• Journal of Biosystems Engineering
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• 제27권3호
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• pp.185-194
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• 2002

The objective of this study was to analyze a stability of sideways overturning of a forwarder developed by the Forestry Research Institute. The stability analysis was conducted using a multibody dynamic analysis program. VisualNastran Desktop. A solid model of the forwarder was constructed and its physical properties such as mass, mass center and mass moment of inertia were determined on 3D CAD modeler of the Solid Edge 8.0. 3D simulations of sideways overturning of the forwarder were also performed on the Visual Nastran using the solid model when it traveled across the slope and traversed over an up-hill side obstacle. Stability comparison between a bogie-wheeled and a 6-wheeled forwarders was also made and found that the bogie-wheeled forwarder was more stable than the 6-wheeled one on slopes. The safety speeds of the forwarder predicted by the simulation under various conditions can be used as a guideline for safe operations of forwarders in mountainous area. The technique of using a solid model for the sability analysis can also be applied successfully to other vehicles like agricultural tractors, loaders and construction equipments.

• Journal of Biosystems Engineering
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• 제28권2호
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• pp.117-126
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• 2003

This study was conducted to develop precision control system fur cold storage facility that could offer safe storage environment for green grocery. For that reason of neuro-fuzzy control system with learning ability algorithm and single chip neuro-fuzzy micro controller was developed for cold storage facility. Dynamic characteristics and hunting of neuro-fuzzy control system were far superior to on-off and fuzzy control system. Dynamic characteristics of temperature were faster than on-off control system by 1,555 seconds(123% faster) and fuzzy control system by 460 seconds(36.4% faster). When system was arrived at steady state. hunting was ${\pm}$0.5$^{\circ}C$ in on-off control system, ${\pm}$0.4$^{\circ}C$ in fuzzy control system, and ${\pm}$0.3$^{\circ}C$ in neuro-fuzzy control system. Hunting of humidity and wind velocity was also controlled precisely by 70 to 72.5% and 1m/s For storage experiment with onion, characteristics of neuro-fuzzy control system were tested. Dynamic characteristics of neuro-fuzzy control system made cold storage facility conducted precooling ability and minimized hunting.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.II
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• pp.480-488
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• 2000

Cabbage is the most important vegetables in korea. The cabbage production was based on arduous human labor. A comprehensive research for substituting the human work by machines has been performed at present. In general, cabbage is cultivated on hillside in korea. The harvested cabbage in a field and carrying it to a vehicle for transportation are very laborious work. Hand labor in cabbage transportation to the market damages the quality of cabbage and is also a cause to increase the cost of cabbage production. This study was to design and evaluate a prototype cabbage loader for deserving efficient and safe transportation of cabbage. The developed cabbage loader was a semi-tracked vehicle operated by a hydraulic system, allowing the safe transporting and the loading of cabbage in a steep field. The maximum loading capacity of the loader was 1.0 ton. By using safety devices attached to the loader, the static slopes were 34.0% and 37.4% for the left and the rear roll-over, respectively. The maximum field speed was about 6km/hr with two cabbage pallets of 750kg at a 25% inclined field. The field capacity was about 35 pallets/hr in case of picking up, carrying and unloading two cabbage pallets. The field efficiency of the loader was analyzed to be more than 8 times in comparison of the conventional human labor. The developed loader would be applied for loading and carrying the other vegetables due to the similarity of operations. The study suggested a standard approach to the design of field machines operated in a steep field.

