• Title/Summary/Keyword: risk process

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Porphyromonas Gingivalis Invasion of Human Aortic Smooth Muscle Cells

  • Lee, Seoung-Man;Lee, Hyeon-Woo;Lee, Jin-Yong
    • International Journal of Oral Biology
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    • v.33 no.4
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    • pp.163-177
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    • 2008
  • Periodontal disease, a form of chronic inflammatory bacterial infectious disease, is known to be a risk factor for cardiovascular disease (CVD). Porphyromonas gingivalis has been implicated in periodontal disease and widely studied for its role in the pathogenesis of CVD. A previous study demonstrating that periodontopathic P. gingivalis is involved in CVD showed that invasion of endothelial cells by the bacterium is accompanied by an increase in cytokine production, which may result in vascular atherosclerotic changes. The present study was performed in order to further elucidate the role of P. gingivalis in the process of atherosclerosis and CVD. For this purpose, invasion of human aortic smooth muscle cells (HASMC) by P. gingivalis 381 and its isogenic mutants of KDP150 ($fimA^-$), CW120 ($ppk^-$) and KS7 ($relA^-$) was assessed using a metronidazole protection assay. Wild type P. gingivalis invaded HASMCs with an efficiency of 0.12%. In contrast, KDP150 failed to demonstrate any invasive ability. CW120 and KS7 showed relatively higher invasion efficiencies, but results for these variants were still negligible when compared to the wild type invasiveness. These results suggest that fimbriae are required for invasion and that energy metabolism in association with regulatory genes involved in stress and stringent response may also be important for this process. ELISA assays revealed that the invasive P. gingivalis 381 increased production of the proinflammatory cytokine interleukin (IL)-$1{\beta}$ and the chemotactic cytokines (chemokine) IL (interleukin)-8 and monocyte chemotactic (MCP) protein-1 during the 30-90 min incubation periods (P<0.05). Expression of RANTES (regulation upon activation, normal T cell expressed and secreted) and Toll-like receptor (TLR)-4, a pattern recognition receptor (PRR), was increased in HASMCs infected with P. gingivalis 381 by RT-PCR analysis. P. gingivalis infection did not alter interferon-$\gamma$-inducible protein-10 expression in HASMCs. HASMC nonspecific necrosis and apoptotic cell death were measured by lactate dehydrogenase (LDH) and caspase activity assays, respectively. LDH release from HASMCs and HAMC caspase activity were significantly higher after a 90 min incubation with P. gingivalis 381. Taken together, P. gingivalis invasion of HASMCs induces inflammatory cytokine production, apoptotic cell death, and expression of TLR-4, a PRR which may react with the bacterial molecules and induce the expression of the chemokines IL-8, MCP-1 and RANTES. Overall, these results suggest that invasive P. gingivalis may participate in the pathogenesis of atherosclerosis, leading to CVD.

Eco-Friendly Production Process of N-Hydroxysuccinimide from Succinic Anhydride (Succinic Anhydride로부터 N-Hydroxysuccinimide의 친환경 생산공정 개발)

  • Goo, Bon Suk;Baek, Jeong yeon;Park, Hwa In;Jung, In Chan;Kim, Woo-Sun
    • Clean Technology
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    • v.26 no.2
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    • pp.91-95
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    • 2020
  • A new eco-friendly synthetic method for N-hydroxysuccinimide (NHS), widely used in the pharmaceutical and fine chemical industries, is developed. Conventional synthesis method yields NHS of about 70% after its reaction with NH2OH to succinic acid. In this method, NHS can be obtained using low-cost succinic acid, but a great deal of solvents are required as an extraction method to purify NHS, while the work-up process is complicated, resulting in low yield. In addition, there is a safety risk due to the high reaction temperature for commercial production, and it is not economical due to the high cost of production from the generation of much waste because of an acid catalyst and the use of various solvents. In order to make up for this shortcoming, this study used succinic anhydride as a raw material under low temperature reaction and developed a new eco-friendly industrial synthesis method using isobutyl alcohol for a single solvent and non-catalytic reaction. The economic evaluation confirms that there is a cost reduction effect of about 20%. In the future, based on this result, studies may establish a commercial production technology through scale-up research and proceed with foreign technology transfer.

