• Title/Summary/Keyword: salinity stress

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Use of East Deep Sea Water for the Increase of Functional Components of Ginseng (Panax ginseng C.A. Meyer) and Tomato (Lycopersicon eculentum L.) (인삼과 토마토의 기능성 성분 증진을 위한 동해 해양심층수의 이용)

  • Woo Cheon-Seok;Kang Won-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.331-335
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    • 2006
  • This experiment was conducted to investigate the effect of deep sea water on fruit quality and yield of tomato. In the deep sea water treatments, fruit growth and weight were decreased as the concentration of deep sea water increased. Especially, the fresh weight of second truss was decreased significantly than first truss. Soluble solid content was increased significantly in higher concentration treatment especially at 30mM and 40mM treatment. That was increased more in the first than in the second truss fruits. Most of hexose in fruits were glucose and fructose. The reason of increased glucose and fructose contents was the decline of growth because of salinity stress by deep sea water treatment. however deep sea water treatment increased the lycopene content, especially in 20mM treatment. It is assumed that deep sea water treatment cause induction and promotion of ethylene. The higher concentration of deep sea water to the solution, the eater fruit quality improvement was noticed. However, proportional yield reduction accompanied concentration, 20mM deep sea water improved fruit quality without a significant yield reduction. The Re content was the highest among ginsenosides in all treatments. The contents total of ginsenosides in all treatments, except EC 8 treatment, was higher than those in the controlled treatment. The PT/PD value was 1.31 of the lowest in the EC 8 treatment and was 2.52 of the highest in the EC6 treatment. Rf contents in high increase was detected at all treated ginseng roots.

In Vitro Selection and Characterizations of Gamma Radiation-Induced Salt Tolerant Lines in Rice (방사선을 이용한 내염성 계통의 기내선발 및 특징)

  • Lee, In-Sok;Kim, Dong-Sub;Hyun, Do-Yoon;Lim, Yong-Pyo;Lee, Young-Il
    • Journal of Plant Biotechnology
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    • v.29 no.4
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    • pp.247-252
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    • 2002
  • The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.

Functional Screening of Plant Genes Suppressed Salt Sensitive Phenotype of Calcineurin Deficient Mutant through Yeast Complementation Analysis (애기장대의 염해 저항성 관련 유전자의 기능적 선별)

  • Moon, Seok-Jun;Park, Soo-Kwon;Hwang, Un-Ha;Lee, Jong-Hee;Han, Sang-Ik;Nam, Min-Hee;Park, Dong-Soo;Shin, Dongjin
    • Journal of Life Science
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    • v.23 no.1
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    • pp.1-7
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    • 2013
  • Understanding salt tolerance mechanisms is important for the increase of crop yields, and so, several screening approaches were developed to identify plant genes which are involved in salt tolerance of plants. Here, we transformed the Arabidopsis cDNA library into a salt-sensitive calcineurin (CaN)-deficient ($cnb{\Delta}$) yeast mutant and isolated the colonies which can suppress salt-sensitive phenotype of $cnb{\Delta}$ mutant. Through this functional complementation screen, a total of 34 colonies functionally suppressed the salt-sensitive phenotype of $cnb{\Delta}$ yeast cells, and sequencing analysis revealed that these are 9 genes, including CaS, AtSUMO1 and AtHB-12. Among these genes, the ectopic expression of CaS gene increased salt tolerance in yeast, and CaS transcript was up-regulated under high salinity conditions. CaS-antisense transgenic plants showed reduced root elongation under 100 mM NaCl treatment compared to the wild type plant, which survived under 150 mM NaCl treatment, whereas CaS-antisense transgenic plant leaves turned yellow under 150 mM NaCl treatment. These results indicate that the expression of CaS gene is important for stress tolerance in yeast and plants.

