• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.3
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• pp.214-219
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• 1991

To clarify the relationship between ambient water temperature and heading characteristics of rice (Oryza sativa L.), twelve of varieties rice were grown in a cold tolerance screening field where water temperature was controlled by continuous cold water irrigation system to test the cold tolerance of rice. When cold water was continuously irrigated with 5 cm of water depth, the water had stagnated for about three hours, and the water temperature increased gradually from inlet toward outlet in the experimental plot. The fluctuation of water temperature was well synchronized with that of ambient air temperature, and the water temperature near outlet became higher than the air temperature at the vegetative phase, while became lower at the reproductive phase of rice plant community. The leaf development rates on main culm increased by increased water temperature. The rice varities, Fukuhikari, Sangpungbyeo and YR3486-16-2 were more sensitive than the others in the response of leaf development to water temperature. However, Janack and Milyang 42 were comparatively less sensitive to water temperature in leaf development. Janack and Paro -white rices required longer days to develop one leaf on main culm at reproductive phase than at vegetative phase. Varietal difference in days required to develop one leaf on main culm of rice plant was more distinctive at the reproductive phase when water temperature was relatively lower than at the vegetative phase with relatively higher water temperature condition. No difference was found between the growth phases, vegetative and reproductive, in the response of average leaf developmental rates to water temperature under the similar air temperature condition. The estimated average days required to develop one leaf on main culm decreased by 1.3 day by 1$^{\circ}C$ increase in water temperature. Varietal differences in the total number of leaves on main culm depended upon the water temperature, in which the varieties such as Fukuhikari, Gwangmyeon-gbyeo, China 988, and YR3486-16-2 showed increased one leaf by increased water temperature, while Sobaekbyeo, Paro-white, Sangpungbyeo, Pungsanbyeo, Samgangbyeo, and Milyang 42 were kept at the same leaf number regardless of water temperature. However, the total leaf number on main culm and days to heading of Janack increased by increased water temperature. The other varieties showed the shortened days to heading by the increase in water temperature with noticeable varietal differences regardless of the variation in the total number of leaves on main culm.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.275-280
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• 2011

BACKGROUND: The present study was conducted to find out the suitable method for organic rice production on the basis of different seedling raising methods at nine eco-friendly agricultural units of Samgi, Mangsung, Iksan and Sungsan, Gusan of Jeollabuk-do, during 2009-10. METHODS AND RESULTS: On the basis of yield and physiological parameters, pot seeding method was found to be superior to drill seeding and broadcast seeding methods. The number of panicle, grain, the precent of ripened grains, and the 1,000 grain weight, were better in pot seeding method. Maximum yield and other attributes were recorded in rice, cultivated with seedlings raised by pot and broadcast seeding method. Number of panicle/hill and grain/panicle was 10.4% and 35.1% higher than the broadcast seeding method, respectively. Yield also showed 8.8% increase in pot seeding method as compare to broadcast seeding method. Higher grain yield was obtained when 56 hills/$3.3m^2$ of rice seedlings were used as compare to 50 hills/$3.3m^2$ raised by pot seeding method and 70 hills/$3.3m^2$ of broadcast seeding method. Lodging was minimum in seedlings raised with pot seeding method as thickness of third internode was more (9.0%) than the seedlings, raised with broadcast seeding method. Root length and dry weight also showed similar tendency i.e. 13.8% and 25.3% higher, respectively. CONCLUSION(s): Quality and grade of rice, cultivated with pot seeding method was better than broadcast seeding method. Head rice was 4.4% higher; and protein content and broken rice grown by pot seeding method were 0.4% and 1.8% lower than broadcast seeding method, respectively.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.413-421
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• 2011

