Background: Opisthorchis viverrini infection is a serious public-health problem in Southeast Asia. It is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the etiology of cholangiocarcinoma. Objectives: This study aimed to investigate Opisthorchis viverrini metacercarial infection in cyprinoid fish collected from 32 districts of Nakhon Ratchasima province, Northeastern Thailand during one year period from February 2010 to February 2011. Methods: A cross-sectional study was conducted, data being collected with pepsin-HCl digestion and stereomicroscope, respectively. Analysis was performed using SPSS Windows Version 12.0. Results: A total of 640 Cyprinidae family fish including 5 species were collected from different study sites, and investigated for O. viverrini metacercariae. The infection rate was 12.3% (79/640), predominantly in Cyclocheilichthys armatus, C. repasson, Puntioplites proctzysron, Hampala macrolepitota and Hampala dispar, respectively. The prevalence of O. viverrini metaceria in Nakhon Ratchasima area was 78.1%, predominantly in Sida and KiaKham Thale So. Conclusion: This findings stress that natural fish species in rural communities are still a source of O viverrini infection and put local people at risk, therefore public awareness and prevention campaigns are urgently required.
This paper aims to identify the class characteristics of tenants. To this end Patnaik's model is selected, because this model is most reasonable for sorting class structures of tenants. In his Model, "labor-exploitation criterion" is the main criterion for identifying class status. According to this criterion, there are five rural classes i.e. landlord, rich peasant, middle peasant, poor peasant, and full-time laborer. "Net labor ratio" is used for this purpose as empirical data handling. Net labor ratio is a ratio of net labor hired in to family labor, if hired in labor is more then this ratio is positive, and if hired out labor is more then the ratio is negative. Hired in and hired out labor includes not only direct labor but indirect labor such as labor employment or sales through rent. The results of this study are summarized as First, almost all tenants and owner cultivators are of the middle peasant class. Second, there are no rich peasant among the tenants, but 5% of owner cultivators are rich peasants, and 10% of tenants are poor peasants, owner cultivators are 1%. Third, the net: labor ratio of tenants is -0.211, and that of owners is 0.143. There are differences between tenants and owner cultivators even if land is much the same, and owner cultivators net labor ratio is positive except in the land size of 0.3-0.5ha, but that of the tenants' is negative. Fourth, proto-labor poor peasants earned 25% of income from labor, compared with under 10% of proto-tenant poor passant's. Rent to income ratio is almost 60% of proto-tenant, 27% of proto-labor among poor peasants.
Bean yellow mosaic virus (BYMV; genus Potyvirus, family Potyviridae) causes severe losses to various legume species and a number of non-legume species, particularly freesia plants. In a survey of virus diseases in Gyeonggi province, Korea, BYMV isolates were identified from many cultivated freesia species. Here, we determined the complete nucleotide sequences of a BYMV freesia isolate (BYMV-Fr; accession number FJ492961). BYMV-Fr genome consists of 9,545 nucleotides (nt) excluding the poly (A) tail and encodes 3,057 amino acid (aa), with an AUG start and UAG stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of BYMV-Fr was divided to ten proteins and the cleavage sites of each protein were determined. The coat protein (CP) and polyprotein of BYMV-Fr were compared at the aa level with those of the previously reported 4 BYMV isolates. BYMV-Fr shared 90.1 to 97.1 and 91.0 to 92.5% at the CP and polyprotein homology. Interestingly, BYMV-Fr showed identities of a lower level at the nt level of 5' noncoding region (61.4 to 67.6%) and at the aa level of P1 (71.4 to 72.8%), comparing with four BYMV isolates. Based on the aa sequence diversity of CP and polyprotein, phylogenetic analysis with the four BYMV isolates showed two distinct groups and BYMV-Fr and most BYMV isolates were most closely related to the clover yellow vein virus among 52 potyviruses. To our knowledge, this is the first report of the complete genome sequence of BYMV freesia strain.
