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Development of Seed Culture Using Soybean for Mass Production of Lovastatin with Aspergillus terreus ATCC 20542 Mutant (대두를 이용한 Lovastatin 대량생산용 Seed Culture의 제조기술)

  • Kim, Soo-Jung;Ko, Hee-Sun;Kim, Hyun-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.5
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    • pp.666-670
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    • 2008
  • Lovastatin (Mevinolin, Monacolin K) is a well-known drug for the therapy of hypercholesterolemia. It is an important fungal secondary metabolite as it inhibits 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) which catalyzes a major rate-limiting step in the biosynthesis of cholesterol. Both soybeans and black soybeans with Aspergillus terreus ATCC 20542 mutant were used as the seed culture for the mass production of lovastatin. The production of lovastatin in soybean seed culture of Asp. terreus was twofold compared to that of black soybean seed culture. The effect of two different vessels (petri dish and Erlenmeyer flask) on lovastatin production was also studied. The production of lovastatin on petri dish was tenfold to that of Erlenmeyer flask. Furthermore, the most lovastatin production on rice bran was achieved when the soybean seed culture was treated by heat shock at $30^{\circ}C$ for 1 hour, representing 82% of HMG-CoA reductase inhibition in the koji extract. We estimated that the heat treated soybean seed culture could be a new method for the mass production of lovastatin.

Manufacture and Physiological Functionality of Korean Traditional Alcoholic Beverage by Using Lily (Lilium lancifolium) Scales (참나리(Lilium lancifolium) 구근을 이용한 민속발효주의 제조 및 생리활성)

  • Lee, Ka-Soon;Kim, Gwan-Hou;Kim, Hyun-Ho;Lee, Chan-Gu;Lee, Ji-Yong;Lee, Hee-Duck;Oh, Man-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.5
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    • pp.598-604
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    • 2008
  • This study was carried out to develop a new traditional alcoholic beverage by using lily scale in order to utilize lily scale as a new matter for nutritious foods. The condition of alcohol fermentation was investigated by the addition of 2.5, 5.0, 7.5, 10.0, 12.5 and 15.0% dried lily scale and 10, 20, 30, 40 and 50% raw lily scale into mash, respectively. The maximum amount of ethanol was produced when 15% dried lily scale and 20% raw lily scale were added to cooked rice at $25^{\circ}C$ for 10 days. In overall acceptability of sensory evaluation, the LSD-5 alcoholic beverage (added 5% dried lily scale into mash) and LSR-20 alcoholic beverage (added 20% raw lily scale into mash) showed the best acceptability. Physiological functionalities of LSD-5 and LSR-20 alcoholic beverage were investigated. Electron donating ability by DPPH solution ($16.5{\sim}23.2%$), SOD-like activity ($20.4{\sim}24.7%$), ACE inhibitory activity ($24.7%{\sim}78.2%$) and tyrosinase inhibitory activity ($57.4{\sim}62.6%$) of LSD-5 and LSR-20 alcoholic beverage were better than the control (non-added liquor). Moreover, acceptability and physiological functionalities of LSD-5 alcoholic beverage are better than LSR-20.

Importance-Satisfaction and Usage of Traditional Market Foods of Undergraduate Students in Jeonbuk Area (전북 일부 지역 대학생들의 전통시장 음식 이용 현황 및 중요도-만족도 분석)

  • Lee, In-Seon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.1
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    • pp.123-131
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    • 2017
  • The aim of this paper was to provide basic data on revitalizing traditional markets in 389 undergraduate students who had purchased foods sold at a traditional market in order to understand the current state of usage of traditional market foods and to analyze degree of importance and satisfaction. From September 2015 to November 2015, questionnaires were filled out by respondents. Of the 389 questionnaires that were returned, 343 were used while 46 were discarded as incomplete. In the traditional market, high popularity was detected in foods such as 'Hotteok', 'stir-fried rice cake', 'Korean sausage', and so on in that order, which were mainly simple foods. There was a tendency that 'taste', 'hygiene', 'price', and 'quality' were considered as important, all of which scored more than 4 points for all residence types. In the difference analysis of the degree of satisfaction and importance in traditional market foods according to residence types, degree of importance was much higher than that of satisfaction for all selective attributes. In the results of ISA analysis for attributes of traditional market foods, those selling food in the second quadrant need to make more effort to improve 'hygiene' and 'quality' of food.