• Journal of Biosystems Engineering
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• 제32권6호
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• pp.416-421
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• 2007

Physical damage to fruits and vegetables caused by shock degrades the value of product in the fresh market. In order to design a product/packaging system to protect the product, the G-factor to the product that causes shock damage needs to be determined. The shock fragility of organisms such as fruits with a concept correspondent to the G-factor of industrial products was calculated and we defined the allowable bio-shock fragility index as the value divided peak acceleration that was generated in safe drop height by standard acceleration of gravity. We did modeling for safe drop hight that would prevent fruits from damage by drop tests and tried to estimate the allowable bio-shock fragility index of pears and apples for optimum packaging design. The bio-shock fragility index of pears was in the range of $0.74{\sim}2.29\;G$, while apples had a slightly higher value than that of pears, of $0.51{\sim}2.98\;G$. This result shows accordance with the general fact that apples have a firmer structure and get less damage from the same impact. Based on this result, it is possible to create an optimum packaging design by providing a damage standard by impact.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.423-427
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• 2004

Seventy-six animals including cattle, sheep, horses, 6 species of zoo animals were employed for collection of fresh feces in order to detect verotoxigenic Esherichia coli (VTEC) by safe, quick and sensitive PCR-based molecular methods. Bacterial cell disruption with bead-beating followed by bacterial DNA purification with hydroxyapatide chromatography and gel filtration allowed DNA preparation from animal feces with high recovery and purity. A mountain goat was firstly shown by PCR and sequencing to shed verotoxin 2 gene (vt2) that was used to generate vt2 probe and second primer set for nested PCR to attempt more sensitive detection. Most sensitive nested PCR revealed that 45% of tested cattle and 47% of tested zoo animals were VTEC-positive, while least sensitive normal PCR detected VTEC from none of these animals except a mountain goat. Moderately sensitive detection by PCR in combination with hybridization suggested that the VTEC density varied between the VTEC-positive cattle.

• 한국농공학회논문집
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• 제57권5호
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• pp.13-17
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• 2015

There are many vinyl houses in rural areas. These vinyl houses have occasionally been collapsed due to heavy snow load in winter. If these vinyl houses are collapsed, many farmers get a lot of economical damages. So it is very important to built safe vinyl house that is able to withstand the applied heavy snow load. In this study, compressive buckling and flexural tests were performed to investigate the strength increase of circular mortar filled pipes. The results showed that buckling load and flexural moment of mortar filled pipes were increased 42 % ~ 82 %, 40 % ~ 44 % respectively more than only pipe without mortar. It is recommended that mortar filled pipes as main members of vinyl house have to be used to prevent collapsing due to the severe snow load.

• 생명과학회지
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• 제22권2호
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• pp.251-258
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• 2012

본 연구는 동물의 분변으로부터 분리한 유산균의 프로바이오틱 특성을 조사할 목적으로 시행되었다. 생리생화학적 특성과 16S 리보솜 DNA 분석 결과 BCNU 9041, 9042는 Lactobacillus brevis와 99%의 상동성을 나타내었다. 이들 균주를 대상으로 기초적인 안전성 실험을 시행한 결과, 이들 균주는 용혈현상이 나타나지 않으며 ${\beta}$-glucosidase, ${\beta}$-glucuronidase, tryptophanase 및 urease와 같은 유해한 생성물을 생성하지 않는 안전한 생물자 원임이 확인되었다. BCNU 9041 및 9042은 pH 2.5의 산성 조건 및 담즙에서(0.3, 0.6, 1%의 oxgall이 포함된 MRS broth) 높은 생존률을 나타내었다. 뿐만 아니라 식중독 원인 세균에 대하여 항균활성을 가지고 있으며, 특히 Bacillus cereus, Listeria monocytogenes 및 Shigella sonnei 에 대한 항균활성이 뛰어났다. 또한 BCNU 9041, BCNU 9042은 92-95%의 높은 소수성과 BSH (bile salt hydrolytic) 활성 및 cholesterol 흡수력이 우수함이 확인되었다. 이들 결과를 바탕으로 프로바이오틱로서의 우수한 기능성을 가진 BCNU 9041와 BCNU 9042의 기능성 식품 및 건강관련 제품으로의 활용이 기대된다.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1316-1323
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• 2017

