• Journal of Life Science
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• v.21 no.12
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• pp.1789-1794
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• 2011

Dendritic cells (DCs) are professional antigen-presenting cells playing key roles in immune sentinels as initiators of T-cell responses against microbial pathogens and tumors. Sarijang, a folk sauce containing extracts of Rhynchosia nulubilis, Ulmus davidiana roots, Allium sativum, and Rhus Verniaiflura bark, has been used as a nonspecific immunostimulant for cancer patients. However, little is known about its immunomodulating effects or their mechanisms. In this study, we investigated whether sarijang induces phenotypic and functional maturation of DCs. For this study, murine bone marrow-derived myeloid DCs were cultured in the presence of interleukin-4 (IL-4) and granulocyte-macrophage colony stimulating factor (GM-CSF), and the generated immature DCs were stimulated with sarijang or lipopolysaccharide (LPS). Our data indicated that sarijang significantly enhanced the expression of co-stimulatory molecules (CD80 and CD86) as well as major histocompatibility complex (MHC) II, as did LPS. The results provide new insight into the immunopharmacology of sarijang and suggest a novel approach to the manipulation of DC for therapeutic application.

• Korean Journal of Microbiology
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• v.21 no.4
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• pp.221-228
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• 1983

An improved procedure for the rapid purification of glucose-6-phosphate dehydrogenase from extracts of Saccharomyces cerevisiae was developed by using affinity chromatography. Among six affinty media tested, $NADP^+ -agarose$ and Affi-gel Blue were more effective than others (i.e., Affi-gel Red, AMP-agarose, ATP-agarose, and $NAD^+ -agarose$). Conditions to desorb the enzyme bound to the affinity media were examined to increase the purity as well as yield. The best result was obtained when the column was developed with a linear gradient of KCl (0-1.0M). In case of Affi-gel Blue, introduction of $NAD^+$ (15mM) washing step prior to the salt gradient was most effective to remove $NAD^+ -binding$ proteins. For a large scale preparation of G-6-P dehydrogenase higher recovery was obtained by Affi-gel Blue than $NADP^+ -agarose$, however, the purity of the enzyme was decreased by 10 times if the former was used as the affinity medium. The capacity of Affi-gel Blue for G-6-P dehydrogenase was found to be 5 times higher than that of $NADP^+ -agarose$. Furthermore Affi-gel Blue could be reused repeatedly and its preparation is relatively easier and less expensive than $NADP^+ -agarose$.

• Research in Community and Public Health Nursing
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• v.14 no.3
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• pp.507-519
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• 2003

Purpose: The purpose of this study was to identify the prevalence, health behaviors, and control of hypertension in rural areas in Korea. Method: A total of 927 subjects above age 20 were selected from the areas which fell under the jurisdiction of the 24 Community Health Center located in Chungcheongbuk-do. The employees in the Community Health Center visited and interviewed patients. Data were collected using a questionnaire from July to October 2002. Result: The result showed that women had higher hypertension prevalence rates than men and the increment of its rate leveled up according to age. The hypertension prevalence rate was significantly high when the monthly income was less than 1 million won, their type of the Medical Insurance was the Guardian, they were only able to read Korean characters, and they were bereaved of spouses. In the Health behavior related to hypertension, the hypertension group showed a significantly lower level than the non- hypertension group in terms of drinking rate, smoking rate, intake of salt and intake of meat. There was no significant difference in the exercise rate and coffee intake rate between these groups. In the degree of the obesity the hypertension group was significantly higher than the non-hypertension group. The factors related to hypertension were that the duration for the incidence of hypertension was 12 to 60 months and took up 41.2%. The places where the medical check-ups occurred were the Community Health Center at 46.6%, the medical institution was mostly hospitals recording 46.5%. There were 66.1% of the targets who knew well about their blood pressure and there were 64.7% people who received education about it. As for the education place, the rate of Community Health Center was mostly high and it stood at 77.0%. In the aspect of the management of hypertension, the targets who took medicine on a regular basis were up to 76.1% and the targets who measured blood pressure once a month happened to be about 46.1 %. The targets who always recorded their blood pressure were 3.8%, chest X-ray as a related examination of hypertension reached 32.6%, electrocardiogram examination was 36.2%, cholesterol and serum lipid examination took up 33.6%, and the eye ground examination took 7.3%, which showed the lowest level of all.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.

