AT(Aerial Triangulation) is the essential procedure for creating orthophoto and transforming coordinates on the photographs into the real world coordinates utilizing GCPs (Ground Control Point) which is obtained by field survey and the external orientation factors from GPS/INS as a reference coordinates. In this procedure, all of the GCPs can be collected from field survey using GPS and Total Station, or obtained from digital maps. Collecting GCPs by field survey is accurate than GCPs from digital maps; however, lots of manpower should be put into the collecting procedure, and time and cost as well. On the other hand, in the case of obtaining GCPs from digital maps, it is very difficult to secure the required accuracy because almost things at each stage in the collecting procedure should rely on the subjective judgement of the performer. In this study, the results from three methods have been compared for the accuracy assessment in order to know if the results of each case is within the allowance error: for the perceivable objects such as road boarder, speed bumps, constructions etc., 1) GCPs selection utilizing the unique LiDAR intensity value reflected from such objects, 2) using LiDAR DSM and 3) GCPs from field survey. And also, AT and error analysis have been carried out w ith GCPs obtained by each case.
Two hybridoma cell lines against Cwptosporinium possum oocysts nFRl-CN911 were produced. The isotype of these 2 monoclonal antibodies (mAbs) was IgG2b (lE7.2) and and IgM (C6). Enzyme immuno-transfer blotting analysis showed that 157.2 reacted specifically to 36 kDa protein and C6 reacted to 67 and 70 kDa proteins. C. pcnlum was bound specifically to the surface region of oocysts by these mobs. No cross-reactivity was observed with tachyzoites of ToxopLosma gonnii and oocysts of Eimeria zuernii,5. bouis and E. canadensis of bovine origin. The indirect immunofluorescence assay (IIF) using mAb C6 was successful with counterstain. With the IIF using mob C6, oocysts appeared as 3 to $5{\mu}m$ spherical objects fluorescing bright apple green against a reddish dark background. The IIF using mAb C6 was agreed in specificity and sensitivity with those of a commercial diagnostic kit. These results demonstrated that the produced mAbs were specific to C. parvum and that the mAb C6 could be used for diagnosis of cryptosporidiosis.
Leptin, the hormone product of the obese gene, is secreted predominately from white adipose tissue and regulates feed intake, energy metabolism and body composition. It has been considered a candidate gene for performance, carcass and meat quality traits in beef cattle. The objective of this study was to identify SNPs in the promoter region of the leptin gene and to evaluate the possible association of the SNP genotypes with carcass and meat quality traits in Korean cattle. We identified a total of 25 SNPs in the promoter region (1,208-3,049 bp upstream from the transcription start site) of the leptin gene, eleven (g.1508C>G, g.1540G>A, g.1545G>A, g.1551C>T, g.1746T>G, g.1798ins(G), g.1932del(T), g.1933del(T), g.1934del(T), g.1993C>T and g.2033C>T) of which have not been reported previously. Their sequences were deposited in GenBank database with accession number DQ202319. Genotyping of the SNPs located at positions g.2418C>G and g.2423G>A within the promoter region was performed by direct sequencing and PCR-SSCP method to investigate the effects of SNP genotypes on carcass and meat quality traits in Korean cattle. The SNP and SSCP genotypes from the two mutations of the leptin promoter were shown to be associated with the BF trait. The average BF value of animals with heterozygous SNP genotype was significantly greater than that of animals with the homozygous SNP genotypes for the g.2418C>G and g.2423G>A SNPs (p<0.05). Analysis of the combined genotype effect in both SNPs showed that animals with the AC SSCP genotype had higher BF value than animals with BB or AA SSCP genotypes (p<0.05). These results suggest that SNP of the leptin promoter region may be useful markers for selection of economic traits in Korean cattle.
Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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v.4
/
pp.395-411
/
2001
Die $W\"{o}rterb\"{u}cher$ spielen beim Erwerb einer Sprache eine wesentliche Rolle. Es gibt inzwischen mehrere Deutsch-Koreanische $W\"{o}rterb\"{u}cher$. Die Lage $f\"{u}r$ die Koreanisch-Deutschen $W\"{o}rterb\"{u}cher$ ist aber verbesserungs- $bed\"{u}rftig$. Stellvertretend $f\"{u}r$ das Koreanisch/Deutsche $W\"{o}rterbuch$ steht das 1981 von der Koreanischen Gesellschaft $f\"{u}r$ Germanistik nach $zehnj\"{a}hriger$ Arbeit fertiggestellte Essence Koreanisch-Deutsche $W\"{o}rterb\"{u}ch$ (Minjung seorim 1982). Dieses $W\"{o}rterb\"{u}ch$ weist aber einige Nachteilen auf. Es fehlen jegliche Angaben zum Korpus. Man $st\"{o}{\ss}t$ beispielsweise oft auf selten gebrauchte $W\"{o}rter$, die Grundbedeutungen sind nicht erkennbar, die Erklarungen sind ungenau, manchmal sogar $irref\"{u}hrend\;und\;schlie{\ss}lich$ mitunter stilistisch falsch. Diese negativen Punkte wirken sich $ung\"{u}nstig$ auf den gesamten Lemprozess bis hin zur Lemmotivation aus. Aus diesen Gegebenheiten $erw\"{a}chst$ die Notwendigkeit der Erstellung eines neuen $W\"{o}rterb\"{u}ch$s, das auf die Situation von Sprachlemem abgestimmt ist, die zur Benutzung einsprachiger $W\"{o}rterbticher$ noch nicht imstande sind. Zweck des $W\"{o}rterb\"{u}chs$ ist der Einsatz im Fremdsprachenunterricht, woraus sich ein zweisprachiges $W\"{o}rterb\"{u}ch$ ergibt. Als Benutzerkreis werden insbesondere $Sprachlemanf\"{a}nger$ des Koreanischen und Deutschen ins Auge gefasst. Ihnen soll ein Nachschlagewerk an die Hand gegeben werden, das den koreanischen und deutschen Wortschatz anhand einer Systematik und eines alphabetischen Indexes der jeweiligen Sprache $erschlie{\ss}t$. Ais Zahl der Lemmata wird $f\"{u}r$ jede Sprache ca. 10,000 festgelegt. Ein Grundwortschatz, der die 3000 $h\"{a}ufigsten\;W\"{o}rter$ umfasst wird extra markiert. $Dar\"{u}ber$ hinaus bietet das $W\"{o}rterb\"{u}ch$ den darauf aufbauende Wortschatz von weiteren 7000 $W\"{o}rtern$. Die Teamarbeit der beiden Gruppen von Muttersprachlern steht in allen Arbeitsschritten im Vordergrund. Der damit verbundene Zweck ist die Erzielung der maximalen Genauigkeit der $Lemmaerkl\"{a}rungen$. Da dieses $W\"{o}rterb\"{u}ch$ ein $W\"{o}rterb\"{u}ch$ nach Sachgruppen ist, die Erstellung einer Systematik von $gro{\ss}er$ Bedeutung. $F\"{u}r$ das vorliegende Projekt gait daher eine Systematik zu finden, die nicht nur leicht nachvollziehbar, also praktikabel ist, sondern insbesondere von Benutzern aus zwei Kulturkreisen akzeptiert werden kann. Als $grunds\"{a}tzliche$, allerdings nicht in allen Bereichen $gleicherma{\ss}en$ anwendbare Ordnungsprinzipien gelten: Vom Oberbegriff zum Unterbegriff, vom allgemein zum speziell, vom Einfach zum Komplexen, von $au{\ss}en$ nach innen, Darin spiegelt sich das Prinzip der 'notwendigen Bedingung' wider.
In this study, variable characteristics of drinking water and the influences on underground water reservoirs, rooftop water tanks, and service water pipes in the building were assessed. The influence of underground water reservoir material and water capacity on water quality also were assessed. The results are the following as; First of all, the drinking water passing through underground water reservoirs or service water pipes in the building, averagely metal component concentration more increased from percent of 41.3 to percent of 74.2 totally than other items of water quality. On the other hand, both residual chlorine and total solid highly decreased 65.6 percent and 35.3 percent, respectively. Therefore, it was thought that water quality could be getting worse for microorganism re-growth by residual chlorine reduction, and total solid also could be a cause for extraneous matters accumulated in water reservoir. Secondly, the variations on water quality of each stage for water supply system in the building were higher in water service pipes connected from rooftop water tanks to the tap than in underground water reservoirs. In addition to, among of twelve items on water quality, ten items on water quality except dissolved oxygen and residual chlorine increased. Therefore, it was thought that the influence of water service pipes connected from rooftop water tanks to the tap on water quality were higher than other stages of water supply system in the building. Thirdly, in case of materials of underground water reservoir, it was likely that the variation on water quality by stainless steel and concrete materials got some similar. In case of water capacity, the variations on water quality of underground water reservoirs over $1,000m^3$ higher than those under $1,000m^3$. That reasons was likely that the retention time(49.72 hours averagely) of underground water reservoirs over $1,000m^3$ was two times longer than it of those under $1,000m^3$(23.37 hours). Therefore, it was thought that the influence on water quality by materials were some similar, but in case of water capacity, the influence of underground water reservoirs were higher.
