• Applied Biological Chemistry
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• v.47 no.2
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• pp.182-186
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• 2004

Morphological observation of roots and molecular technique were used to investigate the symbiotic relationships between arbuscular mycorrhizal (AM) fungi and ginseng roots. Korean ginseng, Panax ginseng, was collected from 8 sites in Korea. Colonization pattern of AM fungi in ginseng roots was determined as an Arum type under light microscopes. Nested PCR using AM fungal specific primers was employed to amplify a partial region on 18s rDNA of AM fungi from the root extracted mixed DNA. The amplified DNA was cloned and analyzed by random fragment length polymorphism (RFLP) with restriction enzymes, AluI, HinfI and AsuC21. One from each RFLP pattern was selected for sequencing. A total 16 clones were sequenced and identified as 2 species of AM fungi; Paraglomus brasilianum and Glomus spurcum. Paramglomus brasilianum was found from most of the ginseng roots, in this syudy suggesting that this species of AM fungi could have specific relationship with the ginseng root. Possible roles of AM fungal species in ginseng roots are discussed.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.15 no.2
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• pp.102-110
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• 2015

The main source of noise in computed tomography (CT) images is a quantum noise, which results from statistical fluctuations of X-ray quanta reaching the detector. This paper proposes a neural network (NN) based hybrid filter for removing quantum noise. The proposed filter consists of bilateral filters (BFs), a single or multiple neural edge enhancer(s) (NEE), and a neural filter (NF) to combine them. The BFs take into account the difference in value from the neighbors, to preserve edges while smoothing. The NEE is used to clearly enhance the desired edges from noisy images. The NF acts like a fusion operator, and attempts to construct an enhanced output image. Several measurements are used to evaluate the image quality, like the root mean square error (RMSE), the improvement in signal to noise ratio (ISNR), the standard deviation ratio (MSR), and the contrast to noise ratio (CNR). Also, the modulation transfer function (MTF) is used as a means of determining how well the edge structure is preserved. In terms of all those measurements and means, the proposed filter shows better performance than the guided filter, and the nonlocal means (NLM) filter. In addition, there is no severe restriction to select the number of inputs for the fusion operator differently from the neuro-fuzzy system. Therefore, without concerning too much about the filter selection for fusion, one could apply the proposed hybrid filter to various images with different modalities, once the corresponding noise characteristics are explored.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.123-128
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• 1993

Genes for dinitrogenase reductase (nifH) and dinitogenase a subunit (nifD) were found to be located on 7.9 kb of EcoRI, 6.5 kb of Sail, 7.3 kb of HindlII and 4.4 kb of Pstl fragments of the genomic blot of Rhizobium sp. SNU003. a symbiotic strain from root nodule of Canavalia lineata. Nine recombinant phage nif-clones were selected from the genomic library constructed by using EMBL-3 BamHI arms of bacteriophage lambda. Among them. Rnif-6 had insert DNA of 15.3 kb. in which 7.6 kb of BamHI!SacI fragment contained nifHD region. Therefore, the 7.6 kb fragment was subcloned into pUC19 and partial restriction map was constructed. As the results, nifH and nifD were found to be located continuously on 4.5 kb of BamHI/BglIl in the genome of Rhizobium sp. SNU003 strain.

• Journal of Environmental Science International
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• v.25 no.6
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• pp.889-895
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• 2016

