• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.24 no.6
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• pp.462-469
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• 2014

After drilling operations at the offshore plant, crude oil is producted under high pressure. After that time, oil recovery is reduced, because the pressure of the pipe inside is low during the secondary produce. At that time injection sea water at the pipe inside through water injection pump that the device increase to recovery. A variety of mathematical analysis during the detailed design analysis was not made through the dynamics characteristic at the domestic company. 2D model has reliability of analysis results for the uncomplicated model. Also element and the node the number of significantly less than in the 3D model. So, the temporal part is very effective. In addition, depending on the quality of mesh 3D is a real model and FEM model occurs error. So, user needs a lot of skill. In this paper, a 2D finite element analysis was performed through the dynamics analysis and the study model was validated.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.7
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• pp.605-611
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• 2011

Shimmy is a self-excited vibration in lateral and torsional directions of a landing gear during either the take-off or landing. It is caused by a couple of conditions such as a low torsional stiffness of the strut, a free-play in the landing gear, a wheel imbalance, or worn parts, and it may make the aircraft unstable. This study was performed for an analysis of the shimmy stability on a small aircraft. A nose landing gear was modeled as a linear system and characterized by state-equations which were used to analyze the stability both in the frequency and time-domain for predicting whether the shimmy occurs and investigating a good design range of the important parameters. The root-locus method and the 4th Runge-Kutta method were used for each analysis. Because the present system has a simple mechanism using a friction to reinforce the stability, the friction, a non-linear factor, was linearized by a describing function and considered in the analysis and observed the result of the instability reduction.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.6
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• pp.81-89
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• 2009

Dancer systems are typical equipment for attenuation of tension disturbances. Lately, demands for high speed roll-to-roll machines are rising but it is prior to attenuate the tension variation on the web entering into the printing zone to achieve the speed increment. Maintaining a constant tension before the first printing cylinder is the key of high speed, high quality printing. Dancer has been researched in two ways, whether it is controlled or not. The first one is active dancer and the other one is passive dancer. In the active dancer, a position of idle roll of dancer is measured and the roll is moved by external hydraulic cylinder to control tension disturbances. While the passive one composed with spring, damper and idle roll has no external actuator to position the idle roll. The tension disturbance causes movement of dancer roll and the displacement of the roll regulates the tension variation. On the other hand a composite type of dancer is applied for roll-to-roll printing machines. It has same apparatus as passive dancer. The displacement of roll is measured and front(or rear) driven roller is controlled to position the roll. In this paper, it is presented an analysis of pendulum dancer including position feedback PI control and logic for PI gain tuning in roll-to-roll machines. Pole-zero map and root locus with varying system parameters gives a design method for control of the dancer.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1995.04a
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• pp.240-245
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• 1995

제어이론의 역사적 발전사를 고찰해보면 1930년대부터 1960년대까지를 고전 제어(classical control) 시대로 분류되고 이때 주로 사용되었던 용어들은 주파수역(frequency domain)에서 사용된 개념인 극점(pole), 영점(zero), Nyquist, 근궤적(root-Locus) 선도(plot)등으로 대표된다. 그 다음단계인 현대 제어(modern control) 시대 (1960년대-1980년대)때는 새로운 개념들이 도입 되었는데 시간역(time domain)에서 사용되는 상태공간(state-space) 모델, 가제어성(controllability), 가관측성(observability), Kalman 필터, LQG 제어 등이다. 1980년대부터 현재까지를 강인제어(robust control) 시대로 분류하는데 이것의 특징들은 극점이나 영점 대신 상태공간 모델을 사용하여 주파수역에서 정의되는 개념들인 H$_{\infty}$ 합성법, .$\mu$ 해석법, LQG/LTR 및 QFT, Lyapunov 등으로 대표된다. 현대제어시대때는 제어기 K는 공칭 플랜트 모델 G$_{0}$를 기준으로 설계되었으나 실제로 공칭 플랜트 모델은 실제 플랜트와 항상 같을 수가 없었다. 따라서 실제 플랜트 G는 G=G$_{0}$ + .DELTA.G로 표현되며 여기서 .DELTA.G는 플랜트 불 확실성(plant uncertainty), 즉 실제 플랜트와 공칭 플랜트의 차이를 나타낸 다. 이 플랜트 불확실성은 제어기가 실제 응용되어 사용되었을 때 제대로 작동하지 않는 주요 이유중에 하나이다. 이와 같은 상황에서 안정도 강인성 (stability robustness) 및 성능 강인성(performance rosubtness)의 보장은 상 당히 중요한 문제로 대두되었으며 주어진 플랜트 불확실성하에서 이러한 강이성들이 보장되는 제어이론들 중 H$_{\infty}$ 제어이론이 많이 연구/응용 되고 있다. 특히 공칭 플랜트 모델과 함께 사용되는 플랜트 모델과 함께 사용되는 플랜트 불확실성 모델은 직접적으로 성능 및 안정도에 영향을 미치므로 주의 깊게 선정해야 한다.