Fassah, Dilla Mareistia;Jeong, Jin Young;Baik, Myunggi
Asian-Australasian Journal of Animal Sciences
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v.31
no.4
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pp.537-547
/
2018
Objective: This study was performed to understand transcriptional changes in the genes involved in gluconeogenesis and glycolysis pathways following castration of bulls. Methods: Twenty Korean bulls were weaned at average 3 months of age, and castrated at 6 months. Liver tissues were collected from bulls (n = 10) and steers (n = 10) of Korean cattle, and hepatic gene expression levels were measured using quantitative real-time polymerase chain reaction. We examined hepatic transcription levels of genes encoding enzymes for irreversible reactions in both gluconeogenesis and glycolysis as well as genes encoding enzymes for the utilization of several glucogenic substrates. Correlations between hepatic gene expression and carcass characteristics were performed to understand their associations. Results: Castration increased the mRNA (3.6 fold; p<0.01) and protein levels (1.4 fold; p<0.05) of pyruvate carboxylase and mitochondrial phosphoenolpyruvate carboxykinase genes (1.7 fold; p<0.05). Hepatic mRNA levels of genes encoding the glycolysis enzymes were not changed by castration. Castration increased mRNA levels of both lactate dehydrogenase A (1.5 fold; p<0.05) and lactate dehydrogenase B (2.2 fold; p<0.01) genes for lactate utilization. Castration increased mRNA levels of glycerol kinase (2.7 fold; p<0.05) and glycerol-3-phosphate dehydrogenase 1 (1.5 fold; p<0.05) genes for glycerol utilization. Castration also increased mRNA levels of propionyl-CoA carboxylase beta (mitochondrial) (3.5 fold; p<0.01) and acyl-CoA synthetase short chain family member 3 (1.3 fold; p = 0.06) genes for propionate incorporation. Conclusion: Castration increases transcription levels of critical genes coding for enzymes involved in irreversible gluconeogenesis reactions from pyruvate to glucose and enzymes responsible for incorporation of glucogenic substrates including lactate, glycerol, and propionate. Hepatic gluconeogenic gene expression levels were associated with intramuscular fat deposition.
The big gap between efficacy of population level prevention and expectations due to heterogeneity and complexity of cancer etiologic factors calls for selective yet personalized interventions based on effective risk assessment. This paper documents our research protocol aimed at refining and validating a two-stage and web-based cancer risk assessment tool, from a tentative one in use by an ongoing project, capable of identifying individuals at elevated risk for one or more types of the 80% leading cancers in rural China with adequate sensitivity and specificity and featuring low cost, easy application and cultural and technical sensitivity for farmers and village doctors. The protocol adopted a modified population-based case control design using 72, 000 non-patients as controls, 2, 200 cancer patients as cases, and another 600 patients as cases for external validation. Factors taken into account comprised 8 domains including diet and nutrition, risk behaviors, family history, precancerous diseases, related medical procedures, exposure to environment hazards, mood and feelings, physical activities and anthropologic and biologic factors. Modeling stresses explored various methodologies like empirical analysis, logistic regression, neuro-network analysis, decision theory and both internal and external validation using concordance statistics, predictive values, etc..
This study aims to explore the difference in life satisfaction and daily activities of elderly solitaries through a survey conducted from May to June 2004 to 152 who hare moi the criteria set forth for this study. Used for the survey were such tools as 11 items of questions concerning characters of the subjects, Life Satisfaction and Daily activities(K-ADL). The outcomes could be summarized as follows: 1. The majority of 152 subjects are females, aged $70{\sim}79$, living in the urban areas, non-educated with miscellaneous religions(including non-believers), jobless, self-dependent (or living expenses, without leisure, with disease, suffering from sickness despite of treatment and, thus not self-confident in health. 2. The Life Satisfaction and K-ADL of all subjects appear in general to be good with $20.03{\pm}9.74$ and $8.25{\pm}2.74$, respectively. 3. The difference in Life Satisfaction by characters of the subjects is found significantly high in those who are females, living in rural area, educated, with religions and jobs, have diseases treated and currently confident in health. 4. The difference in K-ADL by characters of the subjects is significantly high for those who are older than 80, Buddhists, jobless, affordable with living expenses by themselves, sick currently and not confident in health. Thus, it is thought vastly important to help the elderly solitaries to realize quality of life by social (including family) assistance, economic self-reliance and health promotion and prevention. Measures drawn from the analysis into issues of the elderly solitaries from various aspects may definitely contribute to avoiding and complimenting the causes of various issues relating to the elderly people and to assisting them to cope with the problems in the highly geriatric society to come.
Dung beetles (family Scarabaeidae) are one of the largest families of beetles worldwide. Due to biological behavior of these arthropods, they are considered to play an important role in the life cycle of some helminths. In the present study, dung beetles collected from cattle pastures in rural areas of Ardabil province, north-west of Iran were examined for infection with larval stages of helminths. According to the results, nematodes of 2 genera were identified including Rhabditis and Gongylonema. The more common species was Rhabditis sp. which was found in 9 species of beetles. Out of 15 different species of dung beetles, Copris lunaris was the only scarabaeid to be found naturally infected with the larval stages of Gongylonema sp. Our new findings introduce C. lunaris as a potential biological vector for transmission of Gongylonema sp. to vertebrates in the surveyed region.
