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Evaluation of Antioxidant and Antimicrobial Activities of Ethanol Extracts of Three Kinds of Strawberries

  • Seleshe, Semeneh;Lee, Jong Seok;Lee, Sarah;Lee, Hye Jin;Kim, Ga Ryun;Yeo, Joohong;Kim, Jong Yea;Kang, Suk Nam
    • Preventive Nutrition and Food Science
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    • v.22 no.3
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    • pp.203-210
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    • 2017
  • The antioxidant and antimicrobial activities of three kinds of strawberry ethanol extracts from Robus corchorifolius L. f. (RCL), Rubus parvifolius L. var. parvifolius (RPL), and Duchesnea chrysantha Miq. (DCM) were investigated. The RPL was highest (P<0.05) in phenolic, flavonoid, and anthocyanin contents. 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities of RPL and DCM extracts were higher than that of RCL (P<0.05). Hydrogen peroxide scavenging activity of RPL was high compared to DCM and RCL (P<0.05). RCL exhibited a significant (P<0.05) potent antioxidant activity in nitric oxide radical inhibition. Inhibition diameter zone (nearest mm) of extracts against the test bacteria ranged from 11.5 in RCL to 12.5 in DCM against Staphylococcus aureus, from 10.5 in RCL to 13.5 in DCM against Streptococcus pneumoniae, from 8.5 in DCM to 10.5 in RCL against Escherichia coli, and the same inhibition of 10 mm in three of the extracts against Klebsiella pneumoniae. However, there was no inhibition against fungi Aspergillus niger and Candida albicans. Three of the extracts had the same minimum inhibitory concentration values of 12.50, 12.50, and $6.25{\mu}g/mL$ against S. aureus, K. pneumoniae, and S. pneumoniae, respectively. On the other hand, MIC values of 12.50, 12.50, and $6.50{\mu}g/mL$ were recorded for RPL, DCM, and RCL against E. coli, respectively. The result of present study revealed that extracts from three kinds of strawberries could be potential candidates as antioxidant and antimicrobial sources for functional food industries.

Comparative Study of the Effective Dose from Panoramic Radiography in Dentistry Measured Using a Radiophotoluminescent Glass Dosimeter and an Optically Stimulated Luminescence Detector

  • Lee, Kyeong Hee;Kim, Myeong Seong;Kweon, Dae Cheol;Choi, Jiwon
    • Journal of the Korean Physical Society
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    • v.73 no.9
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    • pp.1377-1384
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    • 2018
  • Accurate measurement of the absorbed dose and the effective dose is required in dental panoramic radiography involving relatively low energy with a rotational X-ray tube system using long exposures. To determine the effectiveness of measuring the irradiation by using passive dosimetry, we compared the entrance skin doses by using a radiophotoluminescent glass dosimeter (RPL) and an optically stimulated luminescence detector (OSL) in a phantom model consisting of nine and 31 transverse sections. The parameters of the panoramic device were set to 80 kV, 4 mA, and 12 s in the standard program mode. The X-ray spectrum was applied in the same manner as the panoramic dose by using the SpekCalc Software. The results indicated a mass attenuation coefficient of $0.008226cm^2/g$, and an effective energy of 34 keV. The equivalent dose between the RPL and the OSL was calculated based on a product of the absorbed doses. The density of the aluminum attenuators was $2.699g/cm^3$. During the panoramic examination, tissue absorption doses with regard to the RPL were a surface dose of $75.33{\mu}Gy$ and a depth dose of $71.77{\mu}Gy$, those with regard to the OSL were surface dose of $9.2{\mu}Gy$ a depth dose of $70.39{\mu}Gy$ and a mean dose of $74.79{\mu}Gy$. The effective dose based on the International Commission on Radiological Protection Publication 103 tissue weighting factor for the RPL were $0.742{\mu}Sv$, $8.9{\mu}Sv$, $2.96{\mu}Sv$ and those for the OSL were $0.754{\mu}Sv$, $9.05{\mu}Sv$, and $3.018{\mu}Sv$ in the parotid and sublingual glands, orbit, and thyroid gland, respectively. The RPL was more effective than the OSL for measuring the absorbed radiation dose in low-energy systems with a rotational X-ray tube.

