• 제목/요약/키워드: rol

검색결과 48건 처리시간 0.022초

Achromobacter Protease I (API)의 기질특이성 결정기의 동정과 변이체[D225E]의 Ornithine 기질에 대한 촉매활성 (Identification of Substrate Specificity Determinant of Achromobacter Protease I (API) and Catalytic Activity of Mutant D225E for Ornithine Substrate)

  • 임성일;권오진;최청
    • Applied Biological Chemistry
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    • 제40권3호
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    • pp.189-195
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    • 1997
  • 부위특이적 변이제작을 통하여 Achromobacter protease I (API)의 lysin에 대한특이성의 구조적 근거를 조사하였다. Lysine 특이성을 결정하는 결정기로서 예상되는 잔기중의 하나인 Glu190을 glutamine, aspartic acid, leucine으로 치환하였을 경우, 전구체단백질은 Iysine 특이성을 가진 완전한 활성효소로서 발현되어 자기촉매적으로 processing되었다. 다른 하나의 잔기인 Asp225을 asparagine, leucine으로 치환한 변이체에서는 불활성 전구체인 pro-API으로서 발현되었으나, 변이체 (D225E)의 경우는 서서히 자기촉매적으로 maturation 되었다. 성숙체인 변이체 (D225E)의 Iysylendopeptidase 활성은 wild-type APl의 0.25%이였으며, 이 활성의 감소는 기질 Iysine과 효소와의 친화력 감소가 주 원인이었다. 이러한 결과로부터 Asp225가 Iysylendopeptidase의 엄격한 기질특이성에 결정적인 역할을 담당한다는 것이 시사되었다. 한편, 변이체 (D225E)는 ornithine 기질에 대해서 효소활성 이 검출되지 않았다. 이 변이체의 촉매잔기인 Ser194가 native API과 마찬가지로 DFP, PMSF와 근본적으로 동일한 반응성이 있는 것으로 나타나 225번 위치의 methylene 1 unit 더 긴 측사는 subsite P1의 methylene 1 unit 짧은 측사에 의해 보상되지 않는다는 것을 알 수 있었다.

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상권방문 추진동기와 몰입, 만족, 재방문 의도 (Visit Push Motivation for a Trading Area and Flow, Satisfaction, and Revisit Intention)

  • 이수덕;이용기
    • 유통과학연구
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    • 제16권9호
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    • pp.65-77
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    • 2018
  • Purpose - A trading area is very closely related to consumer life. A trading area is a cultural and social space that consumes culture and promotes human relationships as well as an economic space where consumers live their daily lives. In this context, a trading area research should be conducted objectively and empirically because it deals with the activities of consumer's life. The purpose of this study is to identify the intrinsic psychological motivation(push motivation) caused when consumers visit a trading area and to demonstrate how the push motivation for a trading area influence on consumer's flow, satisfaction, revisit intention. Research design, data, and methodology - In order to develop research hypotheses for this study, the development procedures for push motivation scale are as follows; (1) generating initial pool of items based on previous studies, (2) expert judgement to evaluate content and face validity, and (3) assessing convergent and discriminant validity using confirmatory factor analysis. In order to achieve these purposes, online surveys were conducted on frequent or familiar visitors to the trading areas around the Gangnam, Kunkuk University and Hongik University Station. Among the 1,343 questionnaires collected, 1,157 cases were analyzed by using SPSS 22.0 and SmartPLS 3.0 statistical package program, except for 186 responses in which responses were judged to be unfaithful. Results - The push motivation was classified into five sub-dimensions of excitement/stimulus, rest/relaxation, exit/refreshing, knowledge/learning and human relationship promotion as multidimensional and complex factors composed of individual and social-related dimensions. The excitement/stimulus and human relationship promotion of push motivation have positive effects on satisfaction. However, all dimensions of the push motivation have positive effects on flow. And flow has a positive effect on satisfaction and revisit intention. Meanwhile, the mediation test using boostrapping shows that flow plays a full mediating role in the relationship between rest/relaxation, exit/refreshing, knowledge/learning and satisfaction, but a partial mediating rol e between excitement/stimulus, human relationship promotion and satisfaction. Finally, satisfaction plays a partial mediating role between flow and revisit intention. Conclusions - This study shows that the push motivation is multidimensional and compositive depending on the situation of a consumer. In addition, it is found that the human relationship promotion(a social-related motivation) has a much more important effect on flow and satisfaction than other push motivations of individual dimensions. It also shows that satisfaction increases when consumers are being flowed at their visit and degree of revisit intention also grows as satisfaction increases. As implications of this study, a marketer should try to understand consumer's visit motivation at first and then develop factors that increase their flow, satisfaction, revisit intention. It also requires a marketer to approach subjects on a trading area more objectively and empirically based on the psychology and behavior of consumers, in order to establish a proper and efficient strategy on development of a trading area.

