• Journal of Dental Anesthesia and Pain Medicine
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• v.23 no.3
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• pp.153-162
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• 2023

Background: Recent animal studies have suggested the role of GABA type A (GABA-_A) receptors in salivation, showing that GABA-_A receptor agonists inhibit salivary secretion. This study aimed to evaluate the effects of propofol (a GABA-_A agonist) on salivary secretions from the submandibular, sublingual, and labial glands during intravenous sedation in healthy volunteers. Methods: Twenty healthy male volunteers participated in the study. They received a loading dose of propofol 6 mg/kg/h for 10 min, followed by 3 mg/kg/h for 15 min. Salivary flow rates in the submandibular, sublingual, and labial glands were measured before, during, and after propofol infusion, and amylase activity was measured in the saliva from the submandibular and sublingual glands. Results: We found that the salivary flow rates in the submandibular, sublingual, and labial glands significantly decreased during intravenous sedation with propofol (P < 0.01). Similarly, amylase activity in the saliva from the submandibular and sublingual glands was significantly decreased (P < 0.01). Conclusion: It can be concluded that intravenous sedation with propofol decreases salivary secretion in the submandibular, sublingual, and labial glands via the GABA-_A receptor. These results may be useful for dental treatment when desalivation is necessary.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.5
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• pp.2234-2243
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• 2013

The purpose of this study was to measure using a six-step solution for taste recognition threshold and using a Wet-Test for salivary flow amount of the adults who reside in Daegu area and are more than their 40's old to find out the relationship between DMFT index and OHIP-14. The study result revealed that the practice of oral health gave an effect to taste recognition threshold, dietary habit gave an effect to salivary flow amount, and sweet and sour taste of taste recognition threshold and salivary flow amount gave effects to the DMFT index. Therefore, it was found that the person with good practice of oral health and dietary habit had good taste, salivary flow amount and DMFT index. According to this result, it is necessary to increase the practice of oral health, increase the sensitivity to taste recognition threshold, improve dietary habit, increase salivary flow amount and decrease DMFT index in order to improve OHIP-14. Dietary habit education has been performed without the evaluation on the individual's level of taste recognition threshold. Therefore, it is necessary to develop the education program to measure and notify the taste recognition threshold in the oral health education so that individual can adjust it by himself.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.1
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• pp.67-74
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• 2005

The aim of this study was to analyze the relationship between salivary flow, salivary buffer capacity, level of mutans streptococci and dental caries experience in Korean dental college students. A total of 81 students of the school of dentistry, Chonbuk national university, mean age of 26.1 years, were subjected to oral examination to establish the DMFT and DMFS according to WHO guidelines. Saliva samples from the students were collected for quantitating stimulated and unstimulated salivary flow rate. Buffering capacity was estimated using a commercial colorimetric strip test, and number of mutans streptococci was determined from stimulated saliva using a strip mutans test. The means of DMFT and DMFS were 6.57 and 12.65, respectively. The stimulated salivary flow rate was correlated with DMFT(${\acute{o}}\;=\;-0.219$) and high levels of salivary mutans streptococci were significantly correlated with higher DMFT and DMFS scores(P<0.05). Level of mutans streptococci was significantly correlated with dental caries experience, in both DMFT and DMFS score, and buffering capacity was inversely correlated to DMFT score. However, unstimulated salivary flow rate was not correlated with caries experience.

• Journal of the Korea Convergence Society
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• v.9 no.4
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• pp.99-110
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• 2018

The data were collected from 171 physiologic malodor patients diagnosed in KUMC halitosis control clinic between 2008 and 2016. We selected 11 independent variables and 3 dependent variables, then planned to extract some convergent common factors affecting their physiologic malodor. We thought that those extracted convergent common factors could be utilized when preparing the contents of oral malodor preventive program. We used multiple regression analysis and path analysis method, for the analysis of influence of 11 independent factors to three salivary dependent factors(resting salivary flow rate, salivary buffering capacity, salivary precipitation rate). We have presented the physiologic malodor patients' chracteristics by descriptive statistical analysis, and also statistically analysed convergent common factors influencing directly or indirectly to their three dependent factors. We could reason that the sex, the character, the intake habit of breakfast, and the regular food intake habit could affect resting salivary flow rate, salivary buffering capacity and salivary precipitation rate.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.6
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• pp.525-530
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• 2014

Transient receptor potential vanilloid subtype 1 (TRPV1) was originally found in sensory neurons. Recently, it has been reported that TRPV1 is expressed in salivary gland epithelial cells (SGEC). However, the physiological role of TRPV1 in salivary secretion remains to be elucidated. We found that TRPV1 is expressed in mouse and human submandibular glands (SMG) and HSG cells, originated from human submandibular gland ducts at both mRNA and protein levels. However, capsaicin (CAP), TRPV1 agonist, had little effect on intracellular free calcium concentration ($[Ca^{2+}]_i$) in these cells, although carbachol consistently increased $[Ca^{2+}]_i$. Exposure of cells to high temperature (> $43^{\circ}C$) or acidic bath solution (pH5.4) did not increase $[Ca^{2+}]_i$, either. We further examined the role of TRPV1 in salivary secretion using TRPV1 knock-out mice. There was no significant difference in the pilocarpine (PILO)-induced salivary flow rate between wild-type and TRPV1 knock-out mice. Saliva flow rate also showed insignificant change in the mice treated with PILO plus CAP compared with that in mice treated with PILO alone. Taken together, our results suggest that although TRPV1 is expressed in SGEC, it appears not to play any direct roles in saliva secretion via transcellular pathway.