Kim, Young-Nam;Lee, Hye Hyun;Kang, Eun Jin;Kang, Keum Jee;Kim, Gun-Hee
Journal of the Korean Society of Food Culture
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v.29
no.6
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pp.668-676
/
2014
The purpose of this study was to determine anthropometric measurements, dietary nutrient intakes, simple food intake frequencies, and rates of obesity in Cambodian women. A total 186 women, aged 20-69 years, living in Kampong Spue province of Cambodia and having the responsibility to prepare family meals participated in this study. Height and weight were measured body mass index (BMI) was calculated, and one-day 24-hour recall and food frequency questionnaires were conducted. In total, 18.8% were underweight, 49.5% were normal weight, 13.4% were overweight, and 18.3% were obese according to the World Health Organization BMI standard for Asians. No significant difference in height by obesity level was determined ($p{\geq}0.05$). The mean energy intake was 1571.2 kcal/day and protein intake was 43.6 g/day. There were significant differences in protein, niacin, and vitamin C intakes by obesity level (p<0.05). The prevalence of subjects consuming vitamins and minerals less than 75% of Recommended Dietary Allowances was 73-99%. All Cambodian women consumed rice ${\geq}1time/day$. Approximately 50% of subjects reported ${\geq}1time/day$ intake frequency of bread, cookies, and chips. In total, 35% indicated no intake of beans or bean products. For intake frequencies of carbonated beverages and sports drinks, there was a significant difference by obesity level (p<0.05). These results may be very helpful to prepare nutrition education programs for Cambodian women.
At the preliminary survey of Seosan demonstration project for integration of family planning and maternal and child health service, 6 questions about morbid conditions of all household members were included. Definition of morbid condition used at the survey was self-conceived physical or mental infirmity which had been caused by diseases or accidents, resulting in obstacles to ordinary daily life for past 30 days. Analyzing those morbidity data, the following results were obtained. 1. The morbid person rate was 192.2 per 1,000 persons and the morbidity rate 214.2 per 1,000 persons. 2. The percentage of household which had at least one morbid person was 61.9% and average number of morbidity per one household was 1.1. 3. Analyzing the 1st morbid condition of the total 4,433 morbid persons, morbidity rate of male was 194.6 per 1,000 persons and that of female was 189.8 per 1,000 persons. 4. The highest age specific morbidity rate was that of $0{\sim}4$ years of age, and it was 304.3 per 1,000 persons. The lowest was that of $10{\sim}14$ years of age and the rate of 93.8 per 1,000 persons. 5. The following was the order of five major diseases which were classified according to 17 International Classification of Diseases. (1) Symptoms and Ill defined conditions : 17.3% (2) Disease of the Digestive system : 15.7% (3) Infections and Parasitic disease 14.3% (4) Disease of the Respiratory system : 13.7% (5) Disease of the Nervous system and sense organs : 9.4% 6. 30 leading morbid conditions were described in Table III-2 and which composed 70.6% of total morbid conditions. 7. The percentage of the morbid conditions of which duration was longer than 90 days was 40.5%. 8. Of the total 4,433 morbid persons, 4,394 morbid persons responded for the morbid condition management method. Of those, Hospital & clinic (35.3%), drug store (40.0%), herb drug store (3.6%), health center (2.2%) ware selected for the 1st place or method for management morbid condition, and 18.0% did not search for morbid condition management.
The purpose of this study was to determine the relationships among the health concern, health practice and health status of Koreans. This study utilized the data from Korean National Health Survey (KNHS) in 1992. The data consisted of random sample of 2,799 individuals (1,304 male and 1,495 females) whose ages were between 20 and 59. The data were analyzed using SAS version 6.04 and LISREL version 7.13. The analytic methods for the study were chi-square analysis and covariance structural analysis. The results of the study were as follows. (1) There were significant positive relationships between health concern level and health practice index, and between health practice index and self-perceived health status. (2) There were negative relationships between practice index and chronic illness, and between health practice index and acute illness only in female. (3) Based on the findings, the structural model of the health concern, health practice, health status and socioeconomic variables was established and then the covariance structural analysis was used. The higher educational level and economic status were, the higher the health concern was. And urban residents were much more concerned with their health than rural residents. The more persons were concerned with health, the more they did health practices. And the more the health practice was, the higher the health status was. The younger the persons were and the higher the health status of one's family was, the higher the health status was. In female, the higher the economic status was, the higher the health status was.
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