• Journal of Korean Society of Forest Science
• /
• v.95 no.6
• /
• pp.735-742
• /
• 2006

The objective of this study was to elucidate the tolerance of woody plants to simulated acid rain in relation to mycorrhizal inoculation. Germinating seedlings of Robinia pseudoacacia were planted in 1I pots with autoclaved soil mixture of vermiculite, sand and nursery soil at 1:1:1 ratio. Each pot was inoculated with both crushed root nodules from a wild tree of the same species and commercial arbuscular mycorrhizal inoculum of Glomus intraradices at the time of planting the seedlings. Simulated acid rains at pH 2.6, 3.6, 4.6, and 5.6 were made by mixing sulfuric acid and nitric acid at 3: 1 ratio. Each pot received nutrient solution without N and P, and was also supplied with 180 ml of the one pH level of the acid rains once a week for 50 days. The plants were grown in the green house. At the end of experimental period, plants were harvested to determine contents of chlorophyll, mineral nutrients and net photosynthesis in the tissues, dry weight of the plants, and mycorrhizal infection in the roots. Mycorrhizal infection rate was significantly reduced only at pH 2.6, which meant vitality of G intraradices was inhibited at extremely low pH. Height growth, dry weight production, nodule production and chlorophyll content were increased by mycorrhizal infection in all the pH levels except pH 3.6. Particularly, mycorrhizal inoculation increased root nodule production by 85% in pH 5.6 and 45% in 4.6 treatments. But the stimulatory effect of mycorrhizal inoculation on nodule production was reduced at pH 3.6 and 2.6. Net photosynthesis was increased by mycorrhizal infection in all the pH levels. The phosphorus(P) content in the tissues was increased by 43% in average by mycorrhizal inoculation, which was statistically significant except in pH 2.6. It was concluded that mycorrhizal inoculation of Robinia pseudoacacia would enhance growth and resistance of the plants to acid rain by improving the photosynthesis, phosphorus nutrition, and more nodule production.

• Journal of Korean Society of Forest Science
• /
• v.104 no.1
• /
• pp.60-66
• /
• 2015

This study was conducted to determine the effects of seed storage method ($-20^{\circ}C$, $2^{\circ}C$ dry, $2^{\circ}C$ wet 30 days, $2^{\circ}C$ wet 60 days, stratification and room temperature) and shading treatment(control, 50%, 80%) on seed germination, seedling growth of endangered species, Iris dichotoma and Iris setosa. As a result, seed germination rate of I. dichotoma was the highest at 75% when seed were stored at $2^{\circ}C$ wet 60 days and then sown under non-shading condition. The seed of I. dichotoma belong to intermediate seed. Seed germination rate of I. setosa was the highest at 95% when seed were stored at $2^{\circ}C$ wet 60 days and then sown under 80% shading condition. The seed of I. setosa belong to recalcitrant seed. Seedlings of I. dichotoma and I. setosa showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{\circ}C$ wet 60 days under non-shading condition, with the growth characteristics of plant height (6.4, 7.2 cm), number of leaves (3, 4), leaf width (4.6, 3.2 mm), leaf length (5.7, 6.8 cm), fresh weight (aerial/root part; 144/260, 97/153 mg), dry weight (aerial/root; 31/20, 17/17 mg) and seedling vigor index and modified seedling vigor index (13,895/9,479, 13,256/8,668). In this research, I. dichotoma and I. setosa seed were stored at $2^{\circ}C$ wet 60 days, and then sown in non-shading condition, seed germination rate was more than 75%, 90%, respectively, and production of superior quality seedlings.