In Danseong-myeon (myeon is a subdivision of), Sancheong-gun, and Sugok-myeon, Jinju City, pot seedling culture was used at the rates of 100% and 62%, respectively. Root restriction seedling culture was used relatively more frequently than pot seedling culture in Yangchon-myeon, Nonsan City, Guryong-myeon, Buyeo-gun, and Wolsan-myeon, Damyang-gun. Open-field seedling culture was used at the rates of 80% and 54% in Samnangjin-eup (eup is a subdivision of a gun), Miryang City and Ssangnim-myeon, Goryeong-gun, respectively. In Danseong, Sancheong, and Sugok, Jinju, granite soil was used as the medium for pot seedling culture at the rates of 90% and 80%, respectively. In Yangchon, Nonsan, Wolsan, Damyang, and Ssangnim, Goryeong, commercial bed soil and coir dust were used for seedling culture at the rates of 73%, 64%, and 60%, respectively. At the main production sites, the use of granite soil for seedling culture, highest rate in 2000-2005, continued to decrease; in 2011-2013, granite soil was used at the rate of 37%, while commercial bed soil and coir dust were used at the rates of 32% and 14%, respectively. The bulk density of commercial bed soil, expanded rice hulls, and coir dust was $0.11-0.16g/cm^{-3}$. Coir dust was the lightest material but had the highest and, 36.7% and 21.8%, respectively. In sandy loam soil and granite soil was low. The pH of all growth media was between 5.4 and 7.0, and the values were in the range of $0.15-0.66dS{\cdot}m^{-1}$. However it was necessary, to adjust the pH levels and enhance the of expanded rice hulls, sandy loam soil, and granite soil. Similarly, the fertilizer concentration and air permeability of coir dust need to be adjusted.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.13 no.2
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• pp.199-202
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• 1991

Chin is located on most prominent portion of the face, so make more important esthetic value in impression. So historicaly, many authors had made efforts on correction of deformed chin, termed genioplasty. But those technique have been performed on limited cases, which have normal shape or width of chin. In the cases with more narrow or short chin, location of mental foramen or root of incisors restrict the use of such technique. But modified genioplasty technique we used can be performed without any restriction and change of frontal profile of chin. So we report a case that was performed with modified horizontal step osteotomy for advancement genioplasty, which had been reported by Hinds and Kent, with some complications.

• Korean Journal of Microbiology
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• v.30 no.1
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• pp.30-36
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• 1992

nif (nitrogen fixation)-H.D, K genes of Frankia sp. SNU 014201. a symbiotic strain isolated from root nodule of Alnus hirsura, were found to be located in the genome on 13.5 kb of EcoRI, 18.0 kb of BamHI, 10.5 kb of BglII and 4.5 kb of KpnI fragments. Using EMBL-3 BamHI arms of bacteriophage lambda. the genomic library was constructed. from which fourteen recombinant phage nif-clones were selected. Among them, Ahnif-I2 had insert DNA of 18 kb, in which 7.9 kb of BamHl fragment contained nif-H, D, K and 3.6 kb of HindlIl/KpnI had nif-H and partial -D. Therefore, the 7.9 kb and 3.6 kb fragments were subcloned and partial restriction maps were constructed. As the results, nif-F1, D.K genes were found to be located continuously on the 6.5 kb of HindII/BamHI and 5.2 kb of SalIIBamHI fragment in the genome of Frankia sp. SNU 014201.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.193-199
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• 2006

Halotolerant spore-forming Gram-positive bacteria were isolated from the root, rhizosphere, and non-rhizosphere soil of Blutaparon portulacoides. The different isolates were characterized genetically using an amplified ribosomal DNA restriction analysis (ARDRA), and phenotypically based on their colonial morphology, physiology, and nutritional requirements. Three different 16S rRNA gene-based genotypes were observed at a 100% similarity using the enzymes HinfI, MspI, and RsaI, and the phenotypic results also followed the ARDRA groupings. Selected strains, representing the different ARDRA groups, were analyzed by 16S rDNA sequencing, and members of the genera Halobaeillus, Virgibacillus, and Oceanobacillus were found. Two isolates showed low 16S rDNA sequence similarities with the closest related species of Halobacillus, indicating the presence of new species among the isolates. The majority of the strains isolated in this study seemed to belong to the species O. iheyensis and were compared using an AP-PCR to determine whether they had a clonal origin or not. Different patterns allowed the grouping of the strains according to Pearson's coefficient, and the resulting dendrogram revealed the formation of two main clusters, denoted as A and B. All the strains isolated from the soil were grouped into cluster A, whereas cluster B was exclusively composed of the strains associated with the B. portulacoides roots. This is the first report on the isolation and characterization of halotolerant spore-forming Gram-positive bacteria that coexist with B. portulacoides. As such, these new strains may be a potential source for the discovery of bioactive compounds with industrial value.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.452-457
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• 1998