인삼 모상근 유도를 위한 최적 조건 (The Optimum Conditions for Induction of Ginseng Hairy Roots)

  • 양덕춘;김용해;양덕조;신성련;최광태
    • 한국자원식물학회지
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    • 제12권1호
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    • pp.1-9
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    • 1999
  • 인삼 뿌리 절편으로 부터 모상근 유기를 위한 최적 조건을 확립하고자 Agrobacterium rhirogenes와 인삼 뿌리 절편의 항생제 내성 조사 및 최적의 모상근 유도 배지를 조사하기 위하여 수행하였다. NaOCl로 인삼 뿌리를 멸균하였을 때, 오염 정도가 감소하면서 조직의 손상이 일어나지 않는 NaOCl의 농도는 7% NaOCl에서 15-20분, 9% NaOCl에서 5분으로 나타났다. 인삼근은 년수가 증가할수록 오염 정도가 심하였으며, 특히 6년근중 표피가 있는 처리구는 오염 정도가 매우 높았다. Agrobacterium의 성장억제를 위한 항생제는 tetracycline이 가장 효과적이었으며, 30mg/L 이상의 농도에서 균의 성장이 억제되었다. 하지만 30mg/L tetracycline에서 인삼 조직이 고사하였으며, cefotaxime(500mg/L), carbenicillin(500mg/L)에서 균의 성장을 완전히 억제하였으며, 조직의 손상이 일어나지 않았다. 3년근 인삼에서 모상근 유도을 위한 배지로는 1/2MS 배지에 500mg/L의 cefotaxime이 첨가된 배지가 가장 좋았으며, 인삼 뿌리 절편에 Agrobacterium을 발라주는 것 보다는 균과 공동배양할때가 절편이 좋았다. Agrobacterium접종 2주 후부터 callus가 유기되기 시작한 후, 다시 2주 후에 모상근이 유도되었다. 유도된 hairy roots는 PCR에 의하여 rol C유전자를 조사함으로서 형질전환체임을 확인하였다.

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한국산 고감염 Agrobacterium spp의 분리 및 연초의 형질전환에 이용 (Isolation of Hypervirulent Agrobacterium spp from Korea and Application for Transformation of Tobacco)

  • 양덕춘;정재훈;이정명
    • 식물조직배양학회지
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    • 제25권3호
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    • pp.207-217
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    • 1998
  • 한국에서 자생한 자연산 tumor 조직과 근권토양으로부터 형질전환율이 높은 hypervirulent Agrobacterium spp를 분리하기 위해서 Salix, Diospyros, Populus 및 Malus에서 형성된 tumor 조직과 근권토양을 채취하괴 Schroth 선택배지와 New and Kerr 배지를 이용하여 78 균주의 colony를 특성에 따라 분리하였다. 이중에서 48 균주가 당근 disc에서 tumor를 형성하였으며 tumor를 형성한 균주중 형성시기가 빠르며 크기가 커 hypervirulent 균주로 생각되는 A. tumefaciens SP101을 biotype 1, 그리고 A. tumefaciens SM042를 biotype 2로 동정하였다. 토양중에서 선발한 A. tumefaciens SM042와 disarmed A. tumefaciens PC2760에 kanamycin 저항성 유전자를 함유하고 있는 binary vector pGA643을 도입하여 conjugant인 A. tumefaciens SM643과 A. tumefaciens PC643을 kanamycin과 tetracycline이 함유된 최소배지에서 획득하였다. 연초의 형질전환을 위해서 conjugant Agrobacterium과 연초조직을 동시배양 후 2,4-D와 kanamycin이 함유된 선발배지에 치상하여 형질전환을 유도한 결과 A. tumefaciens SM643이 A. tumefaciens PC643보다 더 많은 캘러스가 형성되었다. 그러나 A. tumefaciens PC643을 사용한 형질전환 캘러스는 대부분 friable한 캘러스로 유도되었으며 정상적으로 식물체로 생장하였으나 A. tumefaciens SM643을 사용한 캘러스는 매우 딱딱하며 둥그런 형태의 캘러스와 friable한 캘러스가 혼재한 상태로 생장하였으며, 이중에서 friable한 캘러스는 정상적인 shoot가 형성되었으나 딱딱한 캘러스로 유도된 형질전환체는 식물체로 형성되지 않고 반구형 미색의 전형적인 tumor 캘러스로 생장하였다. 한편 형질전환시 캘러스를 유도하지 않고 직접 shoot를 형성시킨 결과 disarmed Ti-plasmid를 사용하지 않고 wild-type Ti-plasmid를 사용해도 정상적인 형질전환체를 획득할 수 있었다.