• Korean Journal of Construction Engineering and Management
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• v.9 no.4
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• pp.193-205
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• 2008

Quality is an important keyword representing the corporate competitiveness and image in today' s construction industry. Especially in concrete construction, any problems or defects in fresh concrete can significantly degrade the entire quality and performance of the facility built. Thus, adequate quality inspection and testing must be exercised over the fresh concrete, if concrete with the required strength, durability and appearance is to be obtained. The testing of concrete delivered to the construction job site involves testing of fresh concrete and performing strength tests on hardened concrete. The principal tests conducted on fresh concrete include the slump test and tests for air and salt content. The temperature of fresh concrete should be checked out hot or cold weather concreting. The 7-day and 28-day strength of hardened concrete are also determined by compression tests on usually cylinder samples. However, it is very complex and time-consuming process requiring a lot of efforts to document those on-site concrete testing results and to accumulate their historical data. The primary objective of this study is to suggest a unique PDA and web-based system which enables an on-site quality manager to effectively conduct the concrete inspection and testing, automatically document and accumulate the collected historical data, and promptly obtain the approval from supervisors. Finally, it is anticipated that the effective use of the proposed PDA and web-based system would be able to improve reliability of the concrete quality inspection and testing data as well as significantly reduce the approval process.

• Journal of Navigation and Port Research
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• v.35 no.5
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• pp.393-400
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• 2011

The residential complexes in waterfront provide the residents with pleasantness and comfort with their scenety and view in the surrounding watersides. However, it is currently true that there has little been established any designing plan utilizing the environmental characteristics of the waterfront. In this study, a survey was conducted on the residents of a Japanese waterfront. in Nishimiyahama and Lake Biwa, and conducted a comparative and analytic investigation regarding water-friendly activities, residents' consciousness of the housing environment, and reasons for the choice of residenve. From the results, we were able to obtain some suggestive points resulting from the differences of the aforementioned. We also fully comprehended that there are some issues and needs for solution for the environmental characteristics in the waterfront as well as for the damage incurred by salt breeze and insects. In addition, we realized that scenery and view toward watersides are becoming the important factors in the residents' consciousness regarding the housing environment. In case of planning housing complexes located in the waterfront, we keenly understood that "the size of a room", "ventilation", "view and daily lighting", and "securing privacy" are having effects on the residents' consciousness of their housing.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.5
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• pp.58-66
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• 2002

We present a planting plan of the buffer-forest belts created at the boundary area of the waste landfill site which is located in the coastal area of Kyubg-Gi province. In order to form a proper section of ground soil excavated from the sea and a forest which shows a distinction of the vegetation stratification, the planting plan with trees, sub-trees, shrubs, and seedlings (produced at a sprout cultivation place) is devised with an adjustment of planting density. 1. The preparation of mounding is required for planting at a waste landfill site. We first estimate an economical and efficient banking height together with the quantity of soil, and prepare a planting ground with excavated ground soil for the consideration of soil recycling. On the planting ground a banking with a height of 1.5-2m is produced by self-supported soil, playing a role in a salt blocking and an irritation layer of planting. Finally, an additional banking with a height of 2m is produced by qualified vegetation soil, forming a vegetation section with a total height of 6m. 2. Since the planning site is located in the border, the planting area is composed of two regions : one is an inclined face (slope 1 : 3) toward the inside of the landfill site and the other is an inclined face (slope 1 : 4) toward the inland. The buffer planting in the former (latter) region consists of wind break forest (mixed-landscape forest) within a width of less than 35m. 3. Based on the data obtained from the literatures and the investigation of local plants, we choose the 21 plant species (such as Pinus thunbergii, Pinus densiflora, Sorbus alnifolia, Albizzia julibrissin and etc.) and the additinal 7 species which are grown at a sprout cultivation palce of the SUDOKWON landfill site (Rosa rugosa, Quercus acutissima, Prunus armeniaca var. ansu., and etc.). Sub-trees with a height of above 2.5m and seedlings are planted with an interval of $1.5{\times}1.5m$ ($0.45roots/m^2$) and $0.5{\times}0.5m$ ($4roots/m^2$), respectively. Here, both trees exhibit communities planting with more than three rows. Shrubs are planted with $9-16roots/m^2$, depending on their size. Since this case study provides a reference of the planting beds as well as a planting plan at the SUDOKWON landfill site, it is not sufficient for the present plan to be utilized for the formation of buffer-forest belts which are used for the analysis of environmental factor and the reduction of environmental pollutants in the sea waste landfill site. Thus, further studies with the ecological basis are demanded for the environment planting restoration in the sea waste landfill site.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.10
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• pp.687-693
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• 2013