• The Korean Journal of Ecology
• /
• v.26 no.1
• /
• pp.39-47
• /
• 2003

The leaf aqueous extracts from five gymnosperms plant were investigated for allelopathy with five Hibiscus syriacus varieties. The leaf aqueous extract of Pinus rigida had the highest total phenolic compound of 2.21mg/L, whereas the soil under Pinus koraiensis canopy had the highest total phenolic compound of 1.38mg/L. Fourteen phenolic compounds were isolated from five gymnosperm plants by HPLC. Among them, phenolic compounds were the highest in P. rigida (320.56 g/mg) with the primary compound 5-sulfosalicylic acid (312.55 g/mg). The correlation between leaf total phenolic compound and pH was not significant, while the total phenolic compound of the leaf extract changed soil pH. The relative seed germination of H. syriacus varieties showed 25% was threshold concentration. The germination rates of varieties were similar to the control group or showed slight stimulation to treatment of P. koraiensis extract. H. syriacus Cambanha was similar to the control group or showed stimulation in all treated groups. H. syriacus Seohohyang showed stimulation in both root and shoot growth compared to the control group. In other varieties except Seohohyang, shoot growth was similar to the control group, while root growth was stimulated in all treated groups. The extracts of tested gymnosperms showed significantly more stimulation to transplanted Seohohyang seedlings, whereas others were similar to control or inhibited in the greenhouse. The dry weight of Seohohyang was greater in all treated groups than the control group, while other varieties were inhibited. All gymnosperm extracts stimulated the chlorophyll contents of Seohohyang and H. syriacus Koyoro but other varieties were not significantly affected. Accordingly, it is suggested that Seohohyang seems the most desirable when planted within these five gymnosperms.

• Journal of Bio-Environment Control
• /
• v.30 no.1
• /
• pp.10-18
• /
• 2021

Acclimatization after grafting and graft-take that in order to produce plug seedlings of high-quality are important plug seedling stage which not reduce the plug seedlings quality before shipment. Appropriate acclimatization environment can not only increase seedling quality before secondary growth period, but also effective in promoting the growth of plug seedlings. This study was conducted to determine the optimal the shading period and shading level for acclimatization of tomato and pepper grafted plug seedlings after graft-take. The tomato and pepper seedlings were used in this experiment, and after graft-take, a tunnel was installed on a bed in glasshouse of venlo type to started shading treatment. The shading levels were 35, 55, 75 and 95%, and the shading periods were 1 and 2 weeks, and non-treatment was set as the control. In the case of tomato grafted plug seedling, plant height, stem diameter, dry weight of root, leaf area were significantly higher at the shading period of 1 week and the shading level was 55%. In the case of pepper grafted plug seedling, plant height, stem diameter, and leaf area were the highest in the shading period of 2 weeks and the shading level was 35%. However, dry weight of root, compactness, and T/R ratio, which seedling quality indicators, were lower than in the shading period of 1 week and the shading level 55%. Therefore, considering the quality of seedlings, it is suggested that shading period of 1 week with shading level of 55% treatment can be suitable to produce high quality grafted plug seedlings of tomato and pepper.

• Journal of Bio-Environment Control
• /
• v.29 no.2
• /
• pp.130-140
• /
• 2020

This study was conducted in order to closely examine about optimum shading for superior seedling production a container seedling of Tetradium daniellii, which is being increased the demand for a seedling due to being used for alternative energy, ecological restoration and honey plant. The experiment of investigating the optimum shading on T. daniellii was carried out by using plastic container types (350 ml/cavity) for the forestry facility cultivation. The shading level was treated with full sunlight and with 35%, 55%, 75% of the full sunlight. As a result of having surveyed height and root collar diameter growth of a containerized seedling in T. daniellii, a case of the shading experiment showed a noticeably high value was indicated in the full sunlight. It was surveyed that the stronger shading level leads to the lower growth value. Root development was most active in full sunlight. Dry matter production, it was investigated to be the highest in full sunlight. It was surveyed to be the similar tendency to the outcome of height and root collar diameter growth. QI, which is index of showing the quality of a seedling, stood at 0.98 in full sunlight, thereby having been investigated to be the highest. As for the chlorophyll content in a seedling, the highest chlorophyll content was indicated in the 75% shading treatment with the relatively highest shading level. The photosynthetic rate and the water use efficiency were surveyed to be the highest in full sunlight with 8.48 μmolCO₂·m^-2s^-1, 1.40 μmolCO₂·mmolm^-1H₂O, respectively. As a result of surveying the whole experiment, optimum shading level for superior seedling production a container seedling of T. daniellii is determined in full sunlight (0%). It is expected that this will be used as a basic data for mass production.

