• 한국연초학회지
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• 제37권1호
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• pp.25-33
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• 2015

Genome walking is a par ticular application for identifying sequences of unknown genomic regions adjacent to a known region. Many genome walking methods based on polymerase chain reaction (PCR) are available. Even if earlier techniques suffer from low reproducibility, inefficiency, and non-specificity, improved strategies have been developed. In this study, we present an alternative strategy: the genomic DNA is digested with restriction enzymes. After cytosine overhangs at 5' ends, the fragments are ligated to linker adaptor s had guanine overhang at 3' ends. Then nested PCR is performed. The improvements in this strategy focus on two points. The first is the C tailing method using Pfu polymerase instead of the A tailing method based on nontemplate-dependent terminal transferase activity of Taq polymerase. Therefore unintended modification of target DNA can be prevented without A tailing error. The second point is the use of C/G-specific ligation had advantage in the ligation efficiency compared with A/T-specific ligation. Therefore, the C-G linker PCR method increases ligation efficiency between digested genomic DNA and adaptor DNA. As a result, the quantity of target DNA to amplify by PCR is enriched. We successfully used G-C linker PCR to retrieve flanking regions bordering the phophinothricin resistance gene in genetically modified tobacco (GMO).

• 한국비블리아학회지
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• 제12권2호
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• pp.49-70
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• 2001

멀티미디어기술의 발전과 더불어 타인의 저작물에 대한 이용의 기대나 필요성이 커지고 있는 만큼 저작권이나 저작권법과의 충돌이나 접촉의 잠재적 가능성이 증대하고 있다. 특히 멀티미디어환경에서는 종래와 비교하여 저작물의 복제가 용이하고, 복제물의 품질이 뛰어나며, 저작물의 자유로운 변경이 용이하다고 하는 특징이 있다. 또한 멀티미디어환경에서의 저작물은 복합된 저작물이 많고, 인터랙티브한 저작물로서 전통적인 저작물의 특성으로부터 이탈하는 성격을 지니고 있다. 다른 한편, 정보에의 자유로운 접근이라고 하는 멀티미디어의 특성에 의하여 타인의 저작물의 자유로운 이용이라고 하는 과제를 해결하여야 할 필요가 있다. 결국 종래의 저작전법의 멀티미디어환경에서의 적응을 위하여는 새로운 변화가 요구되며, 해결되어야 할 과제가 많다고 본다.

• Journal of Electrical Engineering and Technology
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• 제3권3호
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• pp.354-362
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• 2008

In this paper, a new algorithm of optimal voltage control is proposed for the Distribution Automation System (DAS) based on constants of four terminal network modeling. In the proposed method, the voltage profiles along feeders are estimated from the measurement of the current and power factor by a Feeder Remote Terminal Unit (FRTU) installed at each node. Whenever the voltage profile violates the restriction, the voltage control strategy is applied to keep the voltage levels along the feeders within the pre-specified range through the modification and coordination of the transformer under-load tap changers (ULTC), step voltage regulator (SVR), as well as shunt condenser. In the case studies, the estimation and control of the voltages have been testified in a radial distribution system with 11 nodes.

• 재활간호학회지
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• 제13권1호
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• pp.62-69
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• 2010

Purpose: The purpose of this study was to evaluate the effects of an integrative weight control program including East Asian traditional medicine on the degree of obesity and body compositions. Method: Using one group pre-post test design, 63 subjects aged 19 years or older were conveniently recruited from D University hospital. The weight control program consisted of acupuncture, aerobic exercise, moderate caloric restriction, and behavioral modification for weight loss. Paired t-tests were conducted using the SPSS V18.0. Results: Body weight (t=10.44, p<.001), body fat mass (t=11.47, p<.001), percentage of body fat (t=11.49, p<.001), the degree of obesity (t=10.48, p<.001), body mass index (t=10.66, p<.001), waist circumference (t=10.25, p<.001), hip circumference (t=6.10, p<.001), and WHR (t=2.21, p=.040) decreased after administering the integrative weight control program. Conclusion: This integrative weight control program effectively reduced the obesity degree and percentage of body fat. Further study is needed to replicate our program in a larger sample with control group to validate the findings.

• 산업공학
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• 제11권2호
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• pp.211-225
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• 1998

As mentioned in Part I all groupware products have been categorized into three areas which include cooperation/document management systems(CMS), collaborative writing systems(CWS), and decision-making/meeting system(DMS). This study deals with a comparative analysis of the last two areas, which is added to the first. It turns out that DMS has a higher market share than CWS. However. since effective collaboration requires the functions inherent to these two systems. they should be integrated somehow. The systems' functions that have been implemented in response to design issues have been described. Each group of the functions has been divided into three parts which consist of basic function, quasi-basic function. and others. Such a decision has been made according to the frequency rate of the functions provided in the products. While the basic functions in CWS include collaboraive writing beyond restriction of time and place, group awareness. version control. and others, in DMS realtime collaboration. brainstorming. presentation. various task support. policy formation. document management, multimedia, subgroup communication. topic commenter, categorizer, screen capture and various rile transfer. The basic functions are merged into the integrated functional model which was proposed in Part I. Since the model is so flexible that it can partially include the quasi-functions in addition to the hasic functions. a large number of products may stem from the modification of the functional model.

