• Korean Journal of Mathematics
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• v.23 no.3
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• pp.447-456
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• 2015

In this paper we show that some operators defined on the Banach space with an unconditional basis and $L^1({\mu})$ into a Banach space with the RNP have liftable operators.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2008.06a
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• pp.117-118
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• 2008

• 대한생식의학회:학술대회논문집
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• 2001.11a
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• pp.116.2-116.2
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• 2001

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.714-718
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• 2014

Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possible roles for caspase-6 have been proposed, the identities and functions of proteins that interact with caspase-6 remain uncertain. In this study, we established a cell line expressing tandem affinity purification (TAP)-tagged caspase- 6 and then used LC-MS/MS proteomic analysis to analyze the caspase-6 interactome. Eight candidate caspase-6-interacting proteins were identified. Of these, five proteins (hnRNP-M, DHX38, ASPP2, MTA2, and UACA) were subsequently examined by co-immunoprecipitation for interactions with caspase-6. Thus, we identified two novel members of the caspase-6 interactome: hnRNP-M and MTA2.

• The Journal of Internal Korean Medicine
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• v.32 no.1
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• pp.33-42
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• 2011

Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.427-436
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• 2017

A fixed-precision-level sampling plan was developed to establish control of the two-spotted spider mite, Tetranychus urticae, in two strawberry greenhouses (conventional plot, natural enemy plot). T. urticae was sampled by taking a three-leaflet leaf (1 stalk) from each plant (3 three-leaflet leaves) from each sampling position. Each leaflet was divided into three different units (1-leaflet, 2-leaflet, and 3-leaflet units) to compare relative net precision (RNP) values for selection of the appropriate sampling unit. The relative net precision values indicated that a 1-leaflet unit was more precise and cost-efficient than other units. The spatial distribution analysis was performed using Taylor's power law (TPL). Homogeneity of the TPL parameters in each greenhouse was evaluated by using the analysis of covariance (ANCOVA). A fixed-precision-level sequential sampling plan was developed using the parameters of TPL generated from the combined data of the conventional plot and natural enemy plot in a 1-leaflet sampling unit. Sequential classification sampling plans were also developed using the action threshold of 3 and 10 mites for pooled data. Using the results obtained in the independent data, simulated validation of the developed sampling plan by Resampling validation for sampling plan (RVSP) indicated a reasonable level of precision.

• Bulletin of the Korean Mathematical Society
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• v.33 no.2
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• pp.277-287
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• 1996

The theory of integration of functions with values in a Banach space has long been a fruitful area of study. In the eight years from 1933 to 1940, seminal papers in this area were written by Bochner, Gelfand, Pettis, Birhoff and Phillips. Out of this flourish of activity, two integrals have proved to be of lasting: the Bochner integral of strongly measurable function. Through the forty years since 1940, the Bochner integral has a thriving prosperous history. But unfortunately nearly forty years had passed until 1976 without a significant improvement after B. J. Pettis's original paper in 1938 [cf. 11].

• Animal cells and systems
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• v.8 no.3
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• pp.213-220
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• 2004

MicroRNA and siRNA (small interfering RNA), representative members of small RNA, exert their effects on target gene expression through association with protein complexes called miRNP (microRNA associated ribonucleoproteins) and RISC (RNA induced silencing complex), respectively. Although the protein complexes are yet to be fully characterized, human EIF2C2 protein has been identified as a component of both miRNP and RISC. In this report, we raised antiserum against EIF2C2 in order to begin understanding the protein complexes. An immunoblot result indicates that EIF2C2 protein is ubiquitously expressed in a variety of cell lines from human and mouse. EIF2C2 protein exists in both cellular compartments, as indicated by an immunoblot assay with a nuclear extract and a cytosolic fraction (S100 fraction) from HeLa S3 lysate. Depletion of EIF2C1 or EIF2C2 protein resulted in a decrease of microRNA, suggesting a possible role of these proteins in microRNA stability or biogenesis. We also prepared antiserum against dsRNA binding protein PACT, whose homologs in C. elegans and Drosophila are known to have a role in the RNAi (RNA interference) pathway. The expression of PACT protein was also observed in a wide range of cell lines.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.198.1-198
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• 2000

No Abstract, See Full Text

• Korean Journal of Microbiology
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• v.46 no.4
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• pp.401-404
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• 2010

Tho1 is a RNA-binding protein that assembles co-transcriptionally onto the nascent mRNA and is thought to be involved in mRNP biogenesis and mature mRNA export to cytoplasm in budding yeast. In fission yeast Schizosaccharomyces pombe, a homologue of THO1 (spTho1) was identified based on sequence alignment. A deletion mutant in a diploid strain was constructed by replacing one of spTho1-coding region with an ura4+ gene using one-step gene disruption method. Tetrad analysis showed that the spTho1 was not essential for growth. The spTho1 mutant did not show any defects of bulk mRNA export. However, over-expression of spTho1 from strong nmt1 promoter caused the growth defects and accumulation of poly(A)$^+$ RNA in the nucleus. These results suggest that spTho1 is involved in mRNA export from the nucleus to cytoplasm though it is not essential.