Development and Application of Green Infrastructure Planning Framework for Improving Urban Water Cycle: Focused on Yeonje-Gu and Nam-Gu in Busan, Korea (도시물순환 개선을 위한 그린인프라 계획 프레임워크 개발 및 시범적용 - 부산시 연제구 및 남구를 대상으로 -)

  • Kang, JungEun;Lee, MoungJin;Koo, YouSeong;Cho, YeonHee
    • Journal of Environmental Policy
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    • v.13 no.3
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    • pp.43-73
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    • 2014
  • Cities in Korea have rapidly urbanized and they are not well prepared for natural disasters which have been increased by climate change. In particular, they often struggle with urban flooding. Recently, green infrastructure has been emphasized as a critical strategy for flood mitigation in developed countries due to its capability to infiltrate water into the ground, provide the ability to absorb and store rainfall, and contribute to mitigating floods. However, in Korea, green infrastructure planning only focuses on esthetic functions or accessibility, and does not think how other functions such as flood mitigation, can be effectively realized. Based on this, we address this critical gap by suggesting the new green infrastructure planning framework for improving urban water cycle and maximizing flood mitigation capacity. This framework includes flood vulnerability assessment for identifying flood risk area and deciding suitable locations for green infrastructure. We propose the use of the combination of frequency ratio model and GIS for flood vulnerability assessment. The framework also includes the selection process of green infrastructure practices under local conditions such as geography, flood experience and finance. Finally, we applied this planning framework to the case study area, namely YeonJe-gu an Nam-gu in Busan. We expect this framework will be incorporated into green infrastructure spatial planning to provide effective decision making process regarding location and design of green infrastructure.

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Renaissance of Geographic Education in the United States since 1980: Its Dynamic Process and Implications to Geographic Education in Korea (1980년대 이후 美國 地理敎育 復興運動의 展開過程과 그 示唆點: 地理學, 地理敎育, 그리고 敎育政策의 關係)

  • Seo, Tae-Yeol
    • Journal of the Korean Geographical Society
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    • v.28 no.2
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    • pp.163-178
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    • 1993
  • The purpose of this paper is to provide a better understanding of the unprecedented reform movement of geographic education in the United States since 1980 and extract some implications from this movement for geographic education in Korea. For the purpose, the history to this movement was reviewed through following three stages. In the first stage(1980~1984: form :HSGP" to :"Guideline"), the voluntary improvement movement appeared at California and the orgni-zational movement began in 1982 such as the Committee on Geography and International Knowledge. The national educational refrom imperatives, presented at "A Nation at Risk", and "Back to Basics" movement provided good opportunities to resurrect geography as a basic subject. For next real resurrection movement, the very important document "Guidelines for Geographic Education" was published at 1984. In the second stage(1985~1989: from "Guide-lines" to "Public"), the "Guideline" gave power-full motives and foci for reconstructiong the contents of geography, especially by the five fundamental themes(Location, Place, Relation-ships within Places, Movement, and Region). Also GENIP as the symbol of unity of all four major geography organization(AAG, NCGE, NGS, AGS) contributed to expanding and stren-gthening geography education. Also Geography Educagtion Program of NGS was a smart and well organized program to improve geographic education through it's a five strategies: Grass-roots organization(Alliances), Teacher education, Pu-blic awareness, Educational materials develo-pment, Targeted outreach to education decision-makers. In the late 1980s, the last focus of movement was the Public awareness and Edua-ction decision-making. In the third stage(1990-present: from "Public" to "Core Subject"), the initiative pendulum swung from geography organization to nation curricu-lum. In this National Curriculum, Geography was approved as a "Core Subject" and The 1994 National Geography Assessment Framework was constructed to assess the outcome of student's education in geography in grades, 4,8, and 12. Some Implications extracted from the process and contents of renaissance movement of geogr-aphic education in the Uinted States since 1980 are as follows. First, It shows the importance of the unity and target assignment among the geography organization. Second, interactive relationship between the academic geography and school geography develops each other. Third, teacher education, including pre-service education, including pre-service education and in-service education, is a key element to improve the quality of geography. And teacher organization is a good clearing house to exchange information for good geography. Forth, the positive and active response to changes in socketies such as globalism and inter-nationalizing, national education policy, and the trend of pedagogy is needed to rejuvenate geo-graphic education. Above all, we need to establish a well organized and powerfull program, sophisticated activities strategies, and long-term implementa-tion plan if we want more and better school geography.

