• YAKHAK HOEJI
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• v.45 no.4
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• pp.352-356
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• 2001

A simple and rapid determination of total retinol from various pharmaceuticals containing retinol derivatives was described. Retinol derivatives were hydrolyzed with alcoholic KOH to alcoholic retinol, which was determined by HPLC. Pretreatments and HPLC conditions depend on the kind of retinols and preparation forms. The total amount of alcoholic retinol could be determined with this method from many pharmaceutical preparation of retinol derivatives. Treating time of KOH, acidic reagents and HPLC conditions were investigated.

• Proceedings of the PSK Conference
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• 2000.10a
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• pp.256.1-256.1
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• 2000

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.11
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• pp.1544-1550
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• 2008

The retinoids (vitamin A and its derivatives) play a critical role in vision, growth, reproduction, cell differentiation and embryonic development. Using the IMpRH panel, porcine cellular retinol binding protein genes 5 and 7 (RBP5 and RBP7) were assigned to porcine chromosomes 5 and 6, respectively. The complete coding sequences (CDS) of the RBP5 and RBP7 genes were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) method, and the deduced amino acid sequences of both genes were compared to human corresponding proteins. The mRNA distributions of the two genes in adult Wuzhishan pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph node, small intestine, liver, brain, kidney and fat) were examined. A total of nine single nucleotide polymorphisms (SNPs) were identified in two genes. Three of these SNPs were analyzed using the polymerase chain reaction-restriction-fragment length polymorphism (PCR-RFLP) method in Laiwu, Wuzhishan, Guizhou, Bama, Tongcheng, Yorkshire and Landrace pig breeds. Association analysis of genotypes of these SNP loci with economic traits was done in our experimental populations. Significant associations of different genotypes of $RBP5-A/G^{63}$, $RBP5-A/G^{517}$ and $RPB5-T/C^{intron1-90}$ loci with traits including maximum carcass length (LM), minimum carcass length (LN), marbling score (MS), back fat thickness at shoulder (SBF), meat color score (MCS) and hematocrit (HCT) were detected. These SNPs may be useful as genetic markers in genetic improvement for porcine production.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.4 s.48
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• pp.449-456
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• 2004

Phytoestrogens derived from plants and foods, which are diphenolic compounds with structural similarities to natural and synthetic estrogens, have been shown to estrogenic and antiestrogenic actions. Particularly, recent study revealed that phenolic compounds in safflower seed, such as serotonin derivatives, lignans and flavonoids, could be acted as phytoestrogens. Safflower (Carthamus tinctorius L.) seed extract (SID C.SE), therefore, are receiving a renewed interest as potential therapeutic source against skin wrinkles induced by estrogen deficiency. This study was conducted to investigate the anti-wrinkle effect of SID C.SE on normal human fibroblasts through the expression of type I procollagen and UVA-induced MMP-1 in vitro. The SID C.SE increased the type I procollagen expression, comparable to trans-retinol and reduced UVA-induced MMP-1 expression in a dose-dependent manner. The clinical study indicated that cream group treated with $0.1\%$ SID C.SE significantly reduced a skin wrinkles, as compared with a control (non-treated cream group) (p<0.05). These results suggest that the safflower seed extract may be useful as potential source of anti-wrinkle cosmetics.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.1 s.55
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• pp.35-44
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• 2006

Recently, encapsulation studies have been tarried out to protect active agents using shell materials such as polymers, lipids, inorganic materials and the other protective materials. We have prepared copolymers of methylmethacrylate (MMA) and trimethoxysilylpropylmethacrylate (TMPMA), and the copolymers as shell materials were used for encapsulating active agents. Poly(MMA-co-TMPMA) spheres were very efficient for encapsulating active agents such as vitamin derivatives (such as retinol, retinyl palmitate, tocopheryl acetate and ascorbyl tetraisopalmitate) and oil soluble licorice extract etc. Mean diameters of poly(MMA-co-TMPMA) core-shell spheres containing active agents varied between about 0.1 to $10{\mu}m$ according to the experimental conditions. The loading amount of encapsulating active agents was 15 to 25% (w/w) and the loading yield was above 90%. The stability of active agents in poly(MMA-co-TMPMA) core-shell spheres prepared with an UV absorbing precursor increased by 25% compared with that of active agents in spheres prepared without an UV absorbing precursor.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.405-408
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• 2004

Several hydroxamic acid derivatives are synthesized to observe retinoidal effect and transactivation potential for $RAR{\alpha}/{\gamma]$ is screened. Among the synthesized compounds, N-(4-N-hydroxycarbamoyl )phenyl) [4-(tert-butyl )phenyl] carboxamide (2f) showed the best compatibility for potent $RAR{\alpha}/{\gamma].$ The acidic hydroxy of hydroxamic acid was easily deprotonated to form an enolate. A formed enolate has a similar role like that of all-trans-retinoic acid. This is the first example of the hydroxamic acid derivative with retinoidal activity. The retinoidal activity of 2f was further confirmed by enhancing activity for the expression of retinoid-responsive genes. To evaluate the possibility for anti.-aging agent, effect on the expression of MMP-1 was measured comparing with all-trans-retinoic acid and retinol. At 10 uM treatment, compound 2f inhibited the expression of MMP-1. These results suggest that new hydroxamic acid derivative 2f could be used as a promising anti-aging agent.