Coastal sand dunes have been seriously damaged caused by the development thoughtless for the environment and coastal erosion and destruction due to artificial structures like coast roads and breakwater. Hereupon, in this study we made a library of rhizobacteria that have the plant growth-promoting ability for plant rhizosphere of indigenous plants inhabiting in a coastal sand dune as well as the strong tolerance to salt, and evaluated the plant growth-promoting ability of these strains. Furthermore, we evaluated the effect of rhizobacteria on the growth rate of saline tolerant plants in sandy soil; selected out the most useful micro-organism for the restoration of a damaged sand dune. The effect of inoculation of strains selected from the first experiment on the growth of Peucedanum japonicum and Arundo donaxes planted in a coastal sand dune was evaluated. As a result, Bacillus aerius MH1RS1 had plant growth promoting activities: indole acetic acid (IAA) production, siderophores and 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity, and also had a salinity tolerance. Also, in case of Peucedanum japonicum, the length of stems and weights of roots were enhanced by the inoculation of B. aerius MH1RS1. Fresh weights of stems and weights of roots in experimental group were, in particular, increased by 25% comparing with the control group. For an Arundo donax in experimental group, plant length increased by 18%, and weight of roots by 20% which is significant.

• Economic and Environmental Geology
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• v.24 no.3
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• pp.319-327
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• 1991

Cheju is the biggest island in Korean peninsula, consisted entirely of volcanic rocks and pyroclastic sediments. The topography is characterized by wide basalt plain in the low altitude but at the center of island, basalt volcano rises 1,950m above sea-level. Surface drainage is very poor, therefore water supply has been dependent on ground water and natural springs. There are about 1,650 production wells and most of them yield $1,000{\sim}2,000mm^3/day$. According to increase of ground water use, saline water is intruded in the low altitude of coastal area. Specially in the eastern coastal area, the topography is extensively flat and the level of ground water is very close to sea-level, at which overuse of ground water has brought saline intrusion up to maximum 6km far from the coast. Hydrochemical monitoring on this salt water intrusion is now undertaken on long term base.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.243-251
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• 2011

BACKGROUND: Gypsum($CaSO_4{\cdot}2H_2O$) is known as an ideal amendment to improve soil quality of the reclaimed coastal land. Since gypsum has very high concentration of electron acceptor like ${SO_4}^{2-}$, its application might be effective on reducing $CH_4$ emission during rice cultivation, but its effect has not been studied well. METHODS AND RESULTS: The effect of gypsum on $CH_4$ emission and rice growth characteristics was studied by pot test, which was packed by reclaimed paddy soils collected from Galsa, Hadong, Gyeongnam province. Chemical-grade gypsum was applied in two soils having EC 2.25 and 9.48 dS/m at rates of 0, 0.5, 1.0 and 2.0%(wt/wt). $CH_4$ emission was characterized a week interval by closed chamber method during rice cultivation. $CH_4$ emission rate was significantly decreased with increasing salt accumulation and gypsum application levels. With increasing gypsum application, dissolved ${SO_4}^{2-}$ concentration in the leachate water was significantly increased, which might have suppressed $CH_4$ production in soil. Total $CH_4$ flux was dramatically decreased with increasing gypsum application. In contrast, rice yield was increased with increasing gypsum application and then achieved maximum productivity at 1.0% gypsum application in two soils. CONCLUSION(s): Gypsum is a very good soil amendment to suppress $CH_4$ emission in reclaimed coastal paddy soils, and improve rice productivity and soil properties. The optimum application level of gypsum is assumed at ca. 1% to improve soil productivity with reducing effectively $CH_4$ emission during rice cultivation.