• Genomics & Informatics
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• 제15권2호
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• pp.69-80
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• 2017

In this study, cytolethal distending toxin (CDT) producer isolates genome were compared with genome of pathogenic and commensal Escherichia coli strains. Conserved genomic signatures among different types of CDT producer E. coli strains were assessed. It was shown that they could be used as biomarkers for research purposes and clinical diagnosis by polymerase chain reaction, or in vaccine development. cdt genes and several other genetic biomarkers were identified as signature sequences in CDT producer strains. The identified signatures include several individual phage proteins (holins, nucleases, and terminases, and transferases) and multiple members of different protein families (the lambda family, phage-integrase family, phage-tail tape protein family, putative membrane proteins, regulatory proteins, restriction-modification system proteins, tail fiber-assembly proteins, base plate-assembly proteins, and other prophage tail-related proteins). In this study, a sporadic phylogenic pattern was demonstrated in the CDT-producing strains. In conclusion, conserved signature proteins in a wide range of pathogenic bacterial strains can potentially be used in modern vaccine-design strategies.

• Journal of Animal Science and Technology
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• 제65권5호
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• pp.1105-1109
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• 2023

Pedi coccus acidilactici CACC 537 was isolated from canine feces and reported to have probiotic properties. We aimed to characterize the potential probiotic properties of this strain by functional genomic analysis. Complete genome sequencing of P. acidilactici CACC 537 was performed using a PacBio RSII and Illumina platform, and contained one circular chromosome (2.0 Mb) with a 42% G + C content. The sequences were annotation revealed 1,897 protein-coding sequences, 15 rRNAs, and 56 tRNAs. It was determined that P. acidilactici CACC 537 genome carries genes known to be involved in the immune system, defense mechanisms, restriction-modification (R-M), and the CRISPR system. CACC 537 was shown to be beneficial in preventing pathogen infection during the fermentation process, help host immunity, and maintain intestinal health. These results provide for a comprehensive understanding of P. acidilactici and the development of industrial probiotic feed additives that can help improve host immunity and intestinal health.

• Clinical and Experimental Reproductive Medicine
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• 제43권2호
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• pp.82-89
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• 2016

Objective: The long interspersed elements (LINE-1, L1s) are a group of genetic elements found in large numbers in the human genome that can translate into phenotype by controlling genes. Growing evidence supports the role of epigenetic in polycystic ovary syndrome (PCOS). The purpose of this study is to evaluate the DNA methylation levels in LINE-1 in a tissue-specific manner using cumulus cells from patients with PCOS compared with normal controls. Methods: The study included 19 patients with PCOS and 22 control patients who were undergoing controlled ovarian hyperstimulation. After oocyte retrieval, cumulus cells were extracted. LINE-1 DNA methylation levels were analysed by bisulfite treatment, polymerase chain reaction, and restriction enzyme digestion. The Connection Up- and Down-Regulation Expression Analysis of Microarrays software package was used to compare the gene regulatory functions of intragenic LINE-1. Results: The results showed higher LINE-1 DNA methylation levels in the cumulus cells of mature oocytes in PCOS patients, 79.14 (${\pm}2.66$) vs. 75.40 (${\pm}4.92$); p=0.004, but no difference in the methylation of cumulus cells in immature oocytes between PCOS and control patients, 70.33 (${\pm}4.79$) vs. 67.79 (${\pm}5.17$); p=0.155. However, LINE-1 DNA methylation levels were found to be higher in the cumulus cells of mature oocytes than in those of immature oocytes in both PCOS and control patients. Conclusion: These findings suggest that the epigenetic modification of LINE-1 DNA may play a role in regulating multiple gene expression that affects the pathophysiology and development of mature oocytes in PCOS.

• 컴퓨터교육학회논문지
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• 제8권1호
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• pp.73-80
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• 2005

변형 가능한 물체들 간의 자연스럽고 그럴듯한 상호작용을 얻거나, 시뮬래이션을 사용자가 원하는 시작 조건으로 설정하기 위해서는 이를 제어할 수 있는 기하학적인 제한을 직관적으로 정의하거나 제어 할 수 있어야 한다. 또한 사용자가 시뮬레이터의 중대한 변경 없이 시뮬레이션을 다양한 환경의 시뮬레이션문제를 풀기위한 기반으로 사용할 수 있어야 한다. 본 논문에서 제안된 물리학 기반의 기하학적 제한 시뮬레이션 시스템은 변형 가능한 물체를 표현하기 위혜서 비선형적인 유한요소 해석 방법을 사용하였으며, 제약 조건을 지키기 위해서 물체의 노드에 기하학적인 제한을 설정함으로써 제한 힘이 생성된다. 또한 사용자가 기하학적인 제한을 설정하고 변경 할 수 있도록 해주며, 이러한 제약들은 지속적으로 시뮬레이션 시스템을 통제 할 수 있도록 제한 힘으로 변환된다. 따라서 시뮬레이터는 물체에 적용되는 선형적, 각도, 부동식 등의 기하학적 제한을 통제 할 수 있다. 본 연구의 실험적인 결과들은 전체 시뮬레이션 동안 기하학적 제한이 작은 오차 범위 내에서 잘 지켜지고, 변형 가능한 물체의 원하는 형태를 잘 보존하고 있음을 보여준다.

• 대한소아치과학회지
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• 제26권1호
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• pp.32-37
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• 1999

항시 장착이 가능한 reverse twin block 장치에 기능의 장애를 받지 않고도 lip pad의 효과를 첨가하기 위하여 가철식으로 lip pad를 제작하여 III급 부정교합의 치료에 적용해 본 결과 다음과 같은 결론을 얻었다. 1. Reverse twin block 장치는 장착한 상태에서 lip pad의 착탈을 통하여 정상적인 저작, 발음 등의 기능을 할 수 있으며 심미적이므로 III급 부정교합의 치료에 있어 상하악이 분리되어 있지 않은 다른 기능성 장치가 가진 단점을 극복할 수 있었다. 2. 본 증례에서는 저발육된 상악에 대한 효과적인 작용을 하는 lip pad를 가철식으로 제작하여 기능의 장애를 받지 않고도 양호한 치료효과를 얻을 수 있었다.