PHOTOELASTIC STRESS ANALYSIS ON THE SUPPORTING TISSUE OF MANDIBULAR DISTAL EXTENSION REMOVABLE PARTIAL DENTURE WITH VARIOUS DESIGN OF DIRECT RETAINERS (직접유지장치 설계 변화에 따른 하악 후방연장 국소의치 지지조직의 광탄성 응력분석)

  • Lee Chang-Ho;Kim Kwang-Nam;Chang Ik-Tae
    • The Journal of Korean Academy of Prosthodontics
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    • v.30 no.2
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    • pp.203-224
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    • 1992
  • The purpose of this study was to evaluate the stress distribution developed in supporting structures by distal extension removable partial denture with 4-types of direct retainer. The direct retainers examined were Akers clasp, RPI clasp, RPA clasp and RPL clasp in bilateral & unilateral free end case. 3-dimensional photoelastic stress analysis was used to record the isochromatic fringe patterns and to calculate the compressive stress at measuring points. The results were as follows. 1. In bilateral free end case, RPI clasp exhibited the similar stress distribution on distal and mesial alveolar crest but Akers clasp exhibited higher stress concentration on distal alveolar crest than mesial alveolar crest. 2. In bilateral free end case, RPA clasp and RPL clasp exhibited the similar stress distribution on distal and mesial alveolar crest and RPL clasp exhibited higher stress concentration on buccal alveolar crest than lingual alveolar crest. 3. Akers clasp produced high stress concentration on residual alveolar ridge distally, but RPI clasp, RPA clasp and RPL clasp produced even stress distribution on residual alveolar ridge. 4. Removable partial denture in unilateral free end case exerted higher stress on abutment tooth root apex than bilateral distal extension removable partial denture.

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The effect of vitamin C on the gene expression profile of sperm protamines in the male partners of couples with recurrent pregnancy loss: A randomized clinical trial

  • Hamidian, Saeideh;Talebi, Ali Reza;Fesahat, Farzaneh;Bayat, Mohammad;Mirjalili, Ali Mohammad;Ashrafzadeh, Hamid Reza;Rajabi, Mahya;Montazeri, Fateme;Babaei, Saeid
    • Clinical and Experimental Reproductive Medicine
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    • v.47 no.1
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    • pp.68-76
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    • 2020
  • Objective: Since sperm abnormalities are known to be a major reason for recurrent pregnancy loss (RPL), any defects in DNA structure and chromatin condensation can place embryos at risk in the early stage of development and implantation. As antioxidants such as vitamin C may play a protective role against the destruction of protamine genes in sperm chromatin, this study was conducted to evaluate the effects of vitamin C on chromatin and the expression of protamine genes in the male partners of couples with RPL. Methods: Twenty male partners of couples with RPL were selected as the intervention group and received vitamin C supplementation (250 mg daily for 3 months). Healthy fertile men (n = 20) were included as controls. Sperm chromatin, DNA integrity, and the expression levels of protamine genes were evaluated before and after treatment. Results: Significant differences were found in sperm morphology, protamine deficiency, and apoptosis between the two groups and before and after vitamin C administration. A significant change was found in mRNA levels of PRM1, PRM2, and the PRM1/PRM2 ratio after treatment. Conclusion: Daily oral administration of vitamin C may improve human sperm parameters and DNA integrity by increasing protamine gene expression levels in the male partners of couples with RPL. The beneficial effects of vitamin C supplementation as an antioxidant for the male partners of couples with RPL could lead to improved pregnancy outcomes in these cases.

Association of Three Polymorphisms in Porcine Ribosomal protein L27a (RPL27A) Gene with Meat-quality Traits

  • Cho, Eun-Seok;Jeon, Hyeon-Jeong;Lee, Si-Woo;Raveendar, Sebastian;Jang, Gul-Won;Kim, Tae-Hun;Lee, Kyung-Tai
    • Journal of Animal Science and Technology
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    • v.55 no.6
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    • pp.509-513
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    • 2013
  • We identified molecular markers associated with meat-quality traits in the porcine RPL27A (ribosomal protein L27a) gene. Three single nucleotide polymorphisms (SNPs) were discovered in the porcine RPL27A gene: g.920T>C, g.1013T>C, and g.1046T>C. The g.920 T>C SNP was significantly associated with pH24 (P < 0.05) and collagen (P < 0.05), while the g.1013T>C and g.1046T>C SNPs were significantly associated with moisture (P < 0.05). Either the TTT or CCC haplotype was significantly associated with moisture, pH24 and collagen (P < 0.05, respectively). The genotypes of RPL27A associated with meat-quality traits were all located in intron 2. The three SNPs of the RPL27A found in this study will provide useful information for genetic characterization or association studies of meat-quality traits in other populations. Additionally, these markers could potentially be applied in pig breeding programs to improve meat-quality traits after validation in other populations.