An Analysis on The Consumption Pattern of Korean Farm Households (1960~1968) -The Influence of Income Level on The Consumption Pattern- (농가 가계의 소비구조 분석 (1960-1968) -소득수준이 소비구조에 미치고 영향분석을 중심으로-)

  • 최은숙
    • Journal of the Korean Home Economics Association
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    • v.8 no.1
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    • pp.181-197
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    • 1970
  • Farm households consumption pattern from 1960 to 1968 was analized as follows. First, the influence of the price on the consumption pattern was observed by year for 9 years. Second, the influence of farm households and by size of the consmption pattern was analized by year for whole household and by size of the arable land. Third, the present status and problems of farm families consumption pattern was attempted to be found out compared with the pattern of all families of Seoul. Forth, the pattern of food expenditure which has the largest proportion in the living expenditure was analized. Following results and conclusions are obtained from the above analysis.1. The average nominal increase rate of all farm households has been increased gradually for 9 years, mostly due to the rise in household goods prices paid by farmers. 2. The living expenditure varies with the price and the disposal income, The influence of the latter is greater than that of the former. 3. The Engel's Coefficient of farmers. the average of which was 54.9%, recently tends to decrease gradually. The larger is the size of arable land, the lower is the Coefficient of farm households. But the Coefficient of farm households is higher than that of city families. 4. In general the proportion of food expenditure has a great influence on the consumption pattern, The average percentage of miscellaneous expenditure is 23.9% and it is next to food expenditure. The relationship between them is going reversely. Housing expenditure has usually the constant proportion. But the difference of the proportion between farm households (4.0%) and city families (17.1%) is considerable. Clothing expenditure and fuel and light expenditure have small variations. The former tends to increase with the income, and the latter tends to decrease with it or is constant. 5. Considering the food expenditure pattern, the average percentage (78.4%)of staple food of farm household is remarkably high compared with Seoul (48.3%) and other cities (54.0%). The decrease of the percentage of staple food expenditure in farm households is not so much as cities. 6. The propertion of the staple food expenditure of Farm families don't have so much differences by the size of arable land. But the rice proportion of staple foods has the tendency to increase with the income level. Subsidery food expenditure doesn't increase by year and by size of the land, while the consumption of meat and manufactured foods tends to increase with size of the land. But even big farm households don't reach to the level of cities in consumption of them. 7. Food consumption pattern may be influenced by the factors such as the knowledge of wife about nutrition, customs, consumption habits, and so on. The difference between farm households and city families in food consumption pattern is inferred from the above factors. Presently, the increasing income of the people promote the consumption of all items of living expenditure both in cities and farm households. But the Engel's Coeffcient and the proportion of the staple food expenditure is expected to decrease in farm households more than in cities.

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A Comparison of Characteristics between Danish and Korean Farmhouses -In Reference to Farmhouse Plans, Use of Rooms including Furniture Arrangement, and Building Materials-