• Journal of Korean society of Dental Hygiene
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• v.15 no.4
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• pp.679-685
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• 2015

Objectives: The purpose of the study is to investigate the change of the salivary flow rate, xerostomia, and oral health-related quality of life in the elderly people after the application of oral massage. Methods: The subjects were 101 elderly people at two senior welfare centers in Mokpo from November, 2012 to January, 2013 by the application of oral massage. The final subjects were 56 elderly people who participated in the massage more than 15 times of 20. They were measured for saliva flow rate, xerostomia, and OHIP. On the first and the last day, a self-reported questionnaire was completed by the elderly people. The questionnaire consisted of general characteristics of the subjects, oral health related knowledge, symptoms and behavior of xerostomia, and OHIP. OHIP included functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability, and handicap, and was measured by Likert 5 point scale. Results: The salivary flow rate of the elderly people increased after the implementation of the program. There was a significant improvement in xerostomia, functional limitation, physical pain, psychological discomfort, and social efficacy (p<0.001). Conclusions : The oral massage program enhanced the oral function of the elderly people, and had an influence on the improvement of oral health-related quality of life.

• BMB Reports
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• v.40 no.1
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• pp.29-35
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• 2007

During the past decade, many salivary parameters have been used to characterize disease states. Ghrelin (GAH) is recently-discovered peptide hormone secreted mainly from the stomach but also produced in a number of other tissues including salivary glands. The aim of this work was to examine the relationship between active (aGAH) and inactive (dGAH) ghrelin in the saliva and other salivary parameters in type II diabetic patients and healthy controls. Salivary parameters were assessed in a single measurement of unstimulated whole saliva from 20 obese and 20 non-obese type II diabetes patients, and in 22 healthy controls. Total protein and alpha-amylase were determined by colorimetric methods, and glucose by the glucose-oxidase method. Saliva aGAH and dGAH levels were measured using a commercial radioimmunoassay (RIA) kit. Salivary concentrations of aGAH and dGAH ghrelin were more markedly decreased in obese diabetic subjects than in the two other groups. Glucose and alpha-amylase levels were higher in diabetic subjects than in controls. Furthermore, there were correlations between GAH levels and BMI, and between GAH and blood pressure. However, there was no marked variability in saliva flow rates among the groups. These results indicate that measurement of salivary GAH and its relationship to other salivary parameters might help to provide insight into the role of ghrelin in diabetes.

• The korean journal of orthodontics
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• v.6 no.1
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• pp.47-54
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• 1976

This study was conducted to investigate the effects of multibanded orthodontic appliances on dental caries activity. The subjects consisted of 50 males and females with multibanded orthodontic appliance and the same number of caries free group. The following conclusions were obtained, 1. There was significant difference between the experimental group and control group in the tests of Snyder colorimetric test, acid :buffering capacity and oral glucose clearance test (at $5\%$ level of significance). 2. There was no significant difference between the experimental group and control group in the tests of unstimulated salivary flow fate, stimulated salivary flow rate and salivary viscosity test (at $5\%$ level of significance). 3. Dental caries activity was higher in experimental group than in control group.

• Korean Journal of Head & Neck Oncology
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• v.11 no.2
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• pp.153-157
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• 1995

The effects of radiation therapy on salivary function of parotid gland were studied 20 male patients by radiation for tumors of head and neck. Saliva samples were collected before, during the radiotherapy and follow up. Parotid salivary function was quantitatively evaluated using a sialometry, sialochemistry, and scintigraphy. Salivary flow rates decreased in all individuals after irradiation. Glands than were partially irradiated were more likely to have some residual function than fully irradiated glands. Sodium and chloride contents were increased in parotid saliva of radiation patients where as potassium content was not changed definitively, pH was not changed during radiotherapy, but increased during follow up period. Scintigraphic stimulatory response rates showed similar result as flow rates. These results showed that exclusion of partial parotid gland from radiation is necessary to prevent severe xerostomia.

• International Journal of Oral Biology
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• v.44 no.2
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• pp.62-70
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• 2019

Xylitol is well-known to have an anti-caries effect by inhibiting the replication of cariogenic bacteria. In addition, xylitol enhances saliva secretion. However, the precise molecular mechanism of xylitol on saliva secretion is yet to be elucidated. Thus, in this study, we aimed to investigate the stimulatory effect of xylitol on saliva secretion and to further evaluate the involvement of xylitol in muscarinic type 3 receptor (M3R) signaling. For determining these effects, we measured the saliva flow rate following xylitol treatment in healthy individuals and patients with dry mouth. We further tested the effects of xylitol on M3R signaling in human salivary gland (HSG) cells using real-time quantitative reverse-transcriptase polymerase chain reaction, immunoblotting, and immunostaining. Xylitol candy significantly increased the salivary flow rate and intracellular calcium release in HSG cells via the M3R signaling pathway. In addition, the expressions of M3R and aquaporin 5 were induced by xylitol treatment. Lastly, we investigated the distribution of M3R and aquaporin 5 in HSG cells. Xylitol was found to activate M3R, thereby inducing increases in $Ca^{2+}$ concentration. Stimulation of the muscarinic receptor induced by xylitol activated the internalization of M3R and subsequent trafficking of aquaporin 5. Taken together, these findings suggest a molecular mechanism for secretory effects of xylitol on salivary epithelial cells.