• Parasites, Hosts and Diseases
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• 제36권1호
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• pp.47-54
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• 1998

소의 주혈원충인 Reileria sp.의 small subunit ribosomal RMh (SSU rRNA) 유전자의 염 기서 열분 석을 위해 한국의 전북 장수로부터 분리하여 계대보관 중인 실험실 보관주 (KLS)와 김제 분리주 (KCB), 그리고 일본의 Shintoku 분리주 (JHS)를 실험에 사용하였다. 이들 분리주로 부터 원충을 회수 한 후 유전자를 추출하여 중합효소연쇄반응에 의 해 1.8 kb의 SSU rRNA 유전자를 증폭시 킬 수 있었으며, 증폭된 유전자를 이용하여 클론을 제작하고. 이들 클론으로 부터 플라스미드를 추출하여 유전자 염기서열분석을 실시하였다. 각 ReTheileria 분리주의 SSU rRNA유전자의 염기서열분석은 forma터와 reverse양쪽 다중복하여 실시하였으며 연속적인 primer를 이용하였다. 그 결과 한국의 소로부터 분리 한 Theue4n sp. (KLS. KCB)의 SSU rRNA유전자 염 기서 열 (Type A로 명명하였슴)은 일본 분리주와 동 일하였으며, 이 Type A를 GenBank로부터 유전자 검색을 해본 결과 Kenya의 Marula 분리주인 Reixerin buffeli의 SSU rRNA유전자 염기서열과 일치 하였다.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.515-523
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• 2001

The dinoflagellates have some primitive nuclear features and are evolutionarily intermediate between prokaryotes and eukaryotes. The small subunit ribosomal RAN gene, the 5.8S ribosomal RNA gene, and the internal transcribed spacer (ITS) of Gonyaulax polyedra were cloned, and their sequences were analyzed to better understand their evolutionary position. The small subunit ribosomal RNA gene was 1,794 nt long, the large subunit ribosomal RNA gene was approximately 3,500 nt long, and the 5.8S ribosomal RNA gene was 159 nt long. The first internal transcribed spacer (ITS1) was 191 nt long, and the second internal transcribed spacer (ITS2) was 185 nt long. The intergenic spacer of the ribosomal RNA gene (IGS) was about 2,200 nt long, indicating that 5,800 nt of transcribed sequences were separated by roughly 2,200 nt of intergenic spacer. The ribosomal RNA genes were repeated many times and arranged in a head-to-tail, tandemly repeated manner. The repeating unit of ribosomal RNA gene of G. polyedra was proposed to be 8,000 nt long. Based on the lengths of ribosomal RNA, sequence alignments with representative organisms, and phylogenetic analysis on ribosomal RNA, G. polyedra appears to be one of the alveolates branched from the eukaryotic crown and, among dinoflagellates, it seems to not have emerged early.

• 대한수의학회지
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• 제38권4호
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• pp.909-917
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• 1998

한국과 일본의 서로 다른 지역으로부터 소의 Theileria 분리주에 있어서 6가지 type(A부터 E 그리고 H)과 subtype(B1)의 small subunit ribosomal RNA(SSUrRNA) 유전자를 밝혔다. 이들 유전자 염기서열을 비교하여 본 바 염기서열의 위치 212~231, 261~270 그리고 632~690으로 3군데의 hypervariable region이 관찰되었다. SSUrRNA 유전자 염기서열 type A는 한국의 전북 분리주(KCB), 충남 분리주(KCN), 제주 분리주(KCJ)그리고 실험실 보관주(KLS)와 일본의 Shintoku 분리주(JHS)인 5개의 분리주에서 나타났으며, 이 염기서열은 Kenya의 Marula 분리주인 Theileria buffeli의 SSUrRNA 유전자(GenBank accession number Z15106)와 일치하였다. 한국의 경북 분리주(KKB)에서는 type B만이 관찰되었으나 그 외의 분리주에서는 2 type 이상의 유전자 염기서열이 관찰되었다. KCB와 JHS 분리주에서는 type A와 B, 강원 분리주(KKW)에서는 type B와 H, KCN 분리 주에서는 type A, C 및 D 그리고 KCJ 분리주에서는 type A, B, E 및 subtype B1이 관찰되었다. 한국과 일본 소의 Theileria 분리주에 있어서 여러 type의 SSUrRNA 유전자 염기서열이 나타나는 것으로 보아 혼함감염이 되어 있는 것으로 판단된다.