  • Choi, Jung-Shin
    • International Journal of Human Ecology
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    • v.8 no.1
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    • pp.67-78
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    • 2007
  • The aim of this paper was to compare differences and similarities of farmhouses between Denmark and Korea as a cross-cultural study. Farmhouses built during the 18th and 19th centuries in Denmark, and ones from Chosun Dynasty (AD. 1392-1910) in Korea were targets of this study. Literature study and field trips to districts and open-air museums in both countries were used. Field trips were carried out during summer of 2005 in Denmark, and fall of 2005 in Korea. Detailed comparisons focused on farmhouse plans, the use of rooms and furnishing, and building materials. As a conclusion, some differences were found between the two countries. In terms of farmhouse plans, square types with four wings, and parallel types were the most frequent forms in Denmark, while a few types, such as the "I", "L", "U" shapes and square types, were more frequently found in Korea. In Denmark, the most important room was the dwelling-room, in which daily family life took place. Every member of the family slept, ate, and worked here, and kept geese and young animals during the winter season. Therefore, this room was laid out in the center of the house. However, Anbang, the most important room in Korea, was not situated in the best part of the house. Instead, the Daechung-maru occupied the center and the best place because the daechung-maru, in which the ancestral ceremony was held, was thought of as more important than a place for live people. Also the use of rooms and furnishing was quite different between the two countries, reflecting each life style. Danish furnishings represented practical daily life, while Korean furnishings reflected more conceptual aspects. It might have resulted from Confucianism in the Chosun Dynasty, which ruled daily life and even influenced use of rooms and furnishings. In other word, philosophy influenced common peoples' daily life and living environment. With reference to building materials, there was rarely a difference between the two countries. Major materials for farmhouse were quite common, such as half-timbering wood and clay, but the difference was in bricks and roof coverings. Bricks were rarely used in Korean farmhouses, while rice-straw was rare for the roof of a Danish farmhouse. Of course, the choice of materials was strongly affected by the local surroundings and overall environment. This result can give some clues for design ideas when architects and interior designers plan housing that is adapted for local peoples. This kind of cross-cultural study can also contribute to one's understanding of foreign culture, especially in housing and culture. And, it can broaden one's insight of design ideas for designers who have to compete frequently with designers in foreign countries.

Stability of the Pigments from Monascus purpuresu CBS 281.34 (Monascus purpureus CBS 281.34가 생성하는 홍국 색소의 안정성)

  • 임성일;곽은정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.711-715
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    • 2004
  • The stability of 80% ethanol-soluble pigments from Monasus purpureus CBS 281.34 was investigated according to storage temperature, pH and addition of organic acid. Also, the stability of ethanol-soluble pigment in aqueous system was examined after the addition of distilled water in the range of 0∼80% during the storage at 1$0^{\circ}C$ and 2$0^{\circ}C$ for 4 weeks with water soluble pigment. The heat stability was the highest (9.74%) when the 80% ethanol-soluble pigments were stored at 1$0^{\circ}C$ for 4 weeks. However, the 80% ethanol-soluble pigments stored at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 24 h and 12 h greatly decreased by 23.06% and 30.36%, respectively. Although the 80% ethanol-soluble pigments were stable in the range of pH 4∼8, the degradation rate of pigment increased at pH 2 and PH 10.80% ethanol extract was adjusted to PH 4 by adding organic acids. The rate of pigment degradation was not different from control for 4 weeks. Red pigment was stable in the treatment of organic acids. And the stability of ethanol-soluble pigment in aqueous system was gradually decreased as the pigment content and storage time increased. Additionally, the stability of ethanol-soluble pigment was higher at 1$0^{\circ}C$ than at 2$0^{\circ}C$.

Screening of Monascus Strains for Antimicrobial Activity and Effect of Change of Nutrients and Incubation Conditions on Antimicrobial Activity (항균성 Monascus 균주의 Screening 및 영양원과 배양조건이 항균활성에 미치는 영향)

  • 마재형;황한준
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.6
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    • pp.1080-1086
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    • 1996
  • Monascus strains were isolated from Ang-Khak for the screening of antimicrobial activity. Two Monascus isolates, No.116 and No.481, were selected because they showed strong antimicrobial activity. Effect of various nutrients and incubation conditions on antimicrobial activity were different between two isolates. Strong antimicrobial activity of isolate No.116 was observed in the medium with 8% sucrose and $0.8%(NH_4)_2SO_4,$ 0.5% $KH_2PO_4and$ 0.5% $MgSO_4,$ while isolate No.481 required 8% sucrose, 1.6~2% $(NH_4)_2SO_4,$ 0.5% $MgSO_4and$ 0.5% $FeSO_4for$ the highest activity. The strong antimicroial activity was observed when both isolates were incubated on rice extract broth with initial pH of 5.3. The optiimum incubation temperature for the highest antimicrobial activity was $32.5^{\circ}C.$ With optimal conditions for the highest antimicrobial activity, isolate No.116 and No. 481 were both active for 51 hours or longer against test organisms Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecium.