Thirty-eight isolates of Phytophthora sp. caused rots on roots and basal stems were collected from five flowering plants from 1992 to 1997 at eight cultivation areas in Korea. All the isolates were identified as P. nicotianae based on following characteristics. The fungus produced markedly papillate, not caducous and ovoid to spherical sporangia, abundant chlamydospores, and small oospores with amphigynous antheridia only when paired with either A1 or A2 mating type. All isolates grew well at 35$^{\circ}C$ and showed distinct arachnoid colony patterns on CMA and PDA. Sizes of sporangia and chlamydospores of five representative isolates from each plant averaged 43-52$\times$30-38 ${\mu}{\textrm}{m}$ and 28 ~34 ${\mu}{\textrm}{m}$. Mating type of the isolates was either A1 or A2, and oogonia and oospores were measured as 28~31 ${\mu}{\textrm}{m}$ and 21~25 ${\mu}{\textrm}{m}$. PCR-RFLP analysis of rDNA of the five isolates resulted that restriction band patterns of the small subunit and ITS regions were identical to a perilla isolate of P. nicotianae, but distinct from P. cactorum and P. capsici. Cross inoculation tests showed that the five isolates had pathogenicity to lily, christmas cactus, anthurium, baby's breath and carnation with different degrees. However, each isolate showed stronger pathogenicity to its corresponding original host than others. Among five lily cultivars Georgia and Quririna were more susceptible than Napoli and others. This is first report of Phytophthora root and stem rot of lily, Christmas cactus, anthurium, baby's breath and monochoria in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.285-292
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• 1997

This study was conducted to obtain the transgenic Brnssica napus plants with tobacco Apase gene using the binary vector system of Agrobacteriurn fumefociens. The results obtained were summarized as follows: A repressible acid phosphatase gene of Saccharon~yces cerevisiae, pho105 was used for screening of tobacco Apase cDNA. In order to identify Apase gene in tobacco genome, Southern blot analysis was pcrformed and the Apase gcnc may be present as a single copy, or at most two or three copies, in tobacco genome. To isolate the tobacco Apase gene, tobacco cDNA library was constructed using purifed mRNA from -Pi treated tobacco root and the plaque forming unit of the library was 2.8 x $10^5$ pfu/m${\ell}$, therefore the library might cover all expressed mRNAs. Using pho5 as a probe. tobacco Apase cDNA was cloned, and restriction mapping and Southern blot analysis of cDNA insert were revealed that the 3.6 kb cDNA contained tobacco acid phosphatase cDNA. Plasmid pGA695 -tcAPl was constructed by subcloning tobacco Apase cDNA into the Hind site of pGA695 with 35s promoter which can be expressed constitutively in plants. The Brassica napus cotyledonary petioles were cocultivated with the ,4 grobacteriunz and transferred to the selection medium. The transformed and regenerated plants were transplanted to soil medium. Southern blot analysis was done on the transformed plants, and it was confirmed that a foregin gene was stably integrated into the genonies of B. nnpus plants.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.16 no.10
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• pp.941-950
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• 1991

channel is severely degraded by multipath delay time spread. In this paper. We propose a simple multipath delay time detection method, which has a merit of in serviceable, yet simple H/W realizability for $\pi/4$ shift QPSK by detecting cross channel interference. A $\pi/4$ shift QPSK signal originally has quadrature channel(Q-ch) component. Thus in order to measure CCI between in-phase channel(I-ch) and quadrature channel(Q-ch), which closely related to multipath delay time, Frequency doubling scheme(frequency doubler) and differential detector is proposed, which makes $\pi/4$ shift QPSK signal look like BPSK and also makes it possible for CCI to be detected at I-ch detector output. To get an information from time varying I-ch output signal under the multipath lading environment, a method for obtaining the mean of the absolute value$(V_{MABS}(t))$ and another one for obtaining the root mean square value$(V_{RMS}(t))$ of CCI are proposed. Furthermore, a relationship between delay spread and CCI is also analyzed. In order to confirm theoretical results, computer simulation has been carried out under the quasi-static and Reyleigh distributed two ray multipath fading environments. A fairly good result was obtained. However it was also shown that this method is sensitive to bandwidth restriction to some extent. In addition, some idea for a simple hardware realization for the frequency doubler are given.