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Development of a Decision Making Model for Construction Management in LNG Plant Construction - Focused on Construction Stage - (LNG 공사의 건설사업관리 의사결정지원모델 개발 - 시공단계 중심 -)

  • Park, Hwan Pyo;Han, Jae Goo;Chin, Kyung Ho
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.3
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    • pp.47-57
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    • 2014
  • LNG plant projects tend to be implemented in overseas owing to its characteristics, so their project management scheme is somewhat different from those of general projects. Value chain in a LNG plant project includes exploration/production of gases, physical liquefaction/chemical conversion processes, transportation and storage. Key factors in the chain include liquefaction process (including ultra-low temperature liquefaction) to convert natural gas into liquid materials or fuel, and Front End Engineering Design (FEED) package, as well as Engineering, Procurement and Construction (EPC) technology comprising control, operation and construction. Success of a complex LNG plant project implemented in overseas depends on decision-making process in project management. Accordingly, to develop a decision-making model in of plant construction, the study extracted none factors in project management by EPC stage and assessed importance of each factor. The result showed that items in both project management and project risk management are important. Especially, the study developed a decision-making model in the construction stage of a LNG plant project based on the project management factors and importance assessment. The developed decision-making model would lay groundwork in building a decision-making system in construction stage of project management.

An Analysis of Concentrate Competency in Bidding Process for Overseas Project of Domestic Construction Companies (국내건설기업의 해외 프로젝트 입찰단계 우선집중역량 분석)

  • Kim, Ji Hyung;Kim, Yea Sang
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.3
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    • pp.23-30
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    • 2014
  • Overseas construction orders of domestic companies had been increased significantly in a short period from $10.9 billion in 2005 to $64.9 billion in 2012. However experts are concerned that quality of growth will not support quantitative growth. This study suggest concentrate competencies for not only winning in bid but also successful execution of domestic companies. 4 Steps which are Pre-Marketing, Bid-Preparation, Bidding, Contract Phase are defined for competency assessment. After drawing specific tasks by phase, survey asking importance and capability of the tasks to the person in charge of bidding and site was conducted by utilizing Likert 7 point scale. According to the result of IPA analysis, domestic construction companies need to ensure internal competencies; Project risk review, Bid team organization, and external competencies; Client needs grasp, Localization strategy, Cooperation establishment. Hearafter domestic construction companies will be able to utilize the result of the study for setting bidding strategy for winning in bid and improving earning rate.

Development of Safety Management Information System for Gas Industries Using Database (데이터베이스를 이용한 가스산업시설의 안전관리정보시스템 구축)

  • Um Sung-In;Kim Sung-Bin;Kim Yun-Hwa;Baek Jong-Bae;Kim In-Won;Ko Jae-Wook
    • Journal of the Korean Institute of Gas
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    • v.2 no.2
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    • pp.48-54
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    • 1998
  • In this study a computerized prototype system was developed with Safety Management Information System(SMIS version 1.0) as a main system and database as subsystems to handle information. Safety management information consists of management aspects and technical elements, but SMIS consists of 4 modules of technical elements to interrelate safety technologies closely. SMIS enables gas industries to manage process safety information effectively and to evaluate hazards. The results from SMIS can be used to the operation manual and the emergency plan. Data base consists of 3 modules of accident data, material data, and equipment data to support SMIS. Also, the case study results proved the usefulness of SMIS for searching and accumulating process safety data. Especially, MIS which has the database suggests a formal structure for scattered raw safety data in gas industries and brings reduction of man power and time.