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119구급대원의 심폐소생술 성적 분석 - 병원전 심정지를 중심으로 - (Factors Affecting the Survivals of Out-of-hospital Cardiac Arrests by 119 Fire Service)

  • 강병우
    • 한국응급구조학회지
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    • 제9권2호
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    • pp.111-128
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    • 2005
  • Background: Cardiac arrest is one of the most critical diseases which can likely lead to severe cerebral disability or brain death when the cases can not recover their circulation within 10 minutes. Saving out-of-hospital cardiac arrest cases is a recent concern in Korea. Resuscitation has become an important multidisciplinary branch of medicine, demanding a spectrum of skills and attracting a plethora of specialities and organizations. The best survival can be achieved if all the following links have been optimized : rapid access, and early CPR, defibrillation and ACLS, Since the "Utstein Style" was advocated in 1991, many reports about out-of-hospital cardiac arrest have been published based on this guideline. These differences prevent valid inter-hospital and international comparisons. However, it is not known how effective resuscitation has become to the patients. In other words, there are no guidelines for reviewing, reporting, and conducting research on resuscitation in Korea. This dissertation aims to provide the basic data for a unified reporting guideline of resuscitation in Korea and evaluating the out-of-hospital factors associated with survival discharge of out-of-hospital cardiac arrest. Methods: As for this study, uses the collected data about Out-of-hospital cardiac arrests at 4 area, from January, 2005 to April. 2005. With a retrospective study, 174 cases were analyzed. The data was recorded based on the Out-of-Hospital Utstein Style. Results: Resuscitation was performed on 174 out-of-hospital cardiac arrest cases at the 4 area 14 patients(8.1%) recovered their spontaneous circulation. Overall, the ROSC of the out-of-hospital cardiac arrest patients was 8.1%, which was poorer than that of western countries. Gender distribution was 50 females(28.7%) and 124 males(71.3%), approximately twice as many males as females. ROSC of witnessed arrests was found out to be 97.7%. The ratio of the witnessed arrest groups showed higher results than that of unwitnessed arrest groups in the above-examined cases. Cardiac etiology consisted of cardiac(33.5%), non-cardiac(45.7%), trauma(20.1%), and unknown(6.0%). Cardiac was the best performance. Initial rhythm showed Ventricular Tachycardia/pulseless Ventricular Fibrillation in 8 patients(6.0%), asystole in 100(75.2%) and unknown in 25(18.8%). The results of the Ventricular Tachycardia/pulseless Ventricular Fibrillation showed higher results than the others cases, The proportion of the cardiogenic cause was 33.5%, which was only half of western countries. Ventricular Tachycardia/pulseless Ventricular Fibrillation is relatively rare. These differences were due to the prevalent pattern of Out-of-hospital cardiac arrest as well as prematurity of the EMSS. Bystander CPR was practiced on 13 patients(7.52%). ROSC was shown in 46.2% cases. CPR by EMT was carried out on 167 cases(96.5%). ACLS by EMf was rare. From collapse, 4 cases(2.6%) arrived to ED within 6 minutes. 13 (8.6%) within 10 minutes, and 49(32.5%) over 31 minutes. The sooner the patients arrived, the greater the ratio of ROSC and discharged alive became, and the same with collapse time to ROSC. As the results of the logistic regression analysis, ROSC was found out to be highly influenced by the time of ED arrival from collapse and Ventricular Tachycardia/pulseless Ventricular Fibrillation. Therefore, the ratio of ROSC depends on not any single factor but various intervention factors. Conclusion: This dissertation presents the following suggestions and directions of the study hereafter. First, the first step for a chain of survival should be taken to activate EMSS early with a phone as soon as cardiac arrests are witnessed. Second, it is keenly needed that emergency medical technicians should be increased through emergency education for living. Third, it is necessary to establish the emergency transportation system. Fourth, most of the Koreans have little understanding of EMT and the present operation systems have many problems, which should be fundamentally changed. Fifth, it is required to have an active medical control over Out-of-hospital CPR, And proper psychological supports should be given not only to patients themselves and their family but also individuals who are engaged in emergency situation. Finally, through studies hereafter on nationwide, comprehensive, and standard forms, it is needed to examine into the biological figures of human body, causes and trends of cardiac arrests, and then, to enhance the survival rate of Out-of-hospital cardiac arrests. Korean guidelines for Cardiopulmonary resuscitation need to be made.

