• Title/Summary/Keyword: salt analysis

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Residue analysis of spinetoram and spinosad on paprika leaf using the modified QuEChERS pre-treatment methods

  • Kim, Young-Shin;Yang, Jun-Young;Jin, Na-Young;Yu, Yong-Man;Youn, Young-Nam;Lim, Chi-Hwan
    • Korean Journal of Agricultural Science
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    • v.44 no.4
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    • pp.487-494
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    • 2017
  • Spinosad and spinetoram are widely used insecticides for the control of lepidopteran larvae, leaf miners, and thrips; however, they might also have low toxicity toward beneficial insects like bees. Because these pesticides are easily photolyzed by ultraviolet radiation, the QuEChERS method, with its simple pretreatment procedure, is often used for analyzing residues of spinosad and spinetoram. The present study performed a residue analysis using a modified QuEChERS method by pretreating with ammonium salt. The limit of detection (LOD) of the modified method was 0.05 mg/kg and the limit of quantification (LOQ) was 0.25 mg/kg. The coefficient of determination ($R^2$) for the calibration curve was 0.999. Also, we examined any change in the adhesion of spinosad and spinetoram on the plants depending on a spray volume. The adhesion was approximately 70% when the spray volume was increased from 60 L to 120 L per 10 a whereas the adhesion was approximately 37% when the spray volume was increased from 125 L to 250 L. This showed that the amount of adhesion decreased with the higher spray volume. The efficacy result of spinetoram was that over 90% of Frankliniella occidentalis was controlled with the application volume of 125 L per 10 a. Therefore, the result of this study indicates that control of insects is effective and sufficient with a spray volume of 125 L per 10 a in paprika cultivation facilities.

Size-segregated Sources of Aerosol Estimated by Factor Analysis-For the Measurement using Drum Impactor at Gosan, Jeju Island in May 2002 (인자분석을 통한 대기 입자상 물질의 입경별 발생원 추정-Drum impactor를 이용한 2002년 5월 제주도 고산지역 측정을 중심으로)

  • Han, J.S.;Moon, K.J.;Kong, B.J.;Ryu, S.Y.;Kim, Y.J.
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.5
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    • pp.685-695
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    • 2004
  • Size-segregated measurements of aerosol composition are used to estimate the transport of natural and anthropogenic aerosols at Gosan site during May 2002. The results of measurement show that not only soil dust but also anthropogenic aerosols, including sulfur and enriched trace metals such as Pb, Zn, Cu, are transported to Gosan. This study combines the size- and time-resolved aerosol composition measurements with factor analysis in order to identify some source materials. As a result, coarse particles (2.5${\mu}m$~12${\mu}m$) are influenced by soil, sea-salt, coal, coal combustion, and nonferrous sources. But fine particles have different sources. The fine particles, which the diameter is from 0.56${\mu}m$ to 2.5${\mu}m$, are more affected by road dust, oil combustion, industry. municipal incineration, and ferrous metal sources. The very fine particles, from 0.09${\mu}m$ to 0.56${\mu}m$, mainly supplied by biomass burning, oil combustion, nonferrous and ferrous metal sources.

Genetic mapping and sequence analysis of Phi class Glutathione S-transferases (BrGSTFs) candidates from Brassica rapa

  • Park, Tae-Ho;Jin, Mi-Na;Lee, Sang-Choon;Hong, Joon-Ki;Kim, Jung-Sun;Kim, Jin-A;Kwon, Soo-Jin;Zang, Yun-Xiang;Park, Young-Doo;Park, Beom-Seok
    • Journal of Plant Biotechnology
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    • v.35 no.4
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    • pp.265-274
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    • 2008
  • Glutathione S-transferases (GSTs) are multifunctional proteins encoded by a large gene family divided into Phi, Tau, Theta, Zeta, Lambda and DHAR classes on the basis of sequence identity. The Phi(F) and Tau(U) classes are plant-specific and ubiquitous. Their roles have been defined as herbicide detoxification and responses to biotic and abiotic stresses. Fifty-two members of the GST super-family were identified in the Arabidopsis thaliana genome, 13 members of which belong to the Phi class of GSTs (AtGSTFs). Based on the sequence similarities of AtGSTFs, 11 BAC clones were identified from Brassica rapa. Seven unique sequences of ORFs designated the Phi class candidates of GST derived from B. rapa (BrGSTFs) were detected from these 11 BAC clones by blast search and sequence alignment. Some of BrGSTFs were present in the same BAC clones indicating that BrGSTFs could also be clustered as usual in plant. They were mapped on B. rapa linkage group 2, 3, 9 and 10 and their nucleotide and amino acid sequences were highly similar to those of AtGSTFs. In addition, in silico analysis of BrGSTFs using Korea Brassica Genome Project 24K oligochip and microarray database for cold, salt and drought stresses revealed 15 unigenes to be highly similar to AtGSTFs and six of these were identical to one of BrGSTFs identified in the BAC clones indicating their expression. The sequences of BrGSTFs and unigenes identified in this study will facilitate further studies to apply GST genes to medical and agriculture purposes.