• Molecules and Cells
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• v.40 no.10
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• pp.714-730
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• 2017

Pre-mRNA splicing further increases protein diversity acquired through evolution. The underlying driving forces for this phenomenon are unknown, especially in terms of gene expression. A rice alternatively spliced transcript detection microarray (ASDM) and RNA sequencing (RNA-Seq) were applied to differentiate the transcriptome of 4 representative organs of Oryza sativa L. cv. Ilmi: leaves, roots, 1-cm-stage panicles and young seeds at 21 days after pollination. Comparison of data obtained by microarray and RNA-Seq showed a bell-shaped distribution and a co-lineation for highly expressed genes. Transcripts were classified according to the degree of organ enrichment using a coefficient value (CV, the ratio of the standard deviation to the mean values): highly variable (CVI), variable (CVII), and constitutive (CVIII) groups. A higher index of the portion of loci with alternatively spliced transcripts in a group (IAST) value was observed for the constitutive group. Genes of the highly variable group showed the characteristics of the examined organs, and alternatively spliced transcripts tended to exhibit the same organ specificity or less organ preferences, with avoidance of 'organ distinctness'. In addition, within a locus, a tendency of higher expression was found for transcripts with a longer coding sequence (CDS), and a spliced intron was the most commonly found type of alternative splicing for an extended CDS. Thus, pre-mRNA splicing might have evolved to retain maximum functionality in terms of organ preference and multiplicity.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• The Journal of Dong Guk Oriental Medicine
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• v.5
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• pp.1-13
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• 1996

This study was done in order to investigate the protective effects of A.G.R.(Angelicae gigantis Radix) aqua-acupuncture on acetaminophen induced liver damage in rats. The liver damage was induced by acetaminophen (500mg/kg) injection into the peritoneum. The A.G.R. aqua-acupuncture solution was injected into the corresponding loci to Ganshu($BL_{18}$) and Qimen($LR_{14}$) of human body and a blank locus of the root of tail on four consecutive times at 0, 3, 6, and 12 hours after acetaminophen injection. And the serum GOT, GPT, LDH, ALP activities, total bilirubin, direct bilirubin levels were measured in the rats. The serum GOT, GPT, LDH ALP activities and bilirubin level were decreased comparing with that of a control group in case of A.G.R. aqua-acupuncture treated group, specially Ganshu and Qimen aqua-acupuncture treated groups showed an obvious significant decrease.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.12
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• pp.29-35
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• 2008

In this paper, The author present a load current feed-forward compensator by method that improve voltage controller of Step-down Chopper to get stable output voltage to sudden change of load current. To confirm the characteristics of a presented load current feed-forward compensator compared each transfer function of whole system that load current feed-forward compensator is added with transfer function of whole system that existent voltage controller is included using Mason gains formula in Root locus and Bode diagram. As a result the pole of system is improved, extreme point of the wave and system improves, and size of peak value and phase margin of break frequency in resonance frequency confirmed that is good. Therefore, presented control technique could confirm that reduce influence by perturbation and improves stationary state and dynamic characteristics in output of Step-down Chopper.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.12
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• pp.1483-1487
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• 2013

Water injection pump outputs oil with high pressure during this process, seawater is injected into the well to recover the well pressure and maintain high productivity. A water injection pump has high productivity, and therefore, it serves as a key piece of equipment in marine plants. In this light, water injection pumps are being studied widely in industry. In this study, the rotor dynamics is analyzed to determine the natural frequency according to the bearing stiffness and operation speed change. This study aims to establish the pump reliability through critical speed, stability, and unbalance response analysis.

• Journal of Plant Biotechnology
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• v.34 no.2
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• pp.139-144
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• 2007

Hypocotyl explants of Chinese cabbage (cvs. "Jeong Sang") produced transgenic calli on callus induction medium (MS salt, B5 vitamin, 5 mg/L acetosyringone, 1 mg/L 2,4-D, 3% sucrose, 400 mg/L cefotaxime, 100 mg/L paromomycin, pH 5.8) after cocultivation with strains of Agrobacterium tumefaciens (EHA101, LBA4404, GV3101) harboring the pPTN290 containing paromomycin-resistance gene as a selectable marker, and then they transferred to root induction medium (1/2MS salt, MS vitamins, 2% sucrose, 100 mg/L paromomycin, 100 mg/L cefotaxime, pH 5.8) and shoot induction medium (MS salt, B5 vitamin, 4 mg/L $AgNO_3$, 4 mg/L 6-benzyladenine, 3 mg/L alpha-naphthaleneacetic acid, 100 mg/L paromomycin, 100 mg/L cefotaxime, 3% sucrose, pH 5.8) in order. There was a significant difference in the frequency of transgenic calli depending on Agrobacterium strains. In particular, the highest frequency (6.1%) of transgenic calli was obtained from the hypocotyls cocultivated with EHA101 strains. Also, the frequency (%) of transgenic root and plants from each transgenic callus clone were obtained with 60.7% and 38.2% in EHA101, with 8.3% and 0% in LBA4404, with 20.5% and 85.7% in GV3101 strains, respectively. They were grown to maturity in a greenhouse and normally produced $T_2$ seeds. GUS histochemical assay for progeny ($T_2$) revealed that the transgenes was expressed in the plant genome, and progeny analysis from 7 independent transgenic events demonstrated that the transformants transmitted the transgene as a single or multiple functional locus.