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동력경운기를 중심으로한 맥류재배의 기계화 -관작업체계 확립에 관한 연구 (Study on the Establishment of Barley Cultivation System using the Power Tiller)

  • 이영렬;최규홍
    • Journal of Biosystems Engineering
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    • 제3권2호
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    • pp.22-33
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    • 1978
  • To maintain the self-sufficiency of food production , it is required to improved the productivity and increase the effective utilization of land in the double cropping areas using improved cultivation technology. The following reasons encourage this cours of action because the overlapping rice harvest and barely seeding seasons complete for labour, the lower price of barley makes it financially less attractive to grow and these two facts together are responsible for a decrease in barley being planted in certain more seasonably marginal double cropping areas. Fro these reasons, it is desirable to carry out tests to improve the current cultivation techniques . In this interest , the following studies were carried out in the experimental field located at Banweol-myeon, Whasung-jkun, Gyeonggi-do from October 1977 to July 1978 in order to establish the mechanical method of barley cultivation using the power tiller. The summarized results are as follows. 1. The work performance in the seeding operation using the rotary barley seeder was 68 minutes per 10 ares which compares favourably with 408 min/10a using the conventional method. This is only one sixth of the time required by th conventional method. The operating costs using the rotary barley seeder was 1, 463 won per 10 areas as against 3, 486 Won per 10 ares for the conventional method, showing a saving of about 50% in comparison with the conventional method. 2. In the manure spreading operation, the work performance was 25 min/10a for the manure spreading machine , compared to 1089 min/10a for the manual operations. This is about one fourth of the time required by the conventional method, The operating costs were 810 Won/10a for the machine and 857 Won/10a for the manual labour. This cost shows little advantage by the machine over the manual labor costs. The conventional method of manure spreading will continue pending decision which will develope and improve the machine.3. Work performance in the rolling operation using the barley rolIer was 30 min/lOa which compares favourably with 135 min/10a using conventional method. This was one fourth of the time required by the conventional method. The operating costs were 514 Won/103. for the machine and 1, 003 Won/lOa for manual labour. In the weed control operation, the work performance and operating costs were 45min/lOa, 1, 399won/10a for the herbcide application using the power sprayer, 1, 149min/10a 8, 541won/lOa for the conventional method respectively. This is 26 times higher efficiency in comparison to the conventional method. 4. In the harvesting operation using the reaper binder and tiller attachment, the work performance was 60min/lOa and represents a cost of 2, 039won/10a. The con\ulcornerventional method took 640min/10a at a cost of 4, 757won/lOa. The reaper binder showns a saving of one tenth over the conventional method. The automatic thresher is already recommended for the current situations, and is now being used. 5. From a comparison of the results of the above trials, the serial cultivation system using the attachment for the power tiller such as the rotary barley seeder is now to be recommended for the current barley cultivation system. It is also recommend from these results that the mechanized technology now available must be used to improve and maintain the increase in barley production. It is seen th1t this is the only course now avaihble to solve the peak seasonal requirements of labour needed for l11rvesting and seeding between rice and barley cultivation.

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동력경운기의 경사지견인 및 주행특성에 관한 연구 (III)-동력경운의 경사지 견인성능- (Study on the Travel and Tractive Characteristics of the Two-Wheel Tractor on the General Slope Land(III)-Tractive Performance of Power Tiller-)

  • 송현갑;정창주
    • Journal of Biosystems Engineering
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    • 제3권2호
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    • pp.35-61
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    • 1978
  • 동력경운깅가 일반 경사지에서 견인주행하는 경우 견인주행성능과 주행특성을 구명하기 위하여 토양의 종류 및 상태는 일정하게 하고 지면의 기하학적 조건과 견인주행속도 및 견인하중을 변수로 하여 외부동력전달계의 시점인 좌우차륜과 토양간에 발생하는 차륜구동력 및 굴름정항과 Engine에서 구동륜까지 내부전달계를 통하여 전달된 동력의 이론치와 실험치와의 부합여부를 검정하고 부가적으로 동력경운기가 경사지기계화의 동력기로써의 가능성여부와 문제점을 찾으려한다.

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동력경운기의 경사지견인 및 주행특성에 관한 연구 (Ⅲ)-동력경운의 경사지 견인성능- (Study on the Travel and Tractive Characteristics of the Two-Wheel Tractor on the General Slope Land(Ⅲ)-Tractive Performance of Power Tiller-)

  • 송현갑;정창주
    • Journal of Biosystems Engineering
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    • 제3권2호
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    • pp.34-34
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    • 1978
  • 동력경운깅가 일반 경사지에서 견인주행하는 경우 견인주행성능과 주행특성을 구명하기 위하여 토양의 종류 및 상태는 일정하게 하고 지면의 기하학적 조건과 견인주행속도 및 견인하중을 변수로 하여 외부동력전달계의 시점인 좌우차륜과 토양간에 발생하는 차륜구동력 및 굴름정항과 Engine에서 구동륜까지 내부전달계를 통하여 전달된 동력의 이론치와 실험치와의 부합여부를 검정하고 부가적으로 동력경운기가 경사지기계화의 동력기로써의 가능성여부와 문제점을 찾으려한다.