• Applied Biological Chemistry
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• 제20권2호
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• pp.242-246
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• 1977

보리(Hordeum vulgare cultivar Sedohadaka)초엽에 $1{\times}10^{-5}M$ Gibbrellic acid 및 $1{\times}10^{-5}M$ Abscisic acid를 처리(處理)하여 RNase활성(活性)의 경시적(經時的) 소장(消長)을 무처리구(無處理區)와 비교(比較)하고, 핵산(核酸)의 형태(形態)를 관찰하였으며 그 결과(結果)를 요약(要約)하면 다음과 같다. 1) GA는 RNase의 활성(活性)을 억제(抑制)시키는 반면(反面)ABA는 촉진(促進)시켰다. 2) 무처리구(無處理區)에서 정상적인 식물(植物)에 비(比)하여 r-RNA의 비(比)가 낮고 r-RNA의 비(比)가 높았는데 이것은 배양(培養)중 RNase의 작용(作用)에 의한 것 같다. 3) GA는 r-RNA의 분해(分解)를 완화시켰으나 ABA는 촉진(促進)시켰는데 이것은 RNase의 활성(活性)과 관계된 것 같다. 4) GA는 DNA-RNA 복합체(複合體)의 합성(合成)을 촉진(促進)시켰으나 ABA는 이를 억제시켰다. 5) ABA에 의한 s-RNA의 증가(增加)는 r-RNA의 분해산물(分解産物) 때문이라 생각된다.

• 한국균학회지
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• 제12권3호
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• pp.111-116
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• 1984

Ganoderma lucidum (Fr.) Karsten을 실험재료로 하여 자실체형성 전후의 RNA를 추출 정량하였다. 버섯의 자실체 형성 전후에 걸쳐 총 RNA함량에 차이가 있었으며 자실체 형성 전에 총 RNA함량이 두드러지게 많았다. 버섯의 추출물인 RNA를 알카리가수분해한 것을 P.E.I. cellulose TLC로 전개한 결과 GMP와 XMP가 확인되었으며 미확인물질로서 한개의 spot가 나타났다. 이것은 위치로 보아 CMP가 아닌가 생각된다. 영지의 RNA추출물을 HPLC로 분석한 결과는 XMP와 GMP가 확인되었으며 그 함량은 각각, 자실체 형성 전의 GMP는 7.l9mg%, XMP는 8.43mg%, 형성후의 GMP는 4.02mg%, XMP는 3.67mg%의 함량분포를 나타내었다.

• 한국동물학회지
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• 제15권4호
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• pp.183-191
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• 1972

저자는 白色레그혼의 種卵을 孵化시켜 얻은 鷄胎呼吸氣道의 alkaline phosphatase, RNA, PAS 陽性物質 및 燐肥質의 분포와 소장을 組織化學的으로 관찰하여 다음과 같은 결과를 얻었다. 1. alkaline phosphatase는 軟骨形成에 앞서 氣道上皮 주위의 間充織에 고도의 活性을 나타냈으며 軟骨形成 後에는 反應은 감소되었고 胎生後期에는 phosphatase 活性은 上皮에만 관찰되었다. 2. RNA는 胎生초에 氣道上의 細胞質內에 陽性反應을 보였으며 軟骨形成에 앞서 上皮주위에 밀집하기 시작한 間充織에도 점차 陽性을 나타냈으나 軟骨形成후에는 이 反應은 감소하였고 鷄胎末期에 腺形成에 따라 腺上皮의 細胞質內에도 陽性 呈色反應을 보엿다. 3. PAS 陽性物質은 基底膜, 杯狀細胞 및 腺形成後에 腺內腔을 제외하고는 陰性反應을 나타냈으며 燐脂質은 發生期의 肺域내의 細氣管支上皮와 腺胞를 제외하고는 비교적 약한 呈色反應이나 陰性을 보였다.

• 대한치과교정학회지
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• 제23권3호
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• pp.415-425
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• 1993

Fluoride is one of the most potent stimulators of bone formation in vivo. But its direct effects on osteoblast is not yet clear This study was to investigate the effects of Sodium fluoride on alkaline phosphatase(ALP) activity, cAMP formation responsive to parathormone(PTH) and type I $\alpha$ 2 collagen ribonucleic acid (mRNA) level in Murin osteoblast-like (MC3T3-E1) cells. The cells were cultured in $\alpha-Minimal$ essential medium $(\alpha-MEM)$ supplemente with $10\%$ fetal bovine serum (FBS) and then changed to $0.1\%$ FBS with various concentration of Sodium fluoride. The ALP activity was assayed by the method of Lowry with disodium phenyl phosphated as substrate. cAMP formation was measured by Radioimmuno Assay(RIA). Type I $\alpha$ 2 collagen ribonucleic acid(mRNA) expression was studied by Nothern blot analysis. The results were as follows: 1. cAMP level was increased by PTH in MC3T3-E1 cells. 2. Sodium fluoride showed the tendency of inhibitory effects on cAMP responsiveness to PTH in MC3T3-E1 cells. 3. Sodium fluoride increased ALP activity at cocentration of $2{\mu}M,\;4{\mu}M,\;and\;10{\mu}M$ significantly different from control at the 0.001 level. ALP activity revealed maximum value at $10{\mu}M$ in this study. 4. Nothern blot analysis of Sodium fluoride treated cells, using Type I $\alpha$ 2 collagen prove, revealed significant increase at $10{\mu}M$ in MC3T3-E1 cells.