Journal of The Korean Society of Inherited Metabolic disease
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v.6
no.1
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pp.15-23
/
2006
Purpose: Glycogen storage disease type III (GSD-III), is a rare autosomal recessive disorder of glycogen metabolism. The affected enzyme is amylo-1,6-glucosidase, 4-alpha-glucanotransferase (AGL, glycogen debranching enzyme), which is responsible for the debranching of the glycogen molecule during catabolism. The disease has been demonstrated to show clinical and biochemical heterogeneity, reflecting the genotype-phenotype heterogeneity among different patients. In this study, we analyzed mutations of the AGL gene in three unrelated Korean GSD-III patients and discussed their clinical and laboratory implications. Methods: We studied three GSD-III patients and the clinical features were characterized. Sequence analysis of 35exons and part exon-intron boundaries of the AGLgene in patients were carried out by direct DNA sequencing method using genomic DNA isolated from patients' peripheral leukocytes. Results: The clinical features included hepatomegaly (in all patients), seizures (in patient 2), growth failure (in patients 1), hyperlipidemia (in patients 1 and 3), raised transaminases and creatinine kinase concentrations (in all patients) and mild EKG abnormalities (in patients 2). Liver transplantation was performed in patient 2due to progressive hepatic fibrosis. Administration of raw-corn-starch could maintain normoglycemia and improve the condition. DNA sequence analysis revealed mutations in 5 out of 6 alleles. Patient 1 was a compound heterozygote of c.1282 G>A (p.R428K) and c.1306delA (p.S603PfsX6), patient 2 with c.1510_1511insT (p.Y504LfsX10), and patient 3 with c.3416 T>C (p.L1139P) and c.l735+1 G>T (Y538_R578delfsX4) mutations. Except R428K mutation, 4 other mutations identified in3 patients were novel. Conclusion: GSD-III patients have variable phenotypic characteristics resembling GSD-Ia. The molecular defects in the AGL gene of Korean GSD-III patients were genetically heterogeneous.
It has been studded that combustion and the production of air pollution of anthracite - bituminous coal blend In a fluidized bed coal combustor, The objects of thIns study were to investigate mixing characteristics of the particles as well as the combustibility of the low grade domestic anthracite coal and Imported h19h calorific bltununous coal in the fluidized bed coal combustor. They were used as coal samples ; the domestic low grade anthracite coal with heating value of 2,010kca1/kg and the Imported high grade bituminous coal with beating value of 6,520kca1/kg. Also, the effects of air flow rate and anthracite fraction on the reaching time of steady state condition have been studied. The experimental results are presented as follows. The time of reaching to steady state was affected by the temperature variation. The steady state time was about 120 minute at 300sc1h which was the fastest. It has been found that $O^2$ and $CO^2$ concentration were reached steady state at about 100 minute. It has been found that $O^2$ concentration decreased and $CO^2$ concentration increased as the height of fluidlzed bed Increased. It was found that splash zone was mainly located from 25cm to 35cm above distributor. Also, as anthracite traction Increased, the mass of elutrlatlon particles Increased, and $CO^2$ concentration decreased. As gk flow rate Increased,$O^2$ concentration decreased and $CO^2$ concentration increased. Regardless of anthracite fraction and flow rate, the uncombustible weight percentage according to average diameter of elutriation particles were approldmately high In the case of One Particles. As anthracite traction and k now rate Increased, elutriation ratio Increased. As anthracite fraction was increased, exit combustible content over feeding combustible content was Increased. Regardless of anthracite fraction, size distribution of Ued material from discharge was almost constant. Over bed temperature 85$0^{\circ}C$ and excess air 20% , the difference of combution efficiencies were little. It is estimate that the combustion condition In anthracite-bituminous coal blend combustion is suitable at the velocity 0.3m/s, bed temperature 85$0^{\circ}C$, the excess air 20%.
Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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2016.05a
/
pp.554-557
/
2016
Recently, supercomputer has been increasingly adopted as a computing environment for scientific simulation as well as education, healthcare and national defence. Especially, supercomputing system with heterogeneous computing resources is gaining resurgence of interest as a next-generation problem solving environment, allowing theoretical and/or experimental research in various fields to be free of time and spatial limits. However, traditional supercomputing services have only been handled through a simple form of command-line based console, which leads to the critical limit of accessibility and usability of heterogeneous computing resources. To address this problem, in this paper, we provide the design and implementation of web-based HPC (High Performance Computing) job management framework for computational scientific simulation. The proposed framework has highly extensible design principles, providing the abstraction interfaces of job scheduler (as well as bundle scheduler plug-ins for LoadLeveler, Sun Grid Engine, OpenPBS scheduler) in order to easily incorporate the broad spectrum of heterogeneous computing resources such as cluster, computing cloud and grid. We also present the detailed specification of HTTP standard based RESTful endpoints, which manage simulation job's life-cycles such as job creation, submission, control and status monitoring, etc., enabling various 3rd-party applications to be newly created on top of the proposed framework.
Kim, Eun Ky;Lee, Ji Seon;Cheong, Hae Il;Chung, Sung Soo;Kwak, Soo Heon;Park, Kyong Soo
Genomics & Informatics
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v.12
no.4
/
pp.240-246
/
2014
Mutation in HNF1B, the hepatocyte nuclear factor-$1{\beta}$ (HNF-$1{\beta}$) gene, results in maturity-onset diabetes of the young (MODY) 5, which is characterized by gradual impairment of insulin secretion. However, the functional role of HNF-$1{\beta}$ in insulin secretion and glucose metabolism is not fully understood. We identified a family with early-onset diabetes that fulfilled the criteria of MODY. Sanger sequencing revealed that a heterozygous P159L (CCT to CTT in codon 159 in the DNA-binding domain) mutation in HNF1B was segregated according to the affected status. To investigate the functional consequences of this HNF1B mutation, we generated a P159L HNF1B construct. The wild-type and mutant HNF1B constructs were transfected into COS-7 cells in the presence of the promoter sequence of human glucose transporter type 2 (GLUT2). The luciferase reporter assay revealed that P159L HNF1B had decreased transcriptional activity compared to wild-type (p < 0.05). Electrophoretic mobility shift assay showed reduced DNA binding activity of P159L HNF1B. In the MIN6 pancreatic ${\beta}$-cell line, overexpression of the P159L mutant was significantly associated with decreased mRNA levels of GLUT2 compared to wild-type (p < 0.05). However, INS expression was not different between the wild-type and mutant HNF1B constructs. These findings suggests that the impaired insulin secretion in this family with the P159L HNF1B mutation may be related to altered GLUT2 expression in ${\beta}$-cells rather than decreased insulin gene expression. In conclusion, we have identified a Korean family with an HNF1B mutation and characterized its effect on the pathogenesis of diabetes.
The effects of the rice weevils(Sitophilus oryzae L.) on naked barley and wheat were studied in connection with the moisture contents and the molds in the grain under the controlled conditions; R.H. $75\%,\; 28^{\circ}C.$ 1. The moisture contents of control grain were decreased $2.07\%$ for naked barley and $1.29\%$ in wheat in four weeks. 2. The moisture contents of naked barley which had been infested with 100 weevils were decreased $0.29\%$ and were increased $0.79\% in the berley infested with 200 weevils at t beginning. In wheat, the moisture contents were decreased by$0.84\%\; and\; 0.13\%$ in respective experimental lots. 3. The moisture contents of grains have close relation with the population densities of the weevils in the grain. 4. The pattern of the change in the moisture content of grain have close relation with the population densities of the weevils in the grain. 5. The number of the mold colonies in the grain increased exponentially with the increase in the population densities of weevils in the grain. 6. The species of the mold found were A. restrictus and A. versicolor, which were the most abundant, and A. candidus was also found, but Ins common.
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