• Journal of Korean Society of Forest Science
• /
• v.11 no.1
• /
• pp.1-24
• /
• 1970

This experiment was carried out to elucidate the behavior of nutrition absorption and the acid tolerance, on varying soil pH levels, of Pinus thunbergii, Pinus densiflora, Pinus rigida, PInus koraiensis, Larix leptolepis and Abies holophylla which are 6 month old seedlings through examinations of the absorption behavior, the acid and alkali tolerance of N,P,K, Ca and Fe in the sand culture media with artificially controlled pH. 1. The increase rate of dry matter conditioned by the behavior of nutrition absorption in a strong acidic condition (pH3) was found in the following decreasing order of Pinus rigida, Pinus thunbergii. This order, therefore, can also be said to apply to the case of acid tolerance. 2. The increase rate of dry matter in a strong alkaline condition (pH9) was found in the following decreasing order of Pinus thunbergii, Pinus rigida, Pinus densiflora, Pinus koraiensis, Abies hoplohylla, Larix leptolepis. This order, therefore can be said to apply to the case of alkali tolerance. 3. Considering the absorption behavior of K and Ca, it is possible to decide the tolerance of acid from the absorption behavior and it can be said that if the absorption quantity is K>Ca the acid tolerance is strong and vice versa. 4. Considering the ratio of K:Fe and Ca:Fe in this experiment, a strong acid tolerance shows a higher ratio of K/Fe and vice versa. And also a strong acid tolerance shows a lower ratio of Ca/Fe and vice versa. It is therefore possible to decide the acid tolerance from the absorption behavior of K, Ca and Fe. 5. Of the tree species subjected to this experiment, Pinus thunbergii was the strongest in both acid and alkali tolerance. 6. In spite of that in all species the dry weight in the root is smailer than that in the top, the quantity of Fe contained in the root was larger than that in the top.

• The Korean Journal of Pesticide Science
• /
• v.14 no.4
• /
• pp.407-414
• /
• 2010

Bacillus subtilis strains isolated from different regions of Korea were screened for their plant growth promotion and induced systemic resistance (ISR) in tomato and red-pepper. The plant growth promotion on red-pepper and tomato revealed maximum plant height (22.73 cm) on red pepper treated with B. subtilis strain JE 21-1 and 30.18cm in case of tomato treated with B. subtilis strain JE 8-1. There was also significant improvement in root and shoot dry weight in both the plants. The strain JE 21-1 showed better promise for all growth parameters in red-pepper and tomato when compared to other strains and positive check BTH. Different strains screened in square plate method also revealed maximum plant height and leaf width, and suppressed anthracnose on red pepper in case of strain JE 21-1 at $10^6$ and $10^7$ cells/ml when compared to other strains. In all the bacterial inoculations the population was significantly high when compared to untreated check. In plant growth promotion with respect to fruit length and weight, fruit length was maximal in treating with JE 9-4 and ES 2-2, while fruit weight was maximal in treating with JE 3-6, ES4-2, ES2-2 and JE 21-2 on red pepper. In case of tomato, comparatively better fruit weight was in JE 21-1, ES 3-3 and JE 10-2 when compared to BTH and untreated control. The soft rot disease caused by Pectobacterium carotovorum SCCI was completely suppressed in case of transgenic tobacco harboring GUS gene related to PR1a and increased the level of salicylic acid significantly in combined application of JE 9-4 on par with BTH. Thus, this study clarified some potential Bacillus subtilis strains for plant growth promotion and ISR in red-pepper and tomato.