Effects of Nitrogen Application on the Patterns of Amino Acids, Nitrogen Contents and Growth Response of Four Legume Plants under Saline Conditions (염분 환경하에서 4종 콩과식물의 생장, 아미노산 및 질소함량에 미치는 질소원의 영향)

  • 배정진;추연식;김진아;노광수;송종석;송승달
    • The Korean Journal of Ecology
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    • v.26 no.3
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    • pp.135-142
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    • 2003
  • Four legume plants showed better growth by the external nitrogen supply rather than the symbiotic nitrogen fixation only under salt additions. In case of Glycine max and Phaseolus angularis, total nitrogen contents decreased by high salinity level but their amino acid levels significantly increased with the increase of salt treatments and indicated high soluble-/insoluble-N ratios. Cassia tora and Albizzia julibrissin contained less amino acids than G. max and P. angularis but total N (esp. insoluble N fraction) increased with higher salt levels. Asparagine occurred as a main amino acid especially in G. max and P. angularis and can be seen as potential N-storage form in these plants. It might be play an important role for the osmoregulation mechanism under the saline condition. Meanwhile, to investigate what kinds of nitrogen sources are effective for overcoming salt stress on soybean plants, various N forms and concentrations (NH₄NO₃-N, NO₃-N, NH₄NO₃-N; 2.5 and 5 mM) were additionally supplied to the salt gradient medium. Soybean plants treated with NH₄NO₃-N showed the best growth up to 40 mM NaCl and NO₃- fed plants indicated good growth even at 80 mM NaCl treatments. Contrary to NH₄NO₃- and NO₃- fed plants, NH₄/sup +/- fed plants showed remarkable growth reduction and died by 40 and 80 mM NaCl treatments after the first harvest (15th day). Consequently, these results suggest that salt excluding and resistant capacities of soybean plants under NaCl treatments are increased in order of NH₄ - N, control, NO₃- N and NH₄NO₃- N depending on N concentration except NH₄- N treatments.

Effects of Electro-conductivity on Growth of Beet and Turnip in the Reclaimed Land Soil (간척지 토양에서 양액의 전기전도도가 비트 및 순무의 생장에 미치는 영향)

  • Jo, Ji-Young;Sung, Ho-Young;Chun, Jin-Hyuk;Park, Jong-Seok;Park, Sang-Un;Park, Young-Jun;Kim, Sun-Ju
    • Korean Journal of Environmental Agriculture
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    • v.37 no.3
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    • pp.197-206
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    • 2018
  • BACKGROUND: The present study aimed to examine the crops capable of growing and adapting to the external environment and various stresses of reclaimed agriculture land for the development of high value-added agricultural utilization technology based on reclaimed land through standardization and empirical study of cultivation environment for cultivating crops. METHODS AND RESULTS: Two crops namely turnips and beets were selected for the salt tolerance test of soil environmental conditions on reclaimed land. Turnip and beet seedlings were planted on the soil collected at the 'Seokmun' reclaimed land. There are five treatments such as non-treatment, 1.0, 2.0 (control), 4.0 and $8.0dS{\cdot}m^{-1}$ of EC. The contents of betacyanin in beet roots was highest in control and decreased with increasing salt concentration. The GSL contents in the turnip roots waswere highest at EC 2.0 and decreased with increasing salt concentration, whereas those in turnip leaves waswere high both in the non-treated control and atthe EC 1.0-treatment. But, tThere was, however, no statistical differences among the treatments. CONCLUSION: The degree of salt tolerance of crops was examined, and the limit EC iswas expected to be $3.0{\sim}4.0dS{\cdot}m^{-1}$ as reported to date. If the soil improvement is performed and irrigation systems are used in the actual reclaimed land, the EC of supplied irrigation will be low, and desalination effecttreatment by the lower EC of the supplied irrigation on the soil will lead to more favorable soil condition of the rhizosphere and cultivation environment offor the crops than those in the port experiment. Therefore, monitoring the salinity, water content and ground water level will enable prediction of the rhizosphere environment, and setting up irrigation management and supplying irrigation will lead to crop cultivation results that are close to normal.