Cloning And Characterization of Pathogen-Inducible EREBP-Like Transcription Factor(CaNR19) From Hot Pepper (Capsicum annuum L.)

  • Yi, So-Young;Kim, Jee-Hyub;Yu, Seung-Hun;Park, Doil
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.77.2-78
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    • 2003
  • An EREBP/AP2-type transcription factor (CaPFl) was isolated by DDRT-PCR following inoculation of soybean pustule pathogen Xanthomonas axonopodis pv. glycines Bra which induces HR on pepper leaves. Genomic Southern blot analysis revealed that the CaPFl gene is present as a single copy within the hot pepper genome. The deduced amino acid sequence of CaPFl has two potential nuclear localization signals, a possible acidic activation domain, and an EREBP/AP2 motif that could bind to a conserved cis- element present in promoter region of many stress-induced genes. The mRNA level of CaPFl was induced by both biotic and abiotic stresses. We observed higher-level transcripts in resistance-induced pepper tissues than diseased tissues. Expression of CaPFl is also induced upon various abiotic stresses including ethephon, MeJA, cold stress, drought stress and salt stress treatments. To study the role of CPFI in plant, transgenic Arabidopsis and tobacco plants which express higher level of pepper CaPFl were generated. Global gene expression analysis of transgenic Arabidopsis by cDNA microarray indicated that expression of CaPFl in transgenic plants affect the expression of quite a few GCC box and DRE/CRT box-containing genes. Furthermore, the transgenic Arabidopsis and tobacco plant, expressing CaPFl showed tolerance against freezing temperature and enhanced resistance to Pseudomonas syrnigae pv. tabaci. Taken together, these results indicated that CaPFl is a novel EREBP/AP2 transcription factor in hot pepper plant and it may has a significant role(s) in regulation of biotic and abiotic stresses in plant.

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The Effects of the Nano-sized Adsorbing Material on the Electrochemical Properties of Sulfur Cathode for Lithium/Sulfur Secondary Battery (나노 흡착제가 Li/S 이차전지용 유황양극의 전기화학적 특성에 미치는 영향)

  • Song, Min-Sang;Han, Sang-Choel;Kim, Hyun-Seok;Ahn, Hyo-Jun;Lee, Jai-Young
    • Journal of Hydrogen and New Energy
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    • v.13 no.4
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    • pp.259-269
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    • 2002
  • A battery based on the lithium/elemental sulfur redox couple has the advantage of high theoretical specific capacity of 1,675 mAh/g-sulfur. However, Li/S battery has bad cyclic durability at room temperature due to sulfur active material loss resulting from lithium polysulfide dissolution. To improve the cycle life of Li/S battery, PEGDME (Poly(ethylene glycol) dimethyl ether) 500 containing 1M LiTFSI salt which has high viscosity was used as electrolyte to retard the polysulfide dissolution and nano-sized $Mg_{0.6}Ni_{0.4}O$ was added to sulfur cathode as additive to adsorb soluble polysulfide within sulfur cathode. From experimental results, the improvement of the capacity and cycle life of Li/S battery was observed( maximum discharge capacity : 1,185 mAh/g-sulfur, C50/C1 = 85 % ). Through the charge-discharge test, we knew that PEGDME 500 played a role of preventing incomplete charge-discharge $behavior^{1,2)$. And then, in sulfur dissolution analysis and rate capability test, we first confirmed that nano-sized $Mg_{0.6}Ni_{0.4}O$ had polysulfide adsorbing effect and catalytic effect of promoting the Li/S redox reaction. In addition, from BET surface area analysis, we also verified that it played the part of increasing the porosity of sulfur cathode.

