• Korean Journal of Food Science and Technology
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• v.39 no.5
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• pp.506-514
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• 2007

In this study, carrot juice was treated with high voltage pulsed electric fields (PEF) and the changes in its physical and chemical properties during storage at $4^{\circ}C$ and $25^{\circ}C$ were investigated. The sterility fur bacteria, yeast and mold in carrot juice increased with increasing electric field strength and treatment temperature. While yeast and mold were completely inactivated at 65kV/cm with a treatment time of $200{\mu}s$ in a continuous PEF treatment system, bacteria were reduced by four log cycles. The results also showed that square wave pulse treatment was more effective for inactivating microorganisms than exponential decay pulse, and this effect was more apparent for carrot juice of lower pH. Although we observed significant changes in physical and chemical properties such as soluble solid content, pH, acidity, color, and carotene retention when the PEF treated samples were stored at the ambient temperature $(20^{\circ}C)$, no significant physical and chemical changes were found at the cold storage temperature $(4^{\circ}C)$ during 28 days of storage. The results indicate that the PEF treated carrot juice is appropriate for commercial refrigerated storage.

• Journal of Oriental Neuropsychiatry
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• v.9 no.2
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• pp.37-44
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• 1998

Purpose: This study was conducted to find out the effects od Sahyangsohapwon on learning and memory of rats.Method: In the experiment, rats were divided two groups. One was control group which was adminstered Sahyangsohapwon and the other was sample group administered placebo. Numbers of each group were 13 rats. 8-arm radial maze task was used in it, and working memory test and retention(reference memory) test were done.Before the beginning of the test, the rats were deprived of water for 24hrs.In the frist test, each of eight arm was baited with water and a rat was permitted to remain on the maze until all eight arms were entered. A working memory error was defined as revisit of any previously entered arm. When a rat made an error not exceeding one time in consecutive 3 days-performance, it was regarded as learning criteria and the test was ended. The reference memory was evaluated with total days which it took rats to pass the learning crtirtia.The second test was performed after 24 hours when the first test was over. When a rat entered 4 arms, the entrance of arm was cut off during 30 seconds.Here the number if errors which was produced during a rat find remaining 4 tracks was regarded as the index of memory.This experiment compared the number of error at the control group with that of the sample group.Result: 1. In the first test, it was shown that the sample group took 7.69${\pm}$1.11 days and the control group 9.31${\pm}$1.97 days to pass the learning criteria.There was statistically significant reference mernory development at the sample group.2. In the second test, the frequency of errors made by the two groups were 0.92${\pm}$1.32 times for the control group and 1.23${\pm}$1.59 times for the Sahyangsohapwon group. There was no difference between the groups in frequency of errors.Conclusion: It is suggested that Sahyangsohapwon has effects on the improvement of learning and memory.

• Journal of Korea Soil Environment Society
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• v.4 no.3
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• pp.45-56
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• 1999

A biofilter was established to remove the ammonia, which is representative nitrogen-contained malodorous gas. in a compost factory. Removal efficiency of ammonia and hydrogen sulfide also was investigated. A quantity of malodor gas produced in a compost factory was affected greatly by the weather. compost states and working condition of a fertilizing mixer, and the produced gas concentrations doubled by above various parameters. By operating a water scrubbing system for removing water-soluble malodorous gases effectively. we could improve the removal efficiency over three times. We investigated long-term stability of biofilter under continuous gas flow(SV=500h-1) for 100 days. The results showed 30 days of microbial retention time. After the days, deodorization efficiency of biofilter was kept steady state. and the removal efficiency was kept over 95% for ammonia and 97% for hydrogen so]fide. respectively. The electric consumption of the biofilter, which could treat malodorous gas of 100$\textrm{m}^3$/min, applied in the compost factory was evaluated about 80u0day and water consumption was 80~100$\ell$/day. These results concluded that the biofilter is a excellent deodorization technology as well as cost-effective for removing malodorous gas produced in a compost factory.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.4
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• pp.181-192
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• 2003

