• IMMUNE NETWORK
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• 제1권1호
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• pp.7-13
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• 2001

Currently 18 monoclonal antibodies were approved by FDA for inj ection into humans for therapeutic or diagnostic purpose. And 146 clinical trials are under way to evaluate the efficacy of monoclonal antibodies as anti-cancer agents, which comprise 9 % of clinical trials in cancer therapy field. When considering a lot of disappointment and worries existed in this field during the past 15 years, this boom could be called as resurrection. Antibodies have several merits over small molecule drug. First of all it is easier and faster in development, as proper immunization of the target proteins usually raises good antibody response. The side effects of antibodies are more likely to be checked out in immunohistomchemical staining of whole human tissues. Antibody has better pharmacokinetics, which means a longer half-life. And it is non-toxic as it is purely a "natural drug. Vast array of methods was developed to get the recombinant antibodies to be used as drug. The mice with human immunoglobulin genes were generated. Fully human antibodies can be developed in fast and easy way from these mice through immunization. These mice could make even human monoclonal antibodies against any human antigen like albumin. The concept of combinatorial library was also actively adopted for this purpose. Specific antibodies can be screened out from phage, mRNA, ribosomal library displaying recombinant antibodies like single chain Fvs or Fabs. Then the coding genes of these specific antibodies are obtained from the selected protein-gene units, and used for industrial scale production. Both $na\ddot{i}ve$ and immunized libraries are proved to be effective for this purpose. In post-map arena, antibodies are receiving another spotlight as molecular probes against numerous targets screened out from functional genomics or proteomics. Actually many of these antibodies used for this purpose are already human ones. Through alliance of these two actively growing research areas, antibody would play a central role in target discovery and drug development.

• Journal of Ecology and Environment
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• 제42권2호
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• pp.85-89
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• 2018

Observations and video documentation of interactions between animals living in dens, cavities, and other enclosed spaces are difficult, but they play an important role in field biology, ecology, and conservation. For example, bird parents visiting nests and feeding their nestlings may provide crucial information for testing of ecological hypotheses and may easily attract attention of participants of citizen-science ecological and conservation projects. Because of the nest concealment of cavity-nesting birds, their behaviors in the nest can only be studied by using video surveillance. Professional wildlife surveillance systems are extremely expensive. Here, we describe an inexpensive video setup that can be constructed with relatively little effort and is more affordable than any previously described system. We anticipate that the relatively low cost of about 250 USD for a battery-operated system is an important feature for citizen-science type of projects and for applications in heavily populated areas where the potential for theft and vandalism may be high. Based on our experiences, we provide methodological advice on practical aspects of using this system in the field for ecological research on birds. We highlight the low cost, easiness of construction, and potential availability to a large number of observers taking part in wildlife monitoring projects, and we offer technical help to participants of such research projects.

• The Korean Journal of Physiology and Pharmacology
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• 제8권2호
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• pp.77-81
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• 2004

The loss of neurons and synaptic contacts following cerebral ischemia may lead to a synaptic plastic modification, which may contribute to the functional recovery after a brain lesion. Using synapsin I and GAP-43 as markers, we investigated the neuronal cell death and the synaptic plastic modification in the rat hippocampus of a middle cerebral artery occlusion (MCAO) model. Cresyl violet staining revealed that neuronal cell damage occurred after 2 h of MCAO, which progressed during reperfusion for 2 weeks. The immunoreactivity of synapsin I and GAP-43 was increased in the stratum lucidum in the CA3 subfield as well as in the inner and outer molecular layers of dentate gyrus in the hippocampus at reperfusion for 2 weeks. The immunoreactivity of phosphosynapsin was increased in the stratum lucidum in the CA3 subfield during reperfusion for 1 week. Our data suggest that the increase in the synapsin I and GAP-43 immunoreactivity probably mediates either the functional adaptation of the neurons through reactive synaptogenesis from the pre-existing presynaptic nerve terminals or the structural remodeling of their axonal connections in the areas with ischemic loss of target cells. Furthermore, phosphosynapsin may play some role in the synaptic plastic adaptations before or during reactive synaptogenesis after the MCAO.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.78.2-79
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• 2003