Deterioration Mechanism Interpretation and Surface Contaminant Analysis of the Five-Storied Stone Pagoda in Tapriri, Uiseong (의성 탑리리 오층석탑의 표면오염물 분석 및 손상메커니즘 해석)

  • Lee, Mi Hye;Chun, Yu Gun;Lee, Myeong Seong
    • Economic and Environmental Geology
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    • v.46 no.5
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    • pp.445-453
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    • 2013
  • The Uiseong Tapriri Five-Storied Stone Pagoda (National Treasure No. 77) is typical stone cultural heritage in Unified Silla Dynasty, Korea. The pagoda has been occurred black, brown discoloration and microcrack, exfoliation, granularity decomposition with white discoloration because of continuous weathering. As the results of analysis for the contaminants, chemical weatherings are generated that black contaminant by manganese oxide, brown contaminant by iron oxide, white contaminants by gypsum and taranakite. And physical weatherings, such as microcrack, exfoliation, are occurred by salt(gypsum) crystallization. Therefore, these need to remove the contaminants according to the conservation treatment manual, and regular monitoring using P-XRF to preserve long-term the Five-Storied Stone Pagoda at Tapri-ri, Uiseong.

Quality Characteristics of Yanggeng added with Pesticide Free Wild Grape (Vitis amurensis) Juice (무농약 산머루 즙을 첨가한 양갱의 품질특성)

  • Kang, Chang-Soo
    • Korean Journal of Organic Agriculture
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    • v.23 no.2
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    • pp.301-309
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    • 2015
  • This study was performed to investigate the quality characteristics of Yanggeng added wild grape (Vitis amurensis) juice. The yanggeng was prepared with pesticide-free wild grape juice, agar, sugar and salt. The yanggeng was made with various levels (0, 50, 100, 150, 200 g of wild grape juice in yanggeng, respectively) based on the total weight of water. It was estimated on Hunter's color value, texture profile analysis, and sensory characteristics of the Yanggeng. As the content of wild grape juice increased, The lightness (L) and yellowness (b) decreased and redness (a) increased. In texture profile analysis, hardness was increased; however, springiness, cohesiveness, gumminess and chewiness decreased with increasing levels of wild grape juice. The results of the sensory evaluation indicated that the Yanggeng containing the 150 g level of wild grape juice showed the highest preference scores in terms of color, taste, texture, flavor and overall acceptance. These results show that the yanggeng containing 150 g of wild grape juice is the better.

A Study of Characteristics on the Dissimilar Metals (STS 316L - Carbon Steel: ASTM A516-70) Welds made with GTAW (스테인리스강 STS 316L과 탄소강 A516-70의 이종금속 GTA 용접부 특성에 대한 연구)

  • Kim, Se Cheol;Shin, Tae Woo;Moon, In Joon;Jang, Bok Su;Koh, Jin Hyun
    • Journal of Welding and Joining
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    • v.33 no.4
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    • pp.37-43
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    • 2015
  • Characteristics of dissimilar metal welds between STS 316L and carbon steel ASTM A516 Gr.70 made with GTAW have been evaluated in terms of microstructure, ferrite content, chemical analysis, hardness and corrosion resistance. Three heat inputs of 9.00, 11.25, 13.00kJ/cm were employed to make joints of dissimilar metals with ER309 wire. Based on microstructural examination, the amount of vermicular type of ${\delta}$-ferrite was increased with increasing heat input due to the increase of Creq/Nieq in the second layer of welds. Based on the EDX analysis of weld metals, Cr and Ni content in the 2nd layer increased while those content in the first layer of welds decreased with heat inputs. Cellular solidification mode in the 1st layer and dendritic solidification mode in the 2nd layer due to different cooling rates were prevailed, respectively. Heat affected zone which formed hard microstructure showed higher hardness than the weld metal. The salt spray test of dissimilar metals weld joints showed that the carbon steel surfaces only corroded. The weight loss rate due to corrosion increased up to 100hours but it decreased above 100 hours. There was little difference in the weight loss caused by corrosion regardless of heat inputs.