• 한국식물병리학회지
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• 제2권2호
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• pp.121-128
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• 1986

액독 TMV, Tw 333 RNA 및 외피단백질에 대한 생화학적 특성을 조사하였다. Tw333-RNA는 $2.03\times10^6$dalton의 분자량을 나타냈고, 고기조성은 guanine 25.4, adenine 29.2, cytosine 17.5, uracil 27.9mol이었다. 열처리에서 얻어진 이 RNA의 농색효과는 $25.1\%$를 나타냈고, 이때 Tm치는 $47^{\circ}C$였다. 한편 Tw 333의 외피단백질은 17,500 dalton의 분자량을 보였으며, 16종의 아미노산으로 구성된 158개의 아미노산잔기를 나타냈다. Trypsin으로 분해한 단백질은 9종의 ninhydrin 양성반응 peptide를 형성하였다. 이들 약독 TMV, Tw333-RNA 및 외피단백질의 생화학적 특성은 원주 OM계통과 전반적으로 매우 유사하였다. 그러나 고기조성, 농색효과, 아미노산조성 및 peptide map에서 약간의 차가 인정되었다.

• 한국균학회지
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• 제13권2호
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• pp.65-74
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• 1985

1. 분화과정에 따른 균체로부터 추출한 총 RNA량은 포자형성 직전에 증가함을 알았다. 2. 추출한 RNA를 가수분해하여 P.E.I. Cellulose TLC를 전개한 결과 AMP유도체와 IMP가 존재함을 확인하였다. 3. 포자형성을 전후하여 IMP와 AMP의 RNA에 대한 함량비율은 일정함을 알았다. 4. 포자형성 전기의 균체에 아데노신, 구아노신, 이노신, 크산토신을 처리한바 포자형성이 10배 이상 촉진되었다. 5. 포자형성 전기의 균체에 A. bisporus, F. velutipes, L. edodes의 열수추출액을 처리한바 포자형성이 $1.5{\sim}2$배 촉진되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제1권4호
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• pp.219-222
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• 1988

Degradation of deoxyribonucleic acid(DNA) and ribonucleic acid(RNA) by cell-free extract of mixed rumen protozoa of buffalo rumen was investigated. DNA was observed to be degraded rapidly during an initial incubation period of 2 hr with simultaneous appearance of degradation products. RNA on the other hand recorded a rapid degradation during an initial incubation period of 1 hr. RNA degradation products appeared upto an incubation period of 2 hr. DNA was observed to degrade into oligo- and mononucleotides. pyrimidine nucleosides, purine nucleoside adenosine and bases xanthine, hypoxanthine and thymine. Degradation products of RNA comprised of pyrimidine nucleosides, purine nucleoside, adenosine and bases xanthine, hypoxanthine and uracil besides oligo- and mononucleotides.

• Journal of Plant Biology
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• 제16권1_2호
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• pp.23-29
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• 1973

The effect of phosphorus nutrition on the content of deoxyribonucleic acid(DNA), ribonucleic acid(RNA), crude protein and plant growth of soybean plant(Glycine max, Merr.) was studied. Yields of the above-and under-ground parts of the soybean plant in terms of dry weight, the amounts of crude protein, RNA and DNA continued to increase with increasing phosphorus supply. The amounts of RNA and crude protein were highest in the leaf tissues where most intensive growth was taking place. The relationships among DNA, RNA, crude protein and plant growth appeared to consist of the central dogma which has immortalized, while DNA in plant tissue was subject to charges cuased by external environmental facters such as phosphorus nutrition.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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• pp.280-282
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• 2005

A ribonucleic acid (RNA) is one of the two types of nucleic acids found in living organisms. An RNA molecule represents a long chain of monomers called nucleotides. The sequence of nucleotides of an RNA molecule constitutes its primary structure, and the pattern of pairing between nucleotides determines the secondary structure of an RNA. Non-coding RNA genes produce transcripts that exert their function without ever producing proteins. Predicting the secondary structure of non-coding RNAs is very important for understanding their functions. We focus on Nussinov's algorithm as useful techniques for predicting RNA secondary structures. We introduce a new traceback matrix and scoring table to improve above algorithm. And the improved algorithm provides better levels of performance than the originals.

• 대한수의학회지
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• 제26권1호
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• pp.31-37
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• 1986

The present study has been carried out to elucidate the antiestrogenic effects of tamoxifen on RNA and protein synthesis in uteri of immature rats. Immature female Sprague-Dawley rats were allocated into 4 groups and injected with $5{\mu}g$ of estradiol-$17{\beta}$, $50{\mu}g$ of tamoxifen, a combination of both, or vehicle only subcutaneously three times with an interval of 24 hours respectively. The specific activities of $^3H$-uridine incorporation into uterine RNA and those of $^3H$-leucine incorporation into uterine protein were measured before and 1, 3, 6, 12, 24, 48 and 72 hours after the above treatments. The results obtained were summarized as follows; 1. Tamoxifen itself increased RNA synthesis an hour after treatment(169.18% of control), but it's specific activity was reduced to control level after 3 hours. Tamoxifen inhibited significantly (p<0.01) the activity of RNA synthesis of estradiol-$17{\beta}$. 2. The increasing rate of protein synthesis was lower in tamoxifen treated group than that in estradiol-$17{\beta}$ treated group. While the rate was steadily increased up to 357.4% of control by estradiol-$17{\beta}$ in 72 hours, tamoxifen itself failed to increase the rate after 24 hours and significantly (p<0.01) inhibited the activity of estradiol-$17{\beta}$(-167.4%).