We developed a mathematical simulation model to portray the vertical distribution of soil water from the measured weather data and the known soil hydraulic properties, and then compared simulation results with the periodically measured soil water profiles obtained on Jungdong sandy loam to verify the model, In this model, we solved potential-based Richards' equation by the implicit finite difference method superimposed on the predictor-corrector scheme. We presumed that: soil hydraulic properties are homogeneous; soil water flows isothermally; hysteresis is not considered; no vapor flows; no heat transfers into the soil profiles; and water added to soil surface is distributed along the soil profile following partial displacement principle. The input data were broadly classified into two groups: (1) daily weather data such as rainfall, maximum and minimum air temperatures, relative humidity and solar radiation and (2) soil hydraulic data to approximate unsaturated hydraulic conductivity and water retention. Each hydraulic polynomial function approximated using the Chebyshev polynomial and least square difference technique in tandem showed a fairly good fit of the given set of data. Vertical distribution of soil water as approximations to the Richards' equation subject to changing surface and phreatic boundaries was solved numerically during 53 days with a comparatively large time increment, and this pattern agreed well with field neutron scattering data, except for the surface 0.1 m slab.

• Korean Journal of Microbiology
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• v.34 no.3
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• pp.126-131
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• 1998

Reduction of hexavalent chromium to its trivalent form by leachate microorganisms was studied in batch and bench-scale continuous stirred tank reactor. The inoculum was a culture of microorganisms in leachate and capable of providing up to 90% chromate reduction during 72 h batch assay with $20mg\;Cr(VI)\;L^{-1}$ in minimal media containing different levels of leachate (10 to 60%) and glucose (50 to 200 mM). Addition of glucose increased the efficiency of chromate reduction, but adverse effect was observed with increase of leachate probably due to the competitive inhibition between chromate and sulfate ions. The continuous culture experiment was conducted for 124 days using synthetic feed containing different levels of chromate (5 to $65mg\;L^{-1}$) at room temperature. With a hydraulic retention time of 36 h, chromate reduction efficiency was mostly 100% when Cr(VI) concentrations in the reactor were in the range of 5 to $50mg\;L^{-1}$ Specific rate of Cr(VI) removal was calculated as $3.492mg\;g^{-1}\;protein\;h^{-1}$ during the period of 101~124 days from the start-up which showed 81.2% of average reduction efficiency. The results indicate the potential application of using leachate microorganisms for detoxification of hexavalent chromium in various chromium-contaminated wastewater from landfill or tannery sites.

• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.156-160
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• 1997

The combination of biological wastewater treatment process and membrane separation has many advantages such as better effluent quality and system stability over the conventional biological wastewater treatment process. In this study, direct membrane separation using nonwoven fabric was applied to biological wastewater treatment. A nonwoven fabric module was submerged in the aerated bioreactor. And accumulated biomass in the bioreactor was separated by suction. The system was operated with various condition to investigate pollutant removal efficiencies and flux. After formation of biomass layer on nonwoven fabric surface, a day, the stable effluent water quality was obtained. The flux decreased at a high suction pressure faster than a low pressure. The stable flux was obtained at the pressure of $21{\sim}25cmHg$. In spite of variation of hydraulic retention time, organic loading rate, the removal efficiencies of BOD, $COD_{Cr}$. $COD_{Mn}$ were very high as follows : $95.2%(0.14{\sim}0.97\;BODKg/m^3/day)$, $86.0%(0.17{\sim}1.39\;COD_{Cr}Kg/m^3/day)$, $90.0%(0.097{\sim}0.61\;COD_{Mn}Kg/m^3/day)$.

• Biomolecules & Therapeutics
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• v.23 no.6
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• pp.597-603
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• 2015

Synthetic cannabinoids JWH-018 and JWH-250 in 'herbal incense' also called 'spice' were first introduced in many countries. Numerous synthetic cannabinoids with similar chemical structures emerged simultaneously and suddenly. Currently there are not sufficient data on their adverse effects including neurotoxicity. There are only anecdotal reports that suggest their toxicity. In the present study, we evaluated the neurotoxicity of two synthetic cannabinoids (JWH-081 and JWH-210) through observation of various behavioral changes and analysis of histopathological changes using experimental mice with various doses (0.1, 1, 5 mg/kg). In functional observation battery (FOB) test, animals treated with 5 mg/kg of JWH-081 or JWH-210 showed traction and tremor. Their locomotor activities and rotarod retention time were significantly (p<0.05) decreased. However, no significant change was observed in learning or memory function. In histopathological analysis, neural cells of the animals treated with the high dose (5 mg/kg) of JWH-081 or JWH-210 showed distorted nuclei and nucleus membranes in the core shell of nucleus accumbens, suggesting neurotoxicity. Our results suggest that JWH-081 and JWH-210 may be neurotoxic substances through changing neuronal cell damages, especially in the core shell part of nucleus accumbens. To confirm our findings, further studies are needed in the future.