Estimation of Quantitative Source Contribution of Ambient PM-10 Using the PMF Model (PMF모델을 이용한 대기 중 PM-10 오염원의 정량적 기여도 추정)

  • 황인조;김동술
    • Journal of Korean Society for Atmospheric Environment
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    • v.19 no.6
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    • pp.719-731
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    • 2003
  • In order to maintain and manage ambient air quality, it is necessary to identify sources and to apportion its sources for ambient particulate matters. The receptor methods were one of the statistical methods to achieve reasonable air pollution strategies. Also, receptor methods, a field of chemometrics, is based on manifold applied statistics and is a statistical methodology that analyzes the physicochemical properties of gaseous and particulate pollutant on various atmospheric receptors, identifies the sources of air pollutants, and quantifies the apportionment of the sources to the receptors. The objective of this study was 1) after obtaining results from the PMF modeling, the existing sources of air at the study area were qualitatively identified and the contributions of each source were quantitatively estimated as well. 2) finally efficient air pollution management and control strategies of each source were suggested. The PMF model was intensively applied to estimate the quantitative contribution of air pollution sources based on the chemical information (128 samples and 25 chemical species). Through a case study of the PMF modeling for the PM-10 aerosols, the total of 11 factors were determined. The multiple linear regression analysis between the observed PM-10 mass concentration and the estimated G matrix had been performed following the FPEAK test. Finally the regression analysis provided quantitative source contributions (scaled G matrix) and source profiles (scaled F matrix). The results of the PMF modeling showed that the sources were apportioned by secondary aerosol related source 28.8 %, soil related source 16.8%, waste incineration source 11.5%, field burning source 11.0%, fossil fuel combustion source 10%, industry related source 8.3%, motor vehicle source 7.9%, oil/coal combustion source 4.4%, non-ferrous metal source 0.3%. and aged sea- salt source 0.2%, respectively.

Source Identification of Ambient PM-10 Using the PMF Model (PMF 모델을 이용한 대기 중 PM-10 오염원의 확인)

  • 황인조;김동술
    • Journal of Korean Society for Atmospheric Environment
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    • v.19 no.6
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    • pp.701-717
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    • 2003
  • The objective of this study was to extensively estimate the air quality trends of the study area by surveying con-centration trends in months or seasons, after analyzing the mass concentration of PM-10 samples and the inorganic lements, ion, and total carbon in PM-10. Also, the study introduced to apply the PMF (Positive Matrix Factoriza-tion) model that is useful when absence of the source profile. Thus the model was thought to be suitable in Korea that often has few information about pollution sources. After obtaining results from the PMF modeling, the existing sources at the study area were qualitatively identified The PM-10 particles collected on quartz fiber filters by a PM-10 high-vol air sampler for 3 years (Mar. 1999∼Dec.2001) in Kyung Hee University. The 25 chemical species (Al, Mn, Ti, V, Cr, Fe, Ni, Cu, Zn, As, Se, Cd, Ba, Ce, Pb, Si, N $a^{#}$, N $H_4$$^{+}$, $K^{+}$, $Mg^{2+}$, $Ca^{2+}$, C $l^{[-10]}$ , N $O_3$$^{[-10]}$ , S $O_4$$^{2-}$, TC) were analyzed by ICP-AES, IC, and EA after executing proper pre - treatments of each sample filter. The PMF model was intensively applied to estimate the quantitative contribution of air pollution sources based on the chemical information (128 samples and 25 chemical species). Through a case study of the PMF modeling for the PM-10 aerosols. the total of 11 factors were determined. The multiple linear regression analysis between the observed PM-10 mass concentration and the estimated G matrix had been performed following the FPEAK test. Finally the regression analysis provided source profiles (scaled F matrix). So, 11 sources were qualitatively identified, such as secondary aerosol related source, soil related source, waste incineration source, field burning source, fossil fuel combustion source, industry related source, motor vehicle source, oil/coal combustion source, non-ferrous metal source, and aged sea- salt source, respectively.ively.y.