Isolation and Characterization of Indole-3-acetic acid- and 1-aminocylopropane-1-carboxylyic Acid Deaminase-producing Bacteria Related to Environmental Stress (환경스트레스와 관련된 indole-3-acetic acid 및 1-aminocylopropane-1-carboxylyic acid deaminase 활성을 갖는 박테리아의 분리와 특성 연구)

  • Kim, Hee Sook;Kim, Ji-Youn;Lee, Song Min;Park, Hye-Jung;Lee, Sang-Hyeon;Jang, Jeong Su;Lee, Mun Hyon
    • Microbiology and Biotechnology Letters
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    • v.47 no.3
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    • pp.390-400
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    • 2019
  • In this study, strains isolated from soil samples collected from Busan, Changwon, and Jeju Island were examined to verify their abilities of phosphate solubilization and nitrogen fixation, production of indole-3-acetic acid (IAA), siderophore, and 1-aminocylopropane-1-carboxylyic acid (ACC) deaminase in order to select strains that promote plant growth and play a role in biocontrol of pests or pathogens. According to the results of this study, most of the isolated strains were found to have ability of phosphate solubilization, nitrogen fixation, IAA production, siderophore production, and production of ACC deaminase. These isolated strains might help plant growth by directly improving absorption of nutrients essential for phosphate solubilization and nitrogen fixation. In addition, they can promote plant growth and control resistance to plant diseases through extracellular enzyme activity and antifungal activity. In addition, most of the selected strains were found to survive in various environmental conditions such as temperature, salinity, and pH. Therefore, Pseudomonas plecoglossicida ANG14, Pseudarthrobacter equi ANG28, Beijerinckia fluminensis ANG34, and Acinetobacter calcoaceticus ANG35 were finally selected through a comparative advantage analysis to suggest their potential as novel biological agents. Further studies are necessary in order to prove their efficacy as novel biological agents through formulation and optimization of effective microorganisms, their preservation period, and crop cultivation tests.

Applications of Thermal Imaging Camera to Detect the Physiological States Caused by Soil Fertilizer, Shading Growth, and Genetic Characteristic (열화상 카메라 활용을 위한 토양비료, 차광생육, 유전특성 차이 관련 작물생리 원격탐지)

  • Moon, Hyun-Dong;Cho, Yuna;Jo, Euni;Kim, Hyunki;Kim, Bo-kyeong;Jeong, Hoejeong;Kwon, Dongwon;Cho, Jaeil
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1101-1107
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    • 2022
  • The leaf temperature is principally regulated by the opening and closing of stomata that is sensitive to various kinds of plant physiological stress. Thus, the analysis of thermal imagery, one of remote sensing technique, will be useful to detect crop physiological condition on smart farm system and phenomics platform. However, there are few case studies using a thermal imaging camera on the agricultural application. In this study, three cases are presented: the effect of lime fertilizer on the rice, the different physiological properties of soybean under shading condition, and the screening of soybean breeds for salinity tolerance characteristic. The leaf temperature measured by thermal imaging camera on the three cases was used effectively to the physiological change and characteristics. However, the thermal imagery analysis requires considering the accuracy of measured temperature and the weather conditions that affects to the leaf temperature.

Biocontrol Activities of Peribacillus butanolivorans KJ40, Bacillus zanthoxyli HS1, B. siamensis H30-3 and Pseudomonas sp. BC42 on Anthracnose, Bacterial Fruit Blotch and Fusarium Wilt of Cucumber Plants (Peribacillus butanolivorans KJ40, Bacillus zanthoxyli HS1, B. siamensis H30-3와 Pseudomonas sp. BC42에 의한 오이 탄저병, 박과류 과실썩음병과 오이 덩굴쪼김병의 생물방제 효과검정)

  • Jiwon Kim;Mee Kyung Sang
    • Research in Plant Disease
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    • v.29 no.2
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    • pp.188-192
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    • 2023
  • Abiotic and biotic stresses have been a serious threat to crop growth and productivity in the agricultural system. In this study, four strains (HS1, H30-3, KJ40, and BC42), which have biological activities related to disease suppression or alleviation of salinity and drought stresses, were tested for broad-spectrum biocontrol activity against anthracnose caused by Colletotrichum orbiculare, a bacterial fruit blotch caused by Acidovorax citrulli, and Fusarium wilt caused by Fusarium oxysporum in cucumber plants. As a result of test, when the four strains were drenched into the soil, anthracnose in cucumber leaves significantly decrease; strain KJ40 suppressed disease incidence by A. citrulli; strain BC42 significantly reduced bacterial fruit blotch and Fusarium wilt compared to control. Therefore, strain KJ40 could be a biocontrol candidate for controlling anthracnose through induced systemic resistance and the disease caused by A. citrulli as well as alleviating drought stress; strain BC42 has broad-spectrum biocontrol activity against anthracnose, Fusarium wilt, and bacterial fruit blotch.