• 한국자원식물학회지
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• 제27권3호
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• pp.249-255
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• 2014

The rice belonging to Oryza sativa is not only has significant economic importance, for it is the major source of nutrition for about 3 billion all around the world. But also plays a vital role as a model organism, because it has a number of advantages to be a model plant, such as efficient transformation system and small genome size. Many methods and techniques have been conducted to attempt to distinguish different Oryza sativa species, such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and so on. However, studies using sequence analysis of internal transcribed spacer (ITS), a region of ribosomal RNA has not been reported until now. This study was undertaken with an aim to understand the phylogenetic relationships among sixteen isolates of Oryza sativa collected from abroad and fifteen isolates collected from Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences to compare the phylogeny relationships among different Oryza sativa species. The size variation obtained among sequenced nuclear ribosomal DNA (nrDNA) ITS region ranged from 515bp to 1000bp. The highest interspecific genetic distance (GD) was found between Sfejare 45 (FR12) and Anapuruna (FR15). Taebong isolate showed the least dissimilarity of the ITS region sequence with other thirty isolates. This consequence will help us further understanding molecular diversification in intra-species population and their phylogenetic analysis.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.87-94
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• 2007

In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slip-page heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV If-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.

• Plant Resources
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• 제8권2호
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• pp.110-115
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• 2005

Ribosomal protein complex with ribosomal RNA to form the subunits of the ribosome serve essential functions in protein synthesis. A full-length cDNA (PRPS4) encoding ribosomal protein S4 has been isolated and its nucleotide sequence determined in ginseng plant (Panax ginseng). A PRPS4 cDNA is 1105 nucleotides long and has an open reading frame of 792 bp with a deduced amino acid sequence of 264 residues (pI 10.67). The deduced amino acid sequence of PRPS4 matched to the previously reported ribosomal protein S4 genes. Their degree of amino acid identity ranged from 68 to $92\%$. Phylogenetic analysis based on the amino acid residues showed that the PRPS4 grouped with ribosomal protein S4 of S. tuberosum (CAA54095).

• 한국수산과학회지
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• 제33권2호
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• pp.103-109
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• 2000

열목어를 비롯한 산천어, 시마연어, 연어, 무지개송어 등 우리나라 연어아과 어류의 집단구조분석을 위한 기초자료를 얻기 위하여, 미토콘드리아 ribosomal RMA 유전자 영역의 염기서열변이를 비교${\cdot}$분석하였다. 미토콘드리아 DNA의 125 rRNA(945 bases, 열목어 의 경우 946 bases), Valine transfer RNA (72 bases), 및 16S rRNA(1513 bases) 등 3개의 유전자 영역에 걸쳐, 최대 2531 bases의 염기서열을 PCR/direct sequencing하여 얻었는데, 모든 염기변이중 전이가 월등히 우세하게 나타났으며, 종내${\cdot}$종간변이율은 모두 $0.5{\%}$이하로 낮게 나타나, 다른 영역에 비해 rRNA 유전자 영역에서의 염기서열이 매우 보존적임을 보여주었다. 또한, 미토콘드리아 rRNA 유전자 염기서열은 연어류의 속 (genus)단계 이상에서 집단분류표지인자로 유용하게 쓰일 수 있으리라 사료되어진다. 미토콘드리아 rRNA 염기서열자료를 기초로 구성된 phylogenetic tree를 통해 이들 종간의 진화적인 유연관계를 살펴본 결과, 시마연어가 무지개송어보다는 연어와 더 근연인 것으로 나타났으며, 열목어는 가장 유연이 먼 종임을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.67-73
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• 1998

A bacterial strain that excretes hemolysins and proteases into the growth medium was isolated from sea water and designated as KYJ 961. A nearly complete nucleotide sequence of a 16S ribosomal RNA gene from the isolate was determined following the isolation and cloning of amplified genes. On the basis of the 16S ribosomal DNA sequence data, and morphological, chemotaxonomic, and physiological characteristics, strain KYJ 961 was classified as a strain of Bacillus cereus.

• Journal of Microbiology
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• 제38권3호
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• pp.122-131
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• 2000

Phylogenetic classification of the Aphyllophorales was conducted based on the analysis of nuclear small subunit ribosomal RNA (nuc SSU rDNA) sequence. Based on phylogenetic groupings and taxonomic characters, 16 families were recognized and discussed. Although many of the characters had more or less homoplasies, miroscopic characters such ad the mitic system and clamp, spore amyloidity and rot type appeared to be important in the classification of the Aphyllophorales. Phylogenetically significant families were newly defined to improve the classification of the order Aphyllophorales.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1644-1650
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• 2013

Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.