Pepper defensin ( CADEFl) clone was isolated from cDNA library constructed from pepper leaves infected with avirulent strain Bv5-4a of Xanthomonu campestris pv. vesicatoria. The deduced amino acid sequence of CADEFl is 82-64％ identical to that of other plant defensins. Putative protein encoded by CADEFl gene consists of 78 amino acids and 8 conserved cysteine residues to form four structure-stabilizing disulfide bridges. Transcription of the CADEF1 gene was earlier and stronger induced by X campestris pv. vesicatoria infection in the incompatible than in the compatible interaction. CADEF1 mRNA was constitutively expressed in stem, root and green fruit of pepper. Transcripts of CADEFl gene drastically accumulated in pepper leaf tissues treated With Salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), hydrogen Peroxide (H$_2$O$_2$), benzothiadiazole (BTH) and DL-${\beta}$-amino-n-butyric acid (BABA). In situ hybridization results revealed that CADEF1 mRNA was localized in the phloem areas of vascular bundles in leaf tissues treated with exogenous SA, MeJA and ABA. Strong accumulation of CADEF1 mRNA occurred in pepper leaves in response to wounding, high salinity and drought stress. These results suggest that bacterial pathogen infection, abiotic elicitors and some environmental stresses may play a significant role in signal transduction pathway for CADEF1 gene expression.

• 한국의류학회지
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• 제31권5호
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• pp.765-776
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• 2007

Customer expectations and requirements for products play an important role in product planning for companies and decision making process for the consumer. These expectations are expressed by product qualities that consumers consider important when they purchase. Therefore, to identify quality elements that reflect consumer requirements would be a useful guide for companies. The purposes of this study are to find out quality factors of apparel product, to identify apparel product quality elements using Kano's theory, to find attributes of product which improvement are required. Women over 20 years-old from metropolitan areas in South Korea participated in the study and a quota sampling method was used. A questionnaire was arranged with four separate subject sections, importance of quality, Kano's questionnaires, and demographics. Data from 525 questionnaires were used for the statistical analysis. The results were as follows: Six dimensions of product quality(i.e., usefulness, performance, aesthetic, symbol, individuality and appearance) were identified. According to Kano's quality elements, performance was categorized into must-be quality which could lead to product dissatisfaction. Usefulness and appearance were categorized into one-dimensional quality which lead to both satisfaction and dissatisfaction. Aesthetic, symbol, and individuality was categorized into attractive quality which could lead to satisfaction. Findings of this study provide both industry and academic researchers with a guide to increase customer satisfaction in the product development process.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.133-138
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• 2005

Automotive pulleys play a key role in driving the cooling pump, oil pump, air-conditioner and so on by using an engine power. Researches on design processes and technologies of the pulleys can be found in many literatures. On the other hand, the areas related to manufacturing processes of the pulleys have been treated negligently. Vast data extracted from various information systems are transformed, integrated, and summarized to become a special database for helping users make a decision. The database, namely the data warehouse has been popularly used in the marketing and customer management of enterprises and recently applied to improve the design and manufacturing processes. In this study the manufacturing process of pulleys were analyzed through the intensive investigation of shop-floors and the interviews with workers and managers. The defects generated during a manufacturing process were categorized in a few types and the causes of defects examined for extracting the dominant parameters in the setup process for producing pulleys. As the first step to construct the data warehouse for the manufacturing processes of pulleys, authors proposed its architecture focused on the reduction of defect rate during the setup process.

• 대한공업교육학회지
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• 제45권2호
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• pp.86-107
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• 2020

이 연구의 목적은 발명교육을 운영하는 다양한 교육기관에서 발명교육 프로그램을 설계하고 준비할 때 활용할 수 있는 기초적 평가준거를 제시하여 발명교육의 효과와 효율성을 높이기 위함이다. 이에 따른 연구의 결과는 다음과 같다. 첫째, 발명교육 프로그램의 단계는 '계획(Planning), 준비(Preparation), 과정(Process), 산출(Product)'의 4단계로 '4P 단계'로 명명하였다. 둘째, 발명교육 프로그램의 계획 단계 평가 영역은 '목표 설정, 요구 분석', 교육과정 설계'이다. 준비 단계 평가 영역은 '발명교육 프로그램 개발, 인적 자원 확보, 물적 자원 확보'이다. 과정 단계 평가 영역은 '프로그램 운영, 프로그램 관리, 학습자 관리'이다. 산출 단계 평가 영역은 '교육 실적, 성취 결과, 교육 평가'이다. 이상의 발명교육 프로그램 평가준거는 발명교육 프로그램의 질을 제고하고 관리할 수 있는 역할을 할 것으로 판단된다. 또한, 다양한 발명교육 기관에서의 환류와 관계 전문가들의 타당화 과정을 통한 조정과정을 거쳐 최종적으로 발명교육 프로그램의 평가준거를 개발하는데 기초 자료가 될 것으로 판단된다.