The main objective of this study was to develop the multi-resistance lines to insects(brown planthopper; BPH, rice green leafhopper; GRH) and disease(blast; BL, bacterial blight; BB and rice stripe virus disease;RSV) with good grain quality and plant type by combining conventional breeding and marker assisted selection(MAS) and to eliminate the linkage drag effects between Bph1 gene and culm length, we conducted MAS of Bph1 gene in advanced backcross and double cross progenies. 'Nampyeong', 'Junam' and 'Milyang220' were used as the parent in this study. 'Milyang220' was used as the donor of brown planthopper resistance gene Bph1 with tall culm length. Two backcross progenies were developed using two recipients 'Nampyeong' carrying GRH resistance gene Grh3(t) with good grain appearance and 'Junam' harboring bacterial blight resistance gene Xa3 with short culm length. Two $BC_1$ generations were resulted from the backcrossing of the $F_1$ plants with recurrent parents 'Nampyeong' and 'Junam'. The second rounds of backcrossing($BC_2$) were derived from the cross of selected resistant $BC_1F_1$ plants based on heterozygous genotype of RM28493 linked to Bph1 gene. The double crossed population was constructed from the cross of between each heterozygous $BC_2F_1$ plants at RM28493 locus of '$Nampyeong^*3$ / Milyang220' and '$Junam^*3$ / Milyang220'., The homozygous alleles in Bph1 gene were selected using co-dominant DNA marker RM28493 in double crossed population. Eighty-five lines with multi-resistance to BL, BB, RSV, GRH and BPH were selected by bio-assay and MAS in generation of double crossing. The culm length, head rice ratio and yield of the selected multi resistance lines was ranged from 71 to 88 cm, from 51 to 93%, from 449 to 629 kg/10a. respectively. We can select a promising multi resistance line similar with 'Nampyeong' of major agronomic traits such as culm legnth, head rice ratio and yield. It was designated as Milyang265. Finally this study was developed the multi resistant varieties against to insects and diseases with the good grain quality 'Milyang265' by the advanced backcross and double cross combining MAS and it can be used as genetic resources of multi-resistance to insect and diseases in rice breeding programs.

• Korean Journal of Soil Science and Fertilizer
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• v.5 no.1
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• pp.33-39
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• 1972

For the increase of productivity, canopy structure and nutritional status in various productivity grades (high>700, medium>600 and low>500kg/l0a as head rice) were investigated in a study using Jinheung(Oryza sativa L.) for the increase of producticity with following results: 1. High yield plot showed greater leaf area and slow decrease of it after heading stage. While low yield plot had rapidly decrease of leaf area. 2. High yield plot showed higher light transmission ratio (LTR) durnig early grow stage, low LTR. during late grow stage, and higher leaf area per LTR. 3. Net assimilation ratio was decrease with the increase of leaf area index (LAI) and it was always higher in high yield plot. 4. High yield plant had greater amount of total nitrogen uptake, slow uptake rate, and considerable uptake after heading while low yield plant showed higher uptake of nitrogen in early stage of growth and no uptake after heading. 5. The percent ratio starch at heading to total at harvesting was 40% for low yield plant and 10% for highyield plant inderting that high yield depends much on past-heading starch synthesis. 6. Main factor determining secondary yield was ripened grain ratio.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.11
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• pp.1653-1659
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• 2015

This study compared physicochemical properties and antioxidant activities according to different harvest times in five varieties of black rice (BR). Five cultivars of BR, Nunkeunheukchal (NKHC), Sinnongheugchal (SNHC), Sintoheugmi (STHM), Josaengheugchal (JSHC), and Heugjinju (HJJ), were selected for the study. Cultivars were transplanted on June 20th and cultivated by the Department of Southern Area Crop Science, NICS in Miryang, Korea. Heading dates of NKHC, SNHC, STHM, JSHC, and HJJ were Aug. 17th, Aug. 30th, Sep. 1st, Aug. 20th, and Aug. 12th, respectively. The five different harvest times were as follows; 35, 40, 45, 50, and 55 days after heading date. The highest level of anthocyanin was 35 days after heading date in all varieties. Levels of anthocyanin in NKHC, SNHC, STHM, JSHC, and HJJ were 164, 203, 251, 67, and 210 mg/100 g, respectively. Content of anthocyanin significantly decreased 35 days after heading in four varieties, excluding JSHC. Highest DPPH $IC_{50}$ values were 57, 29, 10, and $27{\mu}g/mL$ in NKHC, SN HC, STHM, and JSHC extracts, respectively. Highest ABTS $IC_{50}$ values were 24, 13, 9, and $11{\mu}g/mL$ in NKHC, SNHC, STHM, and JSHC extracts, respectively. The antioxidant activities were highest 35 days after the heading date and significantly decreased in four varieties, excluding HJJ. The antioxidant activity of HJJ showed no significant difference, according to harvest time. This study suggests that the content of anthocyanin and antioxidant activity could be considered as factors affecting optimal harvest period to produce highly qualified black rice.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1785-1790
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• 2014