Two Solutions for Unnecessary Path Update Problem in Multi-Sink Based IoT Networks (멀티 싱크 기반 IoT 네트워크에서 불필요한 경로 업데이트 문제와 두 가지 해결 기법)

  • Lee, Sungwon;Kang, Hyunwoo;Yoo, Hongsoek;Jeong, Yonghwan;Kim, Dongkyun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.12
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    • pp.2450-2460
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    • 2015
  • Recently, as interest in IoT (Internet of Things) increase, research and standardization of a new protocol which reflects the characteristics of IoT has progressed. Among them, RPL(IPv6 for Low-Power Lossy Network) is a standardized routing protocol for IoT. RPL utilizes DIO (DODAG Information Object) messages which is flooded from the sink node to the whole network for path establish and maintenance. However, in large scale networks, not only a long time is required to propagate the DIO message to the whole networks but also a bottleneck effect around the sink node is occurred. Multi-sink based approaches which take advantage of reducing routing overhead and bottleneck effect are widely used to solve these problems. In this paper, we define 'unnecessary path update problems' that may arise when applying the RPL protocol to the multi sink based IoT networks and propose two methods namely Routing Metric based Path Update Decision method and Immediate Successor based Path Update Decision method for selective routing update.

Design and Implementation of a Network Computing System for Diskless Client PCs (하드디스크 없는 클라이언트 PC를 위한 네트워크 컴퓨팅 시스템의 설계 및 구현)

  • 정연기;이광진
    • Journal of Korea Multimedia Society
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    • v.2 no.4
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    • pp.459-467
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    • 1999
  • With fully harnessing the functions provided by Windows NT, we suggest the generating method of RPL profile and the RPL protocol for remote booting on DL-NCS(Network Computing System for Diskless client PCs). This system can offer clients, without having hard disk, boot remotely Window 9x stored in server's hard disk and can share peripheral devices and application programs in the server side. For booting Window 9x from DL-NCS, twenty-five clients' PCs were set for simultaneous on and finally the PCs were booted in similar time interval compared to that from a desktop PC having local disk. This system was also experimented to the application programs. And this system can yield excellent networking environments. In sum, with using this DL-NCS, we can reduce the installing costs of peripheral device, recycle the lower grade PCs such as 486SX/DX, and save the budgets for software purchase.

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Shrinkage Structure of Ridge Partial Least Squares Regression

  • Kim, Jong-Duk
    • Journal of the Korean Data and Information Science Society
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    • v.18 no.2
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    • pp.327-344
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    • 2007
  • Ridge partial least squares regression (RPLS) is a regression method which can be obtained by combining ridge regression and partial least squares regression and is intended to provide better predictive ability and less sensitive to overfitting. In this paper, explicit expressions for the shrinkage factor of RPLS are developed. The structure of the shrinkage factor is explored and compared with those of other biased regression methods, such as ridge regression, principal component regression, ridge principal component regression, and partial least squares regression using a near infrared data set.

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The Effect of Rheum palmatum L. and Rheum undulatum L. on Rat Thoracic Aorta and Abdominal Aorta

  • Kim, Tack;Kim, Hyung-Hwan;Ahn, Duk-Kyun;Choi, Ho-Young
    • The Journal of Korean Medicine
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    • v.24 no.4
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    • pp.87-91
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    • 2003
  • Objectives: To examine the relaxational response to the water extract of Rheum palmatum L. and Rheum undulatum L. on rat thoracic aorta and abdominal aorta. Methods: Segments of thoracic aorta and abdominal aorta obtained from rats immediately after delivery were mounted in organ baths superfused on a polygraph. Results : We found that the thoracic aorta segments responded to the water extract of genus Rheum with a dose-dependent vasorelaxation. At $10^{-4}$ M 5-HT, the maximal contraction force was 93.5% of the maximum KCI-response. The 5-HT induced contractions at $10^{-4}$ M were inhibited by 86.4% and 62.1 % after addition of the high concentrations of R. palmatum root (RPR) and leaf (RPL) and R. undulatum root (RUR) and leaf (RUL). At 10 mg/ml RPR and RUR, the relaxational response at thoracic aorta and abdominal aorta with and without endothelium were 86.4%, 83.2%, 85.8%, and 62.1% of the maximum 5-HT induced contraction. Conclusion: Our result showed that RPL and RUL induced dose-dependent vasorelaxation on rat thoracic aorta and abdominal aorta, and that RPL and RUL roots have more potent effects than the leaves.

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Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

  • Choe, Chang-Yong;Cho, Young-Woo;Kim, Chang-Woon;Son, Dong-Soo;Han, Jae-Hee;Kang, Da-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.14 no.5
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    • pp.265-272
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    • 2010
  • Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and $\geq25mm$). These follicles had granulosa cell layer and theca interna and the hormone $17{\beta}$-estradiol ($E_2$)/ progesterone ($P_4$) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using $GeneFishing^{TM}$ DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.