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Methodology of Prior Art Search Based on Hierarchical Citation Analysis (계층적 인용관계분석을 통한 선행기술 탐색방법론)

  • Kang, Jiho;Kim, Jongchan;Lee, Joonhyuck;Park, Sangsung;Jang, Dongsik
    • Journal of the Korean Institute of Intelligent Systems
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    • v.27 no.1
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    • pp.72-78
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    • 2017
  • Prior art search is a core process of technology management performed by inventors and applicants, patent examiners, and employees in the patent industry. As a result of insufficient academic research on a systematic prior art search methodology, the process has been often carried out depending on the subjective judgment of researchers. Previous studies on exploring prior arts based on semantics have also have the risk of underestimating the similarity of major prior arts due to the nature of patent documents where the same technical ideas are expressed in various terms. In this study, we propose an effective prior art search methodology based on hierarchical citation analysis, which provides a clear criterion for selecting core prior arts by calculating weights according to the relative importance of the collected patents. In order to verify the feasibility of the proposed methodology, a case study was conducted to explore the core prior art of one patent in the display field. As a result, 10 core prior art candidates were selected out of the 206 precedent patents.

Bio-technology and Citizen Participation - A Case Study of Re-combinant DNA Debate (생명공학과 시민참여 - 재조합 DNA 논쟁에 대한 사례 연구)

  • Kim Dong-Kwang
    • Journal of Science and Technology Studies
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    • v.2 no.1 s.3
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    • pp.107-134
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    • 2002
  • Recently, society's relationship with science is in a critical phase. There was never a time when the issues involving science were more exciting. Many people are deeply uneasy about the safety and ethics of bio-technology. On the other hand, this could be interpreted as a dynamic process in which a new relationship between science and public is reshaping. And in this new relationship citizen participation in science is not a option but a necessity. This article is a case study of 1970's re-combinant DNA debate. Early 1970's bio-technology saw the emergence of an enormously powerful new methodology, recombinant DNA technology. But, at the outset, this technology posed many potential dangers. Concern over possible hazards prompted hot debates and conflict between leading scientists and community, In this process Cambridge Laboratory Experiment Lion Review Board (CERB) is organized by Cambridge City Coun il for assessing the possible risk of recombinant DAN technique. This is one of the first citizen-initiated participation movement in bio-technology. And the debates has come to be a principal focus for many of the most important questions concerning citizen participation in science. This study make a attempt to analyze CERB case. In conclusion, we can confirm the possibility of civil participation in science policy making and decision making from CERB case study. Still, we also realize the limit of CERB case.

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Analysis of COVID-19 Pandemic in terms of War Theory (전쟁이론 관점에서의 COVID-19 Pandemic 분석)

  • Han, Seung Jo;We, Jinwoo
    • Convergence Security Journal
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    • v.21 no.3
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    • pp.81-91
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    • 2021
  • The purpose of this study is to examine COVID-19 situation in temrs of war-theory and to find out ways to overcome it. Just as the war changes the paradigm in the international situation and the national crisis management system, the current COVID-19 pandemic is bringing about the entry of the so-called "New Normal" era having the characteristics including untact culture. Although academic research on COVID-19 is mainly dealt with in terms of medical, tourism, and economics, the military research has not yet begun from the perspective of military science or war theory. In the concept of a comprehensive crisis that COVID-19 can cause enormous damage to the life and property of a country, it can be regarded as a target or enemy to be overcome. Among various war theories, the similarities with COVID-19 incident are analyzed in terms of the nature and aspect of the war and the factors of victory. Qualitative and questionnaire analysis results show that the COVID-19 outbreak is very similar to war when considering a variety of war-characteristics. In addition this research proposes ways to overcome COVID-19 based on the victorious factors of the past war, and predicts the impact of the international community after the end of COVID-19 pandemic. As a result of analyzing the priority of overcoming factors through the Analytical Hierarchy Process (AHP) shows that clear goals and establishment of alliances should be prioritized for successfully overcoming COVID-19.