Geosmin and 2-methylisoborneol (2-MIB), responsible for earthy or musty smell, are a major concern for safe drinking water supplies. This study investigated the effects of environmental factors on odorous compound production and cell growth in cyanobacterial strains. Anabaena sp. FACHB-1384, a 2-MIB producer, was sensitive to low temperature (<$20^{\circ}C$). However, geosmin producers, Anabaena sp. Chusori and Anabaena sp. NIER, were sensitive to high light intensity (>$100{\mu}mol/m^2/sec$), but not to low temperature. Geosmin concentrations increased under higher nitrate concentrations, being linearly proportional to cell density. A P-limited chemostat showed that P-stress decreased the geosmin productivity and extracellular geosmin amount per cell in Anabaena sp. NIER. However, only 2-MIB productivity was reduced in Planktothrix sp. FACHB-1374 under P-limitation. The extracellular 2-MIB amount per cell remained constant at all dilution rates. In conclusion, high light intensity and P-stress can contribute to the lower incidence of geosmin, whereas 2-MIB reduction could be attainable at a lower temperature.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1568-1577
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• 2015

The purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from Korean healthy infant feces and kimchi. Isolates A1, A2, S1, S2, and S3 were assigned to Lactobacillus sp. and isolates A3, A4, E1, E2, E3, and E4 were assigned to Leuconostoc sp. on the basis of their physiological properties and 16S ribosomal DNA sequence analysis. Most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmful enzymes such as β-glucosidase, β-glucuronidase and tryptophanase. The 11 isolates showed different resistance to acid and bile acids. In addition, they exhibited antibacterial activity against foodborne bacteria, especially Bacillus cereus, Listeria monocytogenes, and Escherichia coli. Furthermore, all strains showed significantly high levels of hydrophobicity. The antioxidant effects of culture filtrates of the 11 strains included 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2.2'-azino-bis (2-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity, and superoxide dismutase activity. The results revealed that most of the culture filtrates have effective scavenging activity for DPPH and ABTS radicals. All strains appeared to have effective superoxide dismutase activity. In conclusion, the isolated strains A1, A3, S1, and S3 have significant probiotic activities applicable to the development of functional foods and health-related products. These strains might also contribute to preventing and controlling several diseases associated with oxidative stress, when used as probiotics.

• 한국미생물·생명공학회지
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• 제50권3호
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• pp.361-374
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• 2022

For this study, we designed and produced mulberry low-fat ice cream supplemented with synbiotics (MLF-ISS). The sensory characteristics and physical, chemical, and microbiological qualities of MLF-ISS were then determined. Mulberry juice inoculated with or without probiotic (Lactobacillus plantarum TISTR 926 and Saccharomyces boulardii CNCM I-745) was also tested at 37℃ for 24 h to determine probiotic growth rate, pH, total anthocyanin content (TAC), total phenolic content (TPC), and antioxidant activity (AA). Only the TAC of mulberry juice inoculated with S. boulardii CNCM I-745 increased considerably (p < 0.05) among these parameters. MLF-ISS was produced with varied mulberry fruit concentrations (0, 10, 20, 30, or 40%) (w/w). The MLF-ISS prepared with 30% mulberry fruit (w/w) (30-MLF-ISS) had a higher score in appearance, color, and sweetness (p < 0.05) when sensory qualities were measured using the 9-point hedonic scale method. In the CIE lab system (L^*, a^*, b^*), the color values of 30-MLF-ISS were 27.80 ± 0.26, 12.99 ± 0.59, and 1.43 ± 0.05, respectively. The 30-MLF-ISS was also subjected to a proximate analysis. The melting rate of 30-MLF-ISS was 0.29 ± 0.03 g/min and the time it took for the first drop to fall was 37.00 ± 7.00 min. TAC, TPC, and AA of 30-MLF-ISS were observed to alter significantly (p < 0.05) during varied intervals of storage at - 18℃ (0, 30, and 60 days). The viability of probiotics in 30-MLF-ISS slightly decreased after storage at -18℃ for 8 weeks, but remained about 6 log CFU/g. During storage at -18℃ for 0 and 120 days, no pathogenic bacteria were detected in 30-MLF-ISS. These findings show that 30-MLF-ISS has nutritional and functional value, is free of foodborne pathogenic bacteria, is safe for consumers' health, and is suitable for application in the ice cream and related food industries.