• 정보보호학회논문지
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• 제23권4호
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• pp.767-779
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• 2013

개인정보보호 분야는 타 분야에 비해 통계 및 현황에 대한 정보가 부족하다. 또한 개인정보보호 분야는 정보화, 정보보안, 법 등 어느 분야에도 일방적으로 속하지 않아 부수적으로만 실태 파악이 되고 있어 앞으로도 한계가 있을 것이다. 본 논문에서는 약 11만 개의 개인정보처리자가 공개하고 있는 개인정보 처리방침을 이용하여 방대한 양의 데이터를 수집하고 저장하여 이를 분석할 수 있는 방안을 제시하고 실제 결과를 보여준다. 제시된 방안은 데이터의 품질이 보장되고 시간과 비용을 절감할 수 있어 기존 방식에 비해 결과에 대한 정확성과 현황 파악에 대한 신속성에 있어 유리하다. 또한 다양한 분석이 가능하여 개인정보보호에 대한 실태 파악의 새로운 돌파구 역할을 할 수 있다. 본 논문에서는 개인정보보호와 빅데이터 분야의 접목을 통해 개인정보보호의 발전 방안을 제시하고 있다.

• 한국조경학회지
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• 제34권6호
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• pp.39-53
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• 2007

Unlike conventional roof landscaping, where various kinds of plants and structures are employed, a grass roof is a roof on which herbaceous plants are grown in planting medium and which is not accessed or maintained, mainly because it doesn't have sufficient load capacity to support a regular roof garden. They are mostly built on existing roofs, whether flat slab or gabled. Planting on roofs has numerous advantages, such as creating a biotope, purifying urban air, adding moisture to the atmosphere, storing rain water, preventing flash floods, reducing energy use for heating and air conditioning, enhancing the urban landscape and providing relaxation to the city dwellers, not to mention the alleviation of global warming by absorbing $CO_2$. In addition to the general merits of roof planting, the grass roof has its own unique qualities. Only herbaceous species are planted on the roof, resulting in light weight which allows roofs of existing buildings to be planted without structural reinforcement. The species chosen are mostly short, tough perennials that don't need to be maintained. These conditions provide an ideal situation where massive planting can be done in urban areas where roofs are often the only and definitely the largest space available to be planted. If roofs are planted on a massive scale they can play a significant role in alleviating global warming, heat island effects and energy shortages. Despite the advantages of grass roofs, there are some problems. The most significant problem is the invasion of neighboring plants. They may be brought in with the planting medium, by birds or by wind. These plants have little aesthetic value comparing to the chosen species and are usually taller. Eventually they dominate and prevail over the original species. The intended planting design disappears and the roof comes to look wild. Since the primary value of a grass roof is ecological, a change in attitude towards what constitutes beauty on the roofscape is necessary. Instead of keeping the roof neat through constant maintenance, people must learn that the wild grass with bird's nests on their roof is more beautiful as it is.

• 농촌계획
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• 제20권1호
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• pp.13-26
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• 2014

The purpose of this study is to identify characteristics of the landscape changes in Gwangjyu city and Icheon city, the suburban areas on the outskirts of the Seoul metropolitan area, by focusing on the causes of the urban sprawl and the resultant morphological characteristics of landscape degradation. Particular attention has been given to the 'advantages for developing suburb including geographical proximity and land price', 'institutional regulation such as land-use regulation' and 'community solidarity' as drivers for possible introduction of external factors. The types of landscape change were divided into three, after having on-the-ground research on eleven chosen towns and interviews with head of a village: Overwhelming Change, Separated and Small-Scale Change. It was found that, in general, the quality of landscape was best for the small-scale change type, followed by separated and overwhelming change types. While the types of landscape change are correlated with the geographical proximity, its relevance with land regulation is relatively weak. The study found that imposing a land-use regulation as a means to prevent changes in suburban village landscape may not be fully effective. Also it analyzed that villages could better manage with keen interest the surrounding landscape if there is a high degree of solidarity within the village community. Therefore, the sense of community could play an important complementary role to land-use regulation which does not suffice in itself to manage suburban landscape.