• Journal of Bio-Environment Control
• /
• v.26 no.3
• /
• pp.227-234
• /
• 2017

This study aimed to investigate the nutrient solution developed by based on nutrient-water absorption rate of strawberry 'Maehyang' by comparing growth and yield for 8 months with 5 kinds of nutrient solution with different ion composition. Strawberry plants were planted at elevated bed and supplied with five kinds of nutrient solutions (RDA), Yamazaki, PBG, University of Seoul (UOS) and NewUOS from one month onwards. Five types of nutrient solution were supplied to the strawberry plants associated with EC $1.0dS{\cdot}m^{-1}$, pH 6.0, $150{\sim}300mL{\cdot}plant^{-1}$ per day. At 60 days after planting, leaf width and leaf petiole of the strawberry plants showed significant differences among nutrient solution types and photosynthesis was higher in RDA and NewUOS nutrient solution and lower in PBG nutrient solution. The EC of the drainage on vegetative growth stage was $0.7{\sim}0.8dS{\cdot}m^{-1}$, which is lower than the supplied EC level, and to $1.0-1.2dS{\cdot}m^{-1}$, afterwards. The pH of the drainage was higher in Yamzaki solution as 6.2~6.8, while the pH of the UOS nutrient solution was lower in 5.1~5.2. Nitrate content was most absorbed in vegetative growth stage and after flower clusters development. The potassium uptake was highest at the NewUOS followed by UOS and Yamazaki nutrient solution. At six months after -planting fresh weight and dry weight of shoot and root were higher in UOS and NewUOS nutrient solution than other nutrient solutions, and the dry matter ratio was lower at 43.5% in Yamazaki nutrient solution and 30.6% in NewUOS nutrient solution than other solutions. Length, width, weight, and sugar content of the strawberries harvested from December to February were unaffected by treatment, but yield was higher in NewUOS nutrient solution due to increasing fruit number and average weight. From March to May, number of fruit was higher in Yamazaki nutrient solution. In conclusion, there was no difference in the growth of 'Maehyang' when 5 nutrient solutions were grown under hydroponics. But in order to improve the marketability, the NewUOS nutrient solution is appropriate to use from planting to February and it is suitable to use Yamazaki nutrient solution after March when temperature is high and the amount of fruit set per inflorescence.

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.4
• /
• pp.294-300
• /
• 1996

We performed an experiment to confirm the possibility of wasabi culture using the wat­er of the Soyang River Dam in Chunchon and the ground water in Suwon. Water mineral content of Soyang River except for P was less than that of ground water of Suwon. Dis­solved oxgen and E C of Chunchon was proper to culture wasabi but E C and dissolved oxgen of Suwon was not suitable for that. Water temp. of Soyang river was very changable by month while that of the ground water in Suwon was kept constantly. In Soyang river of Chunchon the month that water temp. show $8{\sim}18^{\circ}C$, optimal growth temp., was May to Nov. and the month that water temp. show less than $6^{\circ}C$, growth limit temp., was $Jan.\;{\sim}\;Feb.$ of Chunchon. Rhizome weight of main stem in Chunchon and Suwon was 63g and 22g per plant and rate of maketable rhizome was each 80%, 0% by culture of 32 months to include raising see­dling period of 13 months. Dry matter partitioning ratio of petiole in Soyang river of Chunchon was the highest of all others but it was lowest of all others in ground water of Suwon. Rhizome weight of main stem in Chunchon was showed possitive correlation with plant height and fresh top weight and in Suwon it was showed possitive correlation with root weight and high possitive correlation with No. of total leaves and No. of tillers.

• Korean Journal of Medicinal Crop Science
• /
• v.13 no.3
• /
• pp.77-80
• /
• 2005

This study was conducted to determine effects of cutting date, cutting position, and node number on rooting of Rubus buergeri Miquel. Apical, medial, and basal parts of the vines emerged in 2002 were cut on June 26, August 2, and September 4, in 2002. Vine pieces with one to four nodes taken from the medial parts of the vines were also cut on July 10. The greatest rooting (69.6%) occurred in cuttings taken on August 2. Cutting taken on June 26 had the longest roots (23.1 cm). Rooting and new shoot growth markedly decreased at September 4 cutting. The greatest rooting (55.4%) occurred in cuttings taken from medial part of the vines and followed by apical parts (45.6%), and basal parts (25.4%). The other traits were not significantly affected by cutting position. Rooting percentage and percent new shoot growth were not significantly affected by node number, but root number and root dry weight per cutting, and new shoot growth increased with node number.