• Natural Product Sciences
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• v.15 no.3
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• pp.144-150
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• 2009

Gradient centrifugal partition chromatography (GCPC) method was developed and applied to isolate 3,5-dimethoxyphenanthrene-2,7-diol (DMP) and batatasin-I (BA-I) from the dichloromethane soluble extract of Dioscorea opposita. In this method, the lower phase of n-hexane-methanol-water system (HMW, 10 : 9 : 1, v/v) was used as a mobile phase A (MpA) and water was used as a mobile phase B (MpB). This gradient CPC method is comparable to that of reversed-phase HPLC method in that the stationary upper-phase of HMW (10 : 9 : 1 v/v) works as if it were reversed-phase silica gel due to its hydrophobic property, while the lower phase (MpA) and water (MpB) functioned as hydrophilic mobile phases. The initial condition of the mobile phase was 20% MpA/80% MpB and maintained for 150 min to obtain DMP (1.2 mg), and then MpA was increased up to 50% to elute BA-I (1.7 mg). The purities of DMP and BA-I were 94.1% and 98.3% with the recovery yields of 83% and 86%, respectively. Similar results were obtained by linear-gradient CPC. The CPC peak fractions were identified by comparing their retention time to those of authentic samples of DMP and BA-I and their spectroscopic data ($^1$H NMR and $^{13}$C NMR) to those of literature values.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.3
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• pp.337-345
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• 2014

The present study examined the effects of soybean-derived phosphatidylserine (SB-PS) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The cognitive improving efficacy of SB-PS on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing cholineacetyl transferase (ChAT), acetylcholinesterase (AChE) and cAMP responsive element binding protein (CREB) immunohistochemistry. A positron emission tomography (PET) scanning the rat brain was by performed administer 18F-Fluorodeoxy-glucose (18F-FDG). The rats with TMT injection showed impaired learning and memory of the tasks and treatment with SB-PS produced a significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd day compared to that of the MCT group. In the retention test, the SB-PS group showed increased time spent around the platform compared to that of the MCT group. Consistent with the behavioral data, SB-PS 50 group significantly alleviated the loss of acetyl cholinergic neurons in the hippocampus compared to that of the MCT group. Treatment with SB-PS significantly increased the CREB positive neurons in the hippocampus as compared to that of the MCT group. In addition, SB-PS groups increased the glucose uptake in the hippocampus and SB-PS 50 group increased the glucose uptake in the frontal lobe, as compared to that of the MCT group. These results suggest that SB-PS may be useful for improving the cognitive function via regulation of cholinergic marker enzyme activity and neural activity.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.350-356
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• 2011

The purpose of this study was to investigate changes in the contents of constituents in Sipjundaebo-tang (SJ) and its fermentations (FSJ) with 8 species of lactic acid bacteria. Eight strains of lactic acid bacteria, Lactobacillus casei KFRI129, L. plantarum KFRI 144, L. curvatus KFRI 166, L. hilgardii KFRI 229, L. delbruekil subsp. lactis KFRI 442, L. casei KFRI 692, L. gasseri KCTC 3163, and Bifidobacterium breve KFRI 744, were utilized for the fermentation of Sipjundaebo-tang. As a result we identified three constituents which increased and two constituents which decreased. The increased constituents were isolated by chromatographic techniques and then their structure elucidated using NMR and MS. The decreased constituents were confirmed by comparing standard compounds. These compounds were quantitatively analyzed using the HPLC/DAD system. The increased constituents were identified to be cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5), while the decreased constituents were liquiritin (2) and nodakenin (4). Generally, liquiritin (2) and nodakenin (4) were noted as having decreased in fermented Sipjundaebo-tangs (FSJs), while cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5) were seen to have increased. Sipjundaebo-tang fermented by L. plantarum KFRI144 exhibited the most remarkable changes in all of fermentations. The eight lactic acid bacteria all demonstrated differing decomposable rates on the five maker compounds in fermented Sipjundaebo-tang (FSJ).