This study was carried out to compare the physicochemical characteristics and sensory qualities of Sikhye (Korean traditional rice beverage) prepared with foxtail millet, proso millet, and sorghum. The cultivated varieties were Setaria italica Beauv. cv. Samdamae (SDM) and Samdachal (SDC), Panicum miliaceum L. cv. Ibaekchal (IBC), Sorghum bicolor (L.) Moench cv. Nampungchal (NPC), Donganme (DAM), Oryza sativa cv. Ilpum (IP), and Baegokchal (BOC). The brix degrees of SDM, SDC, IBC, NPC, DAM, IP, and BOC Sikhye were 9.53, 8.63, 5.67, 7.57, 6.27, 12.50, and $12.27^{\circ}Bx$, respectively. There were no significant differences in pH (5.99~6.10) among the groups. The highest turbidity was 1.07 in DAM Sikhye. The L-value, a-value, and b-value were 30.85~41.11, -0.34~2.52, and 2.56~5.67, respectively. Total polyphenol contents of SDM, SDC, IBC, NPC, DAM, IP, and BOC Sikhye were 241.52, 213.69, 202.34, 258.25, 193.24, 160.81, and $170.31{\mu}g\;GAE/mL$, respectively. Total flavonoid contents of Sikhye were $19.21{\sim}54.30{\mu}g\;CE/mL$. The highest DPPH and ABTS radical scavenging activities were $16.85{\pm}0.39$ and $64.75{\pm}2.92mg\;TE/100mL$ in DAM Sikhye, respectively. Finally, the sensory evaluation results indicate that there were significant differences in appearance, aroma, and taste between the groups, and SDM Sikhye was similar with IP and BOC.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.4
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• pp.465-474
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• 1996

The study was carried out to determine the proper concentration of plant growth regulator, kinetin on alleviating copper toxicity for two rice cultivars of seed germination. The results were as followings : Soaking treatment of kinetin 10$^{-3}$ M increased the germination rate of both cultivars, Ilpumbyeo and Hyangmibyeo 1 by 92% and 88% as compared with copper treatment (60ppm). But the soaking treatment effect of plant growth regulator, kinetin was not recognized at the kinetin 10$^{-4}$ M and 10$^{-5}$ M. Chlorophyll content of both rices was higher than that of Hyangmibyeo 1. Copper content of Ilpumbyeo was higher in leaf than in seed part. At the 3 days after treatment of copper 60ppm, both cultivars of treatment of kinetin 1O-3M showed the somewhat thin bands at the 35 and 40kDa compared with others. A new protein band pattern was only appeared to kinetin 1O-3M at approximately 54.4kDa(M. W) at the 7 days after treatment of copper 60ppm in llpumbyeo cultivar, SOD activity of copper 60ppm treatment increased in 3DAT, but there were no significant differences in 5 and 7DAT of two cultivars. Free proline contents of copper 60ppm treatment in llpumbyeo were remarkably increased about 4.996$\mu$M. In particular, free proline content of kinetin l0$^{-3}$ M in Ilpumbyeo was 5.008$\mu$M in 3DAT. In case of Hwangmibyeo 1, free proline content of copper 60ppm was 5.825$\mu$M compared with an untreated control showing 2.34l$\mu$M. The effects of kinetin treatment were recognized to promote the root growth and germination rate under copper toxicity(60ppm) condition in both cultivars.