Evaluation of Salt Tolerance of Three Foliage Plant as affected by Salinity Concentration in Indoor Ornamental Hydroculture (실내 관상용 물재배에서 염분농도에 따른 3가지 관엽식물의 내염성 평가)

  • Jin Hee Ju;Sun Young Park;Yong Han Yoon
    • Journal of Environmental Science International
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    • v.33 no.4
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    • pp.257-268
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    • 2024
  • This study investigated the growth characteristics of Euonymus japonicus, Hedera helix, and Peperomia puteolata treated with different calcium chloride (CaCl2) concentrations to evaluate salt tolerance limits in hydroculture cultivation. Six concentrations of CaCl2 (0, 1, 2, 5, 10, and 15 g·L-1 referred to as Cont., C1, C2, C5, C10, and C15) were applied to solution - grown plant species. The survival rate, growth index, plant height, plant width, leaf width, leaf length, number of leaves, and relative chlorophyll contents were measured at monthly intervals. Euonymus japonicus, Hedera helix, and Peperomia puteolata survived up to C2, C5, and C10 at each CaCl2 concentration. The Euonymus japonicus was higher in the C1 treatment than in the Cont. for most growth characteristics. Hedera helix had the highest leaf width, leaf length, and number of leaves in the Cont., a significant difference was observed compared with the C1 treatment. The chlorophyll content did not differ significantly between the C5 and Cont. treatments. The leaf width and length of Peperomia puteolata were greater in the C2 and C1 treatments than in the Cont., whereas the number of leaves and chlorophyll content were the highest in C5. Dry weight analysis revealed that Euonymus japonicus, Hedera helix, and Peperomia puteolata were the lowest in the Cont. treatments. Euonymus japonicus was 74% in C15, and Hedera helix, and Peperomia puteolata were analyzed at approximately 37%- 50% and 9%-14%, respectively, regardless of the concentration in the CaCl2 treatment groups. In indoor hydroponic cultivation, the salt tolerance limit concentrations of Euonymus japonicus, Hedera helix, and Peperomia puteolata are 2, 5, and 10 g·L-1, respectively, indicating that hydroculture management techniques should be applied at higher concentrations.

Selection of Salt-Tolerant Silage Rice Through in vitro Screening and Saltol QTL Analysis (기내 선발과 Saltol QTL 분석을 통한 내염성 증진 사료용 벼 선발)

  • Cho, Chuloh;Kim, Kyung Hwa;Ahn, Eok-Keun;Park, Hyangmi;Choi, Man-Soo;Chun, Jaebuhm;Seo, Mi-Suk;Jin, Mina;Kim, Dool-Yi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.3
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    • pp.214-221
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    • 2020
  • Salinity is one of the major abiotic stressors that inhibits the growth, yield, and productivity of crop plants. Therefore, it is necessary to develop crops with increased salt tolerance for cultivation in saline soils such as is found in reclaimed land. The objective of this study was to develop a salt-tolerant silage rice line that grows on reclaimed land. In order to develop this salt-tolerant silage rice, we transferred Saltol, a major QTL associated with salt tolerance, from IR64-Saltol, a salt-tolerant indica variety, into Mogyang, a susceptible elite japonica variety. To determine the effect of salt stress, Mogyang and IR64-Saltol cultivars were grown on a medium containing various concentrations of NaCl in in vitro conditions. Shoot length was found to decrease with increasing salt concentrations, and root growth was almost arrested at NaCl concentrations over 50 mM in the Mogyang cultivar. Based on these preliminary results, we screened five salt-tolerant lines showing superior growth under salt stress conditions. Polymerase chain reaction and sequencing results showed that the introgression types of Saltol QTL were derived from the IR64-Saltol cultivar in almost all selected lines. Based on the observed growth and physiological characteristics, the new Saltol introgression lines showed higher salt tolerance compared to the Mogyang parental cultivar. The salt-tolerant lines identified in this study could be used as a genetic resource to improve rice salt tolerance.