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Antimicrobial Activity of ε-Polylysine Mixtures against Food-borne Pathogens (ε-Polylysine 혼합제제들의 식품부패균에 대한 항균효과)

  • 고은미;김병용
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.705-710
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    • 2004
  • This study was conducted to investigate the difference of antimicrobial activity between $\varepsilon$-polylysine/grapefruit seed extract mixture and $\varepsilon$-polylysine/glyceride mixture against food-borne pathogens. The minimum inhibitory concentration (MIC) of the $\varepsilon$-polylysine/grapefruit seed extract mixture was 100 times lower than that of the $\varepsilon$-polylysine/glyceride mixture. In case of the $\varepsilon$-polylysine/glyceride mixture the MIC of Bacillus cereus (0.1 $\mu$L/mL) and Pseudomonas aeruginosa (0.1 $\mu$L/mL) were lower than that of Escherishia coli (15 $\mu$L/mL). When rices were cooked with the $\varepsilon$-polylysine/glyceride mixture the number of total microbial cell was decreased during storage at 2$0^{\circ}C$ as concentration of the $\varepsilon$-polylysine/glyceride mixture increased. The antimicrobial activity was the highest against Escherishia coli as the concentration of the $\varepsilon$-polylysine/glyceride mixture increased. Sensory terms such as taste, flavor and texture were not significantly different in cooked rices prepared with 0.5% $\varepsilon$-polylysine/glyceride mixture, but there was significantly different in cooked rices prepared with 1% (p<0.05) in the overall acceptability, indicating that the cooked rice with 0.5% $\varepsilon$-polylysine/glyceride mixture was recommended.

Demand for Elderly Food Development: Relation to Oral and Overall Health -Focused on the Elderly Who Are Using Senior Welfare Centers in Seoul- (구강 및 전신 건강 기반 고령자용 식품 개발을 위한 요구도 조사 -서울 일부 지역 복지관 이용 노인을 대상으로-)

  • Lee, Geum-Yong;Han, Jung-Ah
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.3
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    • pp.370-378
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    • 2015
  • Oral health is a critical factor for diagnosing overall health, as poor oral health has a negative impact on food intake and results in deterioration of nutritional status. The purpose of this study was to research the needs for food developments for the elderly based on their oral and overall health status. Oral status was significantly associated with self-reported health status, and dry mouth (lack of saliva) especially showed a significant relationship with negative self-perception of both oral and overall health. For both elderly men and women, fish and leafy vegetables were the most preferred side dishes. Bread, rice cake, and beverage-type foods were also preferred for snack development. Disease status of the elderly was significantly related with their preference for food development. For instance, those with diabetes were more likely to ask for anti-diabetic diets. The most important factor for the elderly in choosing food was nutritional balance, and less than 3,000 won was cited as an affordable price. The results could be utilized as basic data to develop foods for the elderly.

On the Mushroom Cultivation of Oak (Quercus) Chip and Used Culture Medium of Lentinus into Feedstuff (참나무류(類) 칩을 이용(利用)한 표고버섯재배(栽培)와 폐잔사(廢殘渣) 사료화(飼料化))

  • Min, Du Sik
    • Journal of Korean Society of Forest Science
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    • v.80 no.4
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    • pp.436-444
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    • 1991
  • To increase both the production of mushrooms and the usages of the residues (used media) for roughage, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The contents of crude proteins(1.82-4.55%) in the used media were higher than those in woods (c.a. 0.7%), and the total digestible nutrients (44.0-46.0%) in the used medium were not much different from those in rice straws (c.a.48.0%). 4. The contents of some essential amino acids (methionine in the used medium of the tannic acid added PC or S.D. and isoleucine, phenylalanine and lysine in the used medium of the untreated PC or SD) were higher than those in the control (raw media). The contents of a few other essential amino acids (threonine, valine and arginine) were lower in the used media. Most nonessential amino acids in the used media, particularly in the used bed-log, appeared to decrease than those in the control.

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