• Journal of Life Science
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• v.28 no.7
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• pp.835-840
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• 2018

Quantitative determination of the protein expression levels is one of the most important parts in assessment of the safety of foods derived from genetically modified (GM) crops. Overexpression of AtCYP78A7, a gene encoding cytochrome P450 protein, has been reported to improve tolerance to abiotic stress, such as drought and salt stress, in transgenic rice (Oryza sativa L.). In the present study, an enzyme-linked immunosorbent assay (ELISA) kit for diagnosing AtCYP78A7 protein including AtCYP78A7-specific monoclonal antibody was developed. GST-AtCYP78A7 recombinant protein was induced and purified by affinity column. Four monoclonal antibodies (mAb 6A7, mAb 4C2, mAb 11H6, and mAb 7E8) against recombinant protein were also produced and biotinylated with avidin-HRP. After pairing test using GST-AtCYP78A7 protein and lysate of rice samples, mAb 4C2 and mAb 7E8 were selected as a capture antibody and a detecting antibody, respectively, for ELISA kit. Product test using rice samples indicated that percentages of detected protein in total protein were greater than 0.1% in AtCYP78A7-overexpressing transgenic rice (Line 10B-5 and 18A-4), whereas those in negative control non-transgenic rice (Ilpum and Hwayoung) were less than 0.1%. The ELISA kit developed in this study can be useful for the rapid detection and safety assessment of transgenic rice overexpressing AtCYP78A7.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.515-519
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• 2009

"Sangok", is a new japonica rice variety (Oryza sativa L.), which is a midium maturing ecotype developed by the rice breeding team of National Yeongnam Agricultural Experiment Station (NYAES) in 2003. This variety was derived from the cross of Milyang 101/YR8697Acp97 (in 1988/1989 winter) and selected by combination of the bulk and pedigree breeding. The pedigree of Sangokbyeo, designated as Milyang 182 in 2000, was YR12950-B-B-B-19-2-4-2-2. It has about 79cm stature in culm length and is medium maturing. This variety is resistant to bacterial blight ($K_1$, $K_2$, and $K_3$), stripe virus and moderately resistant to leaf blast disease. Milled rice kernels of "Sangok" is translucent, clear in chalkness and good at eating quality in the panel test. The yield potential of "Sangok" in milled rice is about 5.16MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to the southern plain of Korea below the Chungnam province by latitude from ordinary transplanting to transplanting after barley harvest.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.1
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• pp.1-7
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• 2005

The levels of polyamines were measured to investigate the alternative nitrogen metabolism during maturation and sprouting in rice. The rice plants (cv. Ansanbyeo) were cultivated in 20-year-old non-fertilized field. The flag leaves and spikes were collected weekly after the earing stage and the seeds were harvested daily after lodging. Free, bound, and conjugated polyamines were analyzed using reverse phase HPLC. Putrescine, spermidine, spermine, agmatine and tyramine were the major amines found in rice. The level of stress-induced amine, putrescine increased during the preharvest sprouting confirming that the process was a stress to the plants. With all other polyamines, tyramine in free form decreased in flag leaves and panicles during seed maturation. However, agmatine in bound form showed a noticeable increase about 8-fold during 6 weeks period of maturation after which it declined to the bottom level. Among the individual amines, tyramine and spermine in conjugated form showed a marked change during matutation and sprouting. Interestingly, the level of tyramine with all conjugated polyamine decreased in spikes during seed maturation and increased during preharvest sprouting implying that tyrosine decarboxyation and conjugation to phenolic acids may play a key role in preharvest sprouting. Spermine in conjugated form was synthesized only at the early earing stage in the level of $3.4mole\;g^{-1}$ fresh weight, and then decreased to the level of nmole during maturation. Thereafter, it dramatically increased to 2.8 mole during preharvest sprouting. In this study we found the tyramine is a major amine in rice, and it would play a critical role in N-assimilation during seed maturation and sprouting.