, one yeast, and one fungus were inoculated, whereas for non-starter doenjang samples, microorganisms present in rice straw were inoculated after enrichment. The doenjang samples were fermented for 13 weeks at 25℃. During the fermentation period, SS and BS doenjang samples showed higher bacilli counts as well as much lower yeast counts than PS doenjang. At 13 weeks, yeast counts of starter doenjang samples were 7.75, 5.69, 6.08, and 4.74 log CFU/g for PS, SS3, SS1, and BS doenjang, respectively. For non-starter doenjang samples, counts were 7.17, 5.05, 5.92, and 4.54 log CFU/g for PS, SS3, SS1, and BS doenjang, respectively. SS and BS promoted growth of bacilli but inhibited growth of yeasts compared with PS. Debaryomyces hansenii was the dominant yeast in PS doenjang, whereas Candida guilliermondii and Pichia sorbitophila were dominant in SS and BS doenjang. In the sensory evaluation, SS and BS doenjang scored better than PS doenjang. In conclusion, SS and BS